Influence of airborne particle abrasion on dentin bonding effectiveness of a 2-step universal adhesive.

OBJECTIVE: To determine the effect of airborne particle abrasion (APA) on micro-tensile bond strength (µTBS) to dentin using different air-abrasion/polishing powders. METHODS: The bonding effectiveness of G2 Bond Universal (G2B), used in etch-and-rinse (E&R) and self-etch mode (SE), was tested on bur-cut dentin and dentin air abraded/polished using six different powders (aluminum oxide 29 µm (AO29) and 53 µm (AO53), aluminum trihydroxide (AT), sodium bicarbonate (SB), sodium bicarbonate soft (SBsoft) and bioactive glass (BG); Velopex). Adhesive-composite resin specimens were immersed in distilled water at 37 °C for one week and cut into microspecimens. Half of the specimens were subjected to 50,000 thermocycles (aged). Immediate and aged µTBS to dentin were measured. Statistical analysis was performed using linear mixed-effects (LME) modeling (p < 0.05). RESULTS: Comparing the aged bond strengths to air-abraded/polished dentin with bur-cut dentin, pretreatment with SB and SBsoft in combination with G2B used in E&R mode, and BG air polishing in combination with both application modes (E&R, SE), resulted in a significantly higher bond strength. Dentin bond strength was only significantly lower when air abraded with AO29 and using G2B in SE mode. Aging did not significantly influence bond strength for both application modes (E&R, SE), except for AO29 and AT-treated dentin, where bond strengths decrea sed significantly using G2B in SE mode. In general, G2B reached significantly higher bond strengths on air-abraded/polished dentin in E&R mode than in SE mode. CONCLUSION: Air-abrasion/polishing did not impair dentin bond strength using G2B, except when dentin was air abraded with AO29 and using G2B in SE mode. Air polishing positively influenced the bond strength to dentin in specific groups. CLINICAL SIGNIFICANCE: APA is safe concerning bonding to dentin. The E&R application mode is preferred using G2B as adhesive on air-abraded/polished dentin. Air polishing with BG positively influenced dentin bond strength for both application methods.

Association of free testosterone, glucose level and obesity among women with polycystic ovary syndrome in Soran city, Kurdistan-Iraq / Asociación de testosterona libre, nivel de glucosa y obesidad entre mujeres con síndrome de ovario poliquístico en la ciudad de Soran, Kurdistán-Irak

Background: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy affecting approximately 5–10% of women of reproductive age and it is also a major cause of anovulatory infertility. PCOS is associated with obesity and conditions like hirsutism, acne, diabetes, and irregular periods. Aim: The present study aimed to evaluate the serum-free testosterone (FT) levels of women afflicted with hirsutism, one of the main physical manifestations of PCOS versus healthy women and determine whether their serum testosterone levels correlate with polycystic ovaries, glucose levels, menstrual abnormalities, and obesity. Methods: This study assessed 180 women; this included 140 females who suffered from excessive and unwanted hair growth on the chin and 40 healthy women as a control group. Free testosterone levels and fasting blood glucose levels were taken. Prior to the study, ultrasonographic (US) tests were performed for all patients to diagnose polycystic ovaries. Results: Patients with hirsutism exhibited a significant elevation in free testosterone (FT) compared to the control group. Approximately half of these women were confirmed to have a polycystic ovary, and 42.8% of them were overweight and obese. Additionally, women between 18 and 20 years old (G1) present with the highest level of FT. Conclusion: Serum FT levels were significantly increased in hirsute women, and this positively correlated with BMI and glucose levels in women with PCOS. Glucose levels may serve as a potentially effective biomarker in evaluating the severity of hirsutism in women suspected of having PCOS.(AU)

