1H NMR-based approach to determine the geographical origin and cultivation method of roasted coffee.

A comprehensive study of 603 roasted arabica coffee samples using NMR fingerprinting and multivariate data analysis was performed to differentiate coffee samples according to their geographical origin and cultivation method. Both lipophilic and hydrophilic coffee metabolites were recorded using 1H NMR spectroscopy, and principal component analysis followed by linear discriminant analysis (PCA-LDA) was applied. Coffee samples were fist differentiated according to their continents of origin followed by discrimination of coffee samples from Brazil, Ethiopia, and Colombia from coffee samples originating from another continent. Discrimination of coffee samples according to their continent of origin and additional assignment to the countries Brazil and Ethiopia were successful. However, an unambiguous separation of Colombian coffee samples from coffee samples of another continent (other than South America) was not possible. Also, differentiation of organically and conventionally produced coffee samples by using 1H NMR and PCA-LDA was not achieved.

A green method for anthocyanin extraction from Clitoria ternatea flowers cultivated in southern Brazil: Characterization, in vivo toxicity, and biological activity.

This study aimed to develop a green method to obtain an anthocyanin-rich edible extract of Clitoria ternatea flowers grown in southern Brazil. The extract was characterized by UHPLC-MSn and analyzed for toxicological potential in an in vivo model, total phenolic content, and biological activities. By using a 23 multivariate design to study the effects of temperature, acidified solvent, and time on the total anthocyanin content (487.25 mg/g), total phenolic content (2242.47 mgGAE/g), it was possible to determine the optimal point (45 °C, 16 min, and 22.5 mL extraction solution). Thirteen anthocyanins and nine non-anthocyanins were quantified. In vivo toxicity assay using Galleria mellonella showed a safe concentration when administered up to 2.2 g of extract per body kg. The extract showed antioxidant activity and antibacterial action against food pathogens, the method proved to have a low environmental impact, in addition to producing an extract with potential for application in food.

Landscape changes in the Cerrado: Challenges of land clearing, fragmentation and land tenure for biological conservation.

Land clearing, low levels of protection, and high biodiversity make the Brazilian Cerrado a hotspot for biological conservation. However, one of the most active agricultural frontiers in Brazil is located in this region. We thus aimed to evaluate the current trends of deforestation and fragmentation of the Cerrado, from 1986 to 2019; and the contribution of land tenure and farm size to the Cerrado conservation. We divided the Cerrado (∼2 Mkm2) into three sub-regions and calculated the distribution and size of the fragments; core areas and edge distance; isolation and importance of the smallest fragments for reducing isolation; and connectivity for the years 1986, 1997, 2008, and 2019. We then evaluated vegetation cover and landscape metrics for public lands and private farms. Since 1986, 22 % of the Cerrado's remnant vegetation was cleared and the number of fragments increased by 20 %. Currently, 10 % of the Cerrado vegetation is under the effect of a 30 m edge. Isolation increased in all the sub-regions and smaller fragments (>100 ha) are important for landscape configuration. 10.82 % of the vegetation is preserved in public lands and 57.9 % in private farms, where 377,901.5 km2 could be legally cleared. Compared to other Brazilian regions, the northern Cerrado is relatively well connected and less fragmented but land clearing still threatens biodiversity. Public lands are important for connectivity and habitat amount but play a minor role when compared to private lands. Our results highlight that avoiding further land clearing of the Cerrado is a challenge that requires the engagement of different stakeholders at different levels.

Cannabis sativa L. roots from Northeast Brazil reduce abdominal contortions in a mouse model of primary dysmenorrhea.

