Revealing speckle obscured living human retinal cells with artificial intelligence assisted adaptive optics optical coherence tomography.

BACKGROUND: In vivo imaging of the human retina using adaptive optics optical coherence tomography (AO-OCT) has transformed medical imaging by enabling visualization of 3D retinal structures at cellular-scale resolution, including the retinal pigment epithelial (RPE) cells, which are essential for maintaining visual function. However, because noise inherent to the imaging process (e.g., speckle) makes it difficult to visualize RPE cells from a single volume acquisition, a large number of 3D volumes are typically averaged to improve contrast, substantially increasing the acquisition duration and reducing the overall imaging throughput. METHODS: Here, we introduce parallel discriminator generative adversarial network (P-GAN), an artificial intelligence (AI) method designed to recover speckle-obscured cellular features from a single AO-OCT volume, circumventing the need for acquiring a large number of volumes for averaging. The combination of two parallel discriminators in P-GAN provides additional feedback to the generator to more faithfully recover both local and global cellular structures. Imaging data from 8 eyes of 7 participants were used in this study. RESULTS: We show that P-GAN not only improves RPE cell contrast by 3.5-fold, but also improves the end-to-end time required to visualize RPE cells by 99-fold, thereby enabling large-scale imaging of cells in the living human eye. RPE cell spacing measured across a large set of AI recovered images from 3 participants were in agreement with expected normative ranges. CONCLUSIONS: The results demonstrate the potential of AI assisted imaging in overcoming a key limitation of RPE imaging and making it more accessible in a routine clinical setting.

The retinal pigment epithelium (RPE) is a single layer of cells within the eye that is crucial for vision. These cells are unhealthy in many eye diseases, and this can result in vision problems, including blindness. Imaging RPE cells in living human eyes is time consuming and difficult with the current technology. Our method substantially speeds up the process of RPE imaging by incorporating artificial intelligence. This enables larger areas of the eye to be imaged more efficiently. Our method could potentially be used in the future during routine eye tests. This could lead to earlier detection and treatment of eye diseases, and the prevention of some causes of blindness.

Widespread subclinical cellular changes revealed across a neural-epithelial-vascular complex in choroideremia using adaptive optics.

Choroideremia is an X-linked, blinding retinal degeneration with progressive loss of photoreceptors, retinal pigment epithelial (RPE) cells, and choriocapillaris. To study the extent to which these layers are disrupted in affected males and female carriers, we performed multimodal adaptive optics imaging to better visualize the in vivo pathogenesis of choroideremia in the living human eye. We demonstrate the presence of subclinical, widespread enlarged RPE cells present in all subjects imaged. In the fovea, the last area to be affected in choroideremia, we found greater disruption to the RPE than to either the photoreceptor or choriocapillaris layers. The unexpected finding of patches of photoreceptors that were fluorescently-labeled, but structurally and functionally normal, suggests that the RPE blood barrier function may be altered in choroideremia. Finally, we introduce a strategy for detecting enlarged cells using conventional ophthalmic imaging instrumentation. These findings establish that there is subclinical polymegathism of RPE cells in choroideremia.

Photoreceptor and Retinal Pigment Epithelium Relationships in Eyes With Vitelliform Macular Dystrophy Revealed by Multimodal Adaptive Optics Imaging.

Purpose: To assess the structure of cone photoreceptors and retinal pigment epithelial (RPE) cells in vitelliform macular dystrophy (VMD) arising from various genetic etiologies. Methods: Multimodal adaptive optics (AO) imaging was performed in 11 patients with VMD using a custom-assembled instrument. Non-confocal split detection and AO-enhanced indocyanine green were used to visualize the cone photoreceptor and RPE mosaics, respectively. Cone and RPE densities were measured and compared across BEST1-, PRPH2-, IMPG1-, and IMPG2-related VMD. Results: Within macular lesions associated with VMD, both cone and RPE densities were reduced below normal, to 37% of normal cone density (eccentricity 0.2 mm) and to 8.4% of normal RPE density (eccentricity 0.5 mm). Outside of lesions, cone and RPE densities were slightly reduced (both to 92% of normal values), but with high degree of variability in the individual measurements. Comparison of juxtalesional cone and RPE measurements (<1 mm from the lesion edge) revealed significant differences in RPE density across the four genes (P < 0.05). Overall, cones were affected to a greater extent than RPE in patients with IMPG1 and IMPG2 pathogenic variants, but RPE was affected more than cones in BEST1 and PRPH2 VMD. This trend was observed even in contralateral eyes from a subset of five patients who presented with macular lesions in only one eye. Conclusions: Assessment of cones and RPE in retinal locations outside of the macular lesions reveals a pattern of cone and RPE disruption that appears to be gene dependent in VMD. These findings provide insight into the cellular pathogenesis of disease in VMD.