Antecedentes: El síndrome de ovario poliquístico (SOP) es la endocrinopatía más común que afecta aproximadamente al 5-10% de las mujeres en edad reproductiva y también es una de las principales causas de infertilidad anovulatoria. El SOP está asociado con la obesidad y condiciones como hirsutismo, acné, diabetes y períodos irregulares. Objetivo: El presente estudio tuvo como objetivo evaluar los niveles séricos de testosterona libre (FT, del inglés) de mujeres con hirsutismo, una de las principales manifestaciones físicas del síndrome de ovario poliquístico, en comparación con mujeres sanas y determinar si los niveles séricos de testosterona se correlacionan con ovarios poliquísticos, niveles de glucosa, anomalías menstruales y obesidad. Métodos: Este estudio evaluó a 180 mujeres; esto incluyó a 140 mujeres que sufrían de un crecimiento de vello excesivo e indeseado en la barbilla y 40 mujeres sanas como grupo de control. Se midieron el nivel de FT y el nivel de azúcar en sangre en ayunas. Antes del estudio, se realizaron pruebas ultrasonográficas a todas las pacientes para diagnosticar ovarios poliquísticos. Resultados: Los pacientes con hirsutismo exhibieron una elevación significativa en la FT en comparación con el grupo de control. Se confirmó que aproximadamente la mitad de estas mujeres tenían un ovario poliquístico y el 42,8% de ellas tenían sobrepeso y obesidad. Adicionalmente, las mujeres entre 18 y 20 años (G1) presentan el mayor nivel de FT. Conclusión: Los niveles séricos de FT aumentaron significativamente en mujeres hirsutas, y esto se correlacionó positivamente con el IMC y los niveles de glucosa en mujeres con SOP. Los niveles de glucosa pueden servir como un biomarcador potencialmente eficaz para evaluar la gravedad del hirsutismo en mujeres con sospecha de SOP.(AU)

Overview of structural, electronic, elastic, thermal, optical, and nuclear properties of Zr2AC (A= Al, Si, P, S, Ge, As, Se In, Sn, Tl, and Pb) MAX phases: A brief review.

The Zr2AC MAX phases are a family of ternary carbides ceramics that possess layered structures and exhibiting exceptional properties resulting from combining the most desirable features of metals and ceramics. In addition, the Zr2AC MAX-phases exhibit numerous physical and chemical properties due to their chemical and structural characteristics, a tendency for multiple basal dislocations and exhibiting mobility under ambient conditions. This review extensively analyzes the properties of the Zr2AC MAX phase, as they are closely linked to the exceptional and potential applications of the MAX phase. For the first time, the present study analyzed various properties of Zr2AC MAX phases, including structural, electronic, elastic, thermal, optical, self-healing, nuclear, oxidation, and corrosion characteristics. Furthermore, this review included experimental and theoretical work with comparison. It's found that the Zr2AC lattice parameters a and c are deviations theoretically from 0.1 to 2% and 0.15-2.87% compared with experimental work. Also, the Zr2AC MAX phases are metallic characters and the conductivity differs depending on the type of the Zr2AC(different A element) MAX phases. Its concluded that the Zr2AC MAX phases are stiff, isotropic elastic properties and high machinability with damage tolerance and hardness levels ranging from 3.5 to 13.02 Gpa. The Zr2AC MAX phases are also resistant to corrosion, thermal shock, and oxidation as well as lightweight. In addition, at elevated temperatures the transition from brittle to plastic behavior can be occurred in the Zr2AC MAX phase. The Zr2AC MAX phase's optical properties are anisotropic such as electrical conductivity and mechanical properties. This review study provides a comprehensive details assisting researches to deal with Zr2AC MAX phase potentially for different applications.

Engineered Interleaved Random Glass Fiber Composites Using Additive Manufacturing: Effect of Mat Properties, Resin Chemistry, and Resin-Rich Layer Thickness.

