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1.
Methods Mol Biol ; 2065: 65-77, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31578688

RESUMEN

The recent resurgence of yellow fever virus (YFV) activity in the tropical regions of Africa and South America has sparked renewed interest in this infamous arboviral disease. Also, the development and production of viral vaccines involve several steps that need the monitoring of viral load throughout the process (antigen production, purification, and inactivation). Currently, these steps are followed by plaque lysis titration assay, whose results take about 7-10 days to come out and thus resulting in a laborious and time-consuming approach. With the advent of quantitative real-time PCR (qPCR), we have a faster method to be applied during vaccine production and also to be effectively used for the diagnosis of YFV infection. The technique herein standardized proved to be effective for determining YF viral load both in vivo and in vitro, thus becoming a very important tool for laboratory analysis to verify the vaccination status of individuals, beyond acting as a quality control for vaccine production and diagnosis.

2.
Pediatr Infect Dis J ; 2019 Nov 07.
Artículo en Inglés | MEDLINE | ID: mdl-31725551

RESUMEN

Yellow fever is an endemic disease in tropical areas in America and Africa. We report a case where the wild-type yellow fever virus was detected in a breast milk sample of a 33-year-old woman, from a rural area in the municipality of São Paulo, thus highlighting a potential risk for transmission of yellow fever virus through breast-feeding.

3.
Rev Inst Med Trop Sao Paulo ; 61: e35, 2019 Jul 22.
Artículo en Inglés | MEDLINE | ID: mdl-31340247

RESUMEN

Yellow fever is one of the most important mosquito-borne diseases, which still affects a significant number of people every year, mainly in tropical countries. Mortality can be high, even with intensive treatment due to multiple organ failure, including acute kidney injury (AKI). This disease can also be a burden on the health care system in developing countries, without mentioning the number of lives that could be spared with an early diagnosis and adequate monitoring and treatment. The pathophysiology of yellow fever-induced acute kidney injury (AKI) is still to be completely understood, and the best clinical approach has not yet been determined. This manuscript presents the most recent scientific evidence of kidney involvement in yellow fever, since AKI plays an important role in the mortality rate. Recent outbreaks have occurred in Brazil and further studies are required to provide a better clinical control for patients with yellow fever.


Asunto(s)
Lesión Renal Aguda/virología , Fiebre Amarilla/complicaciones , Brasil , Humanos , Estaciones del Año , Fiebre Amarilla/diagnóstico , Fiebre Amarilla/tratamiento farmacológico , Fiebre Amarilla/prevención & control
4.
Korean J Parasitol ; 57(3): 283-290, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31284351

RESUMEN

A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.


Asunto(s)
Pruebas Diagnósticas de Rutina/métodos , Proteínas no Estructurales Virales/análisis , Fiebre Amarilla/diagnóstico , Virus de la Fiebre Amarilla/aislamiento & purificación , Animales , Anticuerpos Antivirales/análisis , Anticuerpos Antivirales/inmunología , Femenino , Haplorrinos , Humanos , Inmunización , Ratones , Sensibilidad y Especificidad , Proteínas no Estructurales Virales/genética , Proteínas no Estructurales Virales/inmunología , Fiebre Amarilla/sangre , Fiebre Amarilla/inmunología , Fiebre Amarilla/virología , Virus de la Fiebre Amarilla/genética , Virus de la Fiebre Amarilla/inmunología , Virus de la Fiebre Amarilla/fisiología
5.
Nat Med ; 25(8): 1218-1224, 2019 08.
Artículo en Inglés | MEDLINE | ID: mdl-31308506

RESUMEN

Flaviviral infections result in a wide spectrum of clinical outcomes, ranging from asymptomatic infection to severe disease. Although the correlates of severe disease have been explored1-4, the pathophysiology that differentiates symptomatic from asymptomatic infection remains undefined. To understand the molecular underpinnings of symptomatic infection, the blood transcriptomic and metabolomic profiles of individuals were examined before and after inoculation with the live yellow fever viral vaccine (YF17D). It was found that individuals with adaptive endoplasmic reticulum (ER) stress and reduced tricarboxylic acid cycle activity at baseline showed increased susceptibility to symptomatic outcome. YF17D infection in these individuals induced maladaptive ER stress, triggering downstream proinflammatory responses that correlated with symptomatic outcome. The findings of the present study thus suggest that the ER stress response and immunometabolism underpin symptomatic yellow fever and possibly even other flaviviral infections. Modulating either ER stress or metabolism could be exploited for prophylaxis against symptomatic flaviviral infection outcome.


