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1.
Curr Microbiol ; 74(4): 449-454, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-28213662

RESUMEN

Three sulfate-reducing bacterial strains designated SM40T, SM41, and SM43 were isolated from marine sediment in the region of Skhira located in the Gulf of Gabes (Tunisia). These strains grew in anaerobic media with phosphogypsum as a sulfate source and sodium lactate as an electron and carbon source. One of them, strain SM40T, was characterized by phenotypic and phylogenetic methods. Cells were ovoid, Gram-stain-negative and non-motile. The temperature limits for growth were 10 and 55 °C with an optimum at 35 °C and the pH range was 6.5-8.1 with an optimum at pH 7.5. Growth was observed at salinities ranging from 10 to 80 g NaCl l-1 with an optimum at 30 g NaCl l-1. Strain SM40T was able to utilize butanol, ethanol, formate, L-glucose, glycerol, lactate, propanol, propionate, and pyruvate as electron donors for the reduction of sulfate, sulfite, or thiosulfate to H2S. Without electron acceptors, strain SM40T fermented butanol and pyruvate. The DNA G+C content of strain SM40T was 52.6 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate revealed that strain SM40T was closely related to the species in the genus Desulfobulbus of the family Desulfobulbaceae. The sequence similarity between strain SM40 and Desulfobulbus marinus was 95.4%. The phylogenetic analysis, DNA G+C content, and differences in substrate utilization suggested that strain SM40 represents a new species of the genus Desulfobulbus, D. aggregans sp. nov. The type strain is strain SM40T (=DSM 28693T = JCM 19994T).


Asunto(s)
Sedimentos Geológicos/microbiología , Bacterias Reductoras del Azufre/metabolismo , Técnicas de Tipificación Bacteriana , Composición de Base/genética , ADN Bacteriano/genética , Oxidación-Reducción , Filogenia , ARN Ribosómico 16S/genética , Agua de Mar/microbiología , Bacterias Reductoras del Azufre/clasificación , Bacterias Reductoras del Azufre/genética
2.
Sci Rep ; 6: 22960, 2016 Mar 11.
Artículo en Inglés | MEDLINE | ID: mdl-26965360

RESUMEN

Microbiology of a hypersaline oil reservoir located in Central Africa was investigated with molecular and culture methods applied to preserved core samples. Here we show that the community structure was partially acquired during sedimentation, as many prokaryotic 16S rRNA gene sequences retrieved from the extracted DNA are phylogenetically related to actual Archaea inhabiting surface evaporitic environments, similar to the Cretaceous sediment paleoenvironment. Results are discussed in term of microorganisms and/or DNA preservation in such hypersaline and Mg-rich solutions. High salt concentrations together with anaerobic conditions could have preserved microbial/molecular diversity originating from the ancient sediment basin wherein organic matter was deposited.


Asunto(s)
Archaea/genética , Yacimiento de Petróleo y Gas/microbiología , Filogenia , ARN Ribosómico 16S/genética , África Central , Archaea/química , Magnesio/química , Yacimiento de Petróleo y Gas/química , Salinidad
3.
Extremophiles ; 20(2): 125-38, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26724953

RESUMEN

Prokaryotic diversity was investigated in a Tunisian salt lake, Chott El Jerid, by quantitative real-time PCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting methods targeting the 16S rRNA gene and culture-dependent methods. Two different samples S1-10 and S2-10 were taken from under the salt crust of Chott El Jerid in the dry season. DGGE analysis revealed that bacterial sequences were related to Firmicutes, Proteobacteria, unclassified bacteria, and Deinococcus-Thermus phyla. Anaerobic fermentative and sulfate-reducing bacteria were also detected in this ecosystem. Within the domain archaea, all sequences were affiliated to Euryarchaeota phylum. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of bacteria was 5 × 10(6) DNA copies g(-1) whereas archaea varied between 5 × 10(5) and 10(6) DNA copies g(-1) in these samples. Eight anaerobic halophilic fermentative bacterial strains were isolated and affiliated with the species Halanaerobium alcaliphilum, Halanaerobium saccharolyticum, and Sporohalobacter salinus. These data showed an abundant and diverse microbial community detected in the hypersaline thalassohaline environment of Chott El Jerid.


