RESUMEN
A Gram-stain negative, aerobic, rod-shaped, motile by a single polar flagellum, non-spore-forming bacterium, designated strain AL-54T, was isolated from the storage liquid in the stems of Populus euphratica tree at the ancient Ugan River in Xinjiang, PR China. Isolated AL-54T grew optimally at pH 7.0 and temperature 35 °C in the presence of 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence demonstrated that the isolate belonged to the genus Pseudomonas and was closely related to Pseudomonas songnenensis NEAU-ST5-5 T (97.6%), Pseudomonas zhaodongensis NEAU-ST5-21 T (97.5%), Pseudomonas alcaliphila AL15-21T (97.3%), Pseudomonas toyotomiensis HT-3T (97.3%), Pseudomonas oleovorans subsp. lubricantis RS1T (97.3%), Pseudomonas stutzeri ATCC 17588T (97.3%), Pseudomonas chengduensis CGMCC 2318T (97.2%), and Pseudomonas xanthomarina KMM 1447T (97.1%). Multilocus Sequences Analysis (MLSA) of strain AL-54T based on the three housekeeping genes, rpoB, rpoD and gyrB further confirmed the phylogenetic assignment of the isolates. The G+C content was 64.7 mol%. The DNA-DNA hybridization with P. songnenensis NEAU-ST5-5 T, P. zhaodongensis NEAU-ST5-21T, P. alcaliphila AL15-21T, P. toyotomiensis HT-3T, P. oleovorans subsp. lubricantis RS1T, P. stutzeri ATCC 17588T, P. chengduensis CGMCC 2318T and P. xanthomarina KMM 1447T revealed 44.0%, 44.7%, 60.1%, 48.7%, 49.1%, 60.1%, 58.9% and 60.2% relatedness respectively. The predominant quinone system is ubiquinone-9 (Q-9). The major components of the cellular fatty acids (>10%) were summed feature 8 (comprising C18:1 ω7c /C18:1 ω6c), summed feature 3 (comprising C16:1 ω7c /C16:1 ω6c) and C16:0. The detected major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylcholine (PC). On the basis of phenotypic data, chemotaxonomic and phylogenetic properties, strain AL-54T can consider as a novel species within the genus Pseudomonas, for which the name Pseudomonas lopnurensis sp. nov. is proposed. The type strain is AL-54T (= JCM 19136T = CCTCC AB 2013066T = NRRL B-59987T).
Asunto(s)
Populus , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Fosfolípidos/análisis , Filogenia , Pseudomonas/genética , ARN Ribosómico 16S/genética , Ríos , Análisis de Secuencia de ADNRESUMEN
An endophytic bacterium, MA-69T, was isolated from the storage liquid in the stems of Populuseuphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain MA-69T was found to be short rod-shaped, Gram-stain-negative, non-spore-forming, aerobic and motile by means of a monopolar flagellum. According to phylogenetic analysis based on 16S rRNA gene sequences, strain MA-69T was assigned to the genus Pseudomonas with highest 16S rRNA gene sequence similarity of 97.5â% to Pseudomonas azotifigens JCM 12708T, followed by Pseudomonas matsuisoli JCM 30078T (97.5â%), Pseudomonas balearica DSM 6083T (97.1â%), Azotobacter salinestris ATCC 49674T (96.1â%) and Pseudomonas indica DSM 14015T (95.9â%). Analysis of strain MA-69T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the isolate to be distinctly delineated from species of the genus Pseudomonas. The DNA G+C content of strain MA-69T was 64.1 mol%. DNA-DNA hybridization with Pseudomonas azotifigens JCM 12708T, Pseudomonas matsuisoli JCM 30078T and Pseudomonas balearica DSM 6083T revealed 62.9, 60.1 and 49.0â% relatedness, respectively. The major fatty acids in strain MA-69T were summed feature 3 (25.7â%), summed feature 8 (24.0â%), C19â:â0cyclo ω8c (19.9â%), C16â:â0 (14.6â%) and C12â:â0 (6.3â%). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Q-9 was the major quinone in strain MA-69T. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain MA-69T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas tarimensis sp. nov. is proposed. The type strain is MA-69T (=CCTCC AB 2013065T=KCTC 42447T).