Asunto(s)
Estrés del Retículo Endoplásmico , Vacuna contra la Fiebre Amarilla/inmunología , Fiebre Amarilla/metabolismo , Adulto , Ciclo del Ácido Cítrico , Susceptibilidad a Enfermedades , Humanos , Persona de Mediana Edad , Especies Reactivas de Oxígeno/metabolismo , Vacunas Atenuadas/inmunología , Fiebre Amarilla/etiología
6.
PLoS Negl Trop Dis ; 13(7): e0007625, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31329590

RESUMEN

BACKGROUND: New strategies for collecting post-mortem tissue are necessary, particularly in areas with emerging infections. Minimally invasive autopsy (MIA) has been proposed as an alternative to conventional autopsy (CA), with promising results. Previous studies using MIA addressed the cause of death in adults and children in developing countries. However, none of these studies was conducted in areas with an undergoing infectious disease epidemic. We have recently experienced an epidemic of yellow fever (YF) in Brazil. Aiming to provide new information on low-cost post-mortem techniques that could be applied in regions at risk for infectious outbreaks, we tested the efficacy of ultrasound-guided MIA (MIA-US) in the diagnosis of patients who died during the epidemic. METHODOLOGY/PRINCIPAL FINDINGS: In this observational study, we performed MIA-US in 20 patients with suspected or confirmed YF and compared the results with those obtained in subsequent CAs. Ultrasound-guided biopsies were used for tissue sampling of liver, kidneys, lungs, spleen, and heart. Liver samples from MIA-US and CA were submitted for RT-PCR and immunohistochemistry for detection of YF virus antigen. Of the 20 patients, 17 had YF diagnosis confirmed after autopsy by histopathological and molecular analysis. There was 100% agreement between MIA-US and CA in determining the cause of death (panlobular hepatitis with hepatic failure) and main disease (yellow fever). Further, MIA-US obtained samples with good quality for molecular studies and for the assessment of the systemic involvement of the disease. Main extrahepatic findings were pulmonary hemorrhage, pneumonia, acute tubular necrosis, and glomerulonephritis. One patient was a 24-year-old, 27-week pregnant woman; MIA-US assessed the placenta and provided adequate placental tissue for analysis. CONCLUSIONS: MIA-US is a reliable tool for rapid post-mortem diagnosis of yellow fever and can be used as an alternative to conventional autopsy in regions at risk for hemorrhagic fever outbreaks with limited resources to perform complete diagnostic autopsy.

7.
Recurso de Internet en Portugués | LIS - Localizador de Información en Salud, LIS-bvsms | ID: lis-LISBR1.1-46581

RESUMEN

A Febre do Mayaro é uma doença infecciosa febril aguda, cujo quadro clínico geralmente é de curso benigno, semelhante à Dengue e à Chikungunya. A Febre do Mayaro compõe a lista nacional de doenças de notificação compulsória imediata, conforme Portaria de Consolidação nº 4, de 28 de setembro de 2017.


Asunto(s)
Dengue , Fiebre Chikungunya , Infecciones por Arbovirus , Togaviridae , Infecciones por Alphavirus , Fiebre Amarilla
8.
Travel Med Infect Dis ; 30: 25-31, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31075425

RESUMEN

BACKGROUND: We study the association between prior yellow fever immunization and clinical outcomes of dengue infections in individuals of varying sexes and ages. Serological interactions between dengue virus and other flaviviruses could drive antibody dependent enhancement, which is associated with disease severity in dengue infections. This effect may influence disease severity in individuals subsequently affected by related flaviviruses, such as dengue. We compare the severity of dengue episodes between patients vaccinated and non-vaccinated against yellow fever. METHODS: We evaluated the severity of 11,448 lab-confirmed dengue cases reported in São José do Rio Preto, Brazil, in 7370 YF vaccinated patients compared to 4043 unvaccinated patients. We regressed dengue severity against YF vaccine status and a number of demographic, clinical, and laboratory variables as controls. We also evaluated the association between YF vaccination status and the clinical and laboratory symptoms of dengue patients. RESULTS: We did not find any evidence of increased risk for severe dengue in patients vaccinated against YF (odds ratio = 1.00; 95% confidence interval = 0.87-1.14). Most of the variables analyzed did not have a statistically significant association with YF vaccination status. CONCLUSIONS: We found no evidence that YF vaccination in dengue-endemic areas increases the risk of severe dengue fever.


