[Study of residue preservatives thiabendazole, o-phenylphenol and diphenyl in fruits and vegetables by SPE-separation technology].

The residue of thiabendazole, o-phenylphenol and diphenyl in vegetables and fruits was detected by solid-phase extraction and ultraviolet-spectrophotometry. Samples were extracted under basic conditions with petroleum ether: ethyl acetate (2:1). The analytes were first enriched, purified and separated through a C18 solid-phase extraction column. Thiabendazole, o-phenylphenol and diphenyl in the C18 solid-phase extraction column were eluted with 30% ethanol-acid solution (pH 2.5), 55% methanol -alkaline solution (pH 11.5) and 75% ethanol-acid solution (pH 2.5) respectively ,then detected by ultraviolet-spectrophotometry. The linear ranges were from 1 to 10 microg x mL(-1) with a good linear relationship (r > 0.9998) for thiabendazole, o-phenylphenol and diphenyl. The recovery range was from 72.1% to 103.5%, with the relative standard deviations between 1.2% and 7.7%. The limit of detection (S/N = 3) was 0.09 Mg x mL(-1) (TBZ), 0.5 microg x mL(-1) (OPP) and 0.1 microg x mL(-1) (DP). The method was successfully applied to residues of preservatives in fruits and vegetables. These results indicated that this method is simple, rapid and sensitive for the simultaneous determination requirements of residues in vegetables and fruits.

Effect of Qizil Guliqent on gastrointestinal hormone in rats / 中国基层医药

ObjectiveTo investigate the effect of Qizil guliqent on gastrointestinal hormone in normal rats.MethodsThe rats in experimental groups were administered with Qizil guliqent(4g/kg,6g/kg,8g/kg ),madinglin (0.0027g/kg),and the rats in normal control group were given 0.5％ CMC-Na for twenty days.The method ELISA was used to detect the content of gastric motility(MTL),vasoactive intestinal peptide(VIP) and Gastrin(GAS) in serum of normal rats.ResultsIn comparison with normal control group,VIP increased in Qizil guliqent group(4g/kg,6g/kg) (P ＜0.05 ～P ＜ 0.01 );but MTL,GAS were not significantly changed in this group( P ＞ 0.05 ).MTL,GAS were increased in Qizil guliqent group ( 8g/kg ) and madinglin group (0.0027g/kg) ( P ＜ 0.05 ) ; but VIP was not significantiy changed in this group(P ＞ 0.05 ).ConclusionQizil guliqent could regulate gastrointestinal hormones in rats,and dose related.

[New neolignan from seed of Myristica fragrans].

OBJECTIVE: To study the chemical constituents in the nutmeg (seed of Myristica fragrans). METHOD: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, NMR and MS evidences. RESULT: Fifteen compounds were obtained and identified as myristicin (1), methyleugenol (2), safrole (3), 2, 3-dihydro-7-methoxy-2(3, 4-methylenedioxyphenyl)-3-methyl-5-(E) -propenyl-benzofuran (4), dehydrodiisoeugenol (5), 2, 3-dihydro-7-methoxy-2-(3-methoxy-4, 5-methylenedioxyphenyl) -3-methyl-5-(E)-propenyl-benzofuran (6), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4-dimetho- xyphenyl) propane (7), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4, 5-trimethoxyphenyl) propane (8), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4-dimethoxyphenyl) propan-1-ol acetate (9), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4-dimethoxyphenyl) propan-1-ol (10), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3, 4, 5-trimethoxyphenyl) propan-1-ol (11), 5-methoxy-dehydrodiisoeugenol (12), erythro-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(4-hydroxy-3-methoxyphenyl)-propan-1-ol (13), guaiacin (14) and threo-2-(4-allyl-2, 6-dimethoxyphenoxy)-1-(3-methoxy-5-hydroxy-phenyl) propan-1-ol (15). CONCLUSION: Compound 15 is a new compound and named myrisisolignan. Compound 7 is isolated from the genus Myristica for the first time.