Metabolomic and biochemical disorders reveal the toxicity of environmental microplastics and benzo[a]pyrene in the marine polychaete Hediste diversicolor.

Recently, the abundance of environmental microplastics (MPs) has become a global paramount concern. Besides the danger of MPs for biota due to their tiny size, these minute particles may act as vectors of other pollutants. This study focused on evaluating the toxicity of environmentally relevant concentrations of MPs (10 and 50 mg/kg sediment) and benzo[a]pyrene (B[a]P, 1 µg/kg sediment), alone and in mixture, for 3 and 7 days in marine polychaete Hediste diversicolor, selected as a benthic bioindicator model. The exposure period was sufficient to confirm the bioaccumulation of both contaminants in seaworms, as well as the potential capacity of plastic particles to adsorb and vehiculate the B[a]P. Interestingly, increase of acidic mucus production was observed in seaworm tissues, indicative of a defense response. The activation of oxidative system pathways was demonstrated as a strategy to prevent lipid peroxidation. Furthermore, the comprehensive Nuclear Magnetic Resonance (NMR)-based metabolomics revealed significant disorders in amino acids metabolism, osmoregulatory process, energetic components, and oxidative stress related elements. Overall, these findings proved the possible synergic harmful effect of MPs and B[a]P even in small concentrations, which increases the concern about their long-term presence in marine ecosystems, and consequently their transfer and repercussions on marine fauna.

Microplastic characterization and assessment of removal efficiency in an urban and industrial wastewater treatment plant with submarine emission discharge.

Wastewater treatment plants (WWTPs) are significant contributors to microplastic (MP) pollution in marine ecosystems when they are inefficient. This study aimed to evaluate the effectiveness of microplastic removal from the effluent of the Anza WWTP (Morocco), which processes industrial and urban wastewater using a lamellar decantation system combined with a submarine emissary for treated water discharge. Additionally, this study investigated the presence of microplastics in the Atlantic seawater where treatment plant effluent is released. Microplastics were collected and extracted from wastewater and seawater samples to assess their abundance, shape, size, polymer type, and removal rates in the treatment plant. The findings revealed an average MP concentration of 1114 ± 90 MPs/L in the influent and 607 ± 101 MPs/L in the effluent, indicating a removal efficiency of 46 %. Seasonal analysis revealed the highest MP concentrations during the summer, with 2181.33 MPs/L in the influent and 1209 MPs/L in the effluent. Seawater samples from the discharge zone of the submarine emissary had an average MP concentration of 1600 MPs/m3. Characterization of the MPs revealed that fibers were the most common form of MPs in all the samples. The 500-100 µm size fraction was predominant in the WWTP samples, while MPs smaller than 1 mm were more abundant in the seawater samples. Seven polymer types were identified using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR), with PET, PE, PVC, PA, PS, PP, and EVA being the most prevalent. Scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM/EDX) revealed various degrees of weathering and chemical elements adhering to the MP surfaces. The results of this study provide valuable insights into the effectiveness of conventional treatment systems in removing microplastics and offer a reference for developing management strategies to mitigate MP pollution in Morocco's marine ecosystems.

Scenic degradation and visual pollution along the Agadir coastline (Morocco): Analysis and management.

Visual pollution refers to the degradation of landscape aesthetics, manifesting as visible deterioration. On the Agadir coast in Morocco, factors such as urbanization, erosion, marine wracking, litter, sewage, beach driving, and animal waste contribute to this issue, which detracts from coastal scenery. This study employs the coastal scenery evaluation system (CSES) to conduct a scenic assessment of 40 coastal sites, aiming to describe the current state of visual pollution and inform management interventions. The CSES utilizes a checklist comprising 18 physical and 8 human parameters to calculate a scenic evaluation index (D value), which categorizes coastal sites into five classes. These range from Class I - typically undisturbed natural areas with pristine scenic qualities - to Class V - which are degraded natural areas significantly impacted by human activities. The scenic evaluation classified these sites into three classes. Four sites (10 %) were classified as Class III, fifteen (37.5 %) as Class IV, and twenty-one (52.5 %) as Class V. No sites were classified as Classes I or II. The assessments presented here offer a comprehensive overview of the Agadir coastal scenery and establish a baseline for developing strategies to address visual pollution.

