Predicting the dynamics of per- and polyfluoroalkyl substances in coastal regions of Africa: vulnerability index and adverse ecological pathways from remote-sensed variables.

This study aimed to predict the dynamics of per- and polyfluoroalkyl substance (PFAS) contamination and ecological vulnerability within coastal regions of Africa utilizing time-averaged remote-sensed data patterns from 2020 to 2023. The analysis identified PFAS contamination hotspots along the coast of Africa, particularly in western Africa around Nigeria and in areas spanning Equatorial Guinea and Guinea-Bissau, with risk influenced by eastward wind patterns, overland runoff, and elevated aerosol optical depth (AOD) values. Regional trends indicated that variations in solar energy absorption and surface air temperature could influence PFAS dynamics in North Africa, South Africa, East Africa, and West Africa. In North Africa, intermediate overland runoff and lower sea-surface temperatures were observed. In South Africa, there were intermediate runoff levels and warmer sea-surface temperatures. East Africa experienced intermediate runoff as well. In West Africa, there was increased susceptibility to high overland runoff and aerosol-related PFAS contamination. From the weighted vulnerability index, significant disparities in environmental conditions across African coastal regions revealed that North Africa had relatively lower vulnerability, while West Africa had the highest susceptibility to per- and polyfluoroalkyl substance (PFAS) contamination. This study emphasizes the necessity for region-specific vulnerability index models and targeted mitigation strategies to address diverse ecological and health risks from PFAS contamination along the African coast. Regional and international collaboration, spearheaded by organizations such as the AU and ECOWAS, is essential, with tailored policies aligned with the SDGs, Agenda 2063, and NEPAD crucial for effective environmental management, urging policymakers to prioritize cooperation and resource sharing for comprehensive sustainability goals.

Urbanization effects on growth and otolith asymmetry in Chrysichthys nigrodigitatus and Oreochromis niloticus within tropical coastal lagoon watersheds.

In this study, we investigated the growth dynamics and otolith shape asymmetry of two fish species, Chrysichthys nigrodigitatus (CN) and Oreochromis niloticus (ON), within urbanized watersheds of the southern lagoon system, Nigeria. Using the von Bertalanffy growth model (VBGM), in addition to sediment metal concentration indices such as the average shale content, index of geoaccumulation (Igeo), contamination factor (CF), pollution load index (PLI), and potential ecological risk (PER) index, contamination levels were classified, and ecological risks were assessed. Notably, a lower growth potential (t0) was observed in CN at Ikorodu than at Epe, with similar trends for ON in the Epe during the dry season. Otolith asymmetry patterns, particularly in the CN at Ikorodu and ON in the Epe during the dry season, exhibited distinct ecological variations, indicating heightened stress levels at Ikorodu. Sediment analyses revealed moderate to strong contamination (Cd, Pb, Ni, and Cr) in both Lagos Lagoon (Ikorodu) and Epe Lagoon, with Ikorodu exhibiting notably high to moderate contamination levels according to the CF index. Elevated PLI values for Cd and Pb in Ikorodu, in addition to greater PER, indicated increased risk, with Cd posing a high risk (61.42%) and Pb posing a moderate risk (49.50%). Additionally, the reduced asymptotic length in the Epe during the dry season suggests that Chrysichthys nigrodigitatus is adaptable to seasonal variations, while divergent growth patterns in both areas indicate the existence of trade-off mechanisms in response to changing conditions. Habitat-specific otolith asymmetry and metal contamination underscore species adaptability, with wider stressor variability in Lagos than in Epe. Furthermore, multidimensional scaling analysis highlights the intricate relationship between otolith shape variables and environmental factors, emphasizing the need for tailored conservation efforts in urbanized watersheds.

Role of niche and micro-habitat preferences in per- and polyfluoroalkyl substances occurrence in the gills of tropical lake fish species.

The present study has investigated per- and poly-fluoroalkyl substances (PFAS) in the gill tissues of various fish species inhabiting different trophic levels within Eleyele Lake, a tropical freshwater lake in Nigeria. The mean concentrations of PFAS congeners were determined, and their trends and patterns were analyzed across different trophic species. The results revealed variations in congener abundance and species-specific patterns that was influenced by habitat and niche preferences. Multivariate associations using canonical-correlation analysis (CCA) revealed distinct trends in the relationships between gill concentrations of specific PFAS congeners and different trophic groups. The strongest congener relationships were observed in the pelagic omnivore (Oreochromic niloticus: ON) with positive associations for 4:2 FTS, 9CL-PF3ONS, PFTDA, MeFOSA and PFHxS. The differences in congener profiles for the two herbivorous fish (Sarotherodon melanotheron (SM) and Coptodon galilaeus (CG)) reflect possible divergence in microhabitat and niche preferences. Furthermore, the congener overlaps between the herbivore (CG), and benthic omnivore (Clarias gariepinus: ClG) indicate a possible niche and microhabitat overlap. Our study provides valuable insights into the congener dynamics of PFAS at Eleyele Lake. However, the dissimilarity and overlapping PFAS congener profile in fish gills reflects the interplay of species niche preference and microhabitat associations. The present study highlights the need for further research to assess ecological risks and develop effective PFAS management strategies.

