Diagnostic features of metastatic endometrioid carcinoma in a captive black-tufted marmoset (Callithrix penicillata).

A captive marmoset developed metastatic endometrioid carcinoma (EnC), a rare uterine tumor in non-human primates (NHPs). The neoplasm showed marked microscopical malignant and tubulopapillary aspects, immunopositivity for pan-cytokeratin, CK7, estrogen receptor, and a high mitotic index (Ki-67). These features may contribute to the diagnosis and therapeutics of EnC in NHPs.

Bilateral pyelonephritis due to Escherichia coli infection in a captive jaguar (Panthera onca) / Pielonefrite bilateral por Escherichia coli em uma onça-pintada (Panthera onca)

Extraintestinal pathogenic Escherichia coli (ExPEC) is a highly diverse pathotype of E. coli which colonizes the intestine, and it is considered an important etiological agent associated with bacteremia and other systemic infections, among them urinary tract infection. Retrospective studies evaluating morbidity and mortality of nondomestic felids have demonstrated that urinary tract diseases are among the main causes of death for geriatric animals. Also, mesenchymal neoplasms of the uterus are common in wild felids, and they possess variable morphologic characteristics related to invasiveness and malignancy. This report describes a case of bilateral pyelonephritis due to extraintestinal uropathogenic E. coli infection in a captive jaguar (Panthera onca). The diagnosis was confirmed through pathological, bacterial and immunohistochemical findings. According to molecular analysis, this E. coli strain was classified in the phylogroup F, possessing the following virulence-associated genes: usp, cnf-1, hlyA, papC and sfa. Additionally, this E. coli was highly resistant to beta-lactams and first-generation cephalosporin. This jaguar also presented a uterine leiomyoma with distinct distribution, and severe degenerative articular disease, both of them described as frequently seen lesions in geriatric animals from the Panthera genus.(AU)

Escherichia coli extraintestinal patogênica (ExPEC) é um patotipo altamente diverso de E. coli que coloniza o intestino e é considerada um agente etiológico importante, associado com bacteremia e outras infecções sistêmicas, dentre elas infecções do trato urinário. Estudos retrospectivos avaliando morbidade e mortalidade de felídeos não domésticos demostram que doenças do trato urinário estão entre as principais causas de morte de animais geriátricos. Ainda, neoplasias mesenquimais uterinas são comuns em felídeos de cativeiro e possuem características morfológicas variáveis relacionadas a invasividade e malignidade. Neste relato é descrito um caso de pielonefrite bilateral por E. coli extraintestinal uropatogênica em uma onça-pintada de cativeiro (Panthera onca). O diagnóstico foi confirmado através dos achados patológicos, bacteriológicos e imuno-histoquímicos. A partir da análise molecular, esta cepa de E. coli foi classificada no filogrupo F, possuindo os seguintes genes associados a virulência: usp, cnf-1, hlyA, papC and sfa. Adicionalmente, a bactéria isolada foi altamente resistente a ß-lactâmicos e cefalosporinas de primeira geração. Foi observado ainda um leiomioma uterino com distribuição distinta e doença articular degenerativa severa, ambas descritas na literatura como comumente observadas em animais geriátricos do gênero Panthera.(AU)

The evaluation of clinicopathological and immunohistochemical findings of cervical clear cell carcinoma in a bitch

Background: Clear cell carcinoma (CCC) of the cervix is a rarely seen malignant tumor which is classified in adenocarcinomas of the endometrium. It is responsible for 2.15-3.0% of endometrial tumors. The diagnosis is difficult and is still controversial in uterine carcinomas. Macroscopically, masses were protruding to endometrial surface. However, the definitive diagnosis was carried by microscopical evaluation. The cells contain clear cytoplasm. The cells are seen in this pattern because there is either glycogenation or secretoric activity in the cells.Case: In the present case, clinicopathologic and immunohistochemical findings of clear cell carcinoma of the cervix were defined in a 3 year-old spayed Golden retriever bitch. Previously spayed bitch, which had vaginal discharge for the last month-long, was submitted to the Department of Obstetrics and Gynecology, Ankara University, Faculty of Veterinary Medicine. In the general clinical examination, vaginoscopy was performed and vaginal cytology was obtained from smear. The source of discharge was detected as cervix uteri. In vaginal cytologic examination, erythrocyte, neutrophile and superficial cells were observed. In abdominal ultrasonography, a hypoechoic mass was detected at cranial part of the urinary bladder. To evaluate the general health condition of the dog, total blood counting and serum biochemistry were analyzed in addition to assessing its hormone prophlye. Estrogen and progesterone levels were evaluated. Estradiol (E2) level was measured as 23 pg/mL and progesterone level was measured as 1.96 ng/mL from collected serum. The cervical mass in diameters of 3x4x2.5 cm was removed in operation. In macroscopical examination, it had spherical and regularly shape. After the macroscopical examination, tissue samples were fixed in 10% buffered formalin. Then, the samples stained with Haematoxylin-Eosin (H&E), Masson’s trichrome and PAS stainings, respectively.[...]

