Bacterial interactions of microplastics extracted from toothpaste under controlled conditions and the influence of seawater.

Microplastics have become a global concern due to their increasing use and discharge into the environment. These ubiquitous particles are known to have extremely low degradation rates and accumulate mostly in the marine environment. The evidence for bioaccumulation and indicators of stress linked to microplastics is also stated in the literature. However, the real environmental impact of microplastics has not yet been revealed. Therefore, it is crucial to understand the interaction mechanisms between microplastics and (micro)organisms under controlled (standard) laboratory conditions and environmentally relevant conditions to reflect the true environmental -situation. In this study, we aimed to understand how microplastics extracted from commercially available toothpaste samples interacted with four types of bacteria under both standard and seawater conditions. For this purpose, bacterial inhibitions were examined, and mechanisms of inhibition were evaluated by biochemical parameters (total protein, lipid peroxidase, total antioxidant capacity, and extracellular carbohydrate levels) of bacteria and physicochemical properties (zeta potential, particle size, surface chemistry) of microplastics. Results showed that gram-positive Bacillus subtilis and gram-negative Pseudomonas aeruginosa were affected in controlled and sea water media, respectively. The inhibition of the bacteria relied on the high zeta potentials of the microplastics, and, biochemically, protein and lipid peroxidase activity of bacteria were important in both media. On the other hand, while biochemical responses were similar in both media, the difference between the cell wall and microplastics surface charge was important only in seawater.

The use of urinary fluoride excretion to facilitate monitoring fluoride intake: A systematic scoping review.

BACKGROUND: As a recognised effective and economical agent for dental caries prevention, fluoride has been used in many different fluoridation schemes implemented across the world. Considering the narrow 'dose-gap' between the benefit of caries reduction and the risk of dental fluorosis, it is recommended that fluoride intake is monitored by measuring urinary fluoride excretion. The aim of this scoping review is to map the current literature/evidence on fluoride intake and excretion studies in relation to the study population, settings, type of study design, methodology, and analytical approach. METHODS: Embase/Ovid, MEDLINE/Ovid, CINAHL/EBSCO, Scopus/Elsevier were searched for relevant articles until April 2018. Studies were included if they reported intake and excretion of fluoride in healthy humans of all age groups. Findings were explored using a narrative synthesis to summarise studies characteristics and outcome measures. RESULTS: Removal of duplicates from the originally 2295 identified records yielded 1093 studies of which 206 articles were included. Only 21.6% of the studies were conducted in children (<8-year-olds). Most studies (38.8%) used drinking water concentration as a proxy for fluoride intake, whereas only 11.7% measured fluoride intake from all sources. Of the 72 studies that measured dietary fluoride intake, only 10 reported the validity of the employed dietary assessment method. Only 14 studies validated the urine sample collection methods. No information on the validity of the employed analytical method was reported by the majority (64.6%) of studies. Only a small proportion (8.7%) of the included studies investigated the association between fluoride intake and excretion. CONCLUSION: The findings reveal much variability in terms of conducting the studies and reporting the findings, illustrating a high heterogeneity in data collection across settings and populations. Future studies should provide more detail on sampling technique, measurement protocols (including validation), and on clearly defining the relationship between intake and urinary excretion of fluoride.