Asunto(s)
Lagos/microbiología , Microbiota , Salinidad , Tolerancia a la Sal , Archaea/aislamiento & purificación , Deinococcus/aislamiento & purificación , Firmicutes/aislamiento & purificación , Lagos/química , Proteobacteria/aislamiento & purificación , ARN Ribosómico 16S/genética , Thermus/aislamiento & purificación , Túnez
4.
Environ Sci Pollut Res Int ; 23(5): 4046-53, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25772882

RESUMEN

The occurrence of magnetotactic bacteria (MTB) on a Tunisian marine coast exposed to heavy metals pollution (Sfax, Gulf of Gabès, Mediterranean Sea) was investigated. The MTB population of this Southern Mediterranean coast was compared to the MTB populations previously investigated on the French Northern Mediterranean coast. A dominant MTB coccus morphotype was observed by microscopy analysis. By pyrosequencing technology, the analysis of the 16S ribosomal RNA (rDNA) revealed as much as 33 operational taxonomic sequence units (OTUs) close to sequences of MTB accessible in the databases. The majority were close to MTB sequences of the "Med group" of α-Proteobacteria. Among them, a dominant OTU_001 (99 % of the MTB sequences) affiliated within the Magnetococcales order was highlighted. Investigating the capacities of this novel bacterium to be used in bioremediation and/or depollution processes could be envisaged.


Asunto(s)
Alphaproteobacteria/crecimiento & desarrollo , Sedimentos Geológicos/microbiología , Microbiología del Agua , Alphaproteobacteria/genética , ADN Bacteriano/genética , ADN Ribosómico/genética , Sedimentos Geológicos/química , Mar Mediterráneo , Filogenia , ARN Ribosómico 16S/genética , Propiedades de Superficie , Túnez
5.
Int J Syst Evol Microbiol ; 65(Pt 2): 543-548, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25389147

RESUMEN

Halophilic, obligately anaerobic, Gram-stain-negative bacterial strains were isolated from a sediment sample taken from under the salt crust of El-Jerid hypersaline lake in southern Tunisia by using tryptone or glucose as the substrate. One strain, CEJFT1B(T), was characterized phenotypically and phylogenetically. Cells were non-motile, non-spore-forming, short rods. Strain CEJFT1B(T) was able to grow in the presence of 5-30 % (w/v) NaCl (optimum 20 %) and at 30-60 °C (optimum 45 °C). It grew at pH 5.5-7.8 and the optimum pH for growth was 6.8. The isolate required yeast extract for growth. Substrates utilized by strain CEJFT1B(T) as the sole carbon source included glucose, fructose, sucrose, pyruvate, Casamino acids and starch. Individual amino acids such as glutamate, lysine, methionine, serine, tyrosine, and amino acid mixtures formed by the Stickland reaction such as alanine-glycine, valine-proline, leucine-proline, isoleucine-proline were also utilized. Products of glucose fermentation were acetate (major product), butyrate, H2 and CO2. The genomic DNA G+C content of strain CEJFT1B(T) was 32.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CEJFT1B(T) should be assigned to the genus Sporohalobacter. The sequence similarity between strain CEJFT1B(T) and Sporohalobacter lortetii was 98.5 %, but DNA-DNA hybridization between the two strains revealed a relatedness value of 56.4 %, indicating that they are not related at the species level. The combination of phylogenetic analysis, DNA-DNA hybridization data, and differences in substrate utilization support the view that strain CEJFT1B(T) represents a novel species of the genus Sporohalobacter, for which the name Sporohalobacter salinus sp. nov. is proposed. The type strain is CEJFT1B(T) ( = DSM 26781(T) = JCM 19279(T)).


Asunto(s)
Bacterias Anaerobias Gramnegativas/clasificación , Lagos/microbiología , Filogenia , Salinidad , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Fermentación , Sedimentos Geológicos/microbiología , Bacterias Anaerobias Gramnegativas/genética , Bacterias Anaerobias Gramnegativas/aislamiento & purificación , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Túnez
6.
Genome Announc ; 1(1)2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-23469349

RESUMEN

Desulfovibrio hydrothermalis AM13T is a piezophilic, mesophilic, hydrogenotrophic sulfate-reducing bacterium collected from a deep-sea hydrothermal chimney on the East Pacific Rise (2,600 m depth, 13°N). We report the genome sequence of this bacterium, which includes a 3,702,934-bp chromosome and a circular plasmid of 5,328 bp.

7.
J Bacteriol ; 194(22): 6300-1, 2012 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23105050

RESUMEN

Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosinus meridiei, Desulfosporosinus orientis), and to grow under acidic conditions (Desulfosporosinus acidiphilus).