ETHNOPHARMACOLOGICAL RELEVANCE: Although the root of Cannabis sativa L. has been mentioned in some regions, such as the Vale do São Francisco, for its potential traditional medicinal use as an anti-inflammatory, anti-asthmatic, and against gastrointestinal diseases, it has received little exploration and discussion. AIM OF THE STUDY: This study aimed to perform a chemical analysis of an aqueous extract of Cannabis sativa roots (AqECsR) and evaluate its pharmacological effects against uterine disorders, in vivo and ex vivo, in rodents. MATERIALS AND METHODS: The roots were provided by the Brazilian Federal Police, and the freeze-dried extract was used for the chemical analysis of the AqECsR by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The sample was subsequently used in three doses for pharmacological assays (12.5, 25, and 50 mg/kg), which included the spasmolytic activity test and the primary dysmenorrhea test. The primary dysmenorrhea test aimed to verify the effect of AqECsR on induced abdominal contortions in female mice in vivo and to perform a morphometric analysis of the organs. Association tests at subtherapeutic doses of AqECsR with antidysmenorrheic drugs were also performed. RESULTS: The data obtained by HPLC-MS suggested the presence of four substances: cannabisativine, anhydrocannabisativine, feruloyltyramine, and p-coumaroyltyramine. In the pharmacological assays, the AqECsR showed no spasmolytic effect. However, in the antidysmenorrheal activity test, AqECsR demonstrated a significant in vivo effect of reducing oxytocin-induced abdominal contortions. Morphometric analysis of the uterus showed no significant organ enlargement effect, and the association of AqECsR with subtherapeutic doses of three drugs used in antidysmenorrheal therapy (mefenamic acid, scopolamine, and nifedipine) showed an effect in reducing abdominal contortions. CONCLUSIONS: In conclusion, AqECsR contains four chemical compounds and exhibits an antidysmenorrheic effect both alone and in association with drugs, reducing abdominal contortions in female mice without generating organ enlargement in the animals. Further studies are needed to prove the mechanism of action by which AqECsR promotes its effect on primary dysmenorrhea and to explore its associations.

The traditional use of native Brazilian plants for male sexual dysfunction: Evidence from ethnomedicinal applications, animal models, and possible mechanisms of action.

ETHNOPHARMACOLOGICAL RELEVANCE: Sexual dysfunction is a multifactorial health condition characterized by distressing disturbances in the sexual response and libido, leading to an inability to maintain penile erection and achieve pleasure. Considering the huge Brazilian biodiversity, many plants are traditionally used for aphrodisiac purposes. However, the use of native medicinal plants as sexual boosters in Brazil has been poorly studied. AIM OF THE STUDY: This review focuses on the composition, pharmacology, and results of experimental trials of the main native plants used in Brazilian folk medicine with alleged aphrodisiac effects. We aimed to provide a state-of-the-art reference for research on herbs for the treatment of male sexual dysfunction by summarizing and discussing the main studies found. MATERIALS AND METHODS: The relevant information was collected by searching keywords (aphrodisiac, sexual tonic, sexual stimulant, sexual vigor stimulant, sexual impotency, erectile dysfunction, etc.) from books containing primary surveys conducted in the original communities and bibliographic surveys prepared by authors linked to the national academic and scientific environment edited in Brazil. Preclinical and clinical studies of the compiled plant species were performed using scientific databases (Scopus, PubMed, SciELO, and SciFinder). RESULTS: Seventy-four plant species belonging to 44 families used in Brazil to treat sexual dysfunction were compiled from ethnopharmacological literature. Fourteen plants, including Pfaffia glomerata (Spreng.) Pedersen, Aspidosperma quebracho-blanco Schltdl., Anemopaegma arvense (Vell.) Stellfeld ex de Souza, Mimosa pudica L., Heteropterys tomentosa A. Juss., Trichilia catigua A. Juss., and Turnera diffusa Willd. ex Schult. were pharmacologically studied to confirm these therapeutic properties. Probable modes of action include antioxidant and androgenic activities, inhibition of the PDE5 enzyme, increase in NO levels, and activation of dopaminergic and noradrenergic pathways. In addition, several different species popularly known as "catuaba" were identified, leading to adulterations and controversial effects. CONCLUSION: The overall results of the present review of Brazilian folk literature reveal that Brazil has a long tradition of using plants with potential aphrodisiac effects. However, further research is required to identify, characterize, and standardize the active ingredients and herbal preparations used in aphrodisiacs.

A short climatology of black and brown carbon and their sources at a suburban site impacted by smoke in Brazil.