Gonad pathology, sex hormone modulation and vitellogenin expression in Chrysichthys nigrodigitatus from Lagos and Epe lagoons within the southern-lagoon system, Nigeria.

Introduction: Estrogenic chemicals in aquatic environments impact fish reproductive health, with vitellogenin protein levels serving as a crucial biomarker for xenoestrogen exposure. Limited knowledge exists on estrogenic effects in tropical environments, prompting an investigation into the influence of environmental estrogens on Chrysichthys nigrodigitatus in Lagos and Epe lagoons. Methods: A total of 195 fish samples underwent analysis for vitellogenin protein, sex hormones (testosterone and 17 ß-estradiol), and gonad pathology in effluent-receiving areas of the specified lagoons. Results: Gonadal alterations were observed in male and female fish, including empty seminiferous tubules and distorted ovaries. Intersex occurred in 3.81% of Lagos and 3.33% of Epe. Testosterone levels were generally higher in females and males from both lagoons, while E2 levels were higher in females from both lagoons, with Lagos showing higher levels than Epe. Vtg levels were higher in males than females in Lagos samples but showed no significant difference in Epe samples. Discussion: Contaminant analysis revealed similar trends in metals (Hg, As, Cr) and phthalates (DEHP, DBP, DEP) in both sexes in the Epe population. Multivariate depictions from the PCA showed sex-specific patterns of metal uptake (Cd) in male fishes at the Lagos Lagoon. The positive association between higher pH loadings and metal and DBP levels in sediment at the Lagos lagoon suggests the influence of higher alkalinity in lower bioavailability of contaminants. Conclusion: Endocrine disrupting effects were observed in male and female Chrysichthys nigrodigitatus in Lagos and Epe lagoons populations, with notable differences in hormone and contaminant concentrations between the two lagoon systems. Identification of specific contaminants and their spatial and temporal trends can inform targeted management and remediation efforts to protect and restore these valuable aquatic ecosystems.

Occurrence, bioaccumulation and trophic dynamics of per- and polyfluoroalkyl substances in two tropical freshwater lakes.

We have investigated the occurrence, distribution, and biomagnification of per- and polyfluoroalkyl substances (PFAS) in two tropical lakes (Asejire and Eleyele) of Southwestern Nigeria, with contrasting urban intensities. Over an 8-month period, we sampled sediment and fish species (Clarias gariepinus: CIG; Oreochromis niloticus: ON; Coptodon guineensis: CG; Sarotherodon melanotheron: SM) across trophic levels, and analyzed various PFAS congeners, in addition to a select group of toxicological responses. While herbivores (SM) and benthic omnivores (CIG) at Asejire exhibited elevated levels of PFBS and PFOS, the pelagic omnivores (ON) showed a dominance of PFOS, PFDA, PFHxDA and EtFOSE in the muscle. At the Eleyele urban lake, PFAS patterns was dominated by PFBS, EtFOSE, PFPeS, PFOcDA and PFOS in the herbivores (SM, CG), EtFOSE, PFOS and PFBS in the pelagic omnivore (ON) and benthic omnivore (ClG). The estimated biomagnification factor (BMF) analysis for both lakes indicated trophic level increase of PFOS, PFUnA and PFDA at the suburban lake, while PFOS and EtFOSE biomagnified at the urban lake. We detected the occurrence of diSAMPAP and 9CL-PF3ONS, novel compounds not commonly reported, in PFAS studies at both lakes. The studied toxicological responses varied across trophic groups in both lakes with probable modulations by environmental conditions, trophic structure, and relative PFAS exposures in the lakes. The present study documents, for the first time in Nigeria, or any other African country, the role of urbanization on contaminant load into the environment and their implications for contaminant dynamics within the ecosystem and for aquatic food safety.

Habitat sensitivity in the West African coastal area: inferences and implications for regional adaptations to climate change and ocean acidification.