Standard lay-up fabrication of fiber-reinforced composites (FRCs) suffer from poor out-of-plane properties and delamination resistance. While advanced manufacturing techniques (e.g., interleaving, braiding, and z-pinning) increase delamination resistance in FRCs, they typically result in significant fabrication complexity and limitations, increased manufacturing costs, and/or overall stiffness reduction. In this work, we demonstrate the use of facile digital light processing (DLP) technique to additively manufacture (AM) random glass FRCs with engineered interleaves. This work demonstrates how vat photo-polymerization techniques can be used to build composites layer-by-layer with controlled interleaf material, thickness, and placement. Note that this engineering control is almost impossible to achieve with traditional manufacturing techniques. A range of specimens were printed to measure the effect of interleaf thickness and material on tensile/flexural properties as well as fracture toughness. One important observation was the ≈60% increase in interlaminar fracture toughness achieved by using a tough resin material in the interleaf. The comparison between AM and traditionally manufactured specimens via vacuum-assisted resin transfer molding (VARTM) highlighted the limitation of AM techniques in achieving high mat consolidation. In other words, the volume fraction of AM parts is limited by the wet fiber mat process, and engineering solutions are discussed. Overall, this technique offers engineering control of FRC design and fabrication that is not available with traditional methods.

Bonding performance of experimental HEMA-free two-step universal adhesives to low C-factor flat dentin.

OBJECTIVES: Experimental two-step universal adhesives (2-UAs) providing a particle-filled hydrophobic adhesive resin with a significant film thickness to hydrophobically seal the adhesive interface were designed and synthesized. This study aimed to characterize their interfacial interaction with dentin, to determine whether the 2-UA formulations achieve durable bonding to low C-factor flat dentin and to measure their water sorption. METHODS: Bonding effectiveness of 2-UAs that combine a 10-MDP-based primer with hydrophobic adhesive resins differing only for filler (BZF-21, BZF-29, and BZF-29_hv) were comparatively investigated with the commercial adhesive Clearfil SE Bond 2 (C-SE2, Kuraray Noritake). Adhesive-dentin interfaces were characterized with TEM. Adhesive-resin disks were immersed in distilled water at 37 °C for 1 week, 6 months and 1 year to measure water sorption and solubility. 'Immediate' and 'aged' micro-tensile bond strength (µTBS) of the adhesives applied in etch-and-rinse (E&R) and self-etch (SE) bonding mode to low C-factor flat dentin were measured. Statistical analyses involved linear mixed-effects (LME) modelling and Kruskal-Wallis testing (p < 0.05). RESULTS: TEM revealed that E&R hybrid layers were more sensitive to aging than SE hybrid layers. Lower water sorption was recorded for all UAs compared with C-SE2. The immediate µTBS of BZF-21 and BZF-29 was not significantly different from that of C-SE2. The 1-year aged µTBS of all 2-UAs was significantly lower than that of C-SE2, except for BZF-29 applied in E&R mode. A significant reduction in µTBS upon 1-year aging was recorded for BZF-21 and BZF-29 applied in E&R mode. A significant difference in µTBS between E&R and SE bonding modes was recorded for all adhesives except BZF-21. SIGNIFICANCE: Experimental 2-UAs with a hydrophobic adhesive-resin design produced± 20-µm thick adhesive-resin layers, absorbed less water and resulted in bonding performance that was more aging-resistant when applied in SE than in E&R bonding mode. The silica-filled BZF-29 2-UA revealed the most comparable bonding performance with C-SE2 in a low C-factor condition (flat dentin).

Overcoming the blood brain barrier in glioblastoma: Status and future perspective.

