Vaccination of squirrel monkeys (Saimiri spp.) with nanoparticle-based Toxoplasma gondii antigens: new hope for captive susceptible species.

Squirrel monkeys (Saimiri spp.), new world primates from South America, are very susceptible to toxoplasmosis. Numerous outbreaks of fatal toxoplasmosis in zoos have been identified around the world, resulting in acute respiratory distress and sudden death. To date, preventive hygiene measures or available treatments are not able to significantly reduce this mortality in zoos. Therefore, vaccination seems to be the best long-term solution to control acute toxoplasmosis. Recently, we developed a nasal vaccine composed of total extract of soluble proteins of Toxoplasma gondii associated with muco-adhesive maltodextrin-nanoparticles. The vaccine, which generated specific cellular immune responses, demonstrated efficacy against toxoplasmosis in murine and ovine experimental models. In collaboration with six French zoos, our vaccine was used as a last resort in 48 squirrel monkeys to prevent toxoplasmosis. The full protocol of vaccination includes two intranasal sprays followed by combined intranasal and s.c. administration. No local or systemic side-effects were observed irrespective of the route of administration. Blood samples were collected to study systemic humoral and cellular immune responses up to 1 year after the last vaccination. Vaccination induced a strong and lasting systemic cellular immune response mediated by specific IFN-γ secretion by peripheral blood mononuclear cells. Since the introduction of vaccination, no deaths of squirrel monkeys due to T. gondii has been observed for more than 4 years suggesting the promising usage of our vaccine. Moreover, to explain the high susceptibility of naive squirrel monkeys to toxoplasmosis, their innate immune sensors were investigated. It was observed that Toll-like and Nod-like receptors appear to be functional following T. gondii recognition suggesting that the extreme susceptibility to toxoplasmosis may not be linked to innate detection of the parasite.

Parturition in white rhinoceros.

In captive rhinoceros stillbirth and perinatal death are recorded at a rate of 6-17% in the various species. At the same time there is a substantial lack of knowledge on rhinoceros parturition. Yet, predicting parameters for birth and progress of parturition are fundamental for the recognition of dystocia and perinatal problems. Therefore, we here intended to pay close attention to the Achilles heel of the 1.5-2.5 year reproduction cycle in rhinoceros, the parturition. For the prediction of parturition we recorded timelines for pre-birth udder development, genital swelling, milk production, behavioral unrest, and decrease of serum progesterone concentration and the gestational length in 19 white rhinoceros. First, second and third labour stage, foetal presentation and events in perinatal period were recorded to describe normal parturition and establish a guideline for better birth management in rhinoceros. Udder development and genital swelling were observed 3 and 2 weeks prior birth, respectively. Milk production was observed to start up to 3 weeks prior birth and increased significantly in the last week with most significant increase one day before parturition to 50.6 ± 45.4 mL (p < 0.006). Serum progesterone concentration started to decrease 7 days prior parturition and more significantly 48 h before parturition. While behavioral unrest and first stage labour was not observed reliably in all females the break of foetal waters and thus the start of second stage labour was unmistakably observed. Second stage labour, when foetal membranes had ruptured until the foetus was born, took 1:50 ± 0:20 h:min. Eighty-four percent of fetuses were born in anterior presentation (n = 16/19) and the final expulsion took <25 min suggesting that this is the normal presentation in white rhinoceros. In the less frequent posterior presentation final expulsion took up to 47 min. Overall, 95% of calves were born alive. Calves were standing and nursing in 0:55 ± 0:12 min and 3:32 ± 0:53 h:min, respectively. In 10.5% of births (n = 2/19) in anterior presentation perinatal complications occurred. Stillbirth occurred once (5.3% n = 1/19) when the foetus was born in posterior presentation. The recorded gestational length was 506 ± 2d. Delivering live offspring is of key importance to establish a new generation and secure long-term survival of a species. Various pre-birth changes, significant decrease of serum progesterone 48 h prior birth, different labour stages, foetal presentation and perinatal events described here add substantial knowledge on the understanding of normal rhinoceros parturition and may help diagnose dystocia and perinatal complications.