Evaluation of the Capparis Herbacea Willd's Chemistry, Antioxidant and Cytotoxic Activity.

BACKGROUND: The Capparidaceae family includes the medicinal herb Capparis herbacea Willd. The aerial and underground parts of plant C.herbacea were studied for their chemical composition, antioxidant, and cytotoxic properties. METHODS: Using gas chromatography with mass spectrometric detection (7890A/5975C), 94 chemicals were identified in ethanol extract from leaves, roots, seeds, and stems of C. herbacea. Main components were (leaves) phytol 18.16%, hexanedioic acid, bis(2-ethylhexyl) ester 16.75%, vitamin E 11.95%, (roots) sucrose 13.94%, hexadecanoic acid, ethylester 22.80%, octadecanoic acid, ethylester 37.77%; (seeds) hexadecanoic acid, ethylester 13.96%, ethyl9.cis.,11.trans.-octadecadienoate 48.54%, bis(2-ethylhexyl) phthalate 9.77%; (stems) 1-propene-1,2,3-tricarboxylic acid, tributyl ester 42.69%, and tributylacetylcitrate 19.63%. Nine components were identified in the makeup of the C. herbacea sample's essential oil using the method of chromatography-mass spectrometry. RESULTS: The main components were (in%): T-cadinol (29.56), meta-cymene (16.12), pulegone (14.11), and σ-amorphene (12.26). Chloroform and methanol extracts of Capparis herbacia roots at concentrations of 1 mg/ml showed higher average antioxidant activity, while ethyl acetate root extract at concentrations of 0.75 and 1 mg/ml showed higher average antioxidant activity compared to gallic acid AOA. CONCLUSION: In addition, plant extracts have cytotoxic activity. Essential oils of leaves and stems, fruit and roots of Capparis herbacia plants exhibited cytotoxicity, all larvae died, and larval mortality was 96%.

Development of Environmentally Clean Construction Materials Using Industrial Waste.

The accumulated waste generated from industries severely affects environmental conditions. Using waste as a construction material or soil stabilization is an emerging area in the construction industry. Introducing new additive materials to strengthen local soils using industrial waste is an inexpensive and more effective method to improve the soil. In light of this, this study aims to develop environmentally clean construction materials for stabilizing natural loam (NL) using red mud (RM), blast furnace slag (BFS), and lime production waste (LPW). Nine different mixtures were prepared with four different combinations of RM (20, 30, and 40%), BFS (25, 30 and 35%), LPW (4, 6 and 8%), and various content of NL. X-ray diffraction (XRD), X-ray fluorescence (XRF), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), atomic absorption spectroscopy (AAS), and axial compressive strength were examined. The results indicated that the optimum strength was obtained from the sample containing 40% of RM, 35% of BFS, and 8% of LPW. The observed compressive strength of the sample for 90 days was 7.38 MPa, water resistance was 7.12 MPa, and frost resistance was 7.35 MP, with low linear expansion meeting the demands for first class construction materials of the Kazakh norms. The mineral composition analysis evidenced the lack of heavy metals contaminants and hazardous compounds. Based on strength and environmental performance, RM, BFS, LPW, and NL mix can be used as a road base material. This process is believed to reduce environmental pollution related to RM and BFS, and lower the road base cost.

Insights from zebrafish and mouse models on the activity and safety of ar-turmerone as a potential drug candidate for the treatment of epilepsy.

In a previous study, we uncovered the anticonvulsant properties of turmeric oil and its sesquiterpenoids (ar-turmerone, α-, ß-turmerone and α-atlantone) in both zebrafish and mouse models of chemically-induced seizures using pentylenetetrazole (PTZ). In this follow-up study, we aimed at evaluating the anticonvulsant activity of ar-turmerone further. A more in-depth anticonvulsant evaluation of ar-turmerone was therefore carried out in the i.v. PTZ and 6-Hz mouse models. The potential toxic effects of ar-turmerone were evaluated using the beam walking test to assess mouse motor function and balance. In addition, determination of the concentration-time profile of ar-turmerone was carried out for a more extended evaluation of its bioavailability in the mouse brain. Ar-turmerone displayed anticonvulsant properties in both acute seizure models in mice and modulated the expression patterns of two seizure-related genes (c-fos and brain-derived neurotrophic factor [bdnf]) in zebrafish. Importantly, no effects on motor function and balance were observed in mice after treatment with ar-turmerone even after administering a dose 500-fold higher than the effective dose in the 6-Hz model. In addition, quantification of its concentration in mouse brains revealed rapid absorption after i.p. administration, capacity to cross the BBB and long-term brain residence. Hence, our results provide additional information on the anticonvulsant properties of ar-turmerone and support further evaluation towards elucidating its mechanism of action, bioavailability, toxicity and potential clinical application.