Glioblastoma is the most common primary brain malignancy in adults. Treatment of glioblastoma patients is based on neurosurgery, radiation therapy and chemotherapy. Despite this multimodal therapeutic regimen, the prognosis of glioblastoma patients is poor. Indeed, glioblastoma is very resistant to treatments due to multiple molecular and cellular mechanisms including the existence of the blood-brain barrier (BBB). The BBB consists of multiple layers surrounding brain vessels and limits drug penetration within the brain. Therefore, overcoming the BBB is a strategy to increase bioavailability and efficacy of therapeutic agents against glioblastoma cells. The development of two approaches is ongoing: i) enhancing the delivery of drugs to the brain and ii) improving the penetration of drugs into the brain. One way to enhance drug delivery to the brain is through high-dose intravenous chemotherapy, with or without bone marrow transplantation, or via intra-arterial chemotherapy, with or without disrupting the BBB through osmotic means. Conversely, improving drug penetration within the brain can be achieved through modifying either the drug itself or the BBB. Promising results in terms of safety and signals of efficacy were obtained with these approaches in early phase clinical trials. More advanced comparative clinical trials are needed to investigate the clinical benefit for glioblastoma patients.

Acrylamide monomers in universal adhesives.

OBJECTIVES: The mono-functional monomer 2-hydroxyethyl methacrylate (HEMA) is often added to universal adhesives (UAs) to improve surface wetting and prevent phase separation. Nevertheless, HEMA promotes water sorption and hydrolysis at adhesive interfaces, hereby affecting long-term bonding to dentin. This study investigated if two acrylamide monomers could replace HEMA in an UA formulation applied in etch-and-rinse (2E&R) and self-etch (1SE) bonding mode. METHODS: Four experimental UAs were bonded to bur-cut dentin. In addition to 12 wt% 10-MDP, 25 wt% Bis-GMA and 10 wt% TEGDMA as common monomer composition, 20 %wt ethanol and 15 %wt water as solvent, and 3 wt% polymerization-related additives, the four formulations solely differed for either the acrylamide cross-linker monomer 'FAM-201' as TEGDMA alternative and HEMA replacement, the hydroxyethyl acrylamide monomer 'HEAA' as HEMA alternative, HEMA ('HEMA+'), or extra TEGDMA in a HEMA-free control ('HEMA-'), all added in a 15 wt% concentration. The split-tooth study design involved application in 2E&R mode on one tooth half versus 1SE mode on the corresponding half. Micro-tensile bond strength of half of the micro-specimens was measured upon 1-week distilled water storage ('immediate' 1w µTBS), with the other half measured after additional 6-month storage ('aged' 6 m µTBS). Statistics involved linear mixed-effects (LME) modelling (p < .05). Additionally, interfacial TEM characterization, thin-film (TF) XRD surface analysis, LogP determination, and a cytotoxicity assay were carried out. RESULTS: FAM-201 revealed significantly higher µTBS than HEMA+ at 1w and 6 m when applied both in E&R and SE bonding modes. HEAA's µTBS was significantly lower than that of HEMA+ at 1w when applied in SE mode. TF-XRD and TEM revealed similar chemical and ultrastructural interfacial characterization, including stable 10-MDP_Ca salt nano-layering. FAM-201 was least cytotoxic and presented with an intermediary LogP, while HEAA presented with the highest LogP, indicating high hydrophilicity and water-sorption sensitivity. SIGNIFICANCE: The acrylamide co-monomer FAM-201 could replace HEMA in an UA formulation, while HEAA not.

Synthesizinge a novel Zr2Al-GNS MAX phase ceramic with superior electrical properties using pressureless sintering technique.

A unique Zr2Al-GNS MAX phase ceramic supported nanographene sheet was prepared using a cost-effective pressureless sintering technique under relatively low temperature. An experimental investigation was conducted to explore the lattice parameters using different temperatures, such as 1000, 1150, and 1300 °C. To characterize the crystal structure of the MAX phase ceramic, X-ray diffraction, field emission scanning electron microscopy imaging, energy-dispersive X-ray spectroscopy (EDX), high-resolution transmission electron microscopy (HRTEM), and selected area diffraction (SAED) were utilized. The results revealed that the pressureless sintering technique was successfully utilized to synthesize the Zr2Al-GNS MAX phase ceramic under 1150 °C with a low impurity ratio of secondary phases such as Zr3AL2, Zr3AL5, and ZrC components. The high percentage of the Zr2Al-GNS MAX phase ceramic was obtained at 49.0% at 1150 °C compared with different temperatures. The BET surface area (SBET), pore volume, and pore size were also investigated. The SBET of the prepared Zr2Al-GNS MAX phase was increased to 30% using graphene nanosheet, while the porosity was highly decreased to 8% from its original value. The electrical properties were also studied in this research for potential applications, such as the absolute value of impedance (Z), absolute value of admittance (Y), induction (L), capacitance (C), resistance (R), conductance (G), susceptibility (B), and phase angle (Ï´). It was found that the capacitance and the phase angle were improved using the prepared Zr2Al-GNS MAX phase ceramic, depending on the frequencies. The results presented here may facilitate the improvements in the features of the MAX phase type of Zr2Al-GNS-enhanced one-layer nanographene sheet for electrical applications ceramic.

