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Sci Rep ; 7(1): 12345, 2017 09 27.
Article in English | MEDLINE | ID: mdl-28955045

ABSTRACT

Taeniids exhibit a great adaptive plasticity, which facilitates their establishment, growth, and reproduction in a hostile inflammatory microenvironment. Transforming Growth Factor-ß (TGFß), a highly pleiotropic cytokine, plays a critical role in vertebrate morphogenesis, cell differentiation, reproduction, and immune suppression. TGFß is secreted by host cells in sites lodging parasites. The role of TGFß in the outcome of T. solium and T. crassiceps cysticercosis is herein explored. Homologues of the TGFß family receptors (TsRI and TsRII) and several members of the TGFß downstream signal transduction pathway were found in T. solium genome, and the expression of Type-I and -II TGFß receptors was confirmed by RT-PCR. Antibodies against TGFß family receptors recognized cysticercal proteins of the expected molecular weight as determined by Western blot, and different structures in the parasite external tegument. In vitro, TGFß promoted the growth and reproduction of T. crassiceps cysticerci and the survival of T. solium cysticerci. High TGFß levels were found in cerebrospinal fluid from untreated neurocysticercotic patients who eventually failed to respond to the treatment (P = 0.03) pointing to the involvement of TGFß in parasite survival. These results indicate the relevance of TGFß in the infection outcome by promoting cysticercus growth and treatment resistance.


Subject(s)
Cysticercus/immunology , Host-Parasite Interactions/immunology , Neurocysticercosis/immunology , Taenia solium/immunology , Transforming Growth Factor beta/immunology , Activin Receptors/genetics , Activin Receptors/immunology , Activin Receptors/metabolism , Animals , Antigens, Helminth/genetics , Antigens, Helminth/immunology , Antigens, Helminth/metabolism , Antiparasitic Agents/pharmacology , Antiparasitic Agents/therapeutic use , Cysticercus/genetics , Cysticercus/metabolism , Disease Models, Animal , Drug Resistance/immunology , Genome, Helminth/immunology , Helminth Proteins/genetics , Helminth Proteins/immunology , Helminth Proteins/metabolism , Humans , Mice , Mice, Inbred BALB C , Neurocysticercosis/cerebrospinal fluid , Neurocysticercosis/drug therapy , Neurocysticercosis/parasitology , Signal Transduction/immunology , Swine , Taenia solium/genetics , Taenia solium/metabolism , Transforming Growth Factor beta/cerebrospinal fluid , Transforming Growth Factor beta/metabolism
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