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1.
São Paulo; AMHB; mar. 3, 2020. 21 p.
Monography in Portuguese | LILACS, HomeoIndex Homeopathy, MOSAICO - Integrative health | ID: biblio-1087382

ABSTRACT

Estamos no meio da pandemia do Covid-19, em franco desenvolvimento no Brasil. No momento há perspectivas terapêuticas medicamentosa sem fases iniciais de teste, especialmente para o tratamento dos casos graves, aqueles que implicam em internação ou encaminhamento para Unidades de Terapia Intensiva. Pouco tem sido abordado sobre tentativas de tratamento de pacientes com síndrome respiratória leve. Não há escolha terapêutica efetiva para o início do quadro e, desta forma, a doença cursa de acordo com a resposta imune ou suscetibilidade individual do acometido, e há muito pouco de efetivo e específico que altere a história natural dessa enfermidade. (AU)


Subject(s)
Arsenicum Album/therapeutic use , China officinalis/therapeutic use , Chininum Arsenicosum/therapeutic use , Epidemic Genius , Coronaviridae , Coronaviridae Infections , Bryonia , Pandemics , Homeopathy , Brazil/epidemiology
2.
Mol Med Rep ; 20(1): 73-80, 2019 Jul.
Article in English | MEDLINE | ID: mdl-31115513

ABSTRACT

Bryonia dioica Jacq. is a climbing perennial herb with tuberous roots which is widely used in traditional medicine in Algeria for the treatment of cancer. The present study aimed to evaluate the apoptogenic activity and phytochemical composition of the aqueous extract of B. dioica roots growing in Algeria. The cytotoxic effect of B. dioica aqueous decoction against breast cancer MDA­MB­231 cells was evaluated by an MTT assay. Apoptosis induction was assessed by an Annexin V­fluorescien iosthiocyanate assay. Propidium iodide staining of cell DNA was used to assess the effects on the cell cycle. In addition to UV­Visible (UV­vis) analysis, the major compounds of the extracts were determined using liquid chromatography­mass spectrometric analyses. Our results showed that the B. dioica aqueous extract induced cell death in a time­dependent manner. The highest inhibitory effect was produced at concentrations of 50 µg/ml or higher after 72 h of treatment (91.15±0.71%). Furthermore, the extract induced apoptosis of MDA­MB­231 cells. At 250 µg/ml, 64.61% of the treated MDA­MB­231 cells were apoptotic. This was accompanied by cell cycle arrest at G2/M phase. The percentage of cells in G2/M increased from 15.7% (untreated cells) to 59.13% (50 µg/ml) and 58.51% (250 µg/ml). The UV­vis absorption spectra of B. dioica aqueous extract showed two absorption bands characteristic of the flavonol skeleton; 350­385 nm (Band I) and 250­280 nm (Band II). Myricetin (2,5,7,3,4,5­pentahydroxylflavonol) was found to be the major compound in the B. dioica aqueous extract. These findings suggest that B. dioica could be considered a promising source for developing novel therapeutics against breast cancer.


Subject(s)
Breast Neoplasms/drug therapy , Bryonia/chemistry , Cell Proliferation/drug effects , Plant Extracts/pharmacology , Apoptosis/drug effects , Breast Neoplasms/pathology , Cell Cycle Checkpoints/drug effects , Cell Line, Tumor , Female , G2 Phase Cell Cycle Checkpoints , Humans , Plant Extracts/chemistry
3.
J Ethnobiol Ethnomed ; 15(1): 22, 2019 May 09.
Article in English | MEDLINE | ID: mdl-31072383

ABSTRACT

INTRODUCTION: White bryony, Bryonia alba L., is a relatively little known plant in the history of folk medicine and folk botany in eastern and northern Europe. The main aim of this article is to bring together data about Bryonia alba and to summarise its cultural history and folk botanical importance in eastern and northern Europe. Nowadays, this species is considered at best as an ornamental plant, and at worst as a noxious weed. However, ethnographic and historical sources show that it used to be of magical, medicinal and ritual importance in our part of Europe. METHODS: A diachronic perspective was chosen in order to outline and analyse the devolution and changes in the use of B. alba, in the course of which we take into account the social, ecological and chemical aspects of the usage of this plant. We have therefore traced down and analysed published sources such as ethnographical descriptions, floras, linguistic records and topographical descriptions from northern and central-eastern Europe, particularly Scandinavia, Baltic States, Germany, Poland, Belarus, Ukraine and the Balkan Peninsula. The analysed material is presented and discussed within the biocultural domains that developed in the interaction between human societies and Bryonia alba. RESULTS AND DISCUSSION: Bryonia alba has many folk names in northern and central-eastern parts of Europe: some of them refer to its medicinal properties, life form, odour, or toxicity; others to its possession by the devil. As we learn, Bryonia alba was an inexpensive surrogate for mandrake (Mandragora officinarum L.) and sold as such in the discussed parts of Europe. The folklore and medicinal properties ascribed to mandrake were passed on to white bryony due to an apparent resemblance of the roots. In ethnographic descriptions, we find a mixture of booklore, i.e. written traditions, and oral traditions concerning this species. Some of this folklore must have been an alternative stories spread by swindlers who wished to sell fake mandrake roots to people. CONCLUSIONS: Plant monographs and reviews of particular species tend to concentrate on the botanicals, which might have great useful potential. White bryony presents a precisely opposite example, being a plant that used to be of medicinal relevance and was furnished with symbolical meaning, and has nowadays preserved only its ornamental value among some urban and rural dwellers of northern Europe. Nonetheless, it might be considered as a part of the biocultural heritage in old, well-preserved gardens. It is still used as a medicine in some parts of the Balkan Peninsula.