In-vivo sub-diffraction adaptive optics imaging of photoreceptors in the human eye with annular pupil illumination and sub-Airy detection.

Adaptive optics scanning light ophthalmoscopy (AOSLO) allows non-invasive visualization of the living human eye at the microscopic scale; but even with correction of the ocular wavefront aberrations over a large pupil, the smallest cells in the photoreceptor mosaic cannot always be resolved. Here, we synergistically combine annular pupil illumination with sub-Airy disk confocal detection to demonstrate a 33% improvement in transverse resolution (from 2.36 to 1.58 µm) and a 13% axial resolution enhancement (from 37 to 32 µm), an important step towards the study of the complete photoreceptor mosaic in heath and disease. Interestingly, annular pupil illumination also enhanced the visualization of the photoreceptor mosaic in non-confocal detection schemes such as split detection AOSLO, providing a strategy for enhanced multimodal imaging of the cone and rod photoreceptor mosaic.

Integrating adaptive optics-SLO and OCT for multimodal visualization of the human retinal pigment epithelial mosaic.

In vivo imaging of human retinal pigment epithelial (RPE) cells has been demonstrated through multiple adaptive optics (AO)-based modalities. However, whether consistent and complete information regarding the cellular structure of the RPE mosaic is obtained across these modalities remains uncertain due to limited comparisons performed in the same eye. Here, an imaging platform combining multimodal AO-scanning light ophthalmoscopy (AO-SLO) with AO-optical coherence tomography (AO-OCT) is developed to make a side-by-side comparison of the same RPE cells imaged across four modalities: AO-darkfield, AO-enhanced indocyanine green (AO-ICG), AO-infrared autofluorescence (AO-IRAF), and AO-OCT. Co-registered images were acquired in five subjects, including one patient with choroideremia. Multimodal imaging provided multiple perspectives of the RPE mosaic that were used to explore variations in RPE cell contrast in a subject-, location-, and even cell-dependent manner. Estimated cell-to-cell spacing and density were found to be consistent both across modalities and with normative data. Multimodal images from a patient with choroideremia illustrate the benefit of using multiple modalities to infer the cellular structure of the RPE mosaic in an affected eye, in which disruptions to the RPE mosaic may locally alter the signal strength, visibility of individual RPE cells, or even source of contrast in unpredictable ways.

Active Cell Appearance Model Induced Generative Adversarial Networks for Annotation-Efficient Cell Segmentation and Identification on Adaptive Optics Retinal Images.

Data annotation is a fundamental precursor for establishing large training sets to effectively apply deep learning methods to medical image analysis. For cell segmentation, obtaining high quality annotations is an expensive process that usually requires manual grading by experts. This work introduces an approach to efficiently generate annotated images, called "A-GANs", created by combining an active cell appearance model (ACAM) with conditional generative adversarial networks (C-GANs). ACAM is a statistical model that captures a realistic range of cell characteristics and is used to ensure that the image statistics of generated cells are guided by real data. C-GANs utilize cell contours generated by ACAM to produce cells that match input contours. By pairing ACAM-generated contours with A-GANs-based generated images, high quality annotated images can be efficiently generated. Experimental results on adaptive optics (AO) retinal images showed that A-GANs robustly synthesize realistic, artificial images whose cell distributions are exquisitely specified by ACAM. The cell segmentation performance using as few as 64 manually-annotated real AO images combined with 248 artificially-generated images from A-GANs was similar to the case of using 248 manually-annotated real images alone (Dice coefficients of 88% for both). Finally, application to rare diseases in which images exhibit never-seen characteristics demonstrated improvements in cell segmentation without the need for incorporating manual annotations from these new retinal images. Overall, A-GANs introduce a methodology for generating high quality annotated data that statistically captures the characteristics of any desired dataset and can be used to more efficiently train deep-learning-based medical image analysis applications.

Automated Iterative Label Transfer Improves Segmentation of Noisy Cells in Adaptive Optics Retinal Images.