The evaluation of clinicopathological and immunohistochemical findings of cervical clear cell carcinoma in a bitch

Background: Clear cell carcinoma (CCC) of the cervix is a rarely seen malignant tumor which is classified in adenocarcinomas of the endometrium. It is responsible for 2.15-3.0% of endometrial tumors. The diagnosis is difficult and is still controversial in uterine carcinomas. Macroscopically, masses were protruding to endometrial surface. However, the definitive diagnosis was carried by microscopical evaluation. The cells contain clear cytoplasm. The cells are seen in this pattern because there is either glycogenation or secretoric activity in the cells.Case: In the present case, clinicopathologic and immunohistochemical findings of clear cell carcinoma of the cervix were defined in a 3 year-old spayed Golden retriever bitch. Previously spayed bitch, which had vaginal discharge for the last month-long, was submitted to the Department of Obstetrics and Gynecology, Ankara University, Faculty of Veterinary Medicine. In the general clinical examination, vaginoscopy was performed and vaginal cytology was obtained from smear. The source of discharge was detected as cervix uteri. In vaginal cytologic examination, erythrocyte, neutrophile and superficial cells were observed. In abdominal ultrasonography, a hypoechoic mass was detected at cranial part of the urinary bladder. To evaluate the general health condition of the dog, total blood counting and serum biochemistry were analyzed in addition to assessing its hormone prophlye. Estrogen and progesterone levels were evaluated. Estradiol (E2) level was measured as 23 pg/mL and progesterone level was measured as 1.96 ng/mL from collected serum. The cervical mass in diameters of 3x4x2.5 cm was removed in operation. In macroscopical examination, it had spherical and regularly shape. After the macroscopical examination, tissue samples were fixed in 10% buffered formalin. Then, the samples stained with Haematoxylin-Eosin (H&E), Massons trichrome and PAS stainings, respectively.[...](AU)

Estrogen and progesterone receptors immunolabeling in mammary solid carcinoma in an African Hedgehog (Atelerix albiventris) with concurrent uterine fibrosarcoma

The African Hedgehog (Atelerix albiventris) has gained popularity as a pet in several parts of the world. This is a report of a concomitant finding of spontaneous mammary and uterine tumors in an adult female African Hedgehog. The animal presented abdominal masses and died suddenly. Upon necropsy, not only the mammary tumor mass, but also a uterine neoplasm was found. Formalin fixed and paraffin embedded tissues were submitted for histopathological examination. The mammary tumor was diagnosed as a solid carcinoma, and the uterine tumor as fibrosarcoma. By immunohistochemistry, the mammary tumor showed positivity for pancytokeratins and estrogen and progesterone receptors, while the uterine neoplasm exhibited positivity for vimentin. There was high cross-immunoreactivity between anti-human antibodies. The positivity for hormonal receptors antigens may represent a relationship between estrogen and progesterone levels and the development of mammary tumors, as in other mammals.

Estrogen and progesterone receptors immunolabeling in mammary solid carcinoma in an African Hedgehog (Atelerix albiventris) with concurrent uterine fibrosarcoma

The African Hedgehog (Atelerix albiventris) has gained popularity as a pet in several parts of the world. This is a report of a concomitant finding of spontaneous mammary and uterine tumors in an adult female African Hedgehog. The animal presented abdominal masses and died suddenly. Upon necropsy, not only the mammary tumor mass, but also a uterine neoplasm was found. Formalin fixed and paraffin embedded tissues were submitted for histopathological examination. The mammary tumor was diagnosed as a solid carcinoma, and the uterine tumor as fibrosarcoma. By immunohistochemistry, the mammary tumor showed positivity for pancytokeratins and estrogen and progesterone receptors, while the uterine neoplasm exhibited positivity for vimentin. There was high cross-immunoreactivity between anti-human antibodies. The positivity for hormonal receptors antigens may represent a relationship between estrogen and progesterone levels and the development of mammary tumors, as in other mammals.(AU)

Uterine angioleiomyoma in an African green monkey (Chlorocebus aethiops sabaeus).