Subject(s)
Bryonia , Medicine, Traditional , Phytotherapy , Plants, Medicinal , Europe , Humans
4.
J Pharm Pharmacol ; 71(2): 230-239, 2019 Feb.
Article in English | MEDLINE | ID: mdl-30324727

ABSTRACT

OBJECTIVES: The aim of the present study consisted in the isolation of flavonoids from the leaves of Bryonia alba L. and evaluation of their antioxidant activity and inhibition on peroxidase-catalysed reactions. METHODS: Flavonoids were isolated by preparative HPLC-DAD and their structures were elucidated by MS and NMR. Inhibitory effect was tested by the horseradish peroxidase and the myeloperoxidase assays. Cellular antioxidant assays consisted in testing the inhibitory activity on the reactive oxygen species released upon activation of neutrophils freshly isolated ex vivo from equine blood and of human monocytes-derived macrophages in vitro. Whole organism toxicity was assessed on zebrafish larvae. KEY FINDINGS: Four flavonoids (lutonarin, saponarin, isoorientin and isovitexin) were isolated. The performed assays showed significant antioxidant activity and inhibition for the peroxidase-catalysed reactions. Absence of cellular and zebrafish toxicity was confirmed. CONCLUSIONS: Bryonia alba L. leaves are particularly interesting for their flavonoids content and showed significant inhibitory effect on peroxidase-catalysed oxidation of substrates (Amplex Red and L012), as well as antioxidant/antiradical activity, proving that this species has a medicinal potential. Moreover, the present study highlights the absence of the toxicity of these leaves and offers though a novel perspective on the species, previously known as being toxic.


Subject(s)
Antioxidants/pharmacology , Bryonia/chemistry , Flavonoids/pharmacology , Plant Extracts/pharmacology , Animals , Antioxidants/chemistry , Antioxidants/isolation & purification , Chromatography, High Pressure Liquid/methods , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/isolation & purification , Enzyme Inhibitors/pharmacology , Flavonoids/chemistry , Flavonoids/isolation & purification , HL-60 Cells , Horses , Humans , Macrophages/drug effects , Monocytes/cytology , Peroxidase/antagonists & inhibitors , Plant Extracts/chemistry , Plant Leaves , Reactive Oxygen Species/metabolism , Zebrafish
5.
Int. j. high dilution res ; 18(3/4): 35-46, 2019.
Article in English | LILACS, HomeoIndex Homeopathy | ID: biblio-1050038

ABSTRACT

Background ­ Diabetes Mellitus a metabolic disorder affects the secretion of insulin from pancreas leading to hyperglycemia, if uncontrolled leads to complications triggered by free radical formed after oxidative stress. Homeopathic medicine Cephalandra Indica has shown antidiabetic activity in various potencies performed on preclinical studies on diabetic rat model. The present review highlights the pharmacological profile of homeopathic preparations Cephalandra Indica on preclinical studies and calculating the probable human equivalent dosage from preclinical studies for the pilot studies. Method ­ Articles published between January 1988 and December 2018 was included in review. Databases like PubMed ­ Medline, Google scholar were used for collecting the articles. Keywords like 'Homeopathy' or 'Homoeopathy', 'Invitro', 'Invivo' and 'Cephalandra Indica' were used. SABEH criteria were implemented for assessing methodology quality of articles. Results ­ Seven full text articles were included in review which had six Invivo studies and one Invitro study. This review article provided the scientific validation of high diluted homeopathic medicines pharmacological activity of Cephalandra Indica and probable mechanism of action confirmed through preclinical studies. Conversion of dosage from animal model to human dosage for pilot studies has been hypothetically proposed. Conclusion ­ Homeopathic medicine Cephalandra Indica has a therapeutic and safety profile with no toxicity observed in preclinical studies. The proposed hypothesis of conversion of dosage needs to be validated for further studies. (au)


Subject(s)
Diabetes Mellitus , Drug Evaluation, Preclinical , Homeopathy , Bryonia
6.
Int. j. high dilution res ; 18(3/4): 47-62, 2019.
Article in English | LILACS, HomeoIndex Homeopathy | ID: biblio-1050039