Asunto(s)
Dengue/patología , Vacuna contra la Fiebre Amarilla/inmunología , Adolescente , Adulto , Brasil/epidemiología , Niño , Demografía , Dengue/diagnóstico , Dengue/epidemiología , Dengue/inmunología , Femenino , Humanos , Masculino , Estudios Retrospectivos , Factores de Riesgo , Índice de Severidad de la Enfermedad , Vacuna contra la Fiebre Amarilla/normas
9.
J Med Primatol ; 48(4): 211-217, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31032984

RESUMEN

BACKGROUND: Free-ranging non-human primates (NHPs) can host a variety of pathogenic microorganisms, such as arboviruses, which include the yellow fever virus (YFV). This study aimed to detect the circulation of YF and other arboviruses in three wild Alouatta caraya populations in forests in southern Brazil. METHODS: We collected 40 blood and serum samples from 26 monkeys captured/recaptured up to four times from 2014 to 2016, searching for evidence of arboviruses by virus isolation, PCR, and neutralization tests. RESULTS: Viral isolation and genome detection were negative; however, we detected neutralizing antibodies against the Saint Louis, Ilhéus, and Icoaraci viruses in three NHPs. CONCLUSIONS: Saint Louis Encephalitis, Ilhéus, and Icoaraci viruses circulated recently in the region. Future studies should investigate the role of NHPs, other vertebrate hosts and wild vectors in the region's arbovirus circulation and the potential risks of the arboviruses to wildlife, domestic animals, and humans.

10.
J Med Entomol ; 56(4): 1154-1158, 2019 Jun 27.
Artículo en Inglés | MEDLINE | ID: mdl-30927005

RESUMEN

The Togolese Republic has a tropical and humid climate which constitutes an ideal environment for mosquitoes to breed and transmit diseases. The Aedes mosquito is known to transmit yellow fever (YF), dengue, chikungunya, and Zika viruses in West Africa. Togo has been suffering from YF virus transmission, despite vaccination efforts. Unfortunately, there is scarcity in the data that reflect mosquito spatial distribution in Togo, specifically possible YF vectors. In the current study, mosquito surveillance efforts targeted areas with confirmed YF cases between July and August 2012. Indoor mosquitoes were collected using knockdown insecticide spraying, whereas Biogents (BG) traps were used to collect outdoor mosquito adults. Mosquito larval surveillance was conducted as well. In total, 17 species were identified. This investigation revealed the presence of medically important vectors in Togo, especially the Aedes aegypti (Linnaeus) (Diptera: Culicidae) which was collected in the four regions. Screening of all pools of female Aedes mosquitoes for YF, by real-time PCR, showed negative results. This is the first record for Coquillettidia flavocincta (Edwards) (Diptera: Culicidae) species in West Africa. This preliminary work serves as a baseline for further mosquito distribution studies in Togo.

11.
FP Essent ; 476: 11-17, 2019 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-30615405

RESUMEN

Due to rapid globalization and ease of travel, mosquito-borne viral infections are now a concern for family physicians throughout the United States. Zika virus infection is one such concern. It is spread via mosquito bites or by sexual contact with an infected individual. Most patients are asymptomatic, and when symptoms occur, they are mild and nonspecific. The main concern is the potential of the infection to cause fetal anomalies. Dengue is another mosquito-borne viral infection. Symptoms of initial infection are mild, and may include arthralgias. Subsequent infection with a different serotype can cause life-threatening hemorrhagic fever or shock. Chikungunya virus infection is widespread in the Americas and symptoms are similar to those of dengue. However, it can cause a postviral chronic inflammatory rheumatism in up to half of patients. Yellow fever occurs mostly in sub-Saharan Africa and can cause hepatic failure. Encephalitis viruses, most commonly West Nile in the United States and others such as Japanese encephalitis virus, can cause neuroinvasive disease, most often in older adults. Vaccines are available for yellow fever and Japanese encephalitis viruses but the keys to prevention are insect avoidance, mosquito eradication, and use of mosquito repellants.


