Antihyperglycemic and antihyperlipidemic effects of fruit extract of Hodgsonia heteroclita (Roxb.) Hook. f. & Thomson in diabetic mice.

ETHNOPHARMACOLOGICAL RELEVANCE: Hodgsonia heteroclita has been known as an important traditionally consumed medicinal plant of North-East India known to have antidiabetic properties. This study aims to investigate the effects of the ethanolic fruit extract of Hodgsonia heteroclita against hyperglycemia and hyperlipidemia by using streptozotocin (STZ) treated diabetic mice. MATERIALS AND METHODS: The fruits of H. heteroclita were collected from the various parts of Kokrajhar district, Assam India (Geographic coordinates: 26°24'3.85″ N 90°16'22.30″ E). Basic morphological evaluations were carried out by the Botanical Survey of India, Eastern circle, Shillong, who also certified and identified the plant. Hexane, chloroform, and ethanolic extracts of the fruit of H. heteroclita were investigated for α-amylase inhibition assay as a rapid screening tool for examining anti-diabetic activity. The efficacy of ethanolic extract at a dose of 100, 200, and 300 mg/kg body weight was tested for 21 days in STZ-induced diabetic mice. The body weight, fasting plasma glucose and serum lipids, and hepatic glycogen levels were measured in experimental animals to examine the antihyperglycemic and antihyperlipidemic efficacy of the extract. Both HPTLC and LC-MS analysis was performed to examine the phyotochemicals present in the ethanolic extract of H. heteroclita. RESULTS: It has been observed that treatment with the ethanolic extract dose-dependently reduced the plasma glucose levels, total cholesterol, low density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol, triglyceride, and increased the body weight, liver glycogens and high-density lipoprotein-cholesterol in STZ treated diabetic mice. HPTLC demonstrated the presence of triterpene compounds and LC-MS analysis revealed the presence Cucurbitacin I, Cucurbitacin E, and Kuguacin G as the triterpene phytoconstituents. CONCLUSION: The present study demonstrated that ethanolic fruit extract of H. heteroclita improved both glycemic and lipid parameters in mice model of diabetes.

Green biosynthesis, characterisation and antimicrobial activities of silver nanoparticles using fruit extract of Solanum viarum.

Green synthesis of nanoparticles is considered an efficient method when compared with chemical and physical methods because of its bulk production, eco-friendliness and low cost norms. The present study reports, for the first time, green synthesis of silver nanoparticles (AgNPs) at room temperature using Solanum viarum fruit extract. The visual appearance of brownish colour with an absorption band at 450 nm, as detected by ultraviolet-visible spectrophotometer analysis, confirmed the formation of AgNPs. X-ray diffraction confirmed the AgNPs to be crystalline with a face-centred lattice. The transmission electron microscopy-energy dispersive X-ray spectroscopy image showed the AgNPs are poly-dispersed and are mostly spherical and oval in shape with particle size ranging from 2 to 40 nm. Furthermore, Fourier transform-infrared spectra of the synthesised AgNPs confirmed the presence of phytoconstituents as a capping agent. The antimicrobial activity study showed that the AgNPs exhibited high microbial activity against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus susp. aureus, Aspergillus niger, and Candida albicans. The highest antimicrobial activity of AgNPs synthesised by S. viarum fruit extract was observed in P. aeruginosa, S. aureus susp. aureus and C. albicans with zone of inhibition, 26.67 mm.

Ethnomedicobotanical study of indigenous knowledge on medicinal plants used for the treatment of reproductive problems in Nalbari district, Assam, India.

ETHNOPHARMACOLOGICAL RELEVANCE: Reproductive problems are becoming the most predominant health care problems in many countries. World Health Organization (WHO), in 2012, also exemplified maternal illnesses, for instance, birth asphyxia and post birth difficulties are rising at an alarming rate. In addition, not all abnormalities of the human reproductive system have the same origin; the effects of reproductive problems would likely been affected by both male and female. For easy accessibility and affordability, medicinal plants are playing crucial role in primary healthcare services in India and their use is moreover, an integral part of the cultural heritage. However, our growing understanding of the human reproductive problems are segregated and scanty for herbal medications. AIM OF THE STUDY: To document the local name of the medicinal plants used by both male and female from indigenous knowledge for the treatment of reproductive ailments and to explore their biological and pharmacological confirmation and to address the class of secondary metabolites present therein. MATERIALS AND METHODS: The results stem in September 2015-April 2016 from an array of informations that were collected by direct interviews of the traditional medicinal practitioners in three villages, viz. Jaha, Niz-Bahjani and Madhupur of the southern Nalbari district, Assam. Both semi-structured and open-ended interview schedule was carried out with purposively selected individuals and focus group discussion (FGD) in the three selected sites for this study. Systematic analysis of fidelity level percentage (FL %), preference ranking percentage (PR %) and formulation scoring (FS) were calculated. Electronic databases such as Google, Google Scholar, and ScienceDirect were also been used to search existing pharmacological citations. Finally, qualitative chemical profiling were executed for the plants whose PR % scores ≥ 65. RESULTS: A total of 71 plant species belonging to 48 families and 64 genera are reported to be used for the treatment of several reproductive problems such as, infertility in male, impotence, erectile dysfunctioning, retrograde ejaculation and sexual potency in men and in women, aphrodisiac, metrorrhagia, infertility, dysmenorrhea and/or post birth difficulties under mono (57), di (17) and poly (8) herbal formulations. Herbs (40.85%) were reported as the most common lifeform, whereas leaves (22.54%) and the roots (22.54%) were the commonly used plant parts against different ailments. Extracts of Abroma augusta L. (stem) (FL: 66.67%, PR: IM-77.33%, L-65.33, FS: 2), Morinda angustifolia Roxb. (bark) (FL: 64.29%, PR: L-68%, FS: 1), Hodgsonia heteroclita Roxb. (fruit) (FL: 63.64%, PR: IF-65.33, FS: 0.25) and Hibiscus mutabilis L. (stem) (FL: 40%, PR: IM-68%, IIM: 65.33%, IML-68%, FS: 2) were than selected for further phytochemical analysis. CONCLUSIONS: Of the 71 plants used for reproductive management system, the highest number of plants were used for the treatment of irregular menstruation (22) followed by infertility (in both men and women) (19), vaginal disorder and leucorrhoea (9) and sexual potency (8). The qualitative chemical profiling have demonstrated the presence of alkaloids, carbohydrates, phenolic compounds, flavonoids and phytosterols, in maximum concentrations. Additionally, these data may be the most important resource for the new discovery of many bioactive principles.