The way forward to produce nutraceuticals from agri-food processing residues: obstacle, solution, and possibility.

Food matrices contain bioactive compounds that have health benefits beyond nutritional value. The bulk of bioactive chemicals are still present in agro-industrial by-products as food matrices. Throughout the food production chain, there is a lot of agro-industrial waste that, if not managed effectively, could harm the environment, company, and how nutritiously and adequately people eat. It's important to establish processes that maximise the use of agro-industrial by-products, such as biological technologies that improve the extraction and acquisition of bioactive compounds for the food and pharmaceutical industries. As opposed to nonbiological processes, biological procedures provide high-quality, bioactive extracts with minimum toxicity and environmental impact. Fermentation and enzymatic treatment are biological processes for obtaining bioactive compounds from agro-industrial waste. In this context, this article summarises the principal bioactive components in agro-industrial byproducts and the biological methods employed to extract them. In this review efficient utilization of bioactive compounds from agro-industrial waste more effectively in food and pharmaceutical industries has been described.

Isolation of Food Grade Dye from Flower Petals of Butea monosperma and Determination of Marker Compounds for Its Quantitative Analysis.

Health concerns associated with synthetic dyes/colorants have fostered the use of natural coloring materials for food applications. This study has been carried out to extract a natural dye from the flower petals of Butea monosperma (family Fabaceae) under an eco-friendly and organic solvent-free approach. Hot aqueous extraction of dry B. monosperma flowers followed by lyophilization of the resulting extract furnished an orange-colored dye in ∼35% yield. Silica gel column chromatography of dye powder resulted in the isolation of three marker compounds, viz. iso-coreopsin (1), butrin (2), iso-butrin (3) which were characterized by spectral methods, e.g., ultra violet, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, and high-resolution mass spectrometry. The XRD analysis of isolated compounds established an amorphous nature for compounds 1 and 2 while compound 3 showed good crystallinity. The stability of dye powder and the isolated compounds 1-3 was determined by thermogravimetric analysis which showed excellent stability up to 200 °C. In trace metal analysis, the product B. monosperma dye powder exhibited low relative abundance <4% for Hg along with negligible concentrations of Pb, As, Cd, and Na. The detection and quantification of marker compounds 1-3 in the B. monosperma flower extracted dye powder were carried out by a highly selective UPLC/PDA method of analysis.

Isolation, optimized extraction, and ultra-high performance liquid chromatography with photodiode array method for quantitative analysis of chiratol in Swertia paniculata.

The global natural product-based industry is growing fast with the introduction of new phytochemicals and herbal extract products from different geographical regions. Swertia paniculata is a well-known plant with medicinal properties; however, the quality control for its major phytochemical constituents from the Himalayan geographical region is nevertheless reported. Therefore, the first objective of this investigation was to characterize and optimize the extraction process while the second objective was to validate a quantitative analytical method for chiratol from S. paniculata herbal extract. The chiratol was characterized with spectral analysis. The optimum extraction condition for the highest yield of metabolite was realized in chloroform as a solvent system under ultrasonication. The ultra-high performance liquid chromatography coupled with photodiode array detection method for analytical quantification was validated for specificity, linearity, limits of detection, limits of quantification, precision, repeatability, recovery, and robustness using Eclipse Plus C18 column (100 mm × 4.6 mm × 3.5 µm id). The gradient elution of water/acetonitrile as mobile phase was used at a flow rate of 0.5 ml/min. The recovery percentage was very satisfactory with values within specification. The robustness parameters showed no substantial influence of evaluated parameters by the Youden test. The developed method was ascertained to be appropriate for the proposed purpose.

Sustainable blueberry waste recycling towards biorefinery strategy and circular bioeconomy: A review.

