Authentication of duck blood tofu binary and ternary adulterated with cow and pig blood-based gel using Fourier transform near-infrared coupled with fast chemometrics.

This work aims to investigate a feasible and practical technique for the authentication of edible animal blood food (EABF) using Fourier transform near-infrared (FT-NIR) coupled with fast chemometrics. A total of 540 samples were used, including raw duck blood tofu (DBT), cow blood-based gel (CBG), pig blood-based gel (PBG), and DBT binary and ternary adulterated with CBG and PBG. The protein, fat, total sugar, and 16 kinds of amino acids were measured to validate the difference in basic organic matters among EABFs according to species. Fisher linear discriminate analysis (Fisher LDA) and extreme learning machine (ELM) were implemented comparatively to identify the adulterated EABF. To predict adulteration levels, four extreme learning machine regression (ELMR) models were constructed and optimized. Results showed that, by analyzing 27 crucial spectral variables, the ELM model provides higher accuracy of 93.89% than Fisher LDA for the independent samples. All the correlation coefficients of the optimized ELMR models' training and prediction sets were better than 0.94, the root mean square errors were all less than 3.5%, and the residual prediction deviation and the range error ratios were all higher than 4.0 and 12.0, respectively. In conclusion, the FT-NIR paired with ELM have great potential in authenticating the EABF. This work presents amino acids content in EABFs for the first time and built tracing models for rapid authentication of DBT, which can be used to manage the EABF market, thereby preventing illegal adulteration and unfair competition.

Machine-learning assisted modelling of multiple elements for authenticating edible animal blood food.

Elemental fingerprint coupled with machine learning modelling was proposed for species authentication of the edible animal blood gel (EABG). A total of 25 elements were determined by inductively coupled plasma mass spectrometry (ICP-MS) and atomic absorption spectroscopy (AAS) in 150 EABG samples prepared from five species of animals, namely duck, chicken, bovine, pig, and sheep. Extreme learning machine (ELM) models were constructed and optimized. Principal component analysis and Fisher linear discriminant analysis were comparatively utilized for dimension reduction of the crucial input elements selected via stepwise discriminant analysis and one-way ANOVA. The optimal ELM model was obtained with the crucial elements selected by one-way ANOVA from the relative content of the measured elements, which afforded accuracies of 98.0% and 96.0% for the training and test set, respectively. All findings suggest that elemental fingerprint accompanied by ELM have great potential in authenticating the edible animal blood food.

Fusion of a low-cost electronic nose and Fourier transform near-infrared spectroscopy for qualitative and quantitative detection of beef adulterated with duck.

A low-cost electronic nose (E-nose) based on colorimetric sensors fused with Fourier transform-near-infrared (FT-NIR) spectroscopy was proposed as a rapid and convenient technique for detecting beef adulterated with duck. The total volatile basic nitrogen, protein, fat, total sugar and ash contents were measured to investigate the differences of basic properties between raw beef and duck; GC-MS was employed to analyze the difference of the volatile organic compounds emitted from these two types of meat. For variable selection and spectra denoising, the simple T-test (p < 0.05) separately intergraded with first derivative, second derivative, centralization, standard normal variate transform, and multivariate scattering correction were performed and the results compared. Extreme learning machine models were built to identify the adulterated beef and predict the adulteration levels. Results showed that for recognizing the independent samples of raw beef, beef-duck mixtures, and raw duck, FT-NIR offered a 100% identification rate, which was superior to the E-nose (83.33%) created herein. In terms of predicting adulteration levels, the root means square error (RMSE) and the correlation coefficient (r) for independent meat samples using FT-NIR were 0.511% and 0.913, respectively. At the same time, for E-nose, these two indicators were 1.28% and 0.841, respectively. When the E-nose and FT-NIR data were fused, the RMSE decreased to 0.166%, and the r improved to 0.972. All the results indicated that fusion of the low-cost E-nose and FT-NIR could be employed for rapid and convenient testing of beef adulterated with duck.

