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1.
Sci Total Environ ; 914: 169874, 2024 Mar 01.
Article in English | MEDLINE | ID: mdl-38185174

ABSTRACT

Human blood has been commonly and routinely analyzed to determine internal human exposure to parabens. However, data on the occurrence of parabens and their common metabolite, p-hydroxybenzoic acid (4-HB), in different human blood matrixes is still limited. In this study, 139 pairs of serum and whole blood samples were collected from Chinese adults, and then analyzed them for 5 parabens and 4-HB. Methylparaben (MeP) and propylparaben (PrP) were consistently the predominant parabens in human serum (mean 2.3 and 2.1 ng/mL, respectively) and whole blood (1.9 and 1.3 ng/mL, respectively). Mean concentrations of 4-HB in human serum and whole blood were 7.7 and 12 ng/mL, respectively. Concentrations of parabens, except benzylparaben (BzP), and 4-HB in human serum were significantly (p < 0.01) correlated with that in whole blood. Distribution pattern of parabens and 4-HB in human blood was evaluated, for the first time, based on their partitioning between human serum and whole blood (Kp). Mean Kp values of parabens, except BzP, increased with the alkyl chain length from 0.83 to 1.6. BzP (mean 1.4) had a comparable mean Kp value to PrP (mean 1.4). Among target analytes, 4-HB had the lowest mean Kp value (0.75). These data are important to select appropriate blood matrixes for conducting human exposure assessment and epidemiological studies on parabens.


Subject(s)
Blood , Parabens , Adult , Humans , Parabens/pharmacokinetics
2.
Appl Opt ; 62(36): 9462-9469, 2023 Dec 20.
Article in English | MEDLINE | ID: mdl-38108770

ABSTRACT

A design of a photonic crystal nanocavity based bio-sensor having a footprint of 12×8µm 2 is proposed to detect different blood components. A finite difference time domain (FDTD) numerical technique has been used to characterize the sensor by evaluating its frequency response. The shift in resonant wavelength of the proposed cavity is utilized to detect blood refractive index fluctuation due to the presence of various components. The obtained numerical findings show that the maximum sensitivity for a shift in resonant wavelength is reported as 760 nm/RIU for various blood components. Moreover, the fabrication of PhC is always prone to the fabrication induced disorders. Hence, the impact of fabrication imperfections on the sensor's performance also has been included in the analysis.


Subject(s)
Biosensing Techniques , Blood Chemical Analysis , Photons , Blood
3.
Ann Parasitol ; 69: 0, 2023 11 26.
Article in English | MEDLINE | ID: mdl-38007222

ABSTRACT

In the experiment, 160 medicinal leeches of the species Hirudo verbana Carena, 1820 were studied. Medicinal leeches were fed on the blood of animals and people (conditionally healthy and diseased). Four leeches were taken from each animal/person. The animals were studied for 3 weeks. Mortality was mostly observed in the first days after feeding on the blood of the host. We noted mortality, the appearance of constrictions on the leeches' body, the intensity of the host blood spitting from their body. The host's blood was taken from their stomach on the first day after feeding. Hematological and immunological indicators of blood were determined in the taken blood of the host. As a result of the study of the blood of the sick, significant changes were found, compared to conditionally healthy ones. It was manifested by an increase in erythrocytes and leukocytes. The leukocyte formula looked like in most pathological conditions of the inflammatory process. The obtained indicators of the experiment make it possible to quickly assess the presence of physiological disorders in the early stages of the disease.


Subject(s)
Leeches , Animals , Humans , Blood , Erythrocytes/pathology , Leukocytes/pathology
4.
San Salvador; MINSAL; sept. 04, 2023. 70 p. ilus, graf.
Non-conventional in Spanish | BISSAL, LILACS | ID: biblio-1510000

ABSTRACT

El presente manual de procesos y procedimientos documenta los servicios que se ofrecen en la atención al usuario interno y externo para la gestión de la sangre, inmunohematología y hemoterapia como parte del proceso de atención en salud integral e integrada a la persona en el curso de vida con enfoque de atención primaria en salud, describe el sistema de operación ofrecido en los establecimientos de salud, mediante el enfoque por procesos, fomentando así el desarrollo organizacional y el mejoramiento continuo para el cumplimiento de la misión institucional. Establece las bases para la ejecución de los procedimientos como parte de los procesos institucionales, unificando criterios de contenido que permite la sistematización de las actividades y la definición de la metodología para efectuarlas. Esta herramienta táctica y operativa, permite integrar las actividades y tareas de manera oportuna, para el logro de la prestación de servicios con calidad en los establecimientos de salud que lo necesiten, facilitando el cumplimiento de las normativas y lineamientos de programas especiales o por ciclo de vida vigentes en el Ministerio de Salud, así como la armonización con la sistematización y uso de herramientas tecnológicas que sea necesario implementar para volver más eficaz el trabajo del talento humano en salud