This study focuses on assessing coastal vulnerability and habitat sensitivity along the West African coast by delineating hotspots based on surface temperature, pH, chlorophyll-a, particulate organic carbon, and carbonate concentrations between 2018 and 2023 depending on data availability. Initial exploration of these variables revealed two distinct focal points i.e., the Togo-Nigerian coastal stretch and the stretch from Sierra Leone to Mauritania. Lower pH trends (acidification) in surface waters were observed off the West African coast, particularly in areas around the south-south Niger Delta in Nigeria and the coastal regions of Guinea and Guinea Bissau. Sea surface temperature analysis revealed highest temperatures (27-30°C) within Nigeria to Guinea coastal stretch, intermediate temperatures (24-27°C) within the Guinea Bissau and Senegal coastal stretch, and the lowest temperatures off the coast of Mauritania. Furthermore, correlation analysis between sea surface temperature and calcite concentration in the Mauritania-Senegal hotspot, as well as between overland runoff and particulate organic carbon in the Togo-Nigeria hotspot, revealed strong positive associations (r>0.60) and considerable predictive variability (R2 ≈ 0.40). From the habitat sensitivity analysis, certain regions, including Cape Verde, Côte d'Ivoire, Nigeria, Senegal, and Sierra Leone, exhibited high sensitivity due to environmental challenges and strong human dependence on coastal resources. Conversely, Gambia, Guinea, Guinea-Bissau, Liberia, and Togo displayed lower sensitivity, influenced by geographical-related factors (e.g. coastal layout, topography, etc.) and current levels of economic development (relatively lower industrialization levels). Regional pH variations in West African coastal waters have profound implications for ecosystems, fisheries, and communities. Addressing these challenges requires collaborative regional policies to safeguard shared marine resources. These findings underscore the link between ecosystem health, socioeconomics, and the need for integrated coastal management and ongoing research to support effective conservation.

Redox-active metals and oxidative stress-mediated myopathies in Callinectes amnicola, blue crab populations from impacted sites of the Lagos Lagoon: inferences for adverse ecological outcomes.

While oxidative stress pathways are associated with a wide variety of tissue pathologies, its applications for evaluating and discerning ecological risks are limited. This study seeks to associate trends of lipid peroxidation and oxidative stress to risks of muscle pathologies in blue crabs inhabiting regions of the Lagos Lagoon. Crab samples (n = 520) were selected from pollution-impacted sites of the lagoon at Iddo, Ajah, Okobaba, Makoko, and the mid-lagoon area (control site). Antioxidant enzyme capacity, i.e., superoxide dismutase, catalase, glutathione peroxidase (GPx), and glutathione-S-transferase, and lipid peroxidation were evaluated in the muscle tissue of the blue crabs. The study findings showed distinct patterns of metal uptake in muscle, with redox-active metals (Cu and Zn) and redox-inactive metals (Pb and Cd) exhibiting site-specific differences. Additionally, there were changes in antioxidant modulation, lipid peroxidation, and the presence of associated myopathies. Blue crabs from sites (Makoko and Ajah) with greater uptake of redox-active metals (Cu and Zn) in muscle tissue showed higher trends of lipid peroxidation and the most prevalence of severe regression-type myopathies. Sites with lower uptake of redox-active metals showed the predominance of circulatory-type myopathies. This study also provides evidence of severe necrosis and myositis associated with digenean parasite cysts in crab muscle. Pathological evidence of severe skeletal muscle deterioration in the presence of greater lipid peroxidation could have implications for motor-neuron activity and reduced force-generating capacity necessary for adaptive responses in the wild. We conclude that elevated uptake of redox metals could aggravate the onset of myopathies in wild populations.

Public health implications of endocrine disrupting chemicals in drinking water and aquatic food resources in Nigeria: A state-of-the-science review.

This state-of-the-science review is aimed at identifying the sources, occurrence, and concentrations of EDCs, including potential public health risks associated with drinking water and aquatic food resources from Nigerian inland waters. A total of 6024 articles from scientific databases (PubMed, Scopus, Web of science, ScienceDirect, Google Scholar, and African Journals Online) were identified, out of which, 103 eligible articles were selected for this study. Eleven (11) classes of EDCs (OCPs, PCBs, PBDEs, PAHs, BPA, OTs, PEs, PCs, PPCPs, sterols and n-alkanes) were identified from drinking waters, river sediments and aquatic food species from Nigerian rivers, showing that OCPs were the most studied and reported EDCs. Analytical methods used were HPLC, LC-MS/MS, GC-FID, GC-ECD and GC-MS with all EDCs identified to originate from anthropogenic sources. Carcinogenic, mutagenic, and teratogenic effects were the highest (54.4 %) toxicological effects identified, while reproductive/endocrine disruptive effects (15.2 %) and obesogenic effects (4.3 %) were the least identified toxicological effects. The targeted hazard quotient (THQ) and cancer risk (CR) were generally highest in children, compared to the adult populations, indicating age-specific toxicity. PEs produced the highest THQ (330.3) and CR (1.2) for all the EDCs in drinking water for the children population, suggesting enhanced vulnerability of this population group, compared to the adult population. Due to associated public health, wildlife and environmental risk of EDCs and their increasing concentrations in drinking water and food fish species from Nigerian inland waters, there is an urgent need for focused and strategic interventions, sensitization and policy formulation/implementation towards public health and aquatic food safety in Nigeria.