Emissions from biomass burning challenge efforts to curb air pollution in cities downwind of fire-prone regions, as they contribute large amounts of brown carbon (BrC) and black carbon (BC) particles. We investigated the patterns of BrC and BC concentrations using Aethalometer data (at λ = 370 and 880 nm, respectively) spanning four years at a site impacted by the outflow of smoke. The data required to be post processed for the shadowing effect since, without correction, concentrations would be between 29% and 35% underestimated. The BrC concentrations were consistently higher than the BC concentrations, indicating the prevalence of aerosols from biomass burning. The results were supported by the Ångström coefficient (Å370/880), with values predominantly larger than 1 (mean ± standard deviation: 1.25 ± 0.31). Å370/880 values below 1 were more prevalent during the wet season, which suggests a contribution from fossil fuel combustion. We observed sharp BrC and BC seasonal signals, with mean minimum concentrations of 0.40 µg/m3 and 0.36 µg/m3, respectively, in the wet season, and mean maximum concentrations of 2.05 µg/m3 and 1.53 µg/m3 in the dry season. The largest concentrations were observed when northerly air masses moved over regions with a high density of fire spots. Local burning of residential solid waste and industrial combustion caused extreme BrC and BC concentrations under favourable wind directions. Although neither pollutant is included in any ambient air quality standards, our results suggest that transboundary smoke may hamper efforts to meet the World Health Organization guidelines for fine particles.

Copper isotopes as a tool to trace contamination in mangroves from an urbanized watershed.

This study investigates the chronology of copper (Cu) contamination and its stable isotopes within an emblematic Brazilian mangrove impacted by multiple urban and industrial Cu sources, deforestation, and eutrophication. In particular, it tests Cu isotopes as tracers of anthropogenic inputs into an anthropized watershed impacted by multiple sources. To do so, we used multi-isotopic approaches (δ65Cu, δ13C, and δ15N), elemental analyses (Al, Ca, Fe, P, Cu, C, and N), and selective and sequential extractions in a210Pb-dated sediment core. This geochemical "toolbox" allowed identifying two main stages of Cu evolution in the sediment core. In the first stage, before 1965, Cu isotope fingerprints responded to landscape changes, indicating a shift from marine to geogenic dominance due to the remobilization and erosion of terrestrial materials. In the second stage, after 1965, the sediment geochemical profile showed increased Cu total concentrations with a higher bioavailability (as reflected by sequential extraction data) accompanying changes in Cu isotope signatures towards anthropogenic values. The findings evidence that local industrial sources, possibly combined with diffuse urban sources, export Cu into downstream mangroves with a distinguishable isotope signature compared to natural values. This study demonstrates the applicability of Cu isotopes as new environmental forensic tools to trace anthropogenic sources in mangrove sediments. Incorporated into a robust geochemical toolbox that combines inorganic and organic proxies for sedimentary materials, this new tool provides a comprehensive understanding of Cu dynamics in mangrove ecosystems, shedding light on the historical and current sources of Cu.

Short-term air pollution exposure and mortality in Brazil: Investigating the susceptible population groups.

This is the first study to examine the association between ambient air pollution (PM2.5, O3, and NO2) and mortality (in different population groups by sex and age) based on a nationwide death record across Brazil over a 15-year period (2003-2017). We used a time-series analytic approach with a distributed lag model. Our study population includes 2,872,084 records of deaths in Brazil between 2003 and 2017. Men accounted for a higher proportion of deaths, with 58% for all-cause mortality, 54% for respiratory mortality, and 52% for circulatory mortality. Most individuals were over 65 years of age. Our results suggest an association between air pollution and mortality in Brazil. The direction, statistical significance, and effect size of these associations varied considerably by type of air pollutant, region, and population group (sex and age group). In particular, the older population group (>65 years) was most affected. The national meta-analysis for the entire data set (without stratification by sex and age) showed that for every 10 µg/m3 increase in PM2.5 concentration, the risk of death from respiratory diseases increased by 2.93% (95%CI: 1.42; 4.43). For every 10 ppb increase in O3, there is a 2.21% (95%CI: 0.59; 3.83) increase in the risk of all-cause mortality for the group of all people between 46 and 65 years old, and a 3.53% (95%CI: 0.34; 6.72) increase in the risk of circulatory mortality for the group of women, all ages. For every 10 ppb increase in NO2, the risk of respiratory mortality increases by 17.56% (95%CI: 4.44; 30.64) and the risk of all-cause mortality by 5.63% (95%CI: 1.83; 9.44). The results of our study provide epidemiological evidence that air pollution is associated with a higher risk of cardiorespiratory mortality in Brazil. Given the lack of nationwide studies on air pollution in Brazil, our research is an important contribution to the local and international literature that can provide better support to policymakers to improve air quality and public health.