This manual of processes and procedures documents the services offered in the care of internal and external users for blood management, immunohematology and hemotherapy as part of the process of comprehensive and integrated health care for the person throughout the life course. With a focus on primary health care, it describes the operation system offered in health establishments, through the process approach, thus promoting organizational development and continuous improvement to fulfill the institutional mission. Establishes the bases for the execution of procedures as part of institutional processes, unifying content criteria that allows the systematization of activities and the definition of the methodology to carry them out. This tactical and operational tool allows the integration of activities and tasks in a timely manner, to achieve the provision of quality services in the health establishments that need it, facilitating compliance with the regulations and guidelines of special programs or by cycle of life in force in the Ministry of Health, as well as harmonization with the systematization and use of technological tools that need to be implemented to make the work of human talent in health more effective


Subject(s)
Blood , Blood Group Antigens , Hemotherapy Service , Manuals as Topic , El Salvador
5.
Science ; 381(6661): 932-933, 2023 09.
Article in English | MEDLINE | ID: mdl-37651522

ABSTRACT

Proposal could allow synthetic proteins to replace harvested enzyme in drug testing.


Subject(s)
Blood Coagulation Factors , Conservation of Natural Resources , Horseshoe Crabs , Limulus Test , Animals , Blood
6.
Washington, D.C.; OPS; 2023-05-01.
Non-conventional in Spanish | PAHO-IRIS | ID: phr-57421

ABSTRACT

La finalidad de un sistema nacional de sangre es garantizar que todos los pacientes que necesitan una transfusión tengan un acceso oportuno a suministros de sangre y componentes sanguíneos de calidad, seguros y suficientes. Sin embargo, numerosos países aún no pueden proporcionar suministros suficientes de sangre entera y de componentes sanguíneos a los pacientes en todo momento o en todas las ubicaciones geográficas. Esta orientación ofrece a los responsables de la toma de decisiones, especialmente en los países de ingresos medianos y bajos, un marco para sopesar la posibilidad de adoptar un enfoque centralizado del análisis y el procesamiento de las donaciones de sangre en centros de transfusión designados. Se explican las etapas clave de la planificación de un centro y se ofrece información práctica sobre la introducción de sistemas de calidad, gestión de riesgos y gestión de la información, y sobre la gestión de los recursos humanos, económicos y de infraestructura necesarios para que el centro lleve a cabo el análisis y el procesamiento de las donaciones de sangre de manera centralizada.


Subject(s)
Blood , Blood Banks , Blood Donation , Blood Transfusion , Blood Component Transfusion , Health Systems
7.
Environ Res ; 228: 115928, 2023 07 01.
Article in English | MEDLINE | ID: mdl-37076032

ABSTRACT

Heavy metals and plastic pollutants are the two most disastrous challenges to the environment requiring immediate actions. In this work, a techno-commercially feasible approach to address both challenges is presented, where a waste polypropylene (PP) based reversible sensor is produced to selectively detect copper ions (Cu2+) in blood and water from different sources. The waste PP-based sensor was fabricated in the form of an emulsion-templated porous scaffold decorated with benzothiazolinium spiropyran (BTS), which produced a reddish colour upon exposure to Cu2+. The presence of Cu2+ was checked by naked eye, UV-Vis spectroscopy, and DC (Direct Current) probe station by measuring the current where the sensor's performance remained unaffected while analysing blood, water from different sources, and acidic or basic environment. The sensor exhibited 1.3 ppm as the limit of detection value in agreement with the WHO recommendations. The reversible nature of the sensor was determined by cyclic exposure of the sensor towards visible light turning it from coloured to colourless within 5 min and regenerated the sensor for the subsequent analysis. The reversibility of the sensor through exchange between Cu2+- Cu+ was confirmed by XPS analysis. A resettable and multi-readout INHIBIT logic gate was proposed for the sensor using Cu2+ and visible light as the inputs and colour change, reflectance band and current as the output. The cost-effective sensor enabled rapid detection of the presence of Cu2+ in both water and complex biological samples such as blood. While the approach developed in this study provides a unique opportunity to address the environmental burden of plastic waste management, it also allows for the possible valorization of plastics for use in enormous value-added applications.


