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1.
Open Forum Infect Dis ; 10(1): ofac682, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36655187

RESUMEN

Background: Acanthamoeba is a free-living ameba that can cause severe disease affecting the central nervous system, skin, sinuses, and other organs, particularly in immunocompromised individuals. These rare but severe infections are often fatal, yet incompletely described. Methods: Cases included were either reported to the Centers for Disease Control and Prevention (CDC) Free-Living Ameba program or published in scientific literature. Characteristics of all patients in the United States with laboratory-confirmed non-keratitis Acanthamoeba infections were described using descriptive statistics, and associations with survival were determined using χ2 and Fisher exact tests. Results: Of 173 patients identified, 71% were male and the median age was 44 years (range, 0-87 years). Of these, 26 (15%) survived. Most patients (88%) had at least 1 immunocompromising condition, most commonly human immunodeficiency virus (39%), cancer (28%), and solid organ or hematopoietic stem cell transplant (28%). Granulomatous amebic encephalitis (GAE) was the most common disease presentation (71%). Skin (46%), sinuses (29%), lungs (13%), and bone (6%) were also involved. Nearly half of patients (47%) had involvement of >1 organ system. Survival was less frequent