Artificial bionic taste sensors coupled with chemometrics for rapid detection of beef adulteration.

The purpose of this study was to investigate the potential of taste sensors coupled with chemometrics for rapid determination of beef adulteration. A total of 228 minced meat samples were prepared and analyzed via raw ground beef mixed separately with chicken, duck, and pork in the range of 0 ~ 50% by weight at 10% intervals. Total sugars, protein, fat, and ash contents were also measured to validate the differences between raw meats. For sensing the water-soluble chemicals in the meats, an electronic tongue based on multifrequency large-amplitude pulses and six metal electrodes (platinum, gold, palladium, tungsten, titanium, and silver) was employed. Fisher linear discriminant analysis (Fisher LDA) and extreme learning machine (ELM) were used to model the identification of raw and the adulterated meats. While an adulterant was detected, the level of adulteration was predicted using partial least squares (PLS) and ELM and the results compared. The results showed that superior recognition models derived from ELM were obtained, as the recognition rates for the independent samples in different meat groups were all over 90%; ELM models were more precisely than PLS models for prediction of the adulteration levels of beef mixed with chicken, duck, and pork, with root mean squares error for the independent samples of 0.33, 0.18, and 0.38% and coefficients of variance of 0.914, 0.956, and 0.928, respectively. The results suggested that taste sensors combined with ELM could be useful in the rapid detection of beef adulterated with other meats.

An evaluation of biochemical, structural and volatile changes of dry-cured pork using a combined ion mobility spectrometry, hyperspectral and confocal imaging approach.

BACKGROUND: Food processing induces various modifications that affect the structure, physical and chemical properties of food products and hence the acceptance of the product by the consumer. In this work, the evolution of volatile components, 2-thiobarbituric acid reactive substances (TBARS), moisture content (MC) and microstructural changes of pork was investigated by hyperspectral (HSI) and confocal imaging (CLSM) techniques in synergy with gas chromatography-ion mobility spectrometry (GC-IMS). Models based on partial least squares regression (PLSR) were developed using the full HSI spectrum variables as well as optimum variables selected through a competitive adaptive reweighted sampling algorithm. RESULTS: Prediction results for MC and TBARS using multiplicative scatter correction pre-processed spectra models demonstrated greater efficiency and predictability with determination coefficient of prediction of 0.928, 0.930 and root mean square error of prediction of 0.114, 1.002, respectively. Major structural changes were also observed during CLSM imaging, which were greatly pronounced in pork samples oven cooked for 15 and 20 h. These structural changes could be related to the denaturation of the major meat components, which could explain the loss of moisture and the formation of TBARS visualized from the HSI chemical distribution maps. GC-IMS identified 35 volatile components, including hexanal and pentanal, which are also known to have a higher lipid oxidation specificity. CONCLUSION: The synergistic application of HSI, CLSM and GC-IMS enhanced data mining and interpretation and provided a convenient way for analyzing the chemical, structural and volatile changes occurring in meat during processing. © 2021 Society of Chemical Industry.

Monitoring microstructural changes and moisture distribution of dry-cured pork: a combined confocal laser scanning microscopy and hyperspectral imaging study.