Subject(s)
Blood , Copper , Metals, Heavy , Polypropylenes , Copper/chemistry , Ions/analysis , Water
8.
Malar J ; 22(1): 115, 2023 Apr 07.
Article in English | MEDLINE | ID: mdl-37029433

ABSTRACT

BACKGROUND: Control of malaria parasite transmission can be enhanced by understanding which human demographic groups serve as the infectious reservoirs. Because vector biting can be heterogeneous, some infected individuals may contribute more to human-to-mosquito transmission than others. Infection prevalence peaks in school-age children, but it is not known how often they are fed upon. Genotypic profiling of human blood permits identification of individual humans who were bitten. The present investigation used this method to estimate which human demographic groups were most responsible for transmitting malaria parasites to Anopheles mosquitoes. It was hypothesized that school-age children contribute more than other demographic groups to human-to-mosquito malaria transmission. METHODS: In a region of moderate-to-high malaria incidence in southeastern Malawi, randomly selected households were surveyed to collect human demographic information and blood samples. Blood-fed, female Anopheles mosquitoes were sampled indoors from the same houses. Genomic DNA from human blood samples and mosquito blood meals of human origin was genotyped using 24 microsatellite loci. The resultant genotypes were matched to identify which individual humans were sources of blood meals. In addition, Plasmodium falciparum DNA in mosquito abdomens was detected with polymerase chain reaction. The combined results were used to identify which humans were most frequently bitten, and the P. falciparum infection prevalence in mosquitoes that resulted from these blood meals. RESULTS: Anopheles females selected human hosts non-randomly and fed on more than one human in 9% of the blood meals. Few humans contributed most of the blood meals to the Anopheles vector population. Children ≤ 5 years old were under-represented in mosquito blood meals while older males (31-75 years old) were over-represented. However, the largest number of malaria-infected blood meals was from school age children (6-15 years old). CONCLUSIONS: The results support the hypothesis that humans aged 6-15 years are the most important demographic group contributing to the transmission of P. falciparum to the Anopheles mosquito vectors. This conclusion suggests that malaria control and prevention programmes should enhance efforts targeting school-age children and males.


Subject(s)
Anopheles , Blood , Host-Seeking Behavior , Malaria, Falciparum , Adolescent , Adult , Aged , Animals , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Anopheles/parasitology , DNA/blood , Genotype , Malaria/blood , Malaria/parasitology , Malaria/prevention & control , Malaria/transmission , Malaria, Falciparum/blood , Malaria, Falciparum/parasitology , Malaria, Falciparum/prevention & control , Malaria, Falciparum/transmission , Meals , Mosquito Vectors/parasitology , Plasmodium falciparum/genetics , Blood/parasitology , Malawi
9.
Langmuir ; 39(13): 4712-4719, 2023 04 04.
Article in English | MEDLINE | ID: mdl-36944107

ABSTRACT

This study aims to understand and compare the evaporation dynamics of drops of healthy and pathological porcine blood (glomerulonephritis disease) evaporated on hydrophilic glass substrates at different surface temperatures (Ts): 23, 37, 60, and 90 °C. Subsequently, the different induced phenomena are characterized and described. Additionally, drops of water were evaporated at these four surface temperatures to better understand the difference between healthy and pathological porcine blood. Statistical studies were performed to analyze the evaporation rate, the maximum and average values of Marangoni numbers (Ma), and the evaporated specific time. The statistical tests showed significant differences in these parameters between healthy and pathological blood for each surface temperature. The mean and the maximum of the Ma increase with the increase in Ts caused by the increase in the temperature differences between the edge and the center of the drop. When comparing healthy and diseased blood, the Ma maximum and mean of healthy blood were higher than those of diseased blood for all Ts. Besides, this study emphasizes the influence of temperature on blood evaporation and the pattern caused by the Marangoni effect. These results demonstrate that differences between the two blood types are related to the disease and pave the way to developing a new methodology for medical decision-making.