Asunto(s)
Culicidae , Dengue , Fiebre Amarilla , Infección por el Virus Zika , Animales , Dengue/diagnóstico , Dengue/terapia , Dengue/transmisión , Humanos , Estados Unidos , Fiebre Amarilla/diagnóstico , Fiebre Amarilla/terapia , Fiebre Amarilla/transmisión , Infección por el Virus Zika/diagnóstico , Infección por el Virus Zika/terapia , Infección por el Virus Zika/transmisión
12.
JAMA Ophthalmol ; 137(3): 300-304, 2019 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-30629101

RESUMEN

Importance: Yellow fever virus (YFV) is a reemerging, potentially lethal arboviral disease that has been occurring recently in Africa and South America. Poor levels of immunization have facilitated the viral spread in southeastern Brazil, leading to an unprecedented outbreak that started in late 2016. Although human cases have been linked to sylvatic mosquitoes, the concern is that YFV may spread to urban centers infested with Aedes aegypti and Aedes albopictus mosquitoes and start a true urban cycle. Objective: To describe the ocular findings in patients with acute YFV infection. Design, Setting, Participants: Two adults with an acute YFV infection in southeastern Brazil underwent an ophthalmologic and ocular ultrasonographic examination in early 2018. Main Outcomes and Measures: Ocular findings in patients with acute YFV infection. Results: Both patients presented with increased choroidal thickness bilaterally seen on ocular ultrasonography. A man in his late 50s who had not been vaccinated previously also presented with bilateral, midperipheral, 360° choroidal detachment and yellowish subretinal lesions. After clinical deterioration and liver transplant, the man died. A woman in her early 30s who had been vaccinated previously for YFV presented with increased retinal venous congestion bilaterally. She was discharged with mild conjunctival chemosis and icterus. Conclusions and Relevance: These reports describe different patterns of ocular findings associated with YFV acute infection. However, the exact mechanism involved in the retinal and choroidal findings remains unclear.


Asunto(s)
Coroides/patología , Retina/patología , Fiebre Amarilla/patología , Adulto , Animales , Femenino , Humanos , Masculino , Persona de Mediana Edad
13.
PLoS Negl Trop Dis ; 12(12): e0007029, 2018 12.
Artículo en Inglés | MEDLINE | ID: mdl-30532188

RESUMEN

BACKGROUND: Between December 2015 and July 2016, a yellow fever (YF) outbreak affected urban areas of Angola and the Democratic Republic of the Congo (DRC). We described the outbreak in DRC and assessed the accuracy of the YF case definition, to facilitate early diagnosis of cases in future urban outbreaks. METHODOLOGY/PRINCIPAL FINDINGS: In DRC, suspected YF infection was defined as jaundice within 2 weeks after acute fever onset and was confirmed by either IgM serology or PCR for YF viral RNA. We used case investigation and hospital admission forms. Comparing clinical signs between confirmed and discarded suspected YF cases, we calculated the predictive values of each sign for confirmed YF and the diagnostic accuracy of several suspected YF case definitions. Fifty seven of 78 (73%) confirmed cases had travelled from Angola: 88% (50/57) men; median age 31 years (IQR 25-37). 15 (19%) confirmed cases were infected locally in urban settings in DRC. Median time from symptom onset to healthcare consultation was 7 days (IQR 6-9), to appearance of jaundice 8 days (IQR 7-11), to sample collection 9 days (IQR 7-14), and to hospitalization 17 days (IQR 11-26). A case definition including fever or jaundice, combined with myalgia or a negative malaria test, yielded an improved sensitivity (100%) and specificity (57%). CONCLUSIONS/SIGNIFICANCE: As jaundice appeared late, the majority of cases were diagnosed too late for supportive care and prompt vector control. In areas with known local YF transmission, a suspected case definition without jaundice as essential criterion could facilitate earlier YF diagnosis, care and control.