Predictive Performance for Hospital Mortality of SAPS 3, SOFA, ISS, and New ISS in Critically Ill Trauma Patients: A Validation Cohort Study.

Background: It is not known whether anatomical scores perform better than general critical care scores for trauma patients admitted to the intensive care unit (ICU). We compare the predictive performance for hospital mortality of general critical care scores (SAPS 3 and SOFA) with anatomical injury-based scores (Injury Severity Score [ISS] and New ISS [NISS]). Methods: Retrospective cohort study of patients admitted to a specialized trauma ICU from a tertiary hospital in São Paulo, Brazil between May, 2012 and January, 2016. We retrieved data from the ICU database for critical care scores and calculated ISS and NISS from chart data and whole body computed tomography results. We compared the predictive performance for hospital mortality of each model through discrimination, calibration, and decision-curve analysis. Results: The sample comprised 1053 victims of trauma admitted to the ICU, with 84.2% male patients and mean age of 40 (±18) years. Main injury mechanism was blunt trauma (90.7%). Traumatic brain injury was present in 67.8% of patients; 43.3% with severe TBI. At the time of ICU admission, 846 patients (80.3%) were on mechanical ventilation and 644 (64.3%) on vasoactive drugs. Hospital mortality was 23.8% (251). Median SAPS 3 was 41; median maximum SOFA within 24 h of admission, 7; ISS, 29; and NISS, 41. AUROCs (95% CI) were: SAPS 3 = 0.786 (0.756-0.817), SOFA = 0.807 (0.778-0.837), ISS = 0.616 (0.577-0.656), and NISS = 0.689 (0.649-0.729). In pairwise comparisons, SAPS 3 and SOFA did not differ, while both outperformed the anatomical scores (p < .001). Maximum SOFA within 24 h of admission presented the best calibration and net benefit in decision-curve analysis. Conclusions: Trauma-specific anatomical scores have fair performance in critically ill trauma patients and are outperformed by SAPS 3 and SOFA. Illness severity is best characterized by organ dysfunction and physiological variables than anatomical injuries.

Climate risks and vulnerabilities of the Arabica coffee in Brazil under current and future climates considering new CMIP6 models.

The susceptibility to climate change concerns the coffee market worldwide due to possible severe productivity losses. Brazil is the world's largest Arabica coffee producer and has crops in regions considered persistent climate change hotspots. Our study analyzed risks, vulnerabilities, and susceptibilities to pests and diseases in these regions under current and future climates and outlined adaptive measures to reduce future vulnerabilities. Ten risk indicators based on Arabica coffee requirements were proposed: water supply (Iw), base (TIB) and maximum temperature stresses (TImax), which delimit the temperature range where Arabica coffee grows and productivity is penalized outside both ranges, frost stress (TIfrost), diseases such as rust (DIrust), brown eye spot (DIbrown), and Phoma leaf spot (DIphoma), pests such as coffee berry borer (PIberry), coffee leaf miner (PIminer), and yield loss due to water stress (Iyg). Daily near-surface air temperature (minimum, mean, and maximum), relative humidity, precipitation, and global solar radiation were used from 16 General Circulation Models (GCMs) from the NASA Earth Exchange Global Daily Downscaled Projections (NEX-GDDP), which are derived from the Coupled Model Intercomparison Project Phase 6 (CMIP6) in three Shared Socioeconomic Pathways scenarios (SSP245, SSP370 and SSP585). All risk indicators were calculated for the current climate (1995-2014) and projected for the near (2041-2060), intermediate (2061-2080), and far future (2081-2100) in three SSPs and then classified into five risk classes (very low, low, moderate, high and very high). Our results indicated that due to increases in TImax and Iyg indicators, with high to very high risk in area and magnitude, Arabica coffee plantations will be negatively affected and economically unfeasible for about 35 % to 75 % of the studied area throughout the 21st century. Furthermore, the rust and the leaf miner will remain a concern in future climates due to increased temperatures and reduced relative humidity. The future of Arabica coffee crops in Brazil will depend on adopting effective adaptive measures and prudent agricultural strategies to address anticipated risks, including shifting crops to higher altitude areas, introducing more climate-resilient coffee cultivars/varieties, using agroforestry or intercropping systems, planting in closer spacing or higher density planting, and employing dripper or partial root-zone irrigation techniques.