Phytochemistry and Biological Properties of Salvia verbenaca L.: A Comprehensive Review.

The family Lamiaceae contains several plants used in traditional medicine to fight against different diseases. Salvia verbenaca L. (S. verbenaca) is one of the Lamiaceae species distributed around the Mediterranean regions. This plant exhibits different bioactive properties, including antibacterial, anticancer, antioxidant, antileishmanial, antidiabetic, immunomodulatory, and wound healing. This review was conducted to revise previous studies on S. verbenaca addressing its botanical description, geographical distribution, and phytochemical, pharmacological, and toxicological properties. Moreover, the main pharmacological actions of S. verbenaca major compounds were well investigated. Literature reports have revealed that S. verbenaca possesses a pivotal role in medicinal applications. The findings of this work noted that S. verbenaca was found to be rich in chemical compound classes such as terpenoids, phenolics, fatty acids, sterols, and flavonoids. Numerous studies have found that S. verbenaca essential oils and extracts have a wide range of biological effects. These results support the potential pharmacological properties of S. verbenaca and its traditional uses. This analysis can constitute a scientific basis for further refined studies on its pure secondary metabolites. Therefore, the outcome of the present work may support the perspective of identifying new therapeutical applications with detailed pharmacological mechanisms of S. verbenaca to prevent the development of some diseases such as neurodegenerative disorders. However, toxicological investigations into S. verbenaca are needed to assess any potential toxicity before it can be further used in clinical studies.

Taxonomic Revisiting and Phylogenetic Placement of Two Endangered Plant Species: Silene leucophylla Boiss. and Silene schimperiana Boiss. (Caryophyllaceae).

The genus Silene L. is one of the largest genera in Caryophyllaceae, and is distributed in the Northern Hemisphere and South America. The endemic species Silene leucophylla and the near-endemic S. schimperiana are native to the Sinai Peninsula, Egypt. They have reduced population size and are endangered on national and international scales. These two species have typically been disregarded in most studies of the genus Silene. This research integrates the Scanning Electron Microscope (SEM), species micromorphology, and the phylogenetic analysis of four DNA markers: ITS, matK, rbcL and psb-A/trn-H. Trichomes were observed on the stem of Silene leucophylla, while the S. schimperiana has a glabrous stem. Irregular epicuticle platelets with sinuate margin were found in S. schimperiana. Oblong, bone-shaped, and irregularly arranged epidermal cells were present on the leaf of S. leucophylla, while Silene schimperiana leaf has "tetra-, penta-, hexa-, and polygonal" epidermal cells. Silene leucophylla and S. schimperiana have amphistomatic stomata. The Bayesian phylogenetic analysis of each marker individually or in combination represented the first phylogenetic study to reveal the generic and sectional classification of S. leucophylla and S. schimperiana. Two Silene complexes are proposed based on morphological and phylogenetic data. The Leucophylla complex was allied to section Siphonomorpha and the Schimperiana complex was related to section Sclerocalycinae. However, these two complexes need further investigation and more exhaustive sampling to infer their complex phylogenetic relationships.

Principles and guidelines in the management of ankle fractures in adults.