Asunto(s)
Fiebre Amarilla/epidemiología , Adulto , Angola , República Democrática del Congo/epidemiología , Brotes de Enfermedades , Femenino , Humanos , Masculino , Viaje , Población Urbana , Fiebre Amarilla/diagnóstico , Fiebre Amarilla/virología , Virus de la Fiebre Amarilla/genética , Virus de la Fiebre Amarilla/aislamiento & purificación , Virus de la Fiebre Amarilla/fisiología
14.
Sheng Wu Gong Cheng Xue Bao ; 34(12): 2025-2034, 2018 Dec 25.
Artículo en Chino | MEDLINE | ID: mdl-30584713

RESUMEN

Ebola virus (EBOV) is an extremely contagious pathogen first discovered in Africa associated with severe hemorrhagic disease in humans and nonhuman primates, which has resulted in at least 28 500 suspected cases and 11 300 confirmed deaths in 2014-2016 Ebola epidemic in West Africa. Rapid and sensitive detection of EBOV is the key to increasing the probability of survival and reducing infection rates in pandemic regions. Here, we report an ultrasensitive and instrument-free EBOV detection assay based on colloidal carbon immunochromatography. Carbon nanoparticle-labeled rabbit anti-EBOV-VP40 IgG were concentrated in the conjugate pad, monoclonal antibody (McAb, 4B7F9) against EBOV-VP40 and goat anti-rabbit IgG were immobilized on the nitrocellulose membrane with 2 µL/cm at a concentration of 1 mg/mL as test and control lines, respectively. Then the sample application pad, conjugate release pad, nitrocellulose membrane and absorbent pad were assembled into a lateral flow test strip. The test strip shows strong specificity against related viruses that share similar clinical symptoms and geographic range with EBOV, including marburg virus, influenza virus, yellow fever virus and dengue virus. In addition, 1 500 negative serums were tested with false-positive rate of 1.3‰ which significantly lower than that of ReEBOV™ colloidal gold test kit recommended by World Health Organization (WHO). The sensitivity of this strip was analyzed using inactivated EBOV with detection limit of 100 ng/mL (106 copies/mL) which clearly higher than that of ReEBOV™ dipstick (108 copies/mL). Furthermore, the strip showed excellent thermal stability characteristics in room temperature and could be as a point-of-care (POC), ultra-sensitive and specific promising candidate for EBOV serological screening in rural Africa or entry/exit ports.


Asunto(s)
Ebolavirus , Fiebre Hemorrágica Ebola , Nanopartículas , Animales , Carbono , Humanos , Conejos
15.
PLoS One ; 13(12): e0208907, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30566466

RESUMEN

Dengue fever is known to be one of the most common arthropod-borne viral infectious diseases of public health importance. The disease is now endemic in more than 100 countries in Africa, the Americas, the Eastern Mediterranean, Southeast Asia and the Western Pacific with an estimated two fifths of the world's population being at risk. The notable endemic viral hemorrhagic fevers (VHFs) found in West Africa, including yellow fever, Lassa fever, Rift Valley fever, dengue fever and until recently Ebola have been responsible for most outbreaks with fatal consequences. These VHFs usually produce unclear acute febrile illness, especially in the acute phase of infection. In this study we detected the presence of 2 different serotypes (DENV-2 and DENV-3) of Dengue virus in 4 sera of 150 patients clinically suspected of Ebola virus disease during the Ebola Virus Disease (EVD) outbreak in West Africa with the use of serological and molecular test assays. Sequence data was successfully generated for DENV-3 and phylogenetic analysis of the envelope gene showed that the DENV-3 sequences had close homology with DENV-3 sequences from Senegal and India. This study documents molecular evidence of an indigenous Dengue fever viral infection in Ghana and therefore necessitates the need to have an efficient surveillance system to rapidly detect and control the dissemination of the different serotypes in the population which has the potential to cause outbreaks of dengue hemorrhagic fevers.