Subject(s)
Water , Animals , Hydrophobic and Hydrophilic Interactions , Swine , Temperature , Water/chemistry , Blood
10.
J Virol ; 97(4): e0167022, 2023 04 27.
Article in English | MEDLINE | ID: mdl-36971588

ABSTRACT

Elucidating the mechanisms underlying the persistence and location of the HIV reservoir is critical for developing cure interventions. While it has been shown that levels of T-cell activation and the size of the HIV reservoir are greater in rectal tissue and lymph nodes (LN) than in blood, the relative contributions of T-cell subsets to this anatomic difference are unknown. We measured and compared HIV-1 DNA content, expression of the T-cell activation markers CD38 and HLA-DR, and expression of the exhaustion markers programmed cell death protein 1 (PD-1) and T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domains (TIGIT) in naive, central memory (CM), transitional memory (TM), and effector memory (EM) CD4+ and CD8+ T-cells in paired blood and LN samples among 14 people with HIV who were receiving antiretroviral therapy. HIV-1 DNA levels, T-cell immune activation, and TIGIT expression were higher in LN than in blood, especially in CM and TM CD4+ T-cell subsets. Immune activation was significantly higher in all CD8+ T-cell subsets, and memory CD8+ T-cell subsets from LN had higher levels of PD-1 expression, compared with blood, while TIGIT expression levels were significantly lower in TM CD8+ T-cells. The differences seen in CM and TM CD4+ T-cell subsets were more pronounced among participants with CD4+ T-cell counts of <500 cells/µL within 2 years after antiretroviral therapy initiation, thus highlighting increased residual dysregulation in LN as a distinguishing feature of and a potential mechanism for individuals with suboptimal CD4+ T-cell recovery during antiretroviral therapy. IMPORTANCE This study provides new insights into the contributions of different CD4+ and CD8+ T-cell subsets to the anatomic differences between LN and blood in individuals with HIV who have optimal versus suboptimal CD4+ T-cell recovery. To our knowledge, this is the first study comparing paired LN and blood CD4+ and CD8+ T-cell differentiation subsets, as well as those subsets in immunological responders versus immunological suboptimal responders.


Subject(s)
CD4-Positive T-Lymphocytes , CD8-Positive T-Lymphocytes , DNA, Viral , HIV Infections , Lymph Nodes , Lymphocyte Activation , Humans , Lymph Nodes/cytology , Lymph Nodes/immunology , Lymph Nodes/virology , DNA, Viral/analysis , HIV-1 , HIV Infections/drug therapy , HIV Infections/virology , Blood/immunology , Blood/virology , Lymphocyte Activation/immunology , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/virology , CD8-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/virology , Male , Adult , Middle Aged , Programmed Cell Death 1 Receptor/genetics , Programmed Cell Death 1 Receptor/metabolism , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/virology
11.
Transl Psychiatry ; 13(1): 72, 2023 02 27.
Article in English | MEDLINE | ID: mdl-36843037

ABSTRACT

Neuroepigenetics considers genetic sequences and the interplay with environmental influences to elucidate vulnerability risk for various neurological and psychiatric disorders. However, evaluating DNA methylation of brain tissue is challenging owing to the issue of tissue specificity. Consequently, peripheral surrogate tissues were used, resulting in limited progress compared with other epigenetic studies, such as cancer research. Therefore, we developed databases to establish correlations between the brain and peripheral tissues in the same individuals. Four tissues, resected brain tissue, blood, saliva, and buccal mucosa (buccal), were collected from 19 patients (aged 13-73 years) who underwent neurosurgery. Moreover, their genome-wide DNA methylation was assessed using the Infinium HumanMethylationEPIC BeadChip arrays to determine the cross-tissue correlation of each combination. These correlation analyses were conducted with all methylation sites and with variable CpGs, and with when these were adjusted for cellular proportions. For the averaged data for each CpG across individuals, the saliva-brain correlation (r = 0.90) was higher than that for blood-brain (r = 0.87) and buccal-brain (r = 0.88) comparisons. Among individual CpGs, blood had the highest proportion of CpGs correlated to the brain at nominally significant levels (19.0%), followed by saliva (14.4%) and buccal (9.8%). These results were similar to the previous IMAGE-CpG results; however, cross-database correlations of the correlation coefficients revealed a relatively low (brain vs. blood: r = 0.27, saliva: r = 0.18, and buccal: r = 0.24). To the best of our knowledge, this is the fifth study in the literature initiating the development of databases for correlations between the brain and peripheral tissues in the same individuals. We present the first database developed from an Asian population, specifically Japanese samples (AMAZE-CpG), which would contribute to interpreting individual epigenetic study results from various Asian populations.