Waste valorization using biological methods for value addition as well as environmental management is becoming popular approach for sustainable development. The present review addresses the availability of blueberry crop residues (BCR), applications of this feedstock in bioprocess for obtaining range of value-added products, to offer economic viability, business development and market potential, challenges and future perspectives. To the best of our knowledge, this is the first article addressing the blueberry waste valorization for a sustainable circular bioeconomy. Furthermore, it covers the information on the alternative BCR valorization methods and production of biochar for environmental management through removal or mitigation of organic and inorganic pollutants from contaminated sites. The review also discusses the ample opportunities of strategic utilization of BCR to offer solutions for environmental sustenance, covers the emerging trends to produce multi-products and techno-economic prospective for sustainable agronomy.

Recent developments on solid-state fermentation for production of microbial secondary metabolites: Challenges and solutions.

Microbial secondary metabolites (SMs) are the intermediate or the product of metabolism produced during fermentation process. SMs are produced during stationary phase and play a major role in competition, antagonism and self defence mechanisms. These metabolites finds application in the pharmaceuticals, food, cosmetics etc. These are produced besides primary key metabolites (e.g., amino acids, lipids, carbohydrates etc.). Growth condition in solid-state fermentation (SSF) resembles microorganism's own native environment allowing the microorganisms to adapt best. Recent developments in bioprocessing has identified specific SSF practices that have a significant impact on SMs production. The practice of SSF, representing new opportunities to design better bioprocessing with potential genetic development goals for expanding the list of exciting SMs. Current updates cover advanced techniques on SSF to improve microbial SMs production and their ease of operation and cost-effective production strategies. Various factors affecting the SSF have been discussed with respect to sustainable development of novel SSF strategies for SMs production.

A critical review on current strategies and trends employed for removal of inhibitors and toxic materials generated during biomass pretreatment.

The main objective of biomass pretreatment is to separate biomass components and provide easier access with ultimate aim for lignin removal, hemicellulose protection and cellulose crystallinity reduction. Effective bioconversion with least inhibitory compound production would play a considerable role in economic practicability of the process in order to achieve economic sustainability. In this regard, detoxification is an important condition to make biomass hydrolysate acquiescent to bioconversion; also, understanding of inhibitors effect on growth and fermentation are necessary requirements for system detoxification. A number of physical, chemical and biological methods like feedstock selection, membrane selection, neutralization, use of activated charcoal etc have been recommended and developed for removal or minimizing the inhibitory compounds effect. This work reviews various inhibitory compounds produced during pretreatment methods and their removal by various processes.

Improved upstream processing for detoxification and recovery of xylitol produced from corncob.

This work deals with the development of an improved process for xylitol production from corn cob hydrolysate by biotechnological routes emphasizing the detoxification of corncob acid hydrolysate. The acid hydrolysate obtained by acid hydrolysis of corn cob was concentrated and detoxified by activated charcoal, membrane process and ion exchange resin process. The resultant partially purified corncob hydrolysate was used in fermentation. The fermentation of acid hydrolysate containing 56.5 g/L xylose was carried out in a 14 L fermenter at pH 4.5 for 48 h with 150 rpm stirring rate at 30 °C. A xylitol yield of 62% was achieved from the partially purified acid hydrolysate medium during fermentation using Candida tropicalis MTCC 6192. The purity of xylitol was increased to 92-94% upon downstream processing of carbonation, subsequently ion exchange process and activated charcoal.

Delivery of phytochemicals by liposome cargos: recent progress, challenges and opportunities.

Bio-availability is a major concern in delivery of dietary phytochemicals for better bio-efficacy. The reduced bio-availability of food bioactive compounds is evident due to degradation during human digestion process which involves liberation, absorption, distribution, metabolism and elimination. The bio-efficacy of any nutrient can be increased by increasing bio-availability. Different technologies are available for engineered efficient delivery systems; still many challenges remain with advancement of delivery systems. The ease of preparedness and adaptability of liposomes has resulted in wide-range of applicability and acceptability in scientific field, especially as delivery vehicles. In view, of properties like biocompatibility and biodegradability, liposomes have been modified with different usable methodologies for delivery of phytochemicals. The aim of this review is to abridge liposomes, methods of preparation, their application as delivery cargo in dietary phytochemicals, result of using different preparation techniques on properties.