Asunto(s)
Virus del Dengue/genética , Dengue , Ebolavirus/genética , Fiebre Hemorrágica Ebola , Dengue/epidemiología , Dengue/genética , Dengue/virología , Brotes de Enfermedades , Femenino , Ghana/epidemiología , Fiebre Hemorrágica Ebola/epidemiología , Fiebre Hemorrágica Ebola/genética , Fiebre Hemorrágica Ebola/virología , Humanos , Masculino
16.
Artículo en Inglés | MEDLINE | ID: mdl-30431406

RESUMEN

Tick-borne encephalitis virus (TBEV) can cause fever, headache, neurological disorders, and/or peripheral flaccid paralysis; therefore, it is a major threat to public health. A rapid, sensitive, and simple method for detecting anti-TBEV antibodies is needed urgently to determine infection and for vaccine evaluation. Here, a luciferase-based immunocomplex assay system (Luc-IC) was developed to detect TBEV antibodies. The system is based on a reporter Nano luciferase (NLuc) that is co-expressed as a fusion protein with viral envelope domain III (ED3) in COS7 cells. The cell supernatant was used directly to detect antigen without the need for a purification step. This simple procedure effectively improved the sensitivity of the assay. Sera from 50 patients with an acute tick-borne encephalitis infection were tested to determine the sensitivity of the NLuc-IC assay. Furthermore, 62 sera from individuals infected with Japanese encephalitis virus, West Nile virus, yellow fever virus, dengue virus, or Zika virus were also tested to determine specificity. The results demonstrated that the assay was 100% sensitive and 100% specific for TBEV antibodies. Thus, this very simple NLuc-IC assay is potentially useful for rapid and accurate diagnosis of TBEV infection in both humans and animals.

17.
J. Bras. Patol. Med. Lab. (Online) ; 54(5): 296-305, Sept.-Oct. 2018. tab, graf
Artículo en Inglés | LILACS-Express | ID: biblio-975848

RESUMEN

ABSTRACT Yellow fever is an infectious disease of acute evolution, initially non-contagious, transmitted by a ribonucleic acid (RNA) virus that belongs to the Flaviviridae family. In the period from December 2016 until March 17, 2017, 1, 561 suspected cases of wild yellow fever were reported to the Ministry of Health in Brazil. Among these cases, 850 (54.8%) remain under investigation, 448 (28.7%) were confirmed and 263 (16.9%) were discarded. Out of the total cases reported, 264 died, 144 (54.5%) were confirmed for the disease, 110 (41.7%) were investigated and 10 (3.8%) were discarded. The case fatality rate among confirmed cases was 32.2%. The specific diagnosis for determining the etiology of infection can be made by demonstrating the humoral response of the antibodies, virus isolation, or histopathological study of the liver. Only through early laboratory diagnosis and epidemiological data supply can government and cooperative organizations establish public policies to combat future disease epidemics, as well as social awareness campaigns.


RESUMO A febre amarela é uma doença infecciosa de evolução aguda, a princípio não contagiosa, transmitida por um vírus do ácido ribonucleico (RNA) que pertence à família Flaviviridae. No período de dezembro de 2016 a 17 de março de 2017, foram notificados ao Ministério da Saúde, 1.561 casos suspeitos de febre amarela silvestre no Brasil. Destes, 850 (54, 8%) permanecem em investigação; 448 (28, 7%) foram confirmados e 263 (16, 9%), descartados. Do total dos casos notificados, 264 evoluíram para óbito, sendo 144 (54, 5%) confirmados para a doença; 110 (41, 7%) em investigação e 10 (3, 8%), descartados. A taxa de letalidade entre os casos confirmados foi de 32, 2%. O diagnóstico específico para determinação da etiologia da infecção pode ser feito por meio da demonstração da resposta humoral dos anticorpos, do isolamento do vírus ou do estudo histopatológico do fígado. Apenas mediante o diagnóstico laboratorial precoce e o abastecimento de dados epidemiológicos é que governo e organizações cooperativas poderão estabelecer políticas públicas de combate a futuras epidemias da doença, bem como campanhas de conscientização social.