Women in Surgical Residency Programs in Brazil: A Trend Analysis.

INTRODUCTION: There is a gender gap in the surgical field worldwide. Brazil and low- and middle-income countries generally tend to have a more profound gap. Therefore, we aim to assess the gender distribution in Brazilian surgical residencies. METHODS: From a national residencies' database, we collected residency entering years, names, and surgical subspecialties. We classified gender from the names using Gender API software and performed linear regression, binomial, and chi-square tests. RESULTS: From 81,979 doctors (1931-2020), 36.6% were women (P < 0.001). Of 13 subspecialties, regarding the absolute numbers of women, only neurosurgery and plastic surgery did not significantly differ between gender per year. There was a statistically significant growth in the proportional rates of female representation among most of the surgical residencies analyzed. Overall, the least women's representation was in urology (3.8%). From all subspecialties analyzed, both genders had a significant linear absolute increase over the years (P < 0.05), except for obstetrics-gynecology and pediatric surgery for men. Until 1990, all surgical programs had at least one woman resident, except for colorectal surgery. CONCLUSIONS: Although there is an overall lack of women surgeons in Brazil, we observed an increase in women in surgical residencies. Therefore, the study may demonstrate a future change in the Brazilian gender equity scenario and reflect the growing representation of women in medicine in Brazil, except for some surgical specialties.

Cross-Cultural Adaptation, Reliability, and Convergent Validity of Occupational Self-Assessment for Brazilian Portuguese.

The Occupational Self-Assessment version 2.2 (OSA) is a self-assessment of the client's occupational competence and values. To describe the process of cross-cultural adaptation of OSA into Brazilian Portuguese (OSA-Brazil) and examine its reliability and validity for use with the Brazilian population. Assessment translation was guided by two international guidelines for cross-cultural adaptation of standardized instruments. Face validity was tested with 24 participants. Internal consistency, test-retest reliability, and convergent validity were tested with a convenience sample of 40 participants. The cross-cultural adaptation process concluded with a consensus among the expert panel review (r > 80%) and evidence of strong face validity. The OSA-Brazil demonstrated appropriate test-retest reliability (r > 0.70) and convergent validity with the 36-Item Short-Form Health Survey (SF-36) (p < .05). The OSA-Brazil has good face validity, test-retest reliability, and convergent validity. The assessment can be used by the Brazilian occupational therapists to assess client's occupational competence.

Comparison of molecular techniques for the detection of Toxoplasma gondii in raw bovine milk from small rural properties in Brazil.

This study aimed to research Toxoplasma gondii DNA in 102 samples of raw bovine milk from expansion tanks, in small properties located in different cities of the Midwest region of São Paulo, Brazil. For this, polymerase chain reaction (PCR) was performed with the primers TOX4/TOX5 for cPCR (conventional PCR), TgNP1/TgNP2 gene for nested PCR and the Tg18s58F/Tg18s348R for nested PCR. It was possible to detect T. gondii DNA in 18 (17.65 %) milk samples from the 102 tanks, corresponding to 4.90 % for TOX4/TOX5 primers, 12.74 % for TgNP1/TgNP2 gene and 0.98 % for Tg18s58F/Tg18s348R gene. The results showed that the TgNP1 and TgNP2 genes were more efficient to detect T. gondii DNA, and also indicated the importance of raw bovine milk as a source of human infections caused by this protozoan, being a public health problem. It is important to continue studies involving T. gondii from bovine milk considering the need for proper pasteurization, and for better comprehension regarding the epidemiology of this protozoan.