ABSTRACT

Background Simple and mucopurulent chronic bronchitis (SMCB) is characterized by recurrent mucoid or mucopurulent expectoration in absence of localized suppurative disease. This observational open label study was undertaken to evaluate the effects of homeopathic medicine in SMCB. Methods 1902 patients were screened from 07 centres out of which 1305 were excluded. 597 patients were enrolled as per the inclusion and exclusion criteria. A total of 14 pre-defined homeopathic medicines were shortlisted for prescription after repertorizing the pathological symptoms of SMCB. Outcomes were assessed through chronic bronchitis symptom scale (CBSS) and FEV1/ FVC ratio with spirometry for over a period of two years. Appearance of any change (relief/ worse)/ status quo was immediately followed by placebo/ change in dilution/ change in remedy. Statistical analysis was done using SPSS version 20. Results: 532 patients were analyzed based on the intention to treat principle using last observation carry forward method. Mean CBS score reduced from 29.86±4.5 at baseline to 12.33±7.6 at completion of 2 years. Repeated measures ANOVA, at time points 0 (baseline), 3, 6, 9, 12 ,15, 18, 21 and 24 months, showed significant reduction in CBS scores [Wilk's Lambda 0.104, F=564, df 524; p=00001]. The FEV1 and FEV1/FVC was maintained within normal limits. 86% prescriptions included Lycopodium, Arsenicum album, Pulsatilla, Phosphorus, Stannum metallicum, Calcarea carbonica, Silicea, Bryonia alba. Conclusion: The result suggests effectiveness of homeopathic treatment in early years of SCMB patients. Controlled trials are warranted. (AU)


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Bronchitis, Chronic/therapy , Homeopathy , Pulsatilla nigricans/therapeutic use , Arsenicum Album/therapeutic use , Calcarea Carbonica/therapeutic use , Lycopodium clavatum/therapeutic use , Phosphorus , Silicea Terra/therapeutic use , Stannum Metallicum/therapeutic use , Bryonia
7.
Phytomedicine ; 50: 257-284, 2018 Nov 15.
Article in English | MEDLINE | ID: mdl-30466987

ABSTRACT

INTRODUCTION: Adaptogens are natural compounds or plant extracts that increase adaptability and survival of organisms under stress. Adaptogens stimulate cellular and organismal defense systems by activating intracellular and extracellular signaling pathways and expression of stress-activated proteins and neuropeptides. The effects adaptogens on mediators of adaptive stress response and longevity signaling pathways have been reported, but their stress-protective mechanisms are still not fully understood. AIM OF THE STUDY: The aim of this study was to identify key molecular mechanisms of adaptogenic plants traditionally used to treat stress and aging-related disorders, i.e., Rhodiola rosea, Eleutherococcus senticosus, Withania somnifera, Rhaponticum carthamoides, and Bryonia alba. MATERIALS AND METHODS: To investigate the underlying molecular mechanisms of adaptogens, we conducted RNA sequencing to profile gene expression alterations in T98G neuroglia cells upon treatment of adaptogens and analyzed the relevance of deregulated genes to adaptive stress-response signaling pathways using in silico pathway analysis software. RESULTS AND DISCUSSION: At least 88 of the 3516 genes regulated by adaptogens were closely associated with adaptive stress response and adaptive stress-response signaling pathways (ASRSPs), including neuronal signaling related to corticotropin-releasing hormone, cAMP-mediated, protein kinase A, and CREB; pathways related to signaling involving CXCR4, melatonin, nitric oxide synthase, GP6, Gαs, MAPK, neuroinflammation, neuropathic pain, opioids, renin-angiotensin, AMPK, calcium, and synapses; and pathways associated with dendritic cell maturation and G-coupled protein receptor-mediated nutrient sensing in enteroendocrine cells. All samples tested showed significant effects on the expression of genes encoding neurohormones CRH, GNRH, UCN, G-protein-coupled and other transmembrane receptors TLR9, PRLR, CHRNE, GP1BA, PLXNA4, a ligand-dependent nuclear receptor RORA, transmembrane channels, transcription regulators FOS, FOXO6, SCX, STAT5A, ZFPM2, ZNF396, ZNF467, protein kinases MAPK10, MAPK13, MERTK, FLT1, PRKCH, ROS1, TTN), phosphatases PTPRD, PTPRR, peptidases, metabolic enzymes, a chaperone (HSPA6), and other proteins, all of which modulate numerous life processes, playing key roles in several canonical pathways involved in defense response and regulation of homeostasis in organisms. It is for the first time we report that the molecular mechanism of actions of melatonin and plant adaptogens are alike, all adaptogens tested activated the melatonin signaling pathway by acting through two G-protein-coupled membrane receptors MT1 and MT2 and upregulation of the ligand-specific nuclear receptor RORA, which plays a role in intellectual disability, neurological disorders, retinopathy, hypertension, dyslipidemia, and cancer, which are common in aging. Furthermore, melatonin activated adaptive signaling pathways and upregulated expression of UCN, GNRH1, TLR9, GP1BA, PLXNA4, CHRM4, GPR19, VIPR2, RORA, STAT5A, ZFPM2, ZNF396, FLT1, MAPK10, MERTK, PRKCH, and TTN, which were commonly regulated by all adaptogens tested. We conclude that melatonin is an adaptation hormone playing an important role in regulation of homeostasis. Adaptogens presumably worked as eustressors ("stress-vaccines") to activate the cellular adaptive system by inducing the expression of ASRSPs, which then reciprocally protected cells from damage caused by distress. Functional investigation by interactive pathways analysis demonstrated that adaptogens activated ASRSPs associated with stress-induced and aging-related disorders such as chronic inflammation, cardiovascular health, neurodegenerative cognitive impairment, metabolic disorders, and cancer. CONCLUSION: This study has elucidated the genome-wide effects of several adaptogenic herbal extracts in brain cells culture. These data highlight the consistent activation of ASRSPs by adaptogens in T98G neuroglia cells. The extracts affected many genes playing key roles in modulation of adaptive homeostasis, indicating their ability to modify gene expression to prevent stress-induced and aging-related disorders. Overall, this study provides a comprehensive look at the molecular mechanisms by which adaptogens exerts stress-protective effects.