Unraveling the interplay between environmental microplastics and salinity stress on Mytilus galloprovincialis larval development: A holistic exploration.

The rise of plastic production has triggered a surge in plastic waste, overwhelming marine ecosystems with microplastics. The effects of climate change, notably changing salinity, have shaped the dynamics of coastal lagoons. Thus, understanding the combined impact of these phenomena on marine organisms becomes increasingly crucial. To address these knowledge gaps, we investigated for the first time the interactive effects of environmental microplastics (EMPs) and increased salinity on the early development of Mytilus galloprovincialis larvae. Morphological assessments using the larval embryotoxicity test revealed larval anomalies and developmental arrests induced by EMPs and increased salinity. Transcriptomic analyses targeting 12 genes involved in oxidative stress, apoptosis, DNA repair, shell formation, and stress proteins were conducted on D-larvae uncovered the potential effects of EMPs on shell biomineralization, highlighting the role of Histidine Rich Glycoproteine (HRG) and tubulin as crucial adaptive mechanisms in Mytilus sp. in response to environmental shifts. Furthermore, we explored oxidative stress and neurotoxicity using biochemical assays. Our findings revealed a potential interaction between EMPs and increased salinity, impacting multiple physiological processes in mussel larvae. Our data contribute to understanding the cumulative effects of emerging anthropogenic pollutants and environmental stressors, emphasizing the need for a holistic approach to assessing their impact on marine ecosystems.

Plastic pollution on Moroccan beaches: Toward baselines for large-scale assessment.

In Africa, Morocco is the 10th largest producer of plastic. The severity of this plastic has attracted increasing amounts of attention in the Moroccan Atlantic and Mediterranean in recent years. However, at the national level, there is limited knowledge of plastic pollution. To obtain an exhaustive and comprehensive evaluation of plastic pollution levels in Morocco, large-scale monitoring is needed on all the coasts of the country. In this context, this paper examined the composition, abundance, distribution, source and quality of beaches on two Moroccan coasts using four beach quality indices along 29 beaches. During two seasons, a total of 72,105 items were counted. The mean litter abundance was 0.31 items/m2, and the Mediterranean beaches were more dense than the Atlantic beaches. In particular, litter density was greater in spring (0.35 items/m2) than in summer (0.29 items/m2). The data indicate considerable differences in the density of marine debris according to the seasonality, beach typology and presence of rivers. Hazardous litter items were collected along both Moroccan coasts, constituting 8.41 % of the total collected items, with a mean of 0.026 items/m2. The use of environmental indices allowed us to classify Moroccan beaches as "moderate cleanliness", "moderate abundance" of plastics, "moderately safe" presence of hazardous litter and "mediocre" environmental status. The findings of the present study indicate that the sources of litter on both Moroccan coasts come mainly from recreational activities and dumping. The waste management practices recommended for Moroccan beaches include reducing sources, mitigating mitigation measures and changing littering behavior.

Exploring marine biofouling on anthropogenic litter in the Atlantic coastline of Morocco.

Today, the world is increasingly concerned about marine litter and its interaction with marine biodiversity. However, knowledge concerning the fouling organisms associated with marine litter is very limited in many of the world's marine environments. In this survey, we investigated biofouling on different types of marine litter washed up on all the coasts of the central Atlantic of Morocco. The findings revealed 21 fouling species belonging to 9 phyla (Arthropoda, Mollusca, Echinodermata, Annelida, Bryozoa, Porifera, Chlorophyta, Ochrophyta, and Ascomycota). More specifically, frequently observed fouling species include Mytilus galloprovincialis, Balanus laevis, Megabalanus coccopoma, and Pollicipes pollicipes species. Large marine litter items recorded the highest colonization of marine organisms in comparison to small ones. The frequency of occurrence (FO) of the species most commonly fouled on all coasts was Perforatus perforatus (FO = 48.60), followed by Mytilus galloprovincialis (FO = 45.80), Balanus trigonus (FO = 32.05), Balanus laevis (FO = 30.25), Megabalanus coccopoma (FO = 25.25), Bryozoa species (FO = 19.40), Spirobranchus triqueter (FO = 18.18), Lepas pectinata (FO = 14.45), and Pollicipes pollicipes (FO = 13.05). The majority of the species registered in this study are sessile. Substrate coverage by fouling taxa was significantly different between plastic substrate and other types of marine litter. Likewise, this study revealed that the proportion of fouling organisms is higher on rough surfaces. Overall, this research could be crucial to understanding the little-known subject of marine litter and its colonization by marine biota. Given that these marine litters can act as vectors and cause ecological, biogeographical, and conservation issues in the marine environment, minimizing the quantity of anthropogenic litter reaching the Moroccan Atlantic could significantly reduce its accumulation on the sea surface and seabed, thereby reducing the risk of invasion by non-indigenous species.