BACKGROUND: A uterine neoplasm was observed, as an incidental finding, during post-mortem examination of a 26-year-old female multiparous African green monkey (Chlorocebus aethiops sabaeus). The intramural, expansile, 2 to 3 cm well-demarcated, dark-red, nodular neoplasm was located on the anterior uterine body (corpus) wall. METHODS: The mass was examined by light microscopy and by immunohistochemistry. RESULTS: The mass was confirmed as a cavernous uterine angioleiomyoma (syn. vascular leiomyoma) characterized by abundant intratumoural vasculature lined by Factor VIII-positive endothelial cells and surrounded by smooth muscle actin-positive cell proliferations. CONCLUSION: Angioleiomyoma sharing the characteristics of intramural human cavernous uterine angioleiomyoma should be considered in the differential diagnosis of uterine tumours in non-human primates.

Adenocarcinoma endometrial em uma coelha (Oryctolagus cuniculus) / Endometrial adenocarcinoma in a rabbit (Oryctolagus cuniculus)

Background: Uterine tumors are classified according to their origin, epithelial or mesenchymal. These neoplasms are uncommon in most animal species, but are considered as important causes of reproductive disorders. In rabbits, the occurrence of these tumors increase with development of the animals and, although the numbers indicate a high occurrence of this disease in rabbits, reports are scarce. Therefore, this paper has the objective to describe a case of endometrial adenocarcinoma in a rabbit, emphasizing their histological features as well as provide a brief discussion about the epidemiological, clinical, pathological and prevention forms and treatment. Case: A 4-years old New Zealand rabbit breed, from a group of animals maintained only for breeding, had low fertility. The rabbit was twice covered by proven fertile animals, but no pregnancy, becoming unfit reproduction and subjected to euthanasia. At necropsy, tissue samples were collected, subsequently fixed in 10% formalin and then were processed routinely and stained with hematoxylin and eosin for histopathological analysis. Grossly, there was swelling of the uterine body, characterized by two masses that protruded from the endometrium, with rounded and soft look, and the court had cystic cavity filled with whitish gelatinous consistency of content. Microscopic cancer cells that originated from the endometrial...

Adenocarcinoma endometrial em uma coelha (Oryctolagus cuniculus) / Endometrial adenocarcinoma in a rabbit (Oryctolagus cuniculus)

Background: Uterine tumors are classified according to their origin, epithelial or mesenchymal. These neoplasms are uncommon in most animal species, but are considered as important causes of reproductive disorders. In rabbits, the occurrence of these tumors increase with development of the animals and, although the numbers indicate a high occurrence of this disease in rabbits, reports are scarce. Therefore, this paper has the objective to describe a case of endometrial adenocarcinoma in a rabbit, emphasizing their histological features as well as provide a brief discussion about the epidemiological, clinical, pathological and prevention forms and treatment. Case: A 4-years old New Zealand rabbit breed, from a group of animals maintained only for breeding, had low fertility. The rabbit was twice covered by proven fertile animals, but no pregnancy, becoming unfit reproduction and subjected to euthanasia. At necropsy, tissue samples were collected, subsequently fixed in 10% formalin and then were processed routinely and stained with hematoxylin and eosin for histopathological analysis. Grossly, there was swelling of the uterine body, characterized by two masses that protruded from the endometrium, with rounded and soft look, and the court had cystic cavity filled with whitish gelatinous consistency of content. Microscopic cancer cells that originated from the endometrial...(AU)