Subject(s)
Neuroglia/drug effects , Plant Extracts/pharmacology , Systems Biology , Adaptation, Physiological , Brain/cytology , Bryonia/chemistry , Cell Line, Tumor , Eleutherococcus/chemistry , Glioblastoma , Humans , Leuzea/chemistry , Longevity/drug effects , Rhodiola/chemistry , Signal Transduction/drug effects , Withania/chemistry
8.
Complement Ther Med ; 40: 185-190, 2018 Oct.
Article in English | MEDLINE | ID: mdl-30219446

ABSTRACT

BACKGROUND: In Europe only few integrative pediatric wards exists and there are two German hospitals focusing on anthroposophic medicine as part of complementary and alternative medicine (CAM). Whilst the most common pediatric diseases are treated here, pseudocroup patients make up a large group in these hospitals, receiving conventional as well as anthroposophic therapies. However, effectiveness of these therapy concepts mostly based on physicians' experiences but clinical studies are hitherto missing. METHODS: A systematic literature search identifying therapy approaches for pseudocroup in children was conducted in general electronic databases (Cochrane Library, PubMed, OVID) and in CAM-specific databases (CAMbase, CAM-QUEST®, Anthromedics). Search results were screened for anthroposophic therapy options. In addition, anthroposophic guidebooks were handsearched for relevant information. RESULTS: Among 157 articles fulfilling search criteria one retrospective study, and five experience reports describing anthroposophic treatments were identified. Several medications for the treatment of pseudocroup were mentioned such as Aconitum, Apis, Bryonia, Hepar sulfuris, Lavender, Pyrit, Sambucus and Spongia. During appropriate use no adverse effects were reported. CONCLUSION: Anthroposophic medicine harbors a broad spectrum of remedies for the treatment of pseudocroup in children. In particular, Aconitum, Bryonia and Spongia are frequently recommended; however, clinical trials investigating the effectiveness are sparse. Therefore, development and validation of therapy strategies are required.


Subject(s)
Anthroposophy , Croup/drug therapy , Laryngitis/drug therapy , Plant Extracts/therapeutic use , Aconitum/chemistry , Bryonia/chemistry , Child , Humans , Phytotherapy
9.
Homeopathy ; 107(4): 280-291, 2018 Nov.
Article in English | MEDLINE | ID: mdl-29908526

ABSTRACT

INTRODUCTION: The analysis of the periodic table of elements by Jan Scholten opened the way for a new kind of classification and repertorisation of homeopathic remedies. Thereby, group analysis (resorting to series and stages) makes precise prescriptions possible. This approach appears to yield striking results, even in severe cases. Whereas Hahnemann stressed the emotional state ('Gemüthssymptome', Organon § 210) when choosing a remedy, Scholten 200 years later investigated the mental picture that represents a life conflict or even a life theme that may maintain the disease process. The person's environment, emotional traumas or a conflict drives him or her to suppress and dissect painful emotions. Such compensations can become subconscious and so strong that they can no longer be controlled; they then influence the patient with a highly destructive energy. METHODS: We present five case reports, each dealing with an unusual clinical course of severe cancer associated with homeopathic treatment using the Scholten method. RESULTS: By presenting these cases, we consider how the constitution (lifelong signs and symptoms of the patient) and the mental state are interwoven and, as a complex mechanism, might provoke disease. CONCLUSION: The appropriate homeopathic remedy, reflecting the Scholten approach, seemed to have beneficial impact on the disease process of the five individuals presented.


Subject(s)
Homeopathy/methods , Neoplasms/drug therapy , Adult , Aged , Bone Neoplasms/drug therapy , Bone Neoplasms/psychology , Bryonia/metabolism , Calcium Compounds/therapeutic use , Female , Homeopathy/standards , Humans , Leukopenia/drug therapy , Leukopenia/psychology , Lung Neoplasms/drug therapy , Lung Neoplasms/psychology , Lymphoma, Non-Hodgkin/drug therapy , Lymphoma, Non-Hodgkin/psychology , Male , Mesothelioma/drug therapy , Mesothelioma/psychology , Middle Aged , Neoplasms/psychology , Oxides/therapeutic use , Phosphorus/therapeutic use , Thuja/metabolism
10.
São Paulo; s.n; 2017. 38 p.
Thesis in Portuguese | HomeoIndex Homeopathy | ID: hom-11868

ABSTRACT

A febre é um sinal clínico muito associado à infecção, gerando grande número de atendimentos em emergências e consultórios, e uso indiscriminado de antipiréticos.É desencadeada pela ação de citocinas pirogênicas endógenas e exógenas, desempenhando funções protetoras no organismo e auxiliando na recuperação. Conhecendo a importância da febre no mecanismo imunológico, e com a intenção de aliviar o desconforto do paciente, o medicamento homeopático tem como finalidade restabelecer a força vital, acelerando o processo de cura. Dentre todos os medicamentos homeopáticos, o simillimum é encontrado através da totalidade sintomática do paciente.Neste trabalho listamos alguns dos principais medicamentos utilizados para o tratamento da febre e suas indicações.(AU)


