Discovery of Small Molecules from Echinacea angustifolia Targeting RNA-Dependent RNA Polymerase of Japanese Encephalitis Virus.

The Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that causes viral encephalitis leading to neural damage, is a major threat in most Asian countries. The RNA-dependent RNA polymerase (RdRp) present in the viral genome is the key component for genome replication, making it an attractive target for antiviral drug development. In this study, the natural products from Echinacea angustifolia were retrieved for structure-based virtual screening against JEV-RdRp. The top six compounds (Echinacoside, Echinacin, Rutin, Cynaroside, Quercetagetin 7-glucoside, and Kaempferol-3-glucoside) were obtained based on the highest negative docking score, ADMET (absorption, distribution, metabolism, excretion, and toxicity), and molecular interaction. The computational analysis of these selected compounds against the co-crystallized ligands, i.e., ATP and GTP, were performed. Further, 100 ns molecular dynamic simulation and post-free binding energy calculation of all the selected compounds complexed with JEV-RdRP were performed to check the stability of the complexes. The obtained results showed considerable stability and intermolecular interaction with native ligand-binding site residues of JEV-RdRp. Hence, selected natural compounds are admissible inhibitors of JEV-RdRp protein and can be considered for future antiviral drug development studies.

Food safety and hygiene education improves the knowledge, attitudes, and practices of Saudi dietetics students.

The objective of the study is to evaluate the effect of food safety and hygiene course on the knowledge, attitudes, and practices of dietetics students. A repeated measure pre/post-intervention study was used to assess the knowledge, attitudes, and self-reported practices regarding food safety and hygiene among undergraduate dietetics students (n = 63) enrolled in a course on food safety and hygiene in Saudi Arabia. Students were asked to complete an online questionnaire divided into five sections that covered key food-safety concepts before and at the end of the course to determine changes in these variables. Overall, students' knowledge, attitudes, and practices improved significantly after attending the course on food safety and hygiene. Scores for total knowledge increased from 16.51±2.60 before the course to 20.60±2.01 after it (p < 0.001). The total score for attitudes improved from 9.16±1.43 before the course to 9.92±0.27 after it (p < 0.001). The total score for practices increased from 11.0±3.10 before the course to 14.78±3.41 after it (p < 0.001).The course on food safety and hygiene helped to improve the knowledge, attitudes, and self-reported practices of food safety by dietetics students.

Managing Gut Microbiota through In Ovo Nutrition Influences Early-Life Programming in Broiler Chickens.

The chicken gut is the habitat to trillions of microorganisms that affect physiological functions and immune status through metabolic activities and host interaction. Gut microbiota research previously focused on inflammation; however, it is now clear that these microbial communities play an essential role in maintaining normal homeostatic conditions by regulating the immune system. In addition, the microbiota helps reduce and prevent pathogen colonization of the gut via the mechanism of competitive exclusion and the synthesis of bactericidal molecules. Under commercial conditions, newly hatched chicks have access to feed after 36-72 h of hatching due to the hatch window and routine hatchery practices. This delay adversely affects the potential inoculation of the healthy microbiota and impairs the development and maturation of muscle, the immune system, and the gastrointestinal tract (GIT). Modulating the gut microbiota has been proposed as a potential strategy for improving host health and productivity and avoiding undesirable effects on gut health and the immune system. Using early-life programming via in ovo stimulation with probiotics and prebiotics, it may be possible to avoid selected metabolic disorders, poor immunity, and pathogen resistance, which the broiler industry now faces due to commercial hatching and selection pressures imposed by an increasingly demanding market.

Responses of consumers with food allergy to the new allergen-labelling legislation in Saudi Arabia: a preliminary survey.

OBJECTIVE: Preventing a food allergy reaction depends primarily on eliminating allergens from the diet. In October 2019, the Saudi Food and Drug Authority (SFDA) introduced new legislation requiring food establishments providing and selling non-prepacked foods to state the presence of the top fourteen food allergens on their menus. The current study aimed to assess the allergen-labelling knowledge, practices, preferences and perceptions towards the new SFDA allergen-labelling legislation among consumers with food allergy in Saudi Arabia. DESIGN: Observational cross-sectional study using an online questionnaire. SETTING: Saudi Arabia; February - March 2020. PARTICIPANTS: Residents of Saudi Arabia with food allergy (n 427), aged 18-70 years. RESULTS: Among participants, only 28·1 % knew that there were governmental regulations in Saudi Arabia regarding food-allergen labelling and approximately two-thirds (67 %) check labels on prepacked food products for allergens. The majority of the participants preferred food products carrying safety statements (84·1 %) and symbols (80·1 %). A total of 47·1 % were aware that regulations in Saudi Arabia require allergens to be declared in ingredient lists, while 51·3 % were aware that advisory allergen labelling is not required by law. Only 26·2 % were aware of the new SFDA legislation regarding provision of allergen information by food establishments. However, the majority (94·4 %) were supportive of the new legislation, and most of them were more likely to eat at restaurants that reported allergen information for food items on the menu. CONCLUSIONS: The new SFDA food allergen-labelling legislation needs to be more widely and effectively disseminated to increase the level of awareness among adults with food allergy in Saudi Arabia.