Ankle fractures are common injuries that have many physical and psychosocial complications. As a result, it is important to be aware of how these patients present and are managed perioperatively. Detailed guidelines from NICE and the British Orthopaedic Association have been produced on this topic, including recent developments such as the decision to weight-bear early after surgery and the use of virtual fracture clinics. This article provides an overview of the key perioperative factors that need to be considered in cases of ankle fracture and the relevant clinical guidelines.

Nano BEA zeolite catalysts for the selective catalytic cracking of n-dodecane to light olefins.

Nano BEA zeolite catalysts were synthesized and modified by desilication and then ion-exchanged with Co. The desilication was carried out using 0.1 M of NaOH. The synthesized and modified nano BEA catalysts were characterized via different characterization techniques. Ammonia temperature program desorption (NH3-TPD) and the pyridine Fourier transform infrared (pyridine-FTIR) were utilized to investigate the acidity of catalysts. X-ray diffraction (XRD), 27Al and 29Si nuclear magnetic resonance (NMR) spectroscopy techniques were used to examine the structure of the catalysts. The XRD patterns of the as-synthesized nano BEA catalysts were identical to that of the reference, while the NMR analysis revealed the distribution of silicon and aluminum in the BEA structure. The scanning electron microscope (SEM) analysis confirmed that the fabricated catalysts were less than 100 nm. The desilication and Co ion-exchange altered the acidity of the catalyst. The catalysts were evaluated in the cracking of sssssss to light olefins in the temperature range from 400 °C to 600 °C. The conversion increased with the increase in the reaction temperature for both catalysts; the conversion was above 90% for the Co-BEA catalyst at a temperature above 450 °C. The yield of light olefins also increased at higher temperatures for both catalysts, while at a lower temperature the yield to light olefins was ca. 40% over that of Co-BEA.

Abdominal aortic aneurysms part one: Epidemiology, presentation and preoperative considerations.

An abdominal aortic aneurysm is an irreversible dilatation of the abdominal aorta. The majority of abdominal aortic aneurysms are asymptomatic and identified incidentally while investigating a separate pathology. Others are detected by national screening programmes and some present due to a growth or rupture. Symptomatic or ruptured aneurysms require urgent or emergency repair in patients fit for surgery. Perioperative practitioners should therefore be aware of how patients with abdominal aortic aneurysms present and are investigated, so that they can implement timely management. Guidelines have been recently updated to reflect this. This literature review discusses these recommendations and explores the evidence upon which they are based. The aim of this article is to highlight the important preoperative principles that need to be considered in cases of abdominal aortic aneurysm.

An overview of the key principles and guidelines in the management of pelvic fractures.

Pelvic fractures are complex injuries with a range of different presentations depending on the mechanism of trauma. Due to the morbidity and mortality of pelvic fractures, patients require thorough investigation and timely management with multidisciplinary input. Various surgical and non-surgical techniques can be used to treat pelvic fractures, as well as any associated visceral injuries. Following repair, it is important to remain vigilant for postoperative complications such as infection, sexual and urinary dysfunction, chronic pain and adverse psychological health. This article summarises the relevant UK guidance and literature and presents them in a format that follows the patient's journey. In doing so, it highlights the key perioperative factors that need to be considered in cases of pelvic fracture.

Abdominal aortic aneurysms part two: Surgical management, postoperative complications and surveillance.

Large, symptomatic and ruptured abdominal aortic aneurysms are usually treated surgically if patients are deemed fit enough. This may be achieved through endovascular or open surgical repair. The type of treatment that a patient receives is dependant on many factors, such as the rupture status of the aneurysm. Each approach is also associated with different risks and postoperative complications. Multiple guidelines exist to inform the surgical management of abdominal aortic aneurysms. This literature review combines these recommendations and explores the evidence upon which they are based. In addition, it highlights the key perioperative considerations that need to be considered in cases of unruptured and ruptured abdominal aortic aneurysms.