Examining the influence of COVID-19 lockdowns on coastal water quality: A study on fecal bacteria levels in Moroccan seawaters.

Fecal bacteria in bathing seawater pose a substantial public health risk and require rigorous monitoring. The unexpected beach closures during the COVID-19 lockdowns have afforded unique opportunities to evaluate the impact of human activities on bathing water quality (BWQ). This study examined the temporal changes in fecal coliforms (FC) and streptococci (FS) within bathing seawater across a popular coastal region in Morocco during two lockdown periods (2020 L and 2021 L), comparing these data with observations from pre-lockdown years (2018, 2019) and post-lockdown periods (2020, 2021, 2022). Our findings illuminate the influential role the hiatus periods played in enhancing bathing water quality, attaining an "excellent" status with marked reductions in fecal coliform and streptococci levels. Consequently, the FC/FS analysis exposed a clear preponderance of humans as the primary sources of fecal contamination, a trend that aligns with the burgeoning coastal tourism and the escalating numbers of beach visitors. Additionally, the presence of domestic animals like camels and horses used for tourist rides, coupled with an increase in wild animals such as dogs during the lockdown periods, compounded the potential sources of fecal bacteria in the study area. Furthermore, occasional sewage discharge from tourist accommodations and wastewater treatment plants may also contribute to fecal contamination. To effectively mitigate these concerns and bolster public health, a commitment to relentless surveillance efforts, leveraging novel and innovative tools, is essential. These findings underline the crucial interplay between human activities and the health of our coastal ecosystems, emphasizing the need for sustainable practices for a safer and healthier future.

Comparative study of pristine and polluted estuaries in Souss Massa National Park (Morocco): Implications for conservation strategies.

Estuaries are among the ecosystems most affected by human actions worldwide. Economic development in Morocco puts pressure on these aquatic systems, making them vulnerable. This study compares the state of benthic communities between a pristine estuary (Massa estuary) and a polluted one (Souss estuary). Both ecosystems belong to the Souss Massa National Park (SMNP), registered in the Ramsar list for its ecological importance as a Marine Protected Area (MPA). Twenty-one benthic species were identified in the pristine estuary, but only six species in the polluted one. Similar differences were detected for the species abundance and biomass. The total organic matter and the water-dissolved oxygen also revealed a notable negative effect of the sewage discharge. The results confirmed human disturbances on faunal communities related to direct wastewater inputs and indirect anthropogenic activities such as the urbanization and the litter generation. A combination of ending wastewater discharge and adding tertiary-level water treatment plants is recommended. The findings highlight the importance of MPAs in conservation strategies if coupled with continuous surveillance of pollution.

The halophyte Cakile maritima Scop. 1772 as a trap of plastic litter on the Moroccan coast.

Some plant communities of coastal dunes may affect the magnitude and distribution of litter on the ecosystem. In this study, the aim is to assess the aptitude of the halophyte Cakile maritima Scop. 1772 to be a trap and sink of plastic litter on the Moroccan Atlantic coast. Overall, a significant difference was noted between plastic litter trapped in C. maritima patches (1173 items) and control plots (502 items). Food containers and ropes were the most common trapped items. Shoreline and recreational activities, followed by dumping and ocean/waterway activities are the main sources of the trapped plastic items. The findings suggest the expansion of the cleaning operations to include coastal dunes, the need to change behavior among beachgoers in regard to food plastics disposal, as well the control of C. maritima distribution in the study area, and similar plant species in other regions.

