RESUMEN
With an aim to develop chitosan film with controllable swelling behavior and maximum antimicrobial efficacy, we hereby report cellulose nano crystals loaded chitosan films with Curcumin/Ag nano particles embedded as strong antimicrobial agents. The CNC had average size of 40-90nm with poly dispersity index of 3.641. The TEM analysis of Ag NPs, produced via DMF reduction, revealed a particle size range of 15-25nm. The Surface Plasmon resonance (SPR) of these Ag NPs shifted from 380 to 440nm, with the increase in concentration of Ag (I). The TGA of the film samples Ch/CNC, and Ch/CNC (Ag463/Cur450) exhibited% weight loss of around 95 and 80 respectively, thus indicating higher stability due to presence of Ag NPs. In XRD analysis 2θ reflections at 38.12, 44.28 and 66.46°, confirmed the presence planes (1 1), (2 0) and (2 0) respectively. In skin irritation test, the mean Erythema scores observed after 72h was zero for both the Curcumin and Ag NPs/Curcumin loaded films. The percent wound reduction, observed for the film samples Ch/CNC (Cur450) and Ch/CNC (Ag NP463/Cur450) was 57.8 and 97.2 respectively, indicating better suitability of Ag NPs/Curcumin loaded film.
Asunto(s)
Materiales Biocompatibles/farmacología , Celulosa/química , Quitosano/química , Curcumina/química , Nanopartículas del Metal/química , Plata/química , Cicatrización de Heridas/efectos de los fármacos , Animales , Vendajes , Materiales Biocompatibles/química , Femenino , Masculino , Ratas , Ratas Wistar , Piel/citología , Piel/efectos de los fármacosRESUMEN
Following the novel 'vapor induced phase inversion' (VIPI) method, we have prepared curcumin loaded chitosan/cellulose micro crystals composite films and characterized them by thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The equilibrium moisture absorption behavior of these films was investigated under different relative humidity (RH) environments and the data obtained was interpreted by the GAB isotherm model successfully. The films were also studied for their curcumin release behavior in the physiological fluid (PF) at 37 °C and the kinetic data obtained was best interpreted by Higuchi model. Finally, the films showed fair antimicrobial action against bacteria and fungi.
Asunto(s)
Antiinfecciosos/farmacología , Celulosa/química , Quitosano/química , Curcumina/farmacología , Antiinfecciosos/química , Vendajes , Candida albicans/efectos de los fármacos , Candida albicans/crecimiento & desarrollo , Cristalización , Curcumina/química , Pruebas Antimicrobianas de Difusión por Disco , Liberación de Fármacos , Escherichia coli/química , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Humedad , Cinética , Membranas Artificiales , Resistencia a la TracciónRESUMEN
A new technique, called vapor induced phase inversion (VIPI), has been employed to fabricate cellulose micro crystals (CMC)-loaded chitosan (Ch) films. The method involves immediate exposure of CMC-dispersed chitosan solution to NH3 gas. The films were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analysis. The swelling ratio (SR) of films showed negative dependence on the cellulose content in the films. The dynamic water uptake data were interpreted by various kinetic models. Finally, the release of curcumin from the films was investigated. The CMC-loaded chitosan film showed slower release as compared to the plain chitosan film, suggesting that cellulose micro crystals acted as diffusion barrier. The films were non-cytotoxic, non-thrombogenic and non-hemolytic.