Detection and occurrence of microplastics in the stomach of commercial fish species from a municipal water supply lake in southwestern Nigeria.

Microplastics (MPs) are physical anthropogenic pollutants and their ability to act as contaminant vectors in biological matrices is of serious ecosystem and human health concern. In the present study, we have, for the first time, screened and detected MPs in the stomach of a select group of commonly consumed fish species from a municipal water supply lake (Eleyele) in Nigeria. A total of 109 fish samples consisting of eight (8) species: Coptodon zillii (CZ: n = 38), Oreochromis niloticus (ON: n = 43), Sarotheron melanotheron (SM: n = 19), Chrysicthys nigrodigitatus (CN: n = 3), Lates niloticus (LN: n = 3), Paranchanna obscura (PO: n = 1), Hemichromis fasiatus (HF: n = 1), and Hepsetus odoe (HO: n = 1) were collected between February-April, 2018. Fish stomach content was screened for the presence of MPs using the density gradient separation technique (NaCl hypersaline solution) and examined using a fluorescence microscope. MPs were present in all the species screened (except H. fasciatus) with a frequency of 69.7% positive individuals in the examined species. MP prevalence was highest in ON (34%) > CZ (32%) > SM (13%) > CN (6%) and 5% each, for PO HO, and LN. On average, 1-6 MPs with sizes ranging between 124 µm and 1.53 mm were detected per individual. However, the highest number (34) of MPs was detected in the stomach of SM. Principal coordinate analysis (PCA) identified ecological variables such as habitat, feeding mode, and trophic levels as critical factors that may determine and influence MP uptake in fish population. The PCA showed stronger association between fish habitat, feeding mode, and trophic level with MP size and number in the benthopelagic species (ON CZ and SM), compared to demersal species (PO CN HO and LN). Given that MPs can act as vectors for the transfer of pathogens and environmental contaminants (both legacy and emerging), in addition to direct health risks to aquatic organisms, our findings raise concerns on the potential human/wildlife health effects of MPs in these economically and ecologically important food fishes.

Quality screening of the Lagos lagoon sediment by assessing the cytotoxicity and toxicological responses of rat hepatoma H4IIE and fish PLHC-1 cell-lines using different extraction approaches.

In this study, sediment samples from Makoko and Ikorodu sites of the Lagos lagoon (Nigeria) were screened for toxicological responses on mammalian and fish cell lines using different extraction methods. Rat hepatoma H4IIE and fish PLHC-1 cell-lines were exposed to serial dilutions of the elutriate, polar and non-polar extracts. We evaluated exposed cells for cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity. Cells exposed to polar and water extracts from Makoko and Ikorodu showed viability percentage of >80% at 48 h. On the other hand, exposure to the non-polar extracts exhibited cell viability of 50-60% at all tested dilutions. For both cell lines, a significant concentration-dependent induction of cyp1a mRNA was observed after exposure to the different extracts from both sites. Interestingly, the extracts affected functional enzymes differently for both cell lines. For H4IIE cells, while EROD activity paralleled cyp1a mRNA expression patterns, MROD showed significant concentration-specific reduction in cells exposed to polar and water extracts. On the contrary, while the MROD activity paralleled cyp1a mRNA, EROD activity was significantly inhibited in PLHC-1 cells exposed to water-, polar and non-polar extracts from both sites. These observations paralleled sediments PAH contamination burden from the study sites as revealed by co-relation analysis. In conclusion, although the different extracts did not exert high cytotoxic effects (except the non-polar) at the tested concentrations, they significantly modulated phase I biotransformation responses, showing that the studied sediments contain complex chemical mixture in the different extracts, with potential for overt physiological and general health consequences.

Sex-differences in physiological and oxidative stress responses and heavy metals burden in the black jaw tilapia, Sarotherodon melanotheron from a tropical freshwater dam (Nigeria).

Black jaw tilapia (S. melanotheron) collected from Awba Dam (a watershed recipient of effluents from anthropogenic sources) was analysed for biological responses and trace metal burden with the aim of evaluating sex differences in these responses as a measure of environmental and biota health status. Concentration of heavy metals in fish muscle were analysed and showed values above WHO permissible limits for food. Physiological and biochemical responses (AST, ALT, ALP, total protein, albumin, globulin, BUN, Na+, K+ and creatinine) showed apparent sex-related differences. Among antioxidant defence system, mt mRNA and proteins showed higher levels in male S. melanotheron. CAT and GPx activities were significantly higher in males than in female fish, while the corresponding mRNA showed an opposite trend with higher transcripts in females than males. Further, while GST activity was apparently higher in females, GR activity was significantly higher in males, compared to female fish. ZnCu-sod mRNA showed significantly higher transcript levels in males, than females. Overall, the trend in these biological responses showed direct relationship with heavy metal concentrations in fish muscle and sediment. These relationships were revealed using PCA that produced significant groupings between physiological, biochemical and oxidative stress variables and muscle/sediment trace element burden. Specifically, the PCA biplots showed that these biological variables were due to the presence of the heavy metals in the dam. Therefore, the sex differences in the measured cellular adaptations of S. melanotheron in relation to the toxic conditions at the dam may have significant consequences on osmotic balance and cellular processes that regulate biochemical/antioxidant responses towards metabolism, behaviour, growth, reproduction and survival of Awba dam resident fish species. Thus, these responses should be compared to a suitable reference site to discern the extent of effects of the Awba Dam biota for monitoring purposes.

Biochemical and endocrine-disrupting effects in Clarias gariepinus exposed to the synthetic pyrethroids, cypermethrin and deltamethrin.

In the present study, we investigated plasma biochemical and steroid hormone responses, together with gonado-histopathological alterations in Clarias gariepinus exposed to sublethal concentrations of two synthetic pyrethroids (cypermethrin and deltamethrin). Fish were exposed to environmentally-relevant concentrations of cypermethrin at 0 (ethanol solvent control), 0.07, 0.014, 0.028, 0.056) and deltamethrin at 0.22, 0.44, 0.88 and 1.76 µg/L, for 7, 14, 21 and 28 days. Plasma enzyme (aspartate transaminase: AST, alanine transaminase: ALT and alkaline phosphatase: ALP) and steroid hormones (estradiol-17ß: E2, testosterone: T) levels were analyzed. Gonado-histopathological evaluation shows the presence of ovo-testis (intersex), oocytes atresia, cytoplasmic degeneration and clumping of vitellogenic oocytes in females, while male fish displayed enlargement and degeneration of testicular seminiferous tubules after 28 days exposure to cypermethrin and deltamethrin. Plasma biochemical analysis in pesticides exposed fish revealed that AST, ALT and ALP were significantly increased in a concentration-dependent manner. In addition, we observed respective and apparent concentration- and time-dependent increase and decrease of plasma E2 and T levels, compared to control. Interestingly, the significant increase in E2 levels paralleled gonadal ovo-testis (intersex) condition in exposed fish, indicating endocrine disruptive effects of cypermethrin and deltamethrin that favor the estrogenic pathway, in addition to overt negative consequences on reproductive, biochemical and physiological health of the exposed fish.

Assessing the effects of Awba dam sediment (Nigeria) on the steroidogenesis of H295R cells using different extraction methods.

In the present study, H295R human cells were used to investigate the endocrine disruptor potential of three different sediments extracts taken from a Nigerian tropical freshwater dam (Awba Dam), using three extraction methods that allowed a selective consideration of contaminants based on their binding affinity, which is mainly driven by polarity, to sediment particles. After exposure to different concentration of each extract, H295R cells were evaluated for the expression profiles of 10 steroidogenic enzyme genes and estradiol (E2) and testosterone (T) levels. Our results showed a comparable concentrated-related increase in the expression of 17ß-hsd1, 3ß-hsd2 and cyp21 in cells treated with the polar and non-polar extracts. The star, hmgr, cyp11b2 and 17ß-hsd4 were slightly decreased, in an apparent concentration-specific manner, after treatment with the polar extract and decreased in the non-polar treatment. The cyp11a and cyp17 showed an opposite trend in the polar and non-polar treatments. E2 was significantly higher in cell treated with the non-polar extract. Elutriate exposure produced less pronounced variation in mRNA and hormones levels. Overall the extract with non-polar compounds produced the most severe effects in H295R cells. Thus, direct ingestion of detritus and mud from fishes and other benthonic organisms represent possible transfer of contaminants in the trophic web, and mainly account for alteration of the endocrine system previously observed in fish from the same study site.

Xenobiotic biotransformation, oxidative stress and obesogenic molecular biomarker responses in Tilapia guineensis from Eleyele Lake, Nigeria.

Despite the important ecosystem and hydrological roles of coastal and inland waters, there are no established biomonitoring protocols for evaluating environmental, wildlife and human health for these coastlines in Nigeria. In the present study, contaminants tissue burden and effects at molecular and physiological levels, were investigated in Tilapia guineensis from a man-made lake (Eleyele Lake) that is used for municipal domestic water supply and compared to a reference site (Igboho Lake). Gene expression of phase I and II biotransformation systems, oxidative stress and obesogen responses were analyzed using real-time PCR, and these results were related to general health index (condition factor: CF) and muscle burden of trace metals, aliphatic and polycyclic aromatic hydrocarbons (PAHs). We observed a significant increase in phase I and II biotransformation systems, oxidative stress and obesogen responses in male and female fish from Eleyele lake compared with the reference site. Overall, our data showed significant relationships between biological responses and tissue concentrations of metals and PAHs for the Eleyele lake compared with the reference site. Given that a positive influence on genes and pathways associated with metabolic status has been previously associated with peroxisome proliferator activated receptors (PPARs), xenobiotic compounds that activate PPARs may produce changes in energy and metabolic processes, leading to obesity. The high CF (>1 = good health condition) observed, coupled with the high muscle burden of inorganic and organic contaminants in fish from Eleyele lake, suggest a potential obesogenic effect in these fishes. These findings represent co-relational evidence that the Eleyele lake is contaminated and consequently affecting biological and physiological integrity of organisms inhabiting the lake. These findings also suggest potential health risks for humans, since the lake is extensively used for domestic water supply and fisheries.

The intersex phenomenon in Sarotherodon melanotheron from Lagos lagoon (Nigeria): Occurrence and severity in relation to contaminants burden in sediment.

The correlation between endocrine active contaminants in the environment and alterations in reproductive development of Sarotherodon melanotheron from Lagos lagoon has been investigated. Sediment and a total of 155 fish (74 males and 81 females) were collected between November 2014-March 2015 from selected contaminated sites (Ikorodu, Oworonshoki, Makoko and Idumota) and a putative control site (Igbore) along the lagoon. Sediment contaminant analysis revealed, significantly higher concentration of lindane, dieldrin, 4-iso-nonylphenol, 4-t-octylphenol and monobutyltin cation at the contaminated sites. Examination of gross morphological and histological changes of fish gonads showed a 27.4% prevalence of intersex in the sampled fish, of which 78% were males (testes-ova) and 22% were females (ovo-testis). Quantitative PCR (qPCR) of liver transcripts revealed the presence of vitellogenin (vtg) levels in male fish from contaminated sites. Zona radiata proteins (zrp) mRNA levels were significantly higher in females, compared to male fish. In general, significantly lower vtg and zrp transcripts levels were recorded at Igbore (control site), compared with contaminated sites. Principal component analysis (PCA) showed site and sex relationship in biological responses and contaminants, including trace metals, demonstrating that measured endocrine responses in fish were associated with contaminant burden in sediment. In addition, positive relationships were observed in male fish from Idumota, Oworonshoki and Ikorodu with vtg and dieldrin/4-iso-nonyphenol, with higher levels in male fish, compared to females. Further, contaminants from the Makoko, Oworonshoki and Ikorodu sites were positively associated with higher GSI and zrp in females. More importantly, the severity of intersex and changes in vtg transcripts imply a progressive feminization of male fish with concomitant alteration in the reproductive health of fish inhabiting the Lagos lagoon.

Endocrine disruptor responses in African sharptooth catfish (Clarias gariepinus) exposed to di-(2-ethylhexyl)-phthalate.

In the present study, we have investigated the endocrine disruptive effects of waterborne di-(2-ethylhexyl) phthalate (DEHP: 0 (control), 10, 100, 200 and 400 µg/L) on juvenile Clarias gariepinus by analyzing transcript patterns for hepatic vitellogenin (vtg), estrogen receptor-α (er-α), aromatase (cyp19a1b) and peroxisome proliferator activated receptor-α (ppar-α) using quantitative real-time PCR after 3, 7 and 14 days exposure period. In addition, we analyzed CYP19 and PPAR protein levels using enzyme-linked immunosorbent assay (ELISA), while cellular testosterone (T) and 17ß-estradiol (E2) levels were measured using enzyme immune assay (EIA). Tissue burden of DEHP was measured in the liver using gas chromatography-mass spectroscopy (GC-MS). We observed apparent concentration- and time-dependent increases of vtg, er-α, cyp19a1b transcripts, E2 and T levels after exposure to DEHP. A biphasic pattern of effect was observed for ppar-α, showing a concentration-dependent increase that peaked at 100 µg/L and thereafter, an apparent concentration-dependent decrease at 200 and 400 µg/L at all exposure days. Given that the post-differentiation changes of gonads in C. gariepinus corresponded with the 14-day sampling period, we separated the analyzed data into sexes after histological examination of the gonads, showing that females responded stronger, compared to males, to DEHP exposure at all exposure concentrations. Oocyte atresia, intersex (ovotestis) and karyoplasmic clumping were observed in females while male fish showed distortion and degeneration of seminiferous tubules and condensation of tubular cells in the 400 µg/L exposure group after 14 days. Corresponding canonical analysis (CCA) of all analyzed variables revealed a positive relationship between analyzed biological variables with increasing DEHP concentrations. Overall, molecular, biochemical and physiological responses presented in the present study indicate that exposure of C. gariepinus to waterborne DEHP produced endocrine disruptive responses with potential consequences for overt reproduction, development, physiology and general health of fish populations inhabiting phthalate contaminated aquatic environments. These responses represent valuable and effective biomarkers of exposure and effects, that can be adopted for screening the presence of EDCs in Nigeria and other developing countries.

Biphasic modulation of neuro- and interrenal steroidogenesis in juvenile African sharptooth catfish (Clarias gariepinus) exposed to waterborne di-(2-ethylhexyl) phthalate.

Receptor (i.e. genomic) and non-receptor (or non-genomic) effects of endocrine toxicology have received limited or almost non-existent attention for tropical species and regions. In the present study, we have evaluated the effects of di-(2-ethylhexyl) phthalate (DEHP) on neuro- and interrenal steroidogenesis of the African catfish (Clarias gariepinus) using molecular, immunochemical and physiological approaches. Juvenile fish (mean weight and length: 5.6±0.6g and 8.2±1.2cm, respectively), were randomly distributed into ten 120L rectangular glass tanks containing 60L of dechlorinated tap water, at 50 fish per exposure group. The fish were exposed to environmentally relevant concentrations of DEHP, consisting of 0 (ethanol solvent control), 10, 100, 200, and 400µg DEHP/L water and performed in two replicates. Brain, liver and head kidney samples were collected at day 3, 7 and 14 after exposure, and analysed for star, p450scc, cyp19a1, cyp17, cyp11ß-, 3ß-, 17ß- and 20ß-hsd, and 17ß-ohase mRNA expression using real-time PCR. The StAR, P450scc and CYP19 proteins were measured using immunoblotting method, while estradiol-17ß (E2) and testosterone (T) were measured in liver homogenate using enzyme immunoassay (EIA). Our data showed a consistent and unique pattern of biphasic effect on star and steroidogenic enzyme genes with increases at low concentration (10µg/L) and thereafter, a concentration-dependent decrease in both the brain and head kidney, that paralleled the expression of StAR, P450scc and CYP19 proteins. Cellular E2 and T levels showed an apparent DEHP concentration-dependent increase at day 14 of exposure. The observed consistency in the current findings and in view of previous reports on contaminants-induced alterations in neuro- and interrenal steroidogenesis, the broader toxicological and endocrine disruptor implication of our data indicate potentials for overt reproductive, metabolic, physiological and general health consequences for the exposed organisms.

Gross pathology, physiological and toxicological responses in relation to metals and persistent organic pollutants (POPs) burden in tilapia species from Ogun River, Nigeria.

We have investigated gross pathology, physiological (steroid precursors) and toxicological responses (oxidative stress and phase II biotransformation) in relation to tissue contaminant burden in Tilapia species along the entire length (320 km) of Ogun River, Nigeria. The Ogun River is the longest and largest river in Southwestern Nigeria located along heavily industrialized cities and receives complex mixtures of effluents. A total of 1074 tilapias were collected from three sampling points (Abeokuta, Isheri and Ikorodu) and from an upstream control point (Igboho) and evaluated for gross pathological changes, hepatic transcript levels for oxidative stress and phase II biotransformation responses. Trace metal concentrations and POPs in muscle samples were analyzed using ICP-MS and GC-MS respectively. Evaluation of gross pathological changes showed a 50-, 33-, 17 and 0% prevalence of hepatic tumors at the Ikorodu, Abeokuta, Isheri and Igboho sites, respectively. Plasma concentrations of cholesterol and pregnenolone showed apparent significant decreases at downstream sites of the control point in both male and female fish (except for pregnenolone levels of male fish at Ikorodu). Inversely, gst, ugt-1, ZuCu-sod and sod significantly increased in fish collected from downstream sites, compared with the control site and these increases paralleled the significant increase in trace metal and POPs concentrations at these sites. PCA revealed a site related association between measured toxicological responses and contaminant burden, indicating a potential cause-and-effect relationship. Thus, the possible adaptation of Ogun River Tilapia species to contaminants may have significant consequences on cellular, physiological and biochemical processes regulating metabolism, growth, development and reproduction, and also have serious human health consequences, since the Ogun River is used for fisheries and domestic water supply for surrounding neighborhoods.

Gonado-histopathological changes, intersex and endocrine disruptor responses in relation to contaminant burden in Tilapia species from Ogun River, Nigeria.

In the present study, we investigated the occurrence of intersex condition, histopathological changes in the gonad and endocrine disruptor biomarker responses in Tilapia species (Tilaipia guineensis, Sarotherodon galileaus and Oreochromis niloticus) along the Ogun River, Nigeria. The study sites covered a length of 320 km and a total of 1074 tilapias were collected from three sampling sites (Abeokuta, Isheri and Ikorodu) with different degrees of anthropogenic contamination. Samples were also collected from an upstream putative control site (Igboho) along the Ogun River. Hepatic transcript levels for vitellogenin (Vtg), zona radiata (Zrp) and aromatase (cyp19a1) were analyzed using real-time PCR. Gross gonadal morphology revealed a 24% prevalence of intersex showing visible testis and ovary in phenotypic females (25.4%) or males (74.6%). The intersex condition paralleled histopathological changes (ovotestis or testis-ova) in the gonads of female and male fish, respectively. Plasma concentrations of luteinizing hormone (LH), follicle stimulating hormone (FSH), 11-ketotestosterone (11-KT) and estradiol-17ß (E2) were measured using enzyme immunoassay, showing that male fish from downstream of the control site had significantly higher plasma E2, LH, and FSH concentrations compared to females. Similarly, Vtg, Zrp and cyp19a1 mRNA was significantly higher in males, compared to females. Analysis of contaminants showed the presence of 15 PCB congeners, lindane and dieldrin, and 4-iso-nonylphenol (4-iso-NP) and 4-tert-octylphenol (4-tert-OP) in fish muscle and sediment samples from Ogun River. Principal component analysis (PCA) revealed site and sex relationships between measured biological responses to groups of environmental contaminants, showing that the endocrine disruptive responses in fish were associated with biota and sediment contaminant burden. In addition, strong positive correlations were observed between male fish and Zrp, cyp19a1, E2, LH, FSH, PCBs, 4-iso-NP and 4-tert-OP, suggesting possible feminization effects of these contaminants on the male. In female fish, PCBs, 4-iso-NP and 4-tert-OP showed positive relationships with 11-KT and gonadosomatic index (GSI), suggesting masculinization effects by these contaminants. Overall, our findings demonstrate a causal relationship between endocrine disruption and contaminants burden in Tilapias species from Ogun River.

Concentration of polychlorinated biphenyl (PCB) congeners in the muscle of Clarias gariepinus and sediment from inland rivers of southwestern Nigeria and estimated potential human health consequences.

The distributions of polychlorinated biphenyl (PCB) congeners were determined in sediment and muscle of the African sharptooth catfish (Clarias gariepinus) from the Ogun and Ona rivers, southwest Nigeria. In addition, the effect of PCB congeners on condition factor (CF) and associated human health risk was assessed using muscle levels for a noncarcinogenic hazard quotient (HQ) calculation. Elevated concentrations of high-molecular-weight (HMW) PCB congeners were detected in sediment and fish downstream of discharge points of both rivers. A significant reduction in fish body weight and CF was observed to correlate with high PCB congener concentrations in the Ona River. A principal component (PC) biplot revealed significant site-related PCB congener distribution patterns for HMW PCB in samples from the Ogun River (71.3%), while the Ona River (42.6%) showed significant PCB congener patterns for low-molecular-weight (LMW) congeners. Biota-sediment accumulation factor (BSAF) was higher downstream for both rivers, presenting PCB congener-specific accumulation patterns in the Ona River. Significant decreases in fish body weight, length and CF were observed downstream compared to upstream in the Ona River. The non-carcinogenic HQ of dioxin-like congener 189 downstream in both rivers exceeded the HQ = 1 threshold for children and adults for both the Ogun and Ona rivers. Overall, our results suggest that industrial discharges contribute significantly to PCB inputs into these rivers, with potential for significant health implications for neighboring communities that utilize these rivers for fishing and other domestic purposes.