BACKGROUND: Various spectral profiles, including reflectance, absorbance, and Kubelka-Munk spectra, have been derived from hyperspectral images and used to develop multivariate models to evaluate changes in the quality of meat and meat products as a function of processing. However, none of these has the capacity to produce images of the structural changes often associated with processing. This study explored the feasibility of combining hyperspectral imaging (HSI) with confocal laser scanning microscopy (CLSM) to examine the impact of processing on microstructural changes and the evolution of moisture. Reflectance spectra features were obtained and transformed into absorbance and Kubelka-Munk spectra and their ability to predict moisture content using models established on partial least-squares regression were evaluated. RESULTS: The partial least-squares regression model (full-band wavelength) dubbed Rs-MSC yielded the best result, with R c 2 = 0.967 , RMSEC = 0.127, R cv 2 = 0.949 , RMSECV = 0.418, R p 2 = 0.937 , RMSEP = 0.824. Next, a total of 16 optimum wavelengths were selected using the competitive adaptive reweighted sampling algorithm. These wavelengths also yielded good results for Rs-MSC, with R c 2 = 0.958 , RMSEC = 0.840, R cv 2 = 0.931 , RMSECV = 0.118, R p 2 = 0.926 , RMSEP = 0.121. Regarding moisture distribution and microstructure analysis, HSI and CLSM were able to reveal moisture content distribution and conformational differences in microstructure in the test samples. CONCLUSION: Using HSI in synergy with CLSM may offer a reliable means for assessing both the chemical and structural changes that occur in other congener food products during processing. © 2020 Society of Chemical Industry.

Application of volatile and spectral profiling together with multimode data fusion strategy for the discrimination of preserved eggs.

The maturity level of eggs during pickling is conventionally assessed by choosing few eggs from each curing batch to crack open. Yet, this method is destructive, creates waste and has consequences for financial losses. In this work, the feasibility of integrating electronic nose (EN) with reflectance hyperspectral (RH) and transmittance hyperspectral (TH) data for accurate classification of preserved eggs (PEs) at different maturation periods was investigated. Classifier models based solely on RH and TH with EN achieved a training accuracy (93.33%, 97.78%) and prediction accuracy (88.89%; 93.33%) respectively. The fusion of the three datasets, (EN + RH + TH) as a single classifier model yielded an overall training accuracy of 98.89% and prediction accuracy of 95.56%. Also, 52 volatile compounds were obtained from the PE headspace, of which 32 belonged to seven functional groups. This study demonstrates the ability to integrate EN with RH and TH data to effectively identify PEs during processing.

Integration of a low-cost electronic nose and a voltammetric electronic tongue for red wines identification.

The purpose of this present study was to develop a rapid and effective approach for identification of red wines that differ in geographical origins, brands, and grape varieties, a multi-sensor fusion technology based on a novel cost-effective electronic nose (E-nose) and a voltammetric electronic tongue (E-tongue) was proposed. The E-nose sensors was created using porphyrins or metalloporphyrins, pH indicators and Nile red printed on a C2 reverse phase silica gel plate. The voltammetric E-Tongue with six metallic working electrodes, namely platinum, gold, palladium, tungsten, titanium, and silver was employed to sense the taste of red wines. Principal component analysis (PCA) was utilized for dimensionality reduction and decorrelation of the raw sensors datasets. The fusion models derived from extreme learning machine (ELM) were built with PCA scores of E-nose and tongue as the inputs. Results showed superior performance (100% recognition rate) using combination of odor and taste sensors than individual artificial systems. The results suggested that fusion of the novel cost-effective E-nose created and voltammetric E-tongue coupled with ELM has a powerful potential in rapid quality evaluation of red wine.

Dynamic characteristics of dough during the fermentation process of Chinese steamed bread.

The fermentation process is crucial to the production of Chinese steamed bread (CSB). In order to select suitable indicators as the basis for further research of establishing intelligent monitoring method for dough fermentation state, this study investigated the dynamic characteristics of dough during fermentation. Indicators included water mobility and distribution, starch-pasting properties, content of free amino acid (FAA), volatile organic compounds (VOCs) and electronic nose (E-nose) response value. Starch-pasting properties of dough and relaxation time (T21, T22) did not change significantly during the fermentation process (p < 0.05). The VOCs and FAAs of the dough had significant differences (p < 0.05) in different fermentation times, but no rule was established. The E-nose response value to headspace was most suitable to monitor the fermentation of dough. Principal component analysis (PCA) was performed on E-nose data from 75 samples and the results indicated that samples of different fermentation states were accurately classified.