High quality data labeling is essential for improving the accuracy of deep learning applications in medical imaging. However, noisy images are not only under-represented in training datasets, but also, labeling of noisy data is low quality. Unfortunately, noisy images with poor quality labels are exacerbated by traditional data augmentation strategies. Real world images contain noise and can lead to unexpected drops in algorithm performance. In this paper, we present a non-traditional, purposeful data augmentation method to specifically transfer high quality automated labels into noisy image regions for incorporation into the training dataset. The overall approach is based on the use of paired images of the same cells in which variable image noise results in cell segmentation failures. Iteratively updating the cell segmentation model with accurate labels of noisy image areas resulted in an improvement in Dice coefficient from 77% to 86%. This was achieved by adding only 3.4% more cells to the training dataset, showing that local label transfer through graph matching is an effective augmentation strategy to improve segmentation.

Spatially Aware Dense-LinkNet Based Regression Improves Fluorescent Cell Detection in Adaptive Optics Ophthalmic Images.

Retinal pigment epithelial (RPE) cells play an important role in nourishing retinal neurosensory photoreceptor cells, and numerous blinding diseases are associated with RPE defects. Their fluorescence signature can now be visualized in the living human eye using adaptive optics (AO) imaging combined with indocyanine green (ICG), which motivates us to develop an automated RPE detection method to improve the quantitative evaluation of RPE status in patients. This paper proposes a spatially-aware, Dense-LinkNet-based regression approach to improve the detection of in vivo fluorescent cell patterns, achieving precision, recall, and F1-Score of 93.6 ± 4.3%, 81.4 ± 9.5%, and 86.7 ± 5.7%, respectively. These results demonstrate the utility of incorporating spatial inputs into a deep learning-based regression framework for cell detection.

Deriving Visual Cues from Deep Learning to Achieve Subpixel Cell Segmentation in Adaptive Optics Retinal Images.

Direct visualization of photoreceptor cells, specialized neurons in the eye that sense light, can be achieved using adaptive optics (AO) retinal imaging. Evaluating photoreceptor cell morphology in retinal diseases is important for monitoring the onset and progression of blindness, but segmentation of these cells is a critical first step. Most segmentation approaches focus on cell region extraction, without directly considering cell boundary localization. This makes it difficult to track cells that have ambiguous boundaries, which result from low image contrast, anisotropic cell regions, or densely-packed cells whose boundaries appear to touch each other. These are all characteristics of the AO images that we consider here. To address these challenges, we develop an AOSeg-Net method that uses a multi-channel U-Net to predict the spatial probabilities of the cell boundary and obtain cell centroid and region distribution information as a means for facilitating cell segmentation. Five-color theorem guarantees the separation of any touching cells. Finally, a region-based level set algorithm that combines all of these visual cues is used to achieve subpixel cell segmentation. Five-fold cross-validation on 428 high resolution retinal images from 23 human subjects showed that AOSegNet substantially outperformed the only other existing approach with Dice coefficients [%] of 84.7 and 78.4, respectively, and average symmetric contour distances [µm] of 0.59 and 0.80, respectively.

Active Appearance Model Induced Generative Adversarial Network for Controlled Data Augmentation.

Data augmentation is an important strategy for enlarging training datasets in deep learning-based medical image analysis. This is because large, annotated medical datasets are not only difficult and costly to generate, but also quickly become obsolete due to rapid advances in imaging technology. Image-to-image conditional generative adversarial networks (C-GAN) provide a potential solution for data augmentation. However, annotations used as inputs to C-GAN are typically based only on shape information, which can result in undesirable intensity distributions in the resulting artificially-created images. In this paper, we introduce an active cell appearance model (ACAM) that can measure statistical distributions of shape and intensity and use this ACAM model to guide C-GAN to generate more realistic images, which we call A-GAN. A-GAN provides an effective means for conveying anisotropic intensity information to C-GAN. A-GAN incorporates a statistical model (ACAM) to determine how transformations are applied for data augmentation. Traditional approaches for data augmentation that are based on arbitrary transformations might lead to unrealistic shape variations in an augmented dataset that are not representative of real data. A-GAN is designed to ameliorate this. To validate the effectiveness of using A-GAN for data augmentation, we assessed its performance on cell analysis in adaptive optics retinal imaging, which is a rapidly-changing medical imaging modality. Compared to C-GAN, A-GAN achieved stability in fewer iterations. The cell detection and segmentation accuracy when assisted by A-GAN augmentation was higher than that achieved with C-GAN. These findings demonstrate the potential for A-GAN to substantially improve existing data augmentation methods in medical image analysis.