Fever is a clinical symptom generally associated to infection, generating a large number of emergency cases and medical appointments, and an indiscriminate use of antipyretics. The fever is triggered by the action of endogenous and exogenous pyrogenic cytokines, performing protective functions in the organism and aiding in the recovery. Regarding the importance of the fever in the immune mechanism, and with the intent to relieve the discomfort of the patient, the homeopathic medicine aims to restore the vital force, accelerating the healing process. Among all homeopathic medicines, simillimum is found through totality of symptoms manifested by the patient.(AU)


Subject(s)
Homeopathy , Fever/therapy , Aconitum , Apis mellifica , Atropa belladonna , Bryonia , Ferrum Phosphoricum , Gelsemium sempervirens , Mercurius Solubilis , Phosphorus , Toxicodendron , Sulphur
11.
J Oleo Sci ; 65(8): 655-61, 2016 Aug 01.
Article in English | MEDLINE | ID: mdl-27430383

ABSTRACT

The composition of the fatty acids of the roots and aerial parts of Aritolochia longa (Aristolacheae) and Bryonia dioïca (Cucurbutaceae) was analyzed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The oils extracted from the aerial parts of both species were rich in polyunsaturated fatty acids with the essential linolenic and linoleic acids being the most prominent compounds. Oleic and linoleic acids were the majors fatty acids in the roots of both species. Whatever the plant part analyzed and the species, the saturated fatty acids were predominantly composed of palmitic and stearic acids. The antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the lipid extracts were determined against a panel of five bacterial strains. The results showed that the sensitivity to the lipid extracts was different for the test bacterial strains, and the susceptibility of gram positive bacteria was found to be greater than gram negative bacteria. The antibacterial activity of the root lipid extracts was particularly important against Enterococcus feacium (CMI value of 125 µg/mL; CMB values > 250 µg/mL) and Streptococcus agalactiae (CMI value of 125 µg/mL; CMB values 250 µg/mL for A. longa roots). These results indicate that A. longa and B. dioïca could be considered as good sources of essential fatty acids which can act as natural antibacterial agents.


Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Aristolochia/chemistry , Aristolochia/growth & development , Bryonia/chemistry , Bryonia/growth & development , Fatty Acids/analysis , Anti-Bacterial Agents/isolation & purification , Dose-Response Relationship, Drug , Fatty Acids/chemistry , Fatty Acids/isolation & purification , Fatty Acids/pharmacology , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Microbial Sensitivity Tests , Plant Components, Aerial/chemistry , Plant Roots/chemistry , Structure-Activity Relationship , Tunisia
12.
Homeopatia Méx ; 84(695): 13-18, mar.-abr. 2015.
Article in Spanish | LILACS | ID: lil-762167

ABSTRACT

El presente trabajo tiene el objetivo de mostrar el efecto terapéutico de algunos medicamentos homeopáticos en la rinofaringitis aguda o resfriado común, enfermedad aguda de vías respiratorias superiores de tipo viral de baja patogenicidad (VRSBP) y gran incidencia en la actualidad, en una muestra piloto de tipo clínico, longitudinal y prospectiva, conformada por 22 pacientes captados en la Unidad Médica de la Escuela Nacional de Medicina y Homeopatía (ENMH) del Instituto Politécnico Nacional (IPN); 15 pacientes del sexo femenino y 7 del masculino, en un rango de entre 4 y 11 años de edad. La elección de los medicament s homeopáticos se realizó con base en lasemiología de los síntomas presentados, enfatizando particularmente en las modalidades, el color del moco, ataque al estado general y la exploración física.


This paper aims to show the therapeutic effect of some homeopathic medicines in acute nasopharyngitis or common cold , acute upper respiratory disease of viral type low pathogenic ( VRSBP ) and high incidence currently in a pilot sample clinical , longitudinal and prospective type , consisting of 22 patients recruited in the Medical Unit of the National School of Medicine and Homeopathy ( ENMH ) of the National Polytechnic Institute ( IPN) ; 15 female patients and 7 male , ranging between 4 and 11 years old. The choice of homeopathic medicament s was made based on thesemiotics of the symptoms presented , emphasizing particularly on the modalities , the color of mucus, malaise and physical examination.


Subject(s)
Humans , Male , Female , Child , Onions/therapeutic use , Bryonia/therapeutic use , Homeopathic Therapeutics , Nasopharyngitis/therapy , Respiratory Tract Diseases/therapy , Homeopathic Remedy , Virus Diseases
13.
J Ethnopharmacol ; 166: 250-60, 2015 May 26.
Article in English | MEDLINE | ID: mdl-25797117