Asunto(s)
Filogenia , Tallos de la Planta/microbiología , Populus/microbiología , Pseudomonas/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Hibridación de Ácido Nucleico , Fosfolípidos/química , Pseudomonas/genética , Pseudomonas/aislamiento & purificación , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
A Gram-stain negative, aerobic, non-motile, short-rod-shaped bacterium, designated KBL-4-9T, was isolated from the storage liquid in the stems of Populus euphratica trees in Xinjiang, PR China. Strain KBL-4-9T grew at 4-45 °C (optimum 37 °C), 1-3â% (w/v) NaCl (optimum 1â%, w/v) and pH 5.5-9.5 (optimum pH 7.5). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KBL-4-9T belonged to the genus Pseudomonas and showed the highest 16S rRNA gene sequence similarity of 97.36â% to Pseudomonas pelagia CL-AP6T, followed by Pseudomonas xinjiangensis S3-3T (97.16â%), Pseudomonas sabulinigri J64T (97.15â%) and Pseudomonas xiamenensis C10-2T (96.47â%). Analysis of strain KBL-4-9T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the phylogenetic assignment of the isolates. The DNA G+C content was 61.6âmol% (sd = 2.19). DNA-DNA hybridization with P. pelagia CL-AP6T, P. xinjiangensis S3-3T and P. sabulinigri J64T revealed 49.3â% (sd = 3.04), 41.2â% and 52.5â% (sd = 4.45) relatedness, respectively. The major cellular fatty acids of strain KBL-4-9T were C16â:â0, C12â:â0, summed feature 8 (C18â:â1ω7c and/or C18â:â1ω6c) and C17â:â0 cyclo. The major isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain KBL-4-9T is considered to represents a novel species of the genus Pseudomonas, for which the name Pseudomonas populi sp. nov. is proposed. The type strain is KBL-4-9T ( = JCM 19138T = CCTCC AB 2013069T = NRRL B-59988T).
RESUMEN
An endophytic bacterium, designated K-38(T), was isolated from the storage liquid in the stems of Populus euphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain K-38(T) was found to be rod-shaped, Gram-stain-negative, aerobic, non-motile and non-spore-forming. Strain K-38(T) grew at temperatures of 25-37 °C (optimum, 28 °C), at pH 6.0-9.0 (optimum, pH 7.5) and in the presence of 0-3â% (w/v) NaCl with 1â% as the optimum concentration for growth. According to phylogenetic analysis based on 16S rRNA gene sequences, strain K-38(T) was assigned to the genus Rhizobium with highest 16S rRNA gene sequence similarity of 97.2â% to Rhizobium rosettiformans W3(T), followed by Rhizobium nepotum 39/7(T) (96.5â%) and Rhizobium borbori DN316(T) (96.2â%). Phylogenetic analysis of strain K-38(T) based on the protein coding genes recA, atpD and nifH confirmed (similarities were less than 90â%) it to be a representative of a distinctly delineated species of the genus Rhizobium. The DNA G+C content was determined to be 63.5 mol%. DNA-DNA relatedness between K-38(T) and R. rosettiformans W3(T) was 48.4â%, indicating genetic separation of strain K-38(T) from the latter strain. The major components of the cellular fatty acids in strain K-38(T) were revealed to be summed feature 8 (comprising C18â:â1ω7c and/or C18â:â1ω6c; 57.2â%), C16â:â0 (13.6â%) and summed feature 2 (comprising C12â:â0 aldehyde, C14â:â0 3-OH/iso-C16â:â1 I and/or unknown ECL 10.928; 11.0â%). Polar lipids of strain K-38(T) include phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids and two unidentified phospholipids. Q-10 was the major quinone in strain K-38(T). Based on phenotypic, chemotaxonomic and phylogenetic properties, strain K-38(T) represents a novel species of the genus Rhizobium, for which the name Rhizobium populi sp. nov. is proposed. The type strain is K-38(T) (â=âCCTCC AB 2013068(T)â=âNRRL B-59990(T)â=âJCM 19159(T)).