Genetic analysis of an interspecific cross in ornamental Viburnum (Viburnum).

A genetic analysis was performed on a population derived from crosses between Viburnum lantana and Viburnum carlesii. Linkage maps were developed for each species using AFLP, random amplified polymorphic DNA (RAPD), and sequence-tagged site markers and a half-sib approach that took advantage of both the polymorphism between the species and the heterozygosity within each parent. The map for V. lantana consisted of 153 DNA markers and spanned approximately 750 cM, whereas that for V. carlesii contained 133 markers and covered 700 cM. These maps were used to determine the location of several major genes influencing leaf spot resistance, Verticillium wilt resistance, bud color, and flower scent. Both species contained moderate levels of heterozygosity. Flow cytometric analysis revealed that the genome of V. lantana was 40% larger than that of V. carlesii, and this difference was paralleled by a proportionally greater number of intercross markers (markers segregating 3:1) from V. lantana than from V. carlesii. In addition, V. lantana (n = 9) displayed a 10th linkage group for which no homolog in V. carlesii (n = 9) could be found and which contained only markers present in the former species and absent in the latter. These results suggest that Viburnum could be an interesting genetic model for Caprifoliaceae sensu lato.

A Candida albicans early stage biofilm detachment event in rich medium.

BACKGROUND: Dispersal from Candida albicans biofilms that colonize catheters is implicated as a primary factor in the link between contaminated catheters and life threatening blood stream infections (BSI). Appropriate in vitro C. albicans biofilm models are needed to probe factors that induce detachment events. RESULTS: Using a flow through system to culture C. albicans biofilms we characterized a detachment process which culminates in dissociation of an entire early stage biofilm from a silicone elastomer surface. We analyzed the transcriptome response at time points that bracketed an abrupt transition in which a strong adhesive association with the surface is weakened in the initial stages of the process, and also compared batch and biofilm cultures at relevant time points. K means analysis of the time course array data revealed categories of genes with similar patterns of expression that were associated with adhesion, biofilm formation and glycoprotein biosynthesis. Compared to batch cultures the biofilm showed a pattern of expression of metabolic genes that was similar to the C. albicans response to hypoxia. However, the loss of strong adhesion was not obviously influenced by either the availability of oxygen in the medium or at the silicone elastomer surface. The detachment phenotype of mutant strains in which selected genes were either deleted or overexpressed was characterized. The microarray data indicated that changes associated with the detachment process were complex and, consistent with this assessment, we were unable to demonstrate that transcriptional regulation of any single gene was essential for loss of the strong adhesive association. CONCLUSION: The massive dispersal of the early stage biofilm from a biomaterial surface that we observed is not orchestrated at the level of transcriptional regulation in an obvious manner, or is only regulated at this level by a small subpopulation of cells that mediate adhesion to the surface.

Heat-tolerant flowering plants of active geothermal areas in Yellowstone National Park.

A broad survey of most of the major geyser basins within Yellowstone National Park (Wyoming, USA) was conducted to identify the flowering plants which tolerate high rhizosphere temperatures (> or = 40 degrees C) in geothermally heated environments. Under such conditions, five species of monocots and four species of dicots were repeatedly found. The predominant flowering plants in hot soils (>40 degrees C at 2-5 cm depth) were grasses, primarily Dichanthelium lanuginosum. Long-term (weeks to months) rhizosphere temperatures of individual D. lanuginosum above 40 degrees C were recorded at several different locations, both in the summer and winter. The potential role of heat shock proteins (HSPs) in the apparent adaptation of these plants to chronically high rhizosphere temperatures was examined. Antibodies to cytoplasmic class I small heat shock proteins (sHSPs) and to HSP101 were used in Western immunoblot analyses of protein extracts from plants collected from geothermally heated soils. Relatively high levels of proteins reacting with anti-sHSP antibodies were consistently detected in root extracts from plants experiencing rhizosphere temperatures above 40 degrees C, though these proteins were usually not highly expressed in leaf extracts from the same plants. Proteins reacting with antibodies to HSP101 were also present both in leaf and root extracts from plants collected from geothermal soils, but their levels of expression were not as closely related to the degree of heat exposure as those of sHSPs.