Tumor uterino em bovino – relato de caso / Tumor - case report

As descrições de tumores uterinos em bovinos não são freqüentes, embora o câncer uterino não seja um achado raro. Este relato apresenta o caso de um bovino do sexo feminino, raça Holandesa, 11 anos de idade, escore de condição corporal 3,5, oito partos e produção leiteira de sete litros à época do atendimento, que foi atendido a campo em propriedade rural de atividade leiteira. À palpação retal, foi diagnosticada uma massa tumoral de consistência firme no corpo do útero, estendendo-se até um dos cornos. Ao exame ultrassonográfico, foi confirmada a presença de tumoração no corpo uterino com 15,3 cm de diâmetro. O tratamento cirúrgico foi descartado pelo proprietário, devido aos riscos cirúrgicos e dificuldade com os cuidados pós-operatórios a campo. Anestro e ciclos estrais curtos, com 10 a 15 dias de duração, foram os principais sinais clínicos observados.

Descriptions of uterine tumors in cattle are infrequent, although uterine cancer is not a rare finding. This report presents the case of a bovine female, Holstein, 11 years of age, body condition score 3.5, eight births and seven liters of milk production at the time of the service, which was attended in rural property of dairy activity. During transrectal palpation, was diagnosed a tumor with firm mass in the body of the uterus, extending to one of the horns. Ultrasound exam confirmed the presence of the tumor in the uterine body with 15.3 cm diameter. Surgical treatment was discarded by the owner due to surgical risks and difficulty with postsurgical care at field. Anestrus and short estrous cycles, with 10-15 days of duration, were the main clinical signs.

Tumor uterino em bovino relato de caso / Tumor - case report

As descrições de tumores uterinos em bovinos não são freqüentes, embora o câncer uterino não seja um achado raro. Este relato apresenta o caso de um bovino do sexo feminino, raça Holandesa, 11 anos de idade, escore de condição corporal 3,5, oito partos e produção leiteira de sete litros à época do atendimento, que foi atendido a campo em propriedade rural de atividade leiteira. À palpação retal, foi diagnosticada uma massa tumoral de consistência firme no corpo do útero, estendendo-se até um dos cornos. Ao exame ultrassonográfico, foi confirmada a presença de tumoração no corpo uterino com 15,3 cm de diâmetro. O tratamento cirúrgico foi descartado pelo proprietário, devido aos riscos cirúrgicos e dificuldade com os cuidados pós-operatórios a campo. Anestro e ciclos estrais curtos, com 10 a 15 dias de duração, foram os principais sinais clínicos observados. (AU)

Descriptions of uterine tumors in cattle are infrequent, although uterine cancer is not a rare finding. This report presents the case of a bovine female, Holstein, 11 years of age, body condition score 3.5, eight births and seven liters of milk production at the time of the service, which was attended in rural property of dairy activity. During transrectal palpation, was diagnosed a tumor with firm mass in the body of the uterus, extending to one of the horns. Ultrasound exam confirmed the presence of the tumor in the uterine body with 15.3 cm diameter. Surgical treatment was discarded by the owner due to surgical risks and difficulty with postsurgical care at field. Anestrus and short estrous cycles, with 10-15 days of duration, were the main clinical signs. (AU)

Neoplasias en aparato reproductor en perras: estudio restrospectivo a 6 años / Neoplasias in the reproductive system in female dogs: retrospective study of 6 years

Las noeplasias en tracto genital son poco frecuentes en perras, y suelen estar asociadas a problemas reproductivos secundarios. El objetivo del presente estudio fue analizar la frecuencia de neoplasias en el aparato reproductor de perras en México, haciendo una revisión retrospectiva de biopsias remitidas al Departamento de Patología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional Autónoma de México, desde 1988 hasta 1993. Con ese fin se revisaron 2062 biopsias, de las cuales 117 (5.6 por ciento) correspondieron a neoplasias en el aparato reproductor. Se localizaron 48 neoplasias en vagina (41.02 por ciento), 40 en vulva (34.18 por ciento), 18 en ovario (15.38 por ciento) y 11 en útero (9.4 por ciento). De acuerdo con el tipo histológico, las neoplasias más frecuentes fueron: 8 casos de tumor de células de la granulosa (44.4 por ciento) en ovario, 5 casos de adenoma (45.5 por ciento) en útero y 31 (64.6 por ciento) y 22 (55 por ciento) casos de tumor venéreo transmisible en vagina y vulva, respectivamente. Las edades de los animales fueron de más de 5 años. Los datos encontrados concuerdan con lo referido en la literatura