Multiparameter evaluation of acrylamide HEMA alternative monomers in 2-step adhesives.

OBJECTIVE: As frequently added to adhesives, the mono-functional monomer 2-hydroxyethyl methacrylate (HEMA) acts as co-solvent and improves surface wetting. Nevertheless, HEMA promotes watersorption and hydrolysis at adhesive interfaces, affecting bond durability to dentin. This study investigated if two acrylamide co-monomer alternatives could replace HEMA in experimental adhesive-resin formulations as part of 3/2-step universal adhesives applied, respectively, in etch-and-rinse (E&R) and self-etch (SE) bonding modes. METHODS: Upon priming dentin with the 10-MDP-based Clearfil SE Bond 2' primer ('C-SE2p'; Kuraray Noritake), three experimental adhesive resins, consisting of 50 wt.% Bis-GMA, 15 wt.% TEGDMA, and either 35 wt.% diethyl acrylamide ('DEAA'), hydroxyethyl acrylamide ('HEAA') or HEMA ('HEMA+'), were applied. The control HEMA-free adhesive resin contained 60 wt.% Bis-GMA and 40 wt.% TEGDMA ('HEMA-'). All adhesives were evaluated for 'immediate' and 'aged' micro-tensile bond strength (µTBS) to dentin upon, respectively, 1-week (1w) and 6-month (6m) water storage, TEM adhesive-dentin interfacial interaction, 24-h and 6m three-point bending, contact-angle wetting, viscosity and watersorption. RESULTS: Linear mixed-effects model statistics revealed significantly better bonding performance of the adhesives applied in E&R than SE mode, except for DEAA_1w, with the highest µTBSs recorded for DEAA and HEMA- applied in SE mode. In E&R mode, aging did not significantly reduce DEAA's µTBS. Best wetting on primed dentin was recorded for HEMA+, significantly better than DEAA, further HEAA and HEMA-, these directly related to their viscosity. HEAA absorbed significantly more water than all other adhesive-resin formulations. HEMA->DEAA>HEAA>HEMA+ was the significant order for 6m bending strength. CONCLUSIONS: The acrylamide co-monomer DEAA could replace HEMA, while HEAA not.

Fluorinations of unsymmetrical diaryliodonium salts containing ortho-sidearms; influence of sidearm on selectivity.

Activated aromatics were reacted with two different fluoroidoane reagents 1 and 2 in the presence of triflic acid to prepare only the para-substituted diaryliodonium salts. With fluoroiodane 1 the unsymmetrical diaryliodonium salts contained an ortho-propan-2-ol sidearm, whereas the alcohol sidearm was eliminated to form an ortho-styrene sidearm in the reaction with fluoroiodane 2. Only the diaryliodonium salts containing a styrene sidearm were fluorinated successfully to deliver para-fluorinated aromatics in good yields.

Analysis of total phenolic compounds in tea and fruits using diazotized aminobenzenes colorimetric spots.

The present work describes a novel and rapid approach for evaluating total phenolic compounds (TPCs) in tea and fruits using colorimetric spots and the digital image-based (DIB) method. Colorimetric spots were formed by reacting diazotized aminobenzenes namely sulfanilic acid, sulfanilamide, or aniline with TPCs in the extract to form an azo dye. The limit of detection (LOD) was 6.5, 5.5, or 5.1 mg GAE (gallic acid equivalent) L-1 and the analytical range was 25-500, 20-500, or 18-200 mg GAE L-1, respectively. Correlation with the Folin-Ciocalteu assay was significant (Pearson coefficient, R = 0.970-0.991) while the antioxidant activity assay was moderate to high (R = 0.737-0.977). The method developed was successfully applied to the analysis of tea and fruits and showed RSD (n = 3) not exceeding 9.6, 8.5, and 9.7%, respectively. Ecologically, the DIB method developed could determine the variation of TPCs within cultivars and was found to be strongly dependent on the growing environment.