Isolation of a new flavonoid and waste to wealth recovery of 6-O-Ascorbyl Esters from Seeds of Aegle marmelos (family- Rutaceae).

Aegle marmelos is a plant species native to India. Commercially available food products such as jam, jelly, candy, squash etc. are prepared from ripe fruit pulp of A. marmelos. Ripe fruit processing accounts for 60% of whole fruit mass while 40% remains unutilized and generates waste (hard shell, pomace, fiber and seeds) on a massive scale which do not have high value applications. A new flavone 3,5,7-trihydroxy-2-(4'-hydroxy-3'-isopentyloxyphenyl-4H-chromen-4-one (5) in addition to the known compounds 1-4, has been isolated from seeds of A. marmelos. Also, compound 7-(3'-methylbut-2'-enyloxy)-2H-chromen-2-one (2) has been isolated for the first time from A. marmelos. The structure of compounds 1-5 was determined by spectral analysis (UV, IR, NMR, etc.). Additionally, the non-edible oil obtained from seeds was investigated for waste to wealth recovery of 6-O-ascorbyl esters in high regioselectivity via one step semi-synthetic approach in the presence of ascorbic acid and H2SO4 at ambient temperature.

Bioconversion of pentose sugars to value added chemicals and fuels: Recent trends, challenges and possibilities.

Most of the crop plants contain about 30% of hemicelluloses comprising D-xylose and D-arabinose. One of the major limitation for the use of pentose sugars is that high purity grade D-xylose and D-arabinose are yet to be produced as commodity chemicals. Research and developmental activities are going on in this direction for their use as platform intermediates through economically viable strategies. During chemical pretreatment of biomass, the pentose sugars were generated in the liquid stream along with other compounds. This contains glucose, proteins, phenolic compounds, minerals and acids other than pentose sugars. Arabinose is present in small amounts, which can be used for the economic production of value added compound, xylitol. The present review discusses the recent trends and developments as well as challenges and opportunities in the utilization of pentose sugars generated from lignocellulosic biomass for the production of value added compounds.

Efficient detoxification of corn cob hydrolysate with ion-exchange resins for enhanced xylitol production by Candida tropicalis MTCC 6192.

The present study demonstrates utilization of secondary agricultural wastes for xylitol production. The highest xylan-to-xylose (70%) conversion was achieved using dilute nitric acid as catalyst followed by resin treatment. Results show that resin treatment efficiently removed nitrate salt (70%), phenolic content and 5-HMF (70%). Highest xylitol yield (85%) was achieved during fermentation using Candida tropicalis MTCC 6192 from the neutralized hemicellulosic hydrolysate medium. Good recovery (>15%) was achieved from corncob with 85% xylose to xylitol conversion during fermentation. This two-step process for transformation of agri-waste to xylitol is much simpler and it could possibly be considered for up scaling after process optimization parameters.

Improved levulinic acid production from agri-residue biomass in biphasic solvent system through synergistic catalytic effect of acid and products.

In this study, levulinic acid (LA) was produced from rice straw biomass in co-solvent biphasic reactor system consisting of hydrochloric acid and dichloromethane organic solvent. The modified protocol achieved a 15% wt LA yield through the synergistic effect of acid and acidic products (auto-catalysis) and the designed system allowed facile recovery of LA to the organic phase. Further purification of the resulting extractant was achieved through traditional column chromatography, which yielded a high purity LA product while recovering ∼85% wt. Upon charcoal treatment of the resultant fraction generated an industrial grade target molecule of ∼99% purity with ∼95% wt recovery. The system allows the solvent to be easily recovered, in excess of 90%, which was shown to be able to be recycled up to 5 runs without significant loss of final product concentrations. Overall, this system points to a method to significantly reduce manufacturing cost during large-scale LA preparation.

Nano silver particle synthesis using Swertia paniculata herbal extract and its antimicrobial activity.