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: Although acknowledged as toxic herbs, Aristolochia species are still widely used worldwide. The aristolochic acids (AA) they contain can induce the so-called "aristolochic acid nephropathy", leading to renal fibrosis and upper urinary tract cancer. Traditional Moroccan medicine still often uses Aristolochia species under the vernacular name of Bereztem for the treatment of numerous ailments, notably cancer, diabetes or digestive tract disorders. As the botanical identity and renal toxicity of used species remain unexplored, the safety of patients may be threatened. MATERIAL AND METHODS: Ethnopharmacological data were collected from herbalists from the provinces of Oujda and Berkane, located in North-Eastern Morocco. Samples of Bereztem were collected at herbalist shops and checked for their content in AA using TLC and LC-MS methods. The toxicity of crude methanolic extracts of each herb was assessed on a HK-2 cell-based in vitro model by measurement of the cell survival to evaluate cytotoxicity and by assessment of renal-specific toxicity via (i) the evaluation of genes (E-cadherin and α-smooth muscle actin) expression by RT-qPCR; (ii) the quantities of ß-catenin and vimentin by immunofluorescence microscopy; (iii) the secretion of fibronectin; and (iv) the excretion of interleukin-6. RESULTS: The survey indicated that, among 42 herbalists visited, 33 were retailers of Bereztem, which was generally sold as a cancer treatment. Botanical investigations revealed that Aristolochia longa was frequently substituted by Bryonia dioica, which was associated with a higher cytotoxicity. Parameters specific to renal toxicity were also found to be enhanced, as compared to Aristolochia baetica and A. longa: down-regulation of ß-catenin and E-cadherin and up-regulation of vimentin and α-smooth muscle actin, and secretion of fibronectin and interleukin-6. CONCLUSION: In accordance with the Moroccan regulations, the use of so-called Aristolochia species should be discontinued. On one hand, the correctly identified aristolochia contain nephrotoxic aristolochic acids; on the other hand, aristolochia are massively substituted in North-Eastern Morocco and adulterated by a well-known toxic herb, B. dioica. Our data indicate that the bryony renal toxicity may be deleterious in shorter time periods than aristolochia. Reinforced on-site controls are needed to remind herbalists and harvesters that these herbs should be prohibited.


Subject(s)
Aristolochia/adverse effects , Bryonia/adverse effects , Kidney Diseases/chemically induced , Kidney/drug effects , Plant Extracts/adverse effects , Plants, Medicinal/adverse effects , Actins/metabolism , Aristolochia/chemistry , Bryonia/chemistry , Cadherins/metabolism , Cell Line , Cell Survival/drug effects , Down-Regulation/drug effects , Ethnopharmacology/methods , Fibronectins/metabolism , Humans , Interleukin-6/metabolism , Kidney/metabolism , Kidney Diseases/metabolism , Morocco , Plant Extracts/chemistry , Plants, Medicinal/chemistry , Up-Regulation/drug effects , beta Catenin/metabolism
14.
Homeopatia Méx ; 84(695): 13-18, mar.-abr. 2015.
Article in Spanish | HomeoIndex Homeopathy | ID: hom-11096

ABSTRACT

El presente trabajo tiene el objetivo de mostrar el efecto terapéutico de algunos medicamentos homeopáticos en la rinofaringitis aguda o resfriado común, enfermedad aguda de vías respiratorias superiores de tipo viral de baja patogenicidad (VRSBP) y gran incidencia en la actualidad, en una muestra piloto de tipo clínico, longitudinal y prospectiva, conformada por 22 pacientes captados en la Unidad Médica de la Escuela Nacional de Medicina y Homeopatía (ENMH) del Instituto Politécnico Nacional (IPN); 15 pacientes del sexo femenino y 7 del masculino, en un rango de entre 4 y 11 años de edad. La elección de los medicament s homeopáticos se realizó con base en lasemiología de los síntomas presentados, enfatizando particularmente en las modalidades, el color del moco, ataque al estado general y la exploración física. (AU)


This paper aims to show the therapeutic effect of some homeopathic medicines in acute nasopharyngitis or common cold , acute upper respiratory disease of viral type low pathogenic ( VRSBP ) and high incidence currently in a pilot sample clinical , longitudinal and prospective type , consisting of 22 patients recruited in the Medical Unit of the National School of Medicine and Homeopathy ( ENMH ) of the National Polytechnic Institute ( IPN) ; 15 female patients and 7 male , ranging between 4 and 11 years old. The choice of homeopathic medicament s was made based on thesemiotics of the symptoms presented , emphasizing particularly on the modalities , the color of mucus, malaise and physical examination. (AU)


Subject(s)
Humans , Male , Female , Child , Nasopharyngitis/therapy , Onions/therapeutic use , Bryonia/therapeutic use , Homeopathic Therapeutics , Virus Diseases/drug therapy , Homeopathic Remedy , Respiratory Tract Diseases/therapy
15.
J Wound Care ; 23(9): 431-2, 434, 436 passim, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25284295

ABSTRACT

OBJECTIVE: Silver nanoparticles (AgNPs) are known for their antimicrobial profile and wound healing activities. However, cytotoxicity and cosmetic abnormalities associated with silver pose a major challenge in their translation for therapeutic applications. Our objective was to develop biogenic AgNPs, using a single-step green synthesis, and to investigate their in vitro and in vivo behaviour as wound-healing agents. METHOD: AgNPs were prepared using the green synthesis approach with aqueous Bryonia laciniosa leaves extract. The AgNPs were then evaluated for physicochemical properties, stability, and antimicrobial and in vivo wound healing activities. RESULTS: Stable AgNPs with characteristic absorption at 408nm and 15±3nm particle size were generated via the active involvement of Bryonia laciniosa. No loss of stability was detected after 6 months at room temperature. Antibacterial activity was observed against both Gram-negative and Gram-positive bacteria with no cytotoxicity observed in vitro at a concentration of 200 µg/mL and effective cytokine modulation. In vivo wound healing experiments showed improved wound contracting ability in rats where, after 14 days, wound alleviation was 47.1±2.2% in the control groups, compared with 78.1±1.4% and 92.6±6.7% for a silver-based marketed cream and the AgNPs, respectively. CONCLUSION: The developed AgNPs proved to be superior wound healing agents owing to scarless healing with insignificant inflammation and toxicity. DECLARATION OF INTEREST: There were no external sources of funding for this study. The authors have no conflicts of interest to declare.