Subject(s)
DNA Methylation , Humans , Brain , CpG Islands , DNA , East Asian People , Epigenesis, Genetic , Epithelium , Saliva , Adolescent , Young Adult , Adult , Middle Aged , Aged , Blood , Cheek
12.
PLoS One ; 18(2): e0281594, 2023.
Article in English | MEDLINE | ID: mdl-36791130

ABSTRACT

High-throughput omics technologies have enabled the profiling of entire biological systems. For the biological interpretation of such omics data, two analyses, hypothesis- and data-driven analyses including tensor decomposition, have been used. Both analyses have their own advantages and disadvantages and are mutually complementary; however, a direct comparison of these two analyses for omics data is poorly examined.We applied tensor decomposition (TD) to a dataset representing changes in the concentrations of 562 blood molecules at 14 time points in 20 healthy human subjects after ingestion of 75 g oral glucose. We characterized each molecule by individual dependence (constant or variable) and time dependence (later peak or early peak). Three of the four features extracted by TD were characterized by our previous hypothesis-driven study, indicating that TD can extract some of the same features obtained by hypothesis-driven analysis in a non-biased manner. In contrast to the years taken for our previous hypothesis-driven analysis, the data-driven analysis in this study took days, indicating that TD can extract biological features in a non-biased manner without the time-consuming process of hypothesis generation.


Subject(s)
Blood , Metabolome , Humans , Blood Chemical Analysis
13.
Int J Mol Sci ; 24(4)2023 Feb 11.
Article in English | MEDLINE | ID: mdl-36835073

ABSTRACT

There is still a need for an efficient method for the isolation of extracellular vesicles (EVs) from human blood that provides a reliable yield with acceptable purity. Blood is a source of circulating EVs, but soluble proteins and lipoproteins hamper their concentration, isolation, and detection. This study aims to investigate the efficiency of EV isolation and characterization methods not defined as "gold standard". EVs were isolated from human platelet-free plasma (PFP) of patients and healthy donors through size-exclusion chromatography (SEC) combined with ultrafiltration (UF). Then, EVs were characterized using transmission electron microscopy (TEM), imaging flow cytometry (IFC), and nanoparticle tracking analysis (NTA). TEM images showed intact and roundish nanoparticles in pure samples. IFC analysis detected a prevalence of CD63+ EVs compared to CD9+, CD81+, and CD11c+ EVs. NTA confirmed the presence of small EVs with a concentration of ~1010 EVs/mL that were comparable when stratifying the subjects by baseline demographics; conversely, concentration differed according to the health status across healthy donors and patients affected with autoimmune diseases (130 subjects in total, with 65 healthy donors and 65 idiopathic inflammatory myopathy (IIM) patients). Altogether, our data show that a combined EV isolation method, i.e., SEC followed by UF, is a reliable approach to isolate intact EVs with a significant yield from complex fluids, which might characterize disease conditions early.


Subject(s)
Chromatography, Gel , Extracellular Vesicles , Ultrafiltration , Humans , Chromatography, Gel/methods , Extracellular Vesicles/chemistry , Lipoproteins/metabolism , Microscopy, Electron, Transmission , Ultrafiltration/methods , Blood
14.
Vox Sang ; 118(2): 164, 2023 02.
Article in English | MEDLINE | ID: mdl-36541571
17.
Rev. enferm. atenção saúde ; 12(3): 202396, jul.-out. 2023. ilus
Article in English, Spanish, Portuguese | BDENF - Nursing | ID: biblio-1444800

ABSTRACT

Objetivo: identificar os cuidados para segurança do paciente no ato transfusional. Método: revisão integrativa da literatura, realizada em abril de 2020, nas fontes de dados LILACS, BDENF, Scielo, Pubmed e Scopus. Resultados: os cuidados foram categorizados em três etapas: pré-transfusão, transfusão e pós-transfusão. Na etapa pré-transfusional, identificou-se os cuidados relacionados a correta identificação do paciente; orientação do receptor quanto ao procedimento; avaliação do acesso venoso; verificação dos sinais vitais; e, utilização de equipo próprio para hemocomponentes. Na etapa da transfusão, destacaram-se os cuidados para monitoramento do receptor; identificação de sinais e sintomas de reações transfusionais; e, controle do tempo máximo de infusão. Na etapa pós-transfusão, evidencia-se a verificação de sinais vitais e o registro do procedimento no prontuário do paciente. Conclusão: evidenciou-se que identificar os cuidados no ato transfusional é fundamental para promoção de práticas seguras nesse processo, com vistas à eliminação de falhas evitáveis (AU).