Metal levels in waste pickers in Brasilia, Brazil: hair and nail as exposure matrices.

The aim of this cross-sectional study was to compare workplace conditions and metal exposures in 431 waste pickers who worked nearby at the Estrutural Dump in Brasilia utilizing hair (n = 310) and nail (n = 355) as matrices of exposure. Waste pickers were grouped according to their workplace (open waste dump: G1 and sorting plants: G2). Hair and nail samples were collected and analyzed using ICP-MS. The work duration in the facilities was significantly different between the groups with averages of 16.46 (8.48) yrs and 9.26 (6.28) yrs for hair donors in G1 and G2, and 15.92 (7.72) yrs and 8.55 (5.77) yrs for toenail donors in G1 and G2, respectively. The arithmetic means (µg/g) of cadmium, copper, lead, and manganese in hair were significantly higher in G2 (0.076 ± 0.133; 19.61 ± 18.16; 2.27 ± .56 and 3.87 ± 5.59, respectively) compared to G1 (0.069 ± 0.235; 15.72 ± 15.18; 1.72 ± 4.04 and 3.65 ± 5.5, respectively). Concentrations of arsenic, barium, cadmium, copper, cobalt, lead, manganese, and molybdenum in nail were significantly higher in G2 (0.57 ± 0.39; 22.74 ± 42.06; 0.1 ± 0.08; 22.7 ± 51.60; 0.48 ± 0.45; 4.69 ± 9.43; 19.07 ± 20.75; 1.80 ± 1.76, respectively) compared to G1 (0.40 ± 0.28; 15.32 ± 22.31; 0.08 ± 0.11; 11.91 ± 16.25; 0.37 ± 0.37; 3.94 ± 15.04; 13.01 ± 19.08; 1.16 ± 1.80, respective. Our findings suggest that the studied population was exposed to toxic metals and indicates the need for chemical exposure prevention policies to monitor chemical risk exposures in waste pickers.

Determination of pesticide residue in marginal lagoons of natural parks in Brazil using an improved calibrate passive sampler.

Passive sampling is a sensitive and efficient method for analyzing pesticides in water. This article describes a hollow fiber liquid-phase microextraction (HF-LPME) device that was improved using polypropylene membranes grafted with nanocellulose for the passive sampling of pesticides in water. A comprehensive gas chromatography time-of-flight mass spectrometry (GCxGC/Q-TOFMS) system was used to separate, identify, and quantify pesticides. The sampling rates of 38 moderately hydrophobic to hydrophobic agricultural pesticides (2.18 < log Kow < 6.89) from different chemical classes, including the main triazine, organochlorine and organophosphate compounds, were calculated. A calibration process was applied to evaluate the role of flow velocity and select potential candidates for a possible performance reference compound (PRC). Sampling rates varied between 0.17 mL d-1 and 23.15 mL d-1. The accumulation curves identified linear periods ranging from 3 to 18 days. The new passive sampler device was applied for 8 days in rivers and marginal lagoons of natural parks of the São Francisco basin in Minas Gerais, Brazil and identified 10 target pesticides. Furthermore, 10 non-targeted pesticides were detected by the GCxGC/Q-TOFMS method.

Evaluation of exposure to multiple organic pollutants in riparian communities of the Brazilian Amazon: Screening levels and potential health risks.