Subject(s)
Metal Nanoparticles/therapeutic use , Silver/administration & dosage , Wound Healing , Animals , Bryonia , Enzyme-Linked Immunosorbent Assay , Metal Nanoparticles/chemistry , Plant Extracts , Rats
16.
Homeopatia Méx ; 83(688): 25-28, ene.-feb. 2014. ilus
Article in Spanish | LILACS | ID: lil-731453

ABSTRACT

Este artículo relata un caso clínico urgente de oligohidramnios (OH) severo, en una paciente primigesta que se encontraba en la semana 21 de gestación, y con un pronóstico de terminación de la misma en las siguientes 48 a 72 horas. La intervención con una prescripción compleja permitió la reversión pronta y total del OH hasta permitir la conclusión a término de un producto sano. Dada la carencia de bibliografía en esta patología, se describe la justificación de la conducta terapéutica.


This paper talks about a mild-severe, urgent case of oligohydramios (OH) in a 21 years woman on her first pregnancy, with a fatal prognosis during the next 48- 72 hours. Homeopathic intervention with a complex prescription produced a rapid and total reversion of OH, leading a term birth of a healthy boy. Due to lacking of homeopathic bibliography on this condition, it is presented rational of therapeutic conduct.


Subject(s)
Humans , Female , Pregnancy , Young Adult , Bryonia/therapeutic use , Homeopathy , Naja tripudians/therapeutic use , Oligohydramnios/therapy , Pregnancy Complications , Tarentula hispanica/therapeutic use
17.
Homeopatia Méx ; 83(688): 25-28, ene.-feb. 2014. ilus
Article in Spanish | HomeoIndex Homeopathy | ID: hom-10984

ABSTRACT

Este artículo relata un caso clínico urgente de oligohidramnios (OH) severo, en una paciente primigesta que se encontraba en la semana 21 de gestación, y con un pronóstico de terminación de la misma en las siguientes 48 a 72 horas. La intervención con una prescripción compleja permitió la reversión pronta y total del OH hasta permitir la conclusión a término de un producto sano. Dada la carencia de bibliografía en esta patología, se describe la justificación de la conducta terapéutica. (AU)


This paper talks about a mild-severe, urgent case of oligohydramios (OH) in a 21 years woman on her first pregnancy, with a fatal prognosis during the next 48- 72 hours. Homeopathic intervention with a complex prescription produced a rapid and total reversion of OH, leading a term birth of a healthy boy. Due to lacking of homeopathic bibliography on this condition, it is presented rational of therapeutic conduct. (AU)


Subject(s)
Humans , Female , Pregnancy , Young Adult , Homeopathy , Pregnancy Complications , Oligohydramnios/therapy , Tarentula hispanica/therapeutic use , Bryonia/therapeutic use , Naja tripudians/therapeutic use
18.
J Sci Food Agric ; 93(7): 1692-8, 2013 May.
Article in English | MEDLINE | ID: mdl-23152306

ABSTRACT

BACKGROUND: Wild vegetables have traditionally been consumed as part of the Mediterranean diet, being valuable sources of nutrients and bioactive compounds. The objective of this work was to analyse the carotenoid content of the edible young shoots of four species (Asparagus acutifolius L., Humulus lupulus L., Bryonia dioica Jacq. and Tamus communis L.) as part of a wider study on the characterisation of the nutritional composition of wild edible plants commonly consumed in Spain. Samples were gathered from two locations in Central Spain for two consecutive years. RESULTS: Lutein, ß-carotene, neoxanthin and violaxanthin were quantified by high-performance liquid chromatography with a photodiode array detector. Median concentration ranges (µg g(-1) edible wet weight) were: ß-carotene, 3.39-6.69, lutein, 5.44­19.13;neoxanthin, 5.17-17.37; and violaxanthin, 2.08-8.93. The highest carotenoid content was that of B. dioica (59.01 µg g(-1)) and the lowest was found in A. acutifolius (17.58 µg g(-1)) [corrected]. CONCLUSION: Our results show that these wild young shoots are richer sources of carotenoids than many of the commercially available leafy vegetables.