Objective: to identify the care for patient safety during the transfusion. Methods: integrative literature review, carried out in April 2020, in the LILACS, BDENF, Scielo, Pubmed and Scopus databases. Results: the care were rated in three stages: pre-transfusion, transfusion and post-transfusion. In the pre-transfusion stage, it was identified care related to the correct identification of the patient; receiver's orientation about the procedure; assessment of venous access; checking of vital signs; and, use of the intravenous administration set for blood components. In the transfusion stage, it was highlighted care to monitor the recipient; identification of signs and symptoms of transfusion reaction; and control of the maximum infusion time. In the posttransfusion stage, it was evidenced the checking vital signs and recording the procedure in the patient's medical record. Conclusion: it became evident that identify care in the transfusion act is fundamental to promote safe practices in this process, with a view to eliminating preventable failures (AU).


Objetivo: identificar los cuidados para la seguridad del paciente durante la transfusion. Metodo: revisión integrativa de la literatura, realizada en abril de 2020, en las bases de datos LILACS, BDENF, Scielo, Pubmed y Scopus. Resultados: los cuidados se categorizó en tres etapas: pretransfusión, transfusión y postransfusión. En la etapa pretransfusion, se identificó los cuidados relacionados con la correcta identificación del paciente; orientación del receptor con respecto al procedimiento; evaluación del acceso venoso; verificación de los signos vitales; y uso de equipos para componentes sanguíneos. En la etapa de transfusion, se tuvo cuidado de monitorear al receptor, identificación de signos y síntomas de reacciones transfusionales y controlar el tiempo máximo de infusión. En la etapa postransfusión, se evidencia la verificación de los signos vitales y el registro del procedimiento en la historia clínica del paciente. Conclusión: Se hizo evidente que identificar el cuidado en el acto transfusional es fundamental para promover prácticas seguras en este proceso, con miras a eliminar fallas evitables (AU).


Subject(s)
Blood , Blood Transfusion , Nursing , Blood Safety , Patient Safety
18.
Ann. afr. méd. (En ligne) ; 16(2): 5046-5057, 2023. tables
Article in French | AIM (Africa) | ID: biblio-1425733

ABSTRACT

Contexte & objectif. Les modifications lipidiques chez les enfants obèses en Afrique noire sont peu documentées. Les objectifs de la présente étude étaient de déterminer chez les adolescents obèses le profil lipidique, et analyser les associations entre quelques paramètres anthropométriques et lipidiques. Méthodes. L'étude transversale a été réalisée à Brazzaville auprès de 82 adolescents âgés de 11 à 18 ans, répartis en 45 sujets obèses, 17 en état de surpoids et 20 poids normal. Des mesures de la taille, du poids, du tour de taille, des plis cutanés sous-scapulaire et tricipital ont été effectuées. Des prélèvements sanguins ont permis de déterminer les concentrations en cholestérol total, cholestérol-LDL, cholestérol-HDL et triglycérides. Résultats. Les concentrations lipidiques notées chez les adolescents obèses étaient significativement supérieures à celles des sujets de poids normal : cholestérol total, 1,70 vs 1,59g/L ; cholestérol-LDL, 1,03 vs 0,88g/L ; triglycérides, 1,18 vs 0,86. Par contre, celles du cholestérol-HDL étaient significativement inférieures : 0,42 vs 0,51g/L. Une corrélation positive a été retrouvée entre le rapport tour de taille/taille et le cholestérol-HDL (r=0,75 ; p=0,031). Conclusion. Nos résultats soulignent la nécessité de renforcer la prise en charge des enfants obèses afin de prévenir les facteurs de risque potentiels des maladies cardiovasculaires à l'âge adulte


Subject(s)
Humans , Cross-Sectional Studies , Adolescent Health , Heart Disease Risk Factors , Blood , Pediatric Obesity
19.
Arch. latinoam. nutr ; 72(4): 285-293, dic. 2022. ilus, tab
Article in Spanish | LILACS, LIVECS | ID: biblio-1413581