Organic pollutants are widely distributed in the environment. Due to their physical and chemical characteristics, they tend to be biomagnified in food chains, mainly in aquatic organisms. Therefore, food consumption is a significant route of lifelong exposure. Although the Amazon River basin contains the highest freshwater biodiversity on Earth, there is scarce literature focusing on the levels of organic pollutants in the local population. The present study was aimed at assessing the levels of several environmental pollutants (polycyclic aromatic hydrocarbons, bisphenols, parabens, and benzophenones) in urine samples from riverside communities along the Tapajós and Amazon Rivers in the Brazilian Amazon region. The results show a 100 % detection of naphthalene metabolites (namely, 1-hydroxy-naphthalene (1OH-NAP), 2-hydroxy-naphthalene (2OH-NAP)), with the highest levels belonging to benzylparaben (BzP) (17.3 ng/mL). Gender-specific analysis revealed that women had significantly higher levels of certain PAH metabolites (i.e., 1OH-NAP and 2-hydroxy-fluorene (2OH-FLU)) than men. In turn, most of the evaluated compounds were higher in urine samples from people living near the Amazon River, which presents increased traffic of boats and ships than the Tapajós River. On the other hand, the human health risk assessment suggested that all communities are at risk of suffering non-carcinogenic effects from exposure to PAHs. At the same time, they are also at risk of carcinogenic effects from exposure to benzo[a]pyrene metabolites. Thus, further studies are needed in order to evaluate the potential health effects of exposure to a mixture of these organic pollutants and other contaminants present in the area, such as mercury.

Local and landscape factors influencing mercury distribution in water, bottom sediment, and biota from lakes of the Araguaia River floodplain, Central Brazil.

Mercury (Hg) is a chemical element widely present in the Earth's crust. However, its high toxicity and ability to accumulate in organisms and biomagnify through food chains characterize it as a global pollutant of primary control. We assessed total mercury concentrations ([THg]) in abiotic and biotic compartments from 98 floodplain lakes associated with the Araguaia River and six tributaries (Midwest Brazil). [THg] quantification in water was performed by cold vapor atomic fluorescence spectroscopy. [THg] in bottom sediment was assessed using cold vapor generation atomic absorption spectrophotometry, while [THg] in macrophyte, periphyton, and plankton were quantified by thermal decomposition atomic absorption spectrometry. Hotspots of [THg] in water, bottom sediment, and macrophytes were determined in areas impacted by pasture and urban areas. In contrast, hotspots of [THg] in periphyton and forest fires were determined in preserved areas downstream. [THg] in plankton did not show a clear spatial distribution pattern. The mean bioaccumulation factor order was plankton (2.3 ± 1.8) > periphyton (1.3 ± 0.9) > macrophytes (0.7 ± 0.4) (KW = 55.09, p < 0.0001). Higher [THg] in water and bottom sediment were associated with high pH (R2adj = 0.118, p = 0.004) and organic matter (R2adj = 0.244, p < 0.0001). [THg] in macrophytes were positively influenced by [THg] in water (R2adj = 0.063, p = 0.024) and sediment (R2adj = 0.105, p = 0.007). [THg] in periphyton are positively related to forest fires (R2adj = 0.156, p = 0.009) and [THg] in macrophytes (R2adj = 0.061, p = 0.03) and negatively related to lake depth (R2adj = 0.045, p = 0.02). The transfer of Hg from water and sediment to the biota is limited. However, the progressive increase of the bioaccumulation factor between macrophyte, periphyton, and plankton may indicate Hg biomagnification along the food chain of the Araguaia River floodplain.

Soil δ15N spatial distribution is primarily shaped by climatic patterns in the semiarid Caatinga, Northeast Brazil.