Subject(s)
Asparagus Plant/chemistry , Bryonia/chemistry , Diet , Humulus/chemistry , Tamus/chemistry , Xanthophylls/analysis , beta Carotene/analysis , Chromatography, High Pressure Liquid , Humans , Lutein/analysis , Plant Shoots/chemistry , Spain
19.
J Ethnopharmacol ; 141(1): 510-6, 2012 May 07.
Article in English | MEDLINE | ID: mdl-22465729

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: Bryonia dioica Jacq. is a climbing perennial herb with tuberous roots which is widely used in traditional medicine in Algeria for the treatment of cancers; it belongs to the genus Bryonia (Cucurbitaceae). AIM OF THE STUDY: To investigate the cytotoxic and apoptogenic activities, the phytochemical composition and acute toxicity of the aqueous extract of Bryonia dioica roots growing in Algeria. MATERIALS AND METHODS: Dried roots of Bryonia dioica were extracted with water (decoction). The cytotoxic effects of the aqueous extract in the Burkitt's lymphoma BL41 cell lines were evaluated by flow cytometry. Apoptosis induction was assessed by two corroborative assays; propidium iodide (PI) staining of cell DNA and flow cytometric light scatter analysis. The mitochondria membrane potential was investigated using a fluorescent dye DIOC6. The expression of caspases-3, -8, -9 and PARP was assessed by Western blot. The phytochemical screening of the roots of Bryonia dioica was performed using qualitative phytochemical standard procedures. RESULTS: The Bryonia dioica aqueous extract induced cell death in a dose-dependent manner. The IC50 of Bryonia dioica aqueous extract was estimated to be approximately 15, 63µg/ml. This was accompanied by induction of apoptosis, activation of caspase-3 and -9, cleavage of PARP and loss of mitochondria membrane potential. Furthermore, the phytochemical screening of roots of Bryonia dioica showed the presence of various bioactive such as polyphenols, sterols and triterpenes, alkaloids, c-heterosides, carbohydrates and saponins. CONCLUSION: The aqueous extract of Bryonia dioica induces apoptosis in the Burkitt's lymphoma BL41 cell lines via the mitochondrial pathway. The flavonoids, sterols and triterpens detected could be responsible for the cytotoxic and apoptogenic activities of the aqueous extract of Bryonia dioica. These findings suggest that Bryonia dioica could be considered as a promising source for developing novel therapeutics against Burkitt's lymphoma.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Apoptosis/drug effects , Bryonia , Burkitt Lymphoma/pathology , Mitochondria/drug effects , Plant Extracts/pharmacology , Signal Transduction/drug effects , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/toxicity , Blotting, Western , Bryonia/chemistry , Burkitt Lymphoma/metabolism , Caspase 3/metabolism , Caspase 9/metabolism , Cell Line, Tumor , Dose-Response Relationship, Drug , Flow Cytometry , Humans , Inhibitory Concentration 50 , Light , Membrane Potential, Mitochondrial/drug effects , Mitochondria/metabolism , Mitochondria/pathology , Phytotherapy , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Plant Extracts/toxicity , Plant Roots , Plants, Medicinal , Poly(ADP-ribose) Polymerases/metabolism , Scattering, Radiation , Solvents/chemistry , Water/chemistry
20.
Int. j. high dilution res ; 10(35): 75-76, june 30, 2011.
Article in English | LILACS-Express | ID: hom-10662

ABSTRACT

Paracelsus once wrote: "All things are poison and nothing is without poison, only the dose permits something not to be poisonous." Latter Hahnemann formulated the law of similars, preparations which cause certain symptoms in healthy individuals if given in diluted form to patients exhibiting similar symptoms will cure it. Highly diluted natural complexes prepared according to Hahnemann?s ancient techniques may represent a new form of immunomodulatory therapy. The lack of scientific research with highly diluted products led us to investigate the in vivo and in vitro actions of commonly used medications. Here we describe the results of experimental studies aimed at verifying the effects of Mercurius solubilis, Atropa Belladonna, Lachesis muta and Bryonia alba. All medications were at 200cH dilution. Animals were maintained for 7 days and were allowed to drink the medications, which were prepared in a way that the final dilution and agitation (200cH) was performed in drinking water. The medication bottle was changed and sucussed every afternoon. Coculture of non treated mice bone marrow cells and in vitro treated peritoneal macrophages were also performed. After animal treatment the bone marrow cells were immunophenotyped with hematopoietic lineage markers on a flow cytometer. We have determined CD11b levels on bone marrow cells after culture and co-culture with treated macrophages and these macrophages were processed to scanning electron microscopy. We have observed by morphological changes that macrophages were activated after all treatments. Mercurius solubilis treated mice showed an increase in CD3 expression and in CD11b on nonadherent bone marrow cells after co-culture with in vitro treatment. Atropa Belladonna increased CD45R and decreased Ly-6G expression on bone marrow cells after animal treatment. Lachesis muta increased CD3, CD45R and, CD11c expression and decreased CD11b ex vivo and in nonadherent cells from co-culture. Bryonia alba increased Ly-6G, CD11c and CD11b expression ex vivo and when in co-culture CD11b was increased in adherent cells as well as decreased in nonadherent cells. With these results we have demonstrated that highly diluted medications act on immune cells activating macrophages, and changing the expression profile of hematopoietic lineage markers. Highly diluted medications are less toxic and cheaper than other commonly used medications and based on our observations, it is therefore conceivable that this medications which are able to act on bone marrow and immune cells may have a potential therapeutic use in clinical applications in diseases were the immune system is affected and also as regenerative medicine as it may allow proliferation and differentiation of progenitor cells. (AU)


Subject(s)
High Potencies , Bone Marrow , Macrophages , Mercurius Solubilis , Atropa belladonna , Lachesis muta , Bryonia
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