In the present study, green synthesis of silver nanoparticles (AgNPs) is demonstrated using medicinal herb Swertia paniculata extract. The plant extract acted both as reducing and capping agents during synthesis process, where silver nitrate was used as silver source. Subsequent analysis revealed that particles had size range between 31 and 44 nm and were spherical in shape. Among reaction parameters, temperature and time had significantly influenced the synthesis reaction. Also, synthesized nanoparticles were found stable up to 90 days. Further, antimicrobial activity against gram negative and gram positive bacterial strains was done and results showed that synthesized AgNPs had better antimicrobial activity against Pseudomonas aeruginosa, Klebsiella pneumoniae under standard incubation conditions. Study shows that these particles can be very promising in biomedical applications in future.

Antifungal and phytotoxic activity of essential oil from root of Senecio amplexicaulis Kunth. (Asteraceae) growing wild in high altitude-Himalayan region.

This work was aimed to evaluate the essential oil from root of medicinally important plant Senecio amplexicaulis for chemical composition, antifungal and phytotoxic activity. The chemical composition analysed by GC/GC-MS showed the presence of monoterpene hydrocarbons in high percentage with marker compounds as α-phellandrene (48.57%), o-cymene (16.80%) and ß-ocimene (7.61%). The essential oil exhibited significant antifungal activity against five phytopathogenic fungi, Sclerotium rolfsii, Macrophomina phaseolina, Rhizoctonia solani, Pythium debaryanum and Fusarium oxysporum. The oil demonstrated remarkable phytotoxic activity in tested concentration and significant reduction in seed germination percentage of Phalaris minor and Triticum aestivum at higher concentrations. The roots essential oil showed high yield for one of its marker compound (α-phellandrene) which makes it important natural source of this compound.

Comparative evaluation of two Trichoderma harzianum strains for major secondary metabolite production and antifungal activity.

This investigation was undertaken to identify the major secondary metabolite, produced by two Trichoderma harzianum strains (T-4 and T-5) with their antifungal activity against phytopathogenic fungi using poison food technique. The ethyl acetate extract was subjected to column chromatography using n-hexane, ethyl acetate and methanol gradually. Chromatographic separation of ethyl acetate extract of T. harzianum (T-4) resulted in the isolation and identification of palmitic acid (1), 1,8-dihydroxy-3-methylanthraquinone (2), 6-pentyl-2H-pyran-2-one (3), 2(5H)-furanone (4), stigmasterol (5) and ß-sitosterol (6), while T. harzianum (T-5) gave palmitic acid (1), 1-hydroxy-3-methylanthraquinone (7), δ-decanolactone (8), 6-pentyl-2H-pyran-2-one (3), ergosterol (9), harzianopyridone (10) and 6-methyl-1,3,8-trihydroxyanthraquinone (11) as major metabolites. Among compounds screened for antifungal activity, compound 10 was found to be most active (EC50 35.9-50.2 µg mL(-1)). In conclusion, the present investigation provided significant information about antifungal activity and compounds isolated from two different strains of T. harzianum obtained from two different Himalayan locations.

Chemical composition and antifungal activity of Artemisia nilagirica essential oil growing in northern hilly areas of India.

Essential oil extracted from aerial parts of Artemisia nilagirica was analysed by gas chromatography-mass spectroscopy. Forty-three constituents amounting to 98.16% of the total essential oil contents were identified. The essential oil contained approximately 79.91% monoterpenoids and 18.25% sesquiterpenoids. α-Thujone (36.35%), ß-thujone (9.37%), germacrene D (6.32%), 4-terpineol (6.31%), ß-caryophyllene (5.43%), camphene (5.47%) and borneol (4.12%) were identified as the major constituents. The essential oil exhibited significant antifungal activity against Rhizoctonia solani (ED(50), 85.75 mg L(-1)), Sclerotium rolfsii (ED(50), 87.63 mg L(-1)) and Macrophomina phaseolina (ED(50), 93.23 mg L(-1)). This study indicated that A. nilagirica essential oil can be used to control phytopathogenic fungi infesting agricultural crops and commodities.