Asunto(s)
Genoma Bacteriano , Peptococcaceae/clasificación , Peptococcaceae/genética , ADN Bacteriano/genética , Datos de Secuencia Molecular , Especificidad de la Especie
8.
Extremophiles ; 14(3): 305-12, 2010 May.
Artículo en Inglés | MEDLINE | ID: mdl-20358236

RESUMEN

An obligately anaerobic, spore-forming, acidophilic sulfate-reducing bacterium, strain SJ4(T), was isolated from an acid mining effluent decantation pond sediment sample (pH around 3.0). Cells were Gram negative, non-motile, curved rods occurring singly. Strain SJ4(T) grew at pH 3.6-5.5 with an optimum at pH 5.2. Strain SJ4(T) utilized H(2), lactate, pyruvate, glycerol, glucose, and fructose as electron donors. Lactate and glucose were weakly used. Sulfate was used as electron acceptors, but not sulfite, elemental sulfur, arsenate (V), and fumarate. The G + C content of genomic DNA was 42.3 mol% (HPLC). 16S rRNA gene sequence analysis indicated that strain SJ4(T) belonged to the genus Desulfosporosinus within the family Peptococcaceae in the phylum Firmicutes. The level of 16S rRNA gene sequence similarity with other Desulfosporosinus species was 94.7-96.2%, D. orientis DSM 765(T) (similarity of 96.2%) and D. auripigmenti DSM 13351(T) (similarity of 95%) being its closest relatives. DNA-DNA relatedness values with D. orientis and D. auripigmenti were 16.5 and 31.8%, respectively. On the basis of phenotypic, phylogenetic, and genetic characteristics, strain SJ4(T) represents a novel species within the genus Desulfosporosinus, for which the name Desulfosporosinus acidiphilus sp. nov. is proposed. The type strain is SJ4(T) (=DSM 22704(T) = JCM 16185(T)).


Asunto(s)
Bacterias Gramnegativas/genética , Sulfatos/química , Bacterias Reductoras del Azufre/genética , Ácidos/química , Cromatografía Líquida de Alta Presión/métodos , Clasificación , ADN Bacteriano/química , Electrones , Bacterias Gramnegativas/clasificación , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Oxígeno/química , Fenotipo , Filogenia , ARN Ribosómico 16S/química , ARN Ribosómico 16S/genética , Bacterias Reductoras del Azufre/clasificación , Temperatura
9.
Extremophiles ; 11(1): 33-9, 2007 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-16932842

RESUMEN

A new alkaliphilic and moderately halophilic, strictly anaerobic, fermentative bacterium (strain IMP-300(T)) was isolated from a groundwater sample in the zone of the former soda lake Texcoco in Mexico. Strain IMP-300(T) was Gram-positive, non-sporulated, motile and rod-shaped. It grew within a pH range from 7.5 to 10.5, and an optimum at 9.5. The organism was obligately dependent on the presence of sodium salts. Growth showed an optimum at 35 degrees C with absence of growth above 45 degrees C. It fermented peptone and a few amino acids, preferentially arginine and ornithine, with production of acetate, propionate, and ammonium. Its fatty acid pattern was mainly composed of straight chain saturated, unsaturated, and cyclopropane fatty acids. The G + C content of genomic DNA was 40.0 mol%. Analysis of the 16S rRNA gene sequence indicated that the new isolate belongs to the genus Tindallia, in the low G + C Gram-positive phylum. Phylogenetically, strain IMP-300(T) has Tindallia californiensis, as closest relative with a 97.5% similarity level between their 16S rDNA gene sequences, but the DNA-DNA re-association value between the two DNAs was only 42.2%. On the basis of differences in genotypic, phenotypic, and phylogenetic characteristics, strain IMP-300(T) is proposed as a new species of the genus Tindallia, T. texcoconensis sp. nov. (type strain IMP-300(T ) = DSM 18041(T) = JCM 13990(T)).


Asunto(s)
Álcalis/metabolismo , Agua Dulce/microbiología , Bacilos Grampositivos Asporogénicos/clasificación , Bacilos Grampositivos Asporogénicos/aislamiento & purificación , Microbiología del Agua , Composición de Base , Carbonatos/metabolismo , ADN Bacteriano/análisis , Ácidos Grasos/metabolismo , Fermentación , Agua Dulce/química , Genotipo , Bacilos Grampositivos Asporogénicos/genética , Bacilos Grampositivos Asporogénicos/crecimiento & desarrollo , Bacilos Grampositivos Asporogénicos/metabolismo , Concentración de Iones de Hidrógeno , México , Fenotipo , Filogenia , ARN Ribosómico 16S/análisis , ARN Ribosómico 16S/genética , Ribotipificación , Cloruro de Sodio/metabolismo , Temperatura
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