Acute myeloid leukemia due to germline CEBPA mutation in a Syrian family.

BACKGROUND: Familial cases of adult acute myeloid leukemia (AML) with germline-mutated CCAAT/enhancer-binding protein-α (CEBPA) gene are a rare entity classified in World Health Organization (WHO) classification 2016. Most families reported in the literature show an autosomal dominant inheritance pattern consistent with a single-gene mutation. METHODS: Here we studied a Syrian family with four individuals suffering from AML for CEBPA gene mutations by Sanger sequencing. RESULTS: The father, his three affected, and one yet unaffected child had the same mutation in the N-terminal region of CEBPA (c.198dupC), resulting in termination at Tyr67Leufs*41. All affected family members had a good primary response to chemotherapy and achieved complete remission. CONCLUSION: Overall, another AML family with CEBPA gene mutation is added to the literature, presenting with yet unreported FAB subtype M5 and absence of CD7 expression in some family members.

Decontamination of ubiquitous harmful microbial lineages in water using an innovative Zn2Ti0.8Fe0.2O4 nanostructure: dielectric and terahertz properties.

Many ubiquitous dangerous microbial lines could originate in different sources of polluted water and be distributed to tap water, which could cause multiple types of illnesses to humans and livestock. Despite enormous attempts to guarantee safety of potable water, these species are still regarded to be threated prevalent health issues and concerns. However, these species need a powerful disinfectant to be removed from contaminated water for receiving clean and healthy water. This study was therefore conducted to produce magnificent magnetic iron titanate zinc nano-particles (Zn2Ti0.8Fe0.2O4 MNPs) as a sophisticated approach for drinking water (DW) and wastewater purification. The identification of crystalline phase, dielectric and terahertz spectroscopy of iron zinc titanate nanostructure prepared via acidic sol-gel process and calcined at 800 °C. Results show that the formation of cubic structure for Zn2TiO4 phase, and the dielectric constant (ε') decreased with the higher frequency, tan (δ) has higher values at lower frequency and the conductivity increases relatively with frequency that attributes to the high resistive grain boundaries. Absorption coefficient, refractive index and dielectric properties of iron zinc titanate nano-particles was estimated via time domain-terahertz spectrometer and adjusted via the applied electric field. In particular, the Gram-negative bacteria were more prone than other microbes tested to the Magnetic Nano-Particles (MNPs). Results also was ascertained that the minimum inhibitory concentration (MIC) was 25 ppm at 30 min for E. coli and Salmonella enterica, 45 min for Listeria monocyteogens, Staphylococcus aureus, and Candida albicans and 60 min for Aspergillus niger with a noticeable bactericidal impact. Results exhibit that the MNPs explored are non-toxic and protected for individuals and the environment. MNPs can, therefore, be proposed as an expedient and impressive nano-scale applicant for inactivation during the drinking water and wastewater conservation of the prevailing dangerous microbes.

Green sol-gel synthesis of novel nanoporous copper aluminosilicate for the eradication of pathogenic microbes in drinking water and wastewater treatment.

We used a green sol-gel synthesis method to fabricate a novel nanoporous copper aluminosilicate (CAS) material. Nanoporous CAS was characterized using X-ray powder diffraction (XRD), field emission transmission and scanning electron microscopies (FE-TEM/FE-SEM), Fourier transform infrared (FTIR) spectroscopy, and optical analyses. The CAS was also evaluated for use as a promising disinfectant for the inactivation of waterborne pathogens. The antimicrobial action and minimum inhibitory concentration (MIC) of this CAS disinfectant were determined against eight microorganisms (Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Candida albicans, and Aspergillus niger). An antimicrobial susceptibility testing of CAS was measured. Results of disc diffusion method pointed out that the diameters of the zone using well diffusion were wider than disc diffusion methods, and the findings also showed that the MIC of the CAS disinfectant against E. coli, S. enterica, and P. aeruginosa was 100 mg/L within 20 min of contact time. Meanwhile, the MIC of the CAS disinfectant was 100 mg/L within 40 min of contact time for the other strains. The efficacy of antimicrobial action (100%) reached within 20 to 40 min against all tested microbes. Herein, the antimicrobial susceptibility testing of CAS disinfectant showed no toxicity for human and bacterial cells. It can be concluded that nanoporous CAS is a promising, economically, and worthy weapon for water disinfection.

Dorzolamide synergizes the antitumor activity of mitomycin C against Ehrlich's carcinoma grown in mice: role of thioredoxin-interacting protein.

The antitumor activity of carbonic anhydrase (CA) inhibitors is attributed to their ability to induce a state of intracellular acidification. In fact, acidic intracellular pH was demonstrated to upregulate several tumor suppressor proteins and increase the activity of many chemotherapies. The present study aimed to investigate the antitumor activity of the CA inhibitor, dorzolamide, in combination with mitomycin C and to study the effect of these drugs on tumoral thioredoxin-interacting protein (TXNIP) as well as tumor cell proliferation and apoptosis. Solid tumors were induced by subcutaneous inoculation of Ehrlich's ascites carcinoma (EAC) cells in female mice. Mice were treated with dorzolamide (3, 10, or 30 mg/kg/day, i.p.) and/or mitomycin C (1 mg/kg, i.p.) weekly for 3 weeks. Treatment with mitomycin C increased TXNIP level in EAC solid tumors in mice. Likewise, treatment with dorzolamide upregulated TXNIP and p53 while downregulated bcl-2. Both drug therapies increased tumoral caspase 9, caspase 3, and PARP-1 cleavage in addition to decreasing the proliferative Ki-67-stained nuclear fraction. Indeed, a synergistic effect was detected between mitomycin C and dorzolamide. The current data demonstrated that the antitumor activity of mitomycin C and dorzolamide was, at least in part, mediated through stimulating tumoral expression of TXNIP and enhancing tumor apoptosis.