Activity of alkanediol alkanoates against pathogenic plant fungi Rhizoctonia solani and Sclerotium rolfsii.

Thirty known dialkanoates of ethylene, propylene and diethylene glycols were synthesized by reacting the glycols with acyl chlorides and their structures confirmed by IR, NMR and mass spectral analyses. They exhibited significant antifungal activity against two phytopathogenic fungi Rhizoctonia solani Kuehn and Sclerotium rolfsii Sacc in a dose dependent manner. Propylene glycol dipentanoate was the most active against R. solani. followed by diethylene glycol dibutanoate and ethylene glycol dibutanoate. Against S. rolfsii ethylene glycol diheptanoate was found to be most active followed by diethylene glycol diisobutanoate As compared to the standard reference benomyl (EC50 5.16 microg/mL), the potential alkanediol dialkanoates showed EC50 in the range of 33 - 60 microg/mL.

Synthesis, antifungal activity and structure-activity relationships of vanillin oxime-N-O-alkanoates.

Vanillin oxime-N-O-alkanoates were synthesized following reaction of vanillin with hydroxylamine hydrochloride, followed by reaction of the resultant oxime with acyl chlorides. The structures of the compounds were confirmed by IR, 1H, 13C NMR and mass spectral data. The test compounds were evaluated for their in vitro antifungal activity against three phytopathogenic fungi Macrophomina phaseolina, Rhizoctonia solani and Sclerotium rolfsii by the poisoned food technique. The moderate antifungal activity of vanillin was slightly increased following its conversion to vanillin oxime, but significantly increased after conversion of the oxime to oxime-N-O-alkanoates. While vanillin oxime-N-O-dodecanoate with an EC50 value 73.1 microg/mL was most active against M. phaseolina, vanillin oxime-N-O-nonanoate with EC50 of value 66.7 microg/mL was most active against R. solani. The activity increased with increases in the acyl chain length and was maximal with an acyl chain length of nine carbons.

Synthesis and pesticidal activity of new N-alkyl-N-[1-(2-hydroxyphenyl) ethyl]amines.

A series of novel N-alkyl-N-[1-(2-hydroxyphenyl) ethyl]amines were synthesized as potential new agents to control pests. Their structures were confirmed on the basis of IR, NMR and elemental analyses. Six new N-alkyl-N-[1-(2-hydroxyphenyl) ethyl]amines were prepared by reduction of corresponding Schiff bases using sodium borohydride in 80-87 % yields. These compounds were tested for their antifungal activity against two pathogenic fungi viz., Rhizoctonia bataticola ITCC 0482 and Sclerotium rolfsii ITCC 5226 and for insecticidal activity against insects of stored grain pest Callosobruchus analis. Fungicidal bioassay revealed that compound N-Decyl-N-[1-(2-hydroxyphenyl)ethyl]amine, was highly effective against R. bataticola (ED(50) 6.86 mg L(-1)) which was comparable with that of commercial fungicide hexaconazole (ED(50) 6.35 mg L(-1)). Also compounds N-Heptyl-N-[1-(2-hydroxyphenyl)ethyl]amine, N-Octyl-N-[1-(2-hydroxyphenyl)ethyl]amine and N-Nonyl-N-[1-(2-hydroxyphenyl)ethyl]amine displayed promising fungitoxicity against same pathogen. However, compound N-Heptyl-N-[1-(2-hydroxyphenyl)ethyl]amine was also found to be effective against S. rolfsii (ED(50) 4.92 mg L(-1) as against 1.27 mg L(-1) for hexaconazole). Compound N-Hexyl-N-[1-(2-hydroxyphenyl)ethyl]amine was most effective as insecticide followed by compound N-Octyl-N-[1-(2-hydroxyphenyl)ethyl]amine. LC(50) values for these compounds were 155.0 and 275.0 mg L(-1) respectively as against 36.70 mg L(-1) for commercial insecticide dichlorovos. The results obtained from bioassays indicate that this class of compounds can be utilized for the design of new substances endowed with pesticidal activities.