ABSTRACT

Introducción: La alimentación en la primera infancia influencia la instauración del tejido adiposo y el desarrollo de diversas patologías en la edad adulta. Objetivo: Evaluar la influencia del consumo de tres fuentes de ácidos grasos sobre parámetros sanguíneos y tejido adiposo en pollos recién eclosionados. Materiales y métodos: Se utilizaron 76 pollitos Cobb 500 distribuidos aleatoriamente en cuatro tratamientos, que fueron alimentados durante siete días con una de las cuatro dietas (T1: 97% Dieta basal (DB); T2: DB +3% de manteca vegetal parcialmente hidrogenada; T3: DB +3% de aceite de quinua y T4: DB +3% de aceite de pescado). Al finalizar, se evaluó en sangre glucosa, colesterol, triglicéridos y tamaño de adipocitos del tejido adiposo subcutáneo y visceral. Se aplicó ANOVA considerando 0,05 de significancia y en el caso de variables no distribuidas normalmente, se aplicó el test no paramétrico de Kruskal-Wallis mediante el programa R-Studio. Resultados: Se obtuvieron diferencias significativas con disminución de los niveles de glucosa y colesterol en animales suplementados con elevada proporción de aceites insaturados (T3 y T4) en comparación a T2. Los tratamientos T3 y T4 promovieron una formación hiperplásica de adipocitos, diferenciándose significativamente de T2, que promovió la hipertrofia en dichas células, esta respuesta fue similar en ambos depósitos subcutáneos. Conclusiones: El consumo de aceite de quinua y aceite de pescado promueve la formación de tejido adiposo saludable, y reducen los niveles de glucosa y colesterol. Contrariamente el consumo de manteca vegetal propicia la hipertrofia de adipocitos de gran tamaño e incrementa los parámetros bioquímicos evaluados(AU)


Introduction: Feeding in early childhood influences the establishment of adipose tissue, and therefore also in the development of various pathologies in adulthood. Objective: To evaluate the influence of the consumption of three sources of fatty acids on blood parameters and adipose tissue at an early age. Materials and methods: 76 Cobb 500 chicks randomly distributed in four treatments were used, who were fed for seven days with one of the four diets (T1: Basal diet; T2: DB +1.0% vegetable shortening partially hydrogenated; T3: DB +1.0% quinoa oil and DB +1.0% fish oil) until the seventh day of life. At the end, glucose, cholesterol, triglycerides and adipocyte size of the subcutaneous and visceral adipose tissue were evaluated. A completely randomized design with ANOVA considering 0.05 significance was applied and in the case of non-normally distributed variables, the non-parametric Kruskal-Wallis test was applied using the R-Studio program. Results: Significant differences were obtained with a decrease in glucose and cholesterol levels in animals supplemented with unsaturated oils (T3 and T4) compared to T2. Regarding the size of adipocytes, treatments T3 and T4 promoted a hyperplastic formation of adipocytes, differing significantly from T2, which promoted hypertrophy in these cells, this response was similar in both subcutaneous deposits. Conclusions: The consumption of quinoa oil and fish oil promote the formation of healthy adipose tissue, in addition to reducing glucose and cholesterol levels. In contrast, the consumption of vegetable shortening favors the hypertrophy of large adipocytes and increases the biochemical parameters evaluated(AU)


Subject(s)
Animals , Blood , Chickens , Adipogenesis , Fatty Acids , Vegetables , Fish Oils , Adipose Tissue , Cholesterol , Chenopodium quinoa , Intra-Abdominal Fat
20.
Metas enferm ; 25(9): 0676-0677, Nov. 2022. ilus
Article in Spanish | IBECS | ID: ibc-213271

ABSTRACT

José Manuel Martín González trabaja en la consulta de acccesos venosos del Hospital Virgen del Rocío (Sevilla), un servicio que nació en 2017 y desde entonces atienden a un 80% más de pacientes. En un primer momento estaba pensada para pacientes oncológicos y hematológicos, pero se ha ampliado y en ella se atienden a todos aquellos que puedan presentar problemas o dificultades a la hora de instaurarle una vía para la administración de fármacos, o bien precisen terapia intravenosa de larga duración.(AU)


Subject(s)
Humans , Male , Nurses, Male , Nursing , Nursing Services , Blood , Blood Chemical Analysis
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