18.
J Travel Med ; 25(1)2018 01 01.
Artículo en Inglés | MEDLINE | ID: mdl-30184173
19.
Autops. Case Rep ; 8(3): e2018038, July-Sept. 2018. ilus
Artículo en Inglés | LILACS | ID: biblio-911892

RESUMEN

Disseminated mycosis (DM)­with cardiac involvement and shock­is an unexpected and severe opportunistic infection in patients with yellow fever. DM can mimic bacterial sepsis and should be considered in the differential diagnosis of causes of systemic inflammatory response syndrome in this group of patients, especially in areas where an outbreak of yellow fever is ongoing. We report the case of a 53-year-old male patient who presented to the emergency department with fever, myalgia, headache, and low back pain. The laboratory investigation revealed a positive molecular test for yellow fever, hepatic injury, and renal failure. During hospitalization, the patient developed hepatic encephalopathy, ascending leukocytosis, and ascites, with signs consistent with peritonitis. On the 11th day of hospitalization, the patient developed atrioventricular block, shock and died. At autopsy, angioinvasive mycosis was evidenced mainly in the heart, lungs, kidneys, and adrenals.


Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Infecciones Fúngicas Invasoras/complicaciones , Fiebre Amarilla/complicaciones , Autopsia , Diagnóstico Diferencial , Resultado Fatal , Infecciones Fúngicas Invasoras/patología , Riñón/lesiones , Insuficiencia Renal/complicaciones
20.
Sanid. mil ; 74(3): 151-157, jul.-sept. 2018. graf, tab
Artículo en Español | IBECS | ID: ibc-182292

RESUMEN

Antecedentes: Los virus causan enfermedades en el hombre como la gripe, la rabia, la fiebre amarilla o la fiebre hemorrágica. Además, presentan características como alta variabilidad genética, virulencia y fácil transmisión y producción con infraestructuras mínimas, lo que les convierte en un problema de salud pública y de bioseguridad. Por todo ello, desarrollar sistemas de biodetección precisos y versátiles es un reto ante el cual, la tecnología de micromatrices de ADN, se presenta como un sistema de ideal. Objetivos: Diseño de oligonucleótidos sonda para la detección de especies pertenecientes a nueve familias de virus de interés en biodefensa. Material y Métodos: Se seleccionaron familias de virus de interés en biodefensa, se buscaron los genomas completos de los virus de referencia de cada una de ellas en las bases de datos (GenBank) y se identificaron fragmentos de 60 nucleótidos que cumplieran determinados condicionantes estructurales, evaluándose con BLASTN su capacidad de hibridación cruzada. Resultados: Se obtuvieron los genomas de referencia de virus pertenecientes a nueve familias. Se diseñaron un total de 54 nucleótidos sonda, seis de ellos correspondientes al virus de la gripe A tipo H1N1 y se clasificaron las secuencias según su índice de identidad, permitiendo predecir la capacidad diagnóstica de las sondas diseñadas. Conclusiones: Se han encontrado secuencias suficientes para identificar nueve familias de virus, de interés en la biodefensa, mediante la tecnología de hibridación de micromatrices de ADN


Background: Viruses cause human diseases such as influenza, rage, yellow fever and hemorrhagic fever. In addition, they have characteristics such as high genetic variability, virulence and easy transmission and production with minimum level of infrastructure, which make them not only a public health but also a biosecurity problem. Therefore, the development of accurate and versatile biodetection systems is a challenge in which DNA microarray technology is released as an ideal system. Objectives: Probe design for virus detection by microarray technology. Materials and Methods: Virus families with interest in biodefense were selected and the complete genomes of the reference viruses were searched in the databases (GenBank). Fragments of 60 nucleotides with some specific physical characteristics were identified. Finally, cross-hybridization capacity was also evaluated with BLASTN software. Results: Viral genomes from nine families were obtained. A total of 54 probe nucleotides were designed, six of them corresponding to influenza A type H1N1 virus and the sequences were classified according to their identity index, allowing to predict the diagnostic capacity of the designed probes. Conclusion: It has been found enough sequences to identify nine virus families with interest in biodefense by means of the DNA microarray technology


Asunto(s)
Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Virus/aislamiento & purificación , Sondas de Oligonucleótidos , Análisis de Secuencia por Matrices de Oligonucleótidos/instrumentación , Virus/genética , Hibridación Genética
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