Comportamiento clínico de la neurotoxoplasmosis en pacientes con sida / Neurotoxoplasmosis Clinical Behavior in AIDS Patients

Se realizó, en el período de enero de 2007 a diciembre de 2009, un estudio descriptivo en el Servicio de Medicina Interna del Hospital General Universitario Vladimir Ilich Lenin de Holguín, con un universo de 84 pacientes con neurotoxoplasmosis y diagnóstico confirmado de sida para caracterizar su comportamiento clínico. Predominó el grupo de 25 - 34 años, masculinos y la principal enfermedad asociada fue la bronconeumonía. Un alto porcentaje no realizaba tratamiento antirretroviral y fallecieron 11 casos. En la mayoría de los pacientes el conteo de CD4 fue muy bajo, por ser casos sida en estadio terminal, con afecciones oportunistas severas, sin terapia antirretroviral, no obstante el número de fallecidos fue bajo; resultado que señala un manejo adecuado de los enfermos...(AU)

A descriptive study, at Internal Medicine Service at V I Lenin University General Hospital, Holguín was carried out. The universe comprised 84 patients with neurotoxoplasmosis and AIDS. The objective of this work was to characterize the disease clinical behavior from January 2007 to December 2009. The age group between 25 and 34 years, male sex prevailed. Bronchopneumonia was the main associated disease. Antiretroviral treatment was not given for a high percentage of the patient and 11 of them died. In most of the patients the count of CD4 was very low, because they were AIDS cases in terminal stage, with severe opportunistic affections, without antiretroviral therapy. The number of deceased patients was low. It demonstrated that there was an adequate management of the disease in this kind of patients...(AU)

Impacto de la vacuna antimeningocócica cubana en la morbilidad y mortalidad por enfermedad meningocócica / Cuban Meningococcal Vaccine Impact in Morbidity and Mortality

Se realizó un estudio longitudinal, retrospectivo de serie a 112 pacientes que ingresaron con diagnóstico de enfermedad meningocócica con aislamiento del germen en sangre, petequias o líquido cefalorraquídeo (LCR), en el Hospital Pediátrico Provincial Octavio de la Concepción de la Pedraja, de Holguín; durante un período de 18 años, desde enero de 1989 hasta diciembre de 2006 El objetivo de este trabajo fue conocer el impacto de la vacuna antimeningocócica cubana en la morbilidad y mortalidad por esta enfermedad Existió una notable disminución de los enfermos después de aplicada la vacuna Los menores de cuatro años fueron los más afectados. A partir de 1994 no se han producidos fallecimientos por esta causa en nuestro hospital...(AU)

A longitudinal, retrospective study of series in 112 patients that were admitted at Octavio de la Concepciòn y de la Pedraja Pediatric Hospital, Holguín during a period of 18 years, from January 1989 to December 2006 with meningococcal disease diagnosis with germ in blood, and petechia or cephalorrhachidian liquid was carried out, aimed at knowing the Cuban meningococcal vaccine impact There was a notable decrease of cases after the vaccine was applied. ChiIdren who were under 4 years were the most affected ones...(AU)

Relación de la ingesta de alimentos con la hipertensión arterial en pacientes de la Policlínica Pedro Díaz Coello / Intake Behavior in Hypertensive Patients: Pedro Díaz Coello Polyclinic. Holguín

Se realizó un estudio descriptivo de serie de casos sobre la ingesta en los pacientes con hipertensión arterial con el objetivo de determinar su comportamiento. A los enfermos seleccionados a través del muestreo probabilístico sistemático, con el método de números impares, se les realizó las valoraciones antropométricas, una encuesta de frecuencia semicuantitativa sobre consumo de alimentos y se utilizó su historia clínica. En la muestra predominaron los pacientes del sexo femenino, de más de 60 años y existió relación entre el peso corporal y las cifras de colesterol plasmático siendo ésta, directa y moderada (r 0,77). La ingesta se comportó poco saludable por ser hipercalórica con bajo consumo de vitaminas A y E, de calcio y fibra vegetal, con exceso de grasas saturadas sobre las insaturadas. La distribución porcentual calórico del total consumido evidencia una dieta muy pobre en proteínas y excesiva en carbohidratos...(AU)

A descriptive study of series of cases was carried out on the dietary intake in patients with hypertension aimed at determining its behavior. The patients were selected through a systematic probabilistic sampling using odd numbers, performing anthropometric assessments, a questionnaire of semiquantitative frequency on intake and their personal clinical histories review. The prevailing sex was the female one. There was a relationship between corporal weight and figures of plasmatic cholesterol. Intake was not very healthy, it was hypercaloric with a low consumption of vitamins A and E, calcium and vegetables, an excess of saturated fats on the unsaturated. The caloric percentual distribution demonstrated that the diet was poor in proteins and excessive in carbohydrate...(AU)