Binational survey using Mytilus galloprovincialis as a bioindicator of microplastic pollution: Insights into chemical analysis and potential risk on humans.

Microplastic (MP) contamination in edible mussels has raised concerns due to their potential risk to human health. Aiming to provide valuable insights regarding the occurrence, physicochemical characteristics, and human health implications of MP contamination, in the present study, two nationwide surveys of MP contamination in mussels (Mytilus galloprovincialis) were conducted in Morocco and Tunisia. The results indicated that MP frequency ranged from 79 % to 100 % in all the analyzed samples. The highest MP density was detected in mussels from Morocco (gills "GI": 1.88 MPs/g ww-1; digestive glands "DG": 0.92 MPs/g ww-1) compared to mussels of Tunisia (GI: 1.47 MPs g- 1; DG: 0.79 MPs g- 1). No significant differences in MP density were found between the two organs (GI and DG) for both countries. MPs were predominantly blue and black fibers, and smaller than 1000 µm. Seven polymeric types were identified, of which PET, PP, and PE were the most abundant, accounting for >87 % of all samples. Scanning Electron Microscopy (SEM) coupled with Energy dispersive X-ray (EDX) showed that most MPs have noticeable signs of weathering and inorganic components on their surface. The highest MP daily intake was found in children, while the lowest was estimated in women and men. Moreover, the annual dietary exposure of MPs through mussel consumption was estimated to be 1262.17 MPs/year in Morocco and 78.18 MPs/year in Tunisia. The potential risk assessment of MPs in mussels based on the polymer hazard index (PHI) was estimated in the high-risk levels, implying that MPs may pose health risks to humans. Overall, this research suggests that the consumption of mussels represents a considerable MP exposure route for the Moroccan and Tunisian populations.

Trace metals in urbanized coasts: The central Atlantic of Morocco as a case study.

This study describes the contamination, accumulation, and ecological risk assessment of five trace metals (Cd, Pb, Cu, Zn, and Cr) in sediments of an urbanized beach in the central Atlantic coastline of Morocco. The two-year investigation (2018 and 2019) included six sampling sites along a 6 km coastal reach. In both years, none of the studied trace metals exceeded the background or the sediment quality guidelines (SQGs). The eco-toxicological indices revealed low degree of contamination, unpolluted ecosystem, and low ecological risk of metals. However, Cd exceeded the background value and some SQGs following the increase of anthropogenic activities in 2019. Likewise, it indicated unpolluted to moderately polluted sediment as well as moderate ecological risk. Overall, it is highly recommended to mitigate the avoidable anthropogenic activities (marine litter generation, sewage discharge, intense urbanization, and vehicle traffic on the beach) that lead to the elevation of metal pollution in the study area.

Occurrence, characteristics, and removal of microplastics in wastewater treatment plants located on the Moroccan Atlantic: The case of Agadir metropolis.

Wastewater treatment plants (WWTPs) are some of the main sources of microplastics (MPs) in the environment. However, studies on the occurrence and removal efficiency of MPs in WWTPs are still scarce, especially in African countries. Thus, the aim of this work was to study the abundance of MPs in the influent and effluent of two WWTPs (Aourir and M'zar) from the Agadir metropolis (Moroccan Atlantic). The two WWTPs receive different wastewater inputs (domestic and industrial). In addition, the impacts of seasonality on the fate and removal efficiency were investigated. The results showed that the MPs abundance in the wastewater decreased from 188 MPs/L in the influent to 50 MPs/L in the effluent for Aourir WWTP (domestic inputs); while the abundance was greater in the M'zar WWTP (urban and industrial inputs) recording a mean value of 519 MPs/L and 86 MPs/L in the influent and effluent, respectively. MPs collected in the Aourir WWTP ranged from 290 to 3200 µm, while MPs from the M'zar WWTP, ranged from 330 to 4200 µm. Overall, the size range of 100-500 µm was the most abundant for both WWTPs. Fibers were the highest MPs morphotype found, followed by fragments. MP colors were mainly red, black, blue, and transparent for both treatment plants. Additionally, FTIR spectroscopy showed the presence of eight different polymers, mainly polyethylene (PE), polypropylene (PP), and polystyrene (PS). Seasonal variation analysis showed that MPs abundance in summer was significantly higher compared to other seasons. However, the comparison of the removal efficiency (RE) between the different seasons indicated that the winter season (74 %) recorded the highest RE for Aourir WWTP. Conversely, spring (87 %) recorded the highest RE for M'zar WWTP. SEM/EDX micrographs showed different degrees of weathering and chemical elements adhering to the surface of the MPs. The findings of the current study will serve as a baseline for future considerations about management strategies, wastewater reuse, as well as the understanding of the occurrence of microplastic pollution along the marine ecosystems of Morocco.

Has the "Covid-19" lockdown an impact on beach faunal communities? The central Atlantic coast of Morocco as a case study.

The restrictions related to the COVID-19 pandemic have led to a global hiatus in anthropogenic activities; several scientists have utilized this unique opportunity to assess the human impact on biological systems. In this study, the study describes for a period of five years (2018-2022) how the faunal community have been affected by human disturbances, as well as the effect of the "anthropause" period driven by the COVID-19 lockdown. The results confirmed human disturbances on faunal communities related to coastal urbanization. It was found that the "anthropause" period showed the highest values of abundance and biomass, hence the "COVID-19 lockdown" allowed recovery of faunal communities. The findings highlight the impact of human disturbances and that the community showed resilience. Overall, the authorities must perform restrictive measures aiming to mitigate the impact of anthropogenic activities in the study area including the banning of off-road and recreational vehicles, carrying out efficient cleaning and grooming operations, monitoring the severe harvesting of edible species, as well as penalizing the disposal of anthropogenic waste and sewage discharge from the touristic facilities. Likewise, management actions such as the temporal beach closures and the regular surveillance could be advantageous to provide a more sustainable exploitation of sandy beaches.

Floating microplastics pollution in the Central Atlantic Ocean of Morocco: Insights into the occurrence, characterization, and fate.

This work presents preliminary results about abundance, distribution, characteristics, sources, and fate of microplastics (MPs) in the Central Atlantic Ocean (CAO) of Morocco. The investigation was conducted into three subsections, each characterized by different types of human activities and covering rural, village, and urban areas. MPs were detected in 100 % of the sampling sites. The abundances varied from 0.048 to 3.305 items/m3, with a mean abundance of 0.987 ± 1.081 items/m3. MPs abundance was higher in surface seawater linked to urban areas compared to village and rural areas. The dominant polymer type was polyester (PET-53.8 %) followed by polypropylene (PP-24.36 %), polyamide (PA-7.56 %), polystyrene (PS-6.88 %), polyvinyl chloride (PVC-2.64 %), ethylene vinyl acetate (EVA-2.60 %), polyetherurethane (PUR-1.36 %), and acrylic (AC-0.8 %). Fibers were the most dominant shapes accounting for over 50 %. MPs were mainly smaller than 2 mm in size (71 %) and characterized by colorful aspects. These findings suggested that wastewater treatment plant (WWTP) effluents and anthropogenic activities (industry, tourism, sanitation, and fishing) are the major pollution sources of MPs in the study area. SEM/EDX micrographs showed different weathering degrees and chemical elements adhered to the MPs surface.

Microplastics pollution in sediments of Moroccan urban beaches: The Taghazout coast as a case study.

This work describes the spatiotemporal analysis of microplastics (MPs) pollution in sediments collected on a high urbanized beach located in Taghazout coast, central Atlantic part of Morocco. The study area is mainly composed of moderately well-sorted fine sands with an average density of MPs that ranged between 915 MPs/kg in 2018 and 1448 MPs/kg in 2019. The most polluted sites were in the south part of Taghazout coast, close to facilities of where beachgoers are often found. Microplastic Pollution Index (MPPI), Microplastic Impact Coefficient (CMPI), Agglomerative Hierarchical Clustering (AHC), and Multidimensional Scaling (MDS) revealed spatiotemporal variation of MPs pollution. Thus, the principal component analysis (PCA) showed a low correlation between the sediment characteristics (i.e., grain size, sorting, skewness) and MPs densities. Overall, the outputs of this baseline recommend implementing plastic management strategies to eliminate or at least minimize the collateral effects generated by MPs pollution in sediments of urbanized beaches.

The wedge clam Donax trunculus L., 1758 as a bioindicator of microplastic pollution.

This study describes the use of D. trunculus as a bioindicator of microplastic (MPs) pollution due to beach urbanization related to the development of the Taghazout Bay tourist resort in Morocco. In six sites located on the coast, MPs densities ranged from 1.75 to 5.93 items per gram of D. trunculus wet weight. MPs contamination was significantly influenced spatiotemporally by the urbanization degree in the area. The shapes found were fibers (90.5%) and fragments (9.5%). The Fourier Transform Infrared Spectroscopy (FTIR) analysis reported two polymers: polyethylene (65%) and polypropylene (35%). MPs colors were dark and light, with a predominance of light blue (23.8%). The species ingested different sizes of MPs, mainly the 0.1 and 0.5 mm fractions. Scanning Electron Microscope coupled with an energy dispersive X-ray analyzer (SEM/EDX) revealed weathered MPs with chemical elements adhered to their surface (Cu, Fe, Mg, S, Cl, etc.). The results of this study recommend the use of D. trunculus as a new bioindicator of MPs pollution following an urban change in the coastal scenery. Also, a sustainable coastal management must be implemented in the study area.

Microplastics pollution along the central Atlantic coastline of Morocco.

This baseline study describes the microplastic (MPs) problem on seven beaches located on Agadir, central Atlantic coast of Morocco. Microplastics abundances (densities) ranged from 7680 MPs/kg to 34,200 MPs/kg above other world beaches. The following shapes were found: fibers, fragments, films, and pellets. Fibers were the dominant typology with a 73%. Polyethylene (PE) was the predominant polymer in all beaches with an average percentage of 59%, followed by Polypropylene (PP - 18%), Polystyrene (PS - 9%), Polyvinyl chloride (PVC - 8%), and Ethylene-vinyl acetate (EVA - 6%). Microplastics abundances presented in this work suggest continuous inputs of plastics of all sizes and types. Also, all observed shapes highlight a combination of sources (primary and secondary). Central Atlantic Moroccan beaches are currently affected by extreme values of MPs that demand urgent interventions to restore environmental quality.

Personal protective equipment (PPE) pollution associated with the COVID-19 pandemic along the coastline of Agadir, Morocco.

The increasing use of personal protective equipment (PPE) as a sanitary measure against the new coronavirus (SARS-CoV-2) has become a significant source of many environmental risks. The majority of the governments enforce the use of PPE in public areas, such as beaches. Thus, the use and disposal of PPE have compromised most solid waste management strategies, ultimately leading to the occurrence of PPE polluting the marine environment. The present study aimed to monitor the PPE pollution associated with COVID-19 along the coastline of Agadir, Morocco. In parallel, the influence of the activities carried out in each sampled beach before and after the lockdown break was reported. Overall, a total number of 689 PPE items were identified, with a mean density of 1.13 × 10-5 PPE m-2 (0-1.21 × 10-4 PPE m-2). The majority of the PPE items found were face masks (96.81%), out of which 98.4% were surgical masks and 1.6% were reusable cloth masks. The most polluted sites were the beaches with recreational activities, followed by surfing, and fishing as the main activity. Importantly, PPE density increased significantly after lockdown measures. Additionally, the discarded PPE sampled in the supralittoral zone was higher than PPE recorded in the intertidal zone. This confirms that PPE items are driven by the beachgoers during their visit. PPE items are a source of microplastic and chemical pollutants, a substrate to invasive species colonization, and a potential threat of entanglement, ingestion, and/or infection among apex predators. In the specific case of Agadir beaches, significant efforts are required to work on the lack of environmental awareness and education. It is recommended to improve beach cleaning strategies and to penalize incorrect PPE disposal. Additional alternatives may be adopted, as the involvement of biodegradable materials in PPE manufacturing, recycling through pyrolysis, and encouraging reusable and washable masks.