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PLoS One ; 14(12): e0221540, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31790419

RESUMEN

Ultraviolet radiation (UVR) is widely known as deleterious for many organisms since it can cause damage to biomolecules either directly or indirectly via the formation of reactive oxygen species. The goal of this study was to analyze the capacity of high-mountain Espeletia hartwegiana plant phyllosphere microorganisms to survive UVR and to identify genes related to resistance strategies. A strain of Deinococcus swuensis showed a high survival rate of up to 60% after UVR treatment at 800J/m2 and was used for differential expression analysis using RNA-seq after exposing cells to 400J/m2 of UVR (with >95% survival rate). Differentially expressed genes were identified using the R-Bioconductor package NOISeq and compared with other reported resistance strategies reported for this genus. Genes identified as being overexpressed included transcriptional regulators and genes involved in protection against damage by UVR. Non-coding (nc)RNAs were also differentially expressed, some of which have not been previously implicated. This study characterized the immediate radiation response of D. swuensis and indicates the involvement of ncRNAs in the adaptation to extreme environmental conditions.


Asunto(s)
Deinococcus/fisiología , Deinococcus/efectos de la radiación , Ecosistema , Tolerancia a Radiación , Rayos Ultravioleta , Adaptación Fisiológica/efectos de la radiación , Deinococcus/genética , Deinococcus/aislamiento & purificación , Regulación Bacteriana de la Expresión Génica/efectos de la radiación , ARN Bacteriano/genética , Análisis de Supervivencia
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