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Lett Appl Microbiol ; 36(4): 227-9, 2003.
Artículo en Inglés | MEDLINE | ID: mdl-12641716

RESUMEN

AIMS: To investigate properties of the proteolytic activity of a yeast cell wall lytic soil bacterium identified as an Arthrobacter species. METHODS AND RESULTS: The organism was grown at pH 7.5 and 30 degrees C in shake flasks on media with different complex subtrates. Highest proteolytic activity assayed with azocaseine was detected in media with wheat gluten. In addition, l-leucine, l-alanine exopeptidase activity and esterase activity were found. The proteolytic activity showed stability up to pH 12, with a maximum at pH 11. The temperature optimum was at 55 degrees C, but there was a loss in enzyme activity of 50% within 2 h. The proteolytic activity was inhibited by 3,4-dichloroisocumarin, whereas there was little or no effect with EDTA, pepstatin A or E64. CONCLUSIONS: The proteolytic activity is highly alkaline stable. The formation of the enzyme can be induced by media with high protein content.


Asunto(s)
Arthrobacter/metabolismo , Arthrobacter/crecimiento & desarrollo , Caseínas/metabolismo , Pared Celular/metabolismo , Medios de Cultivo , Endopeptidasas/metabolismo , Concentración de Iones de Hidrógeno , Metaloendopeptidasas/metabolismo , Saccharomyces cerevisiae/metabolismo , Microbiología del Suelo , Especificidad por Sustrato , Temperatura
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