Soil nitrogen isotopic composition (δ15Nsoil) is an invaluable tool as it integrates nitrogen (N) transformations in soils. In addition to serving as a baseline to understand the N cycle, spatial representations of δ15Nsoil across landscapes (or isoscapes) is a multi-purpose tool useful to investigate, for example, plant-microbe interactions, animal migration and forensics. We investigate the climatic and edaphic controls of δ15Nsoil utilising data from 29 geographical locations sampled across the semiarid Brazilian Caatinga biome. The sampling covered a mean annual precipitation (PA) gradient ranging from 0.51 to 1.36 m a-1 and eight soil types originating from three different geological origins. Our data show that the combination of higher aridity and lower seasonality (ψ) leads to higher values of δ15Nsoil. Moreover, soil total carbon had a positive relationship with δ15Nsoil, appearing within the best-supported models according to the information-theoretic approach undertaken here. The contribution to the plant communities by the Fabaceae trees expressed as their basal area was not related to δ15Nsoil values, suggesting that the magnitude of biological N fixation in the Caatinga is not large enough to be reflected in the soil. In addition, considering PA in a categorical fashion, i.e., 'high' (> 0.8 m a-1) and 'low' PA (< 0.8 m a-1), we found that, within the wetter category, δ15Nsoil was positively related to several soil properties (i.e., clay content, effective cation exchange capacity, exchangeable calcium, silt content, pHH2O, total phosphorus and sum of bases) and negatively related to sand content. Our study provides new insights into the functioning of semiarid ecosystems from a pedo-isotopic perspective and contributes to the overall understanding of the N cycle in the Caatinga region, with the potential to support the development of new conceptualisation of biogeochemical process and testing of global models that simulate N and C cycles.

Incomplete sanitation in the Metropolitan Region of São Paulo results in detection of SARS-CoV-2 in headwater streams.

Among the largest metropolitan regions in the world, the Metropolitan Region of São Paulo (MRSP) represents an important case study for the COVID-19 respiratory disease pandemic because it is home to >20 million people, making it one of the largest metropolitan regions in the global south. Besides the high population density, the MRSP has several problems related to social and economic aspects, which may reflect in the dynamics of SARS-CoV-2 virus, such as low income, lack of sanitation and social vulnerability in the peripheral regions of MRSP. In these regions, the input of untreated sewage on the streams and rivers can be frequently observed, which may represent an indicator of poor sewer system. Therefore, this study aimed to identify if streams draining urbanized regions without appropriate sanitation are prone to receive higher loads of detectable SARS-CoV-2 in its waters. For this, we collected water samples from 45 headwater streams distributed across an urbanization gradient (0-100 % of urbanization) in the MRSP, with three replicates in each stream and analyzed the concentrations of SARS-CoV-2 RNA targeting the nucleocapsid N1 and N2 genomic regions. In addition, we analyzed the relationship between the concentrations of SARS-CoV-2 RNA and sanitation and social variables. Our results showed that the concentrations of SARS-CoV-2 RNA were higher in the streams draining medium to high urbanized catchments, especially because of the lack of sanitation and the higher probabilities to detect SARS-CoV-2 RNA in the stream water was associated with households without a septic tank or sewage system within the catchment, followed by per capita household income. These results reflect the lack of urbanization planning and the lack of sanitation, especially in the poor regions from the MRSP, adding another risk for the already vulnerable population in a metropolitan region from the global south during a pandemic disease.

Meso- and microplastic composition, distribution patterns and drivers: A snapshot of plastic pollution on Brazilian beaches.

Pollution by plastics is a worldwide problem on par with climatic change and biological invasions. In coastal sediments, plastic particles tend to accumulate and persist over the long term. We assessed the plastic pollution using a standardized surface sediment sampling protocol on 22 sandy beaches along >4600 km of the Brazilian coast. The abundance, size, color, type, and polymeric composition of all meso- and microplastic items found in the surveys were processed to disclose spatial patterns of distribution and pollution associated drivers. A General Linear Model (GLM) was run to investigate how the predictor variables influenced overall beach plastic amounts and by plastic type and size class. Overall, 3114 plastic items were found, with microplastics comprising just over half of all items (54 %). Most items were either white (60 %) or blue (13 %), while polystyrene foam (45 %) and fragments (39 %) comprised the most abundant plastic types. The principal polymers were Polyethylene (40 %) and Polypropylene (32 %). The analyses indicated that the distribution of plastic litter along beaches is determined by three predictive variables: the distance to the nearest estuary (-), tourism (+), and the number of inhabitants in the nearest urban center (+). Tourist (highly-visited) beaches and those near estuarine runoffs or urban centers presented the highest plastic pollution rates. The unveiling of plastic pollution patterns through a large-scale systematic survey is essential for future management guidance and science-based decisions for mitigating and solving the plastic pollution crisis.