RESUMEN
An epidemiological survey of honey bee viruses was conducted on 87 clinically healthy beehives located in southeastern Morocco. The sampled colonies were analyzed by reverse transcriptase (RT)-PCR / Real Time RT-qPCR with the aim of detecting and / or quantifying the following viruses: acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), sacbrood virus (SBV), black queen cell virus (BQCV), Kashmir bee virus (KBV) and Israeli acute paralysis virus (IAPV). With the exception of the last two of these viruses, all the other five were detected with different prevalence rates. DWV showed the highest prevalence rate (89.65 %), followed by BQCV (17.24 %), ABPV (8.04 %), CBPV (4.59 %), and SBV (2.29 %). This study represents the first molecular detection of BQCV in the country. Among all investigated colonies, only eight were virus free (9.2 %). By contrast, single infection was detected in 64.37 % of colonies, 21.8 % showed mixed infection with two viruses, while 4.6 % showed three. Nucleotide sequences of a portion of the DWV polyprotein gene obtained for six honey bee samples showed the greatest nucleotide identity with sequences of DWV from Sweden and Ireland. The negative effect of migratory beekeeping as opposed to stationary beekeeping was highlighted given that stationary beehives showed infection with up to three viruses only, while migratory beehives showed up to five viruses. The results of this study are of crucial importance as they shed light on the current status of honey bee health in southeastern Morocco.
Asunto(s)
Virus de Insectos , Abejas/virología , Animales , Marruecos/epidemiología , Virus de Insectos/genética , Virus de Insectos/aislamiento & purificación , Virus de Insectos/clasificación , Virus ARN/genética , Virus ARN/aislamiento & purificación , Virus ARN/clasificación , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Prevalencia , Dicistroviridae/genética , Dicistroviridae/aislamiento & purificación , Virosis/epidemiología , Virosis/virología , Virosis/veterinaria , FilogeniaRESUMEN
PURPOSE: Understanding the factors influencing parasite diversification, their distribution and infection patterns among hosts provides valuable information on the impact of parasitism on natural populations and the dynamics and evolution of disease outbreaks. In this study, we investigated prevalence and intensity of infection by blood parasites, the diversity, distribution, host specificity and infection patterns of apicomplexan parasites in lizards of Morocco. METHODS: Samples from lizard specimens were collected between 2019 and 2021 from different prospected localities in Morocco. In total, 1500 lizards were collected, and their blood and tail tips were screened for blood parasites. RESULTS: Using a PCR approach, we detected two distinct apicomplexan parasite groups (haemogregarines and haemococcidians) and also Stramenopiles (Proteromonas). A significant difference was identified between hosts families, with no agamids or skinks infected and with particularly high infection rates in lacertids. Twenty-four samples were sequenced and included in a phylogenetic assessment. Two samples of Atlantolacerta andreanskyi were infected with unidentified species of Proteromonas. Three samples were infected with haemococcidians, including two samples with Schellackia and another sample with Lankesterella, more closely related to others isolated from bird hosts. These are the first reports of Lankesterella and Proteromonas in these lizards from Morocco. Nineteen different isolates of the complex of Hepatozoon/Karyolysus were identical or very similar to previously sequenced parasites from North Africa and Iberian Peninsula and included new host species. CONCLUSION: Our results highlight the need for future research on the vectors involved in transmission that may influence the ecology and distribution patterns of these parasites. Given that Morocco is a hot spot of endemicity and reptile richness, our results are relevant not only in the context of their taxonomy, but can also have implications for conservation.
Asunto(s)
Eucoccidiida , Lagartos , Humanos , Animales , Lagartos/parasitología , Marruecos/epidemiología , Filogenia , Análisis de Secuencia de ADNRESUMEN
Morocco has a great diversity of reptiles; more than 26% of the herpetofauna species are considered to be endemic. Nevertheless, there is little information available on helminth parasites of Moroccan lizards. The purpose of this article is to establish a helminth list using non-invasive methods for natural populations of seven lizard species: Acanthodactylus maculatus, Chalcides mionecton, Chalcides montanus, Chalcides polylepis, Quedenfeldtia moerens, Quedenfeldtia trachyblepharus, and Tarentola mauritanica. For each species, prevalence and intensity of infection were given as well as their relationship to some determinant parameters (sex, age class, size, altitude, and seasons). Four species of nematodes, Parapharyngodon micipsae, Pharyngodon mamillatus, Spauligodon auziensis, Thelandros alatus, and unassigned Spauligodon were found. Mean helminth intensity for the seven lizard species was 7.3 ± 16.2 SD (range = 1-92 in infected lizards), with total infection prevalence of 5.6%. Mixed infections were observed in Chalcides montanus and Chalcides polylepis co-infected by Pharyngodon mamillatus and Spauligodon sp. Furthermore, the intensity of infection by nematodes were significantly different among the seven studied species. The nematode infection in Chalcides mionecton were related to sex, host size, and altitude; in Chalcides polylepis to the host age; in Quedenfeldtia trachyblepharus to elevation, age, and host size; and in Tarentola Mauritanica to age, body size, seasons, and altitude. In conclusion, our study provides data about nine new host and locality records and the effect of some determinant factors on host parasitism.
Asunto(s)
Helmintiasis , Helmintos , Lagartos , Nematodos , Infecciones por Nematodos , Oxyuroidea , Animales , Lagartos/parasitología , Marruecos/epidemiología , Infecciones por Nematodos/epidemiologíaRESUMEN
In Morocco, there are two well-recognised honey bee (Apis mellifera L.) subspecies: A. m. intermissa in the north and A. m. sahariensis in the south-east. The latter subspecies is found in the arid and semiarid climates of the Sahara Desert. In this study, we used honey bees from four areas of south-eastern Morocco which are, to some degree, isolated by arid zones. We analysed the shape and size of the forewings, using the method of geometric morphometrics. The bees from the four areas of south-eastern Morocco differed significantly in terms of wing shape. Moreover, bees from traditional hives were smaller than those from modern hives. The bees from south-eastern Morocco were clearly different from the reference samples obtained from the Morphometric Bee Data Bank in Oberursel, Germany, representing most of the global variation in honey bees. Surprisingly, the bees were also different from A. m. sahariensis, which should occur in the study area, according to earlier studies. This difference could have been caused by introgression with non-native subspecies imported by beekeepers. The distinct honey bees from south-eastern Morocco deserve to be protected. We provide a method for identifying them, which can help protect them.
RESUMEN
The present work was undertaken to investigate the occurrence of ectoparasites (mites and ticks) and endoparasites (haemogregarines and helminths) in the Atlas day gecko, Quedenfeldtia trachyblepharus (Boettger, 1874), a high-altitude Moroccan endemic lizard. The study examinated also the effect of some host parameters (age, sex, size, body condition), in addition to the season and altitude on the prevalence and intensity of parasite infestations. The study was conducted in three localities from May to September 2019. The results indicated that 35% of juvenile geckos were found to be parasited by one type of parasite. Contrarily, up to three types of parasites were detected in the adults. The prevalence of mite infestations was 48.02%, with a mean intensity of 11.80 ± 15.69. The patterns of mite infestations was found to be mainly related to the altitude, while the prevalence and intensity of infestations were linked to the host size, and to the sex and season, respectively. Larvae and nymphs of Ixodes ricinus (Linnaeus, 1758) were the only life stages infesting geckos, with a prevalence and mean intensity of infestations of 4.41% and 2.2 ± 1.48, respectively. The tick infestations observed were mainly related to the season. The prevalence and intensity of haemogregarine infections were, respectively, 7.92% and 0.24 ± 0.15. The altitude was found to be the only factor associated with this infection. This study also revealed the presence of one helminth genus, Spauligodon sp., with a prevalence and mean intensity of 12.33% and 1.46 ± 0.88, respectively. Spauligodon infestations was significantly associated with age, host size and altitude. This finding represents the first citation of this parasite in Quedenfeldtia genus. Our study indicated that there was no significant relationship between parasite load and geckos body condition, which suggested a stable interaction between the gecko and its parasites. However, it showed a difference of infection between the localities, which could be in relation with habitat conditions.