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Mar Genomics ; 33: 21-25, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28237778

ABSTRACT

De-novo assembly of a metagenomic dataset obtained from a decaying cyanobacterial Trichodesmium bloom from the New Caledonian lagoon resulted in a complete giant phage genome of 257,908bp, obtained independently with multiple assembly tools. Noteworthy, gammaproteobacteria were an abundant fraction in the sequenced samples. Mapping of the raw reads with 99% accuracy to the giant phage genome resulted in an average coverage of 262X. The closest sequenced relatives, albeit still distant, are the Pseudomonas phages PaBG from Lake Baikal and Lu11 isolated from a soil sample from the Philippines. The phage reported here might belong to the same family within the Myoviridae as PaBG and Lu11 and would thus be its first marine member, indicating a more widespread occurrence of this group. We named this phage NCTB (New Caledonia Trichodesmium Bloom) after its origin.


Subject(s)
DNA, Viral/genetics , Genome, Viral , Myoviridae/genetics , Trichodesmium/virology , Eutrophication , Gammaproteobacteria/virology , Metagenomics , Myoviridae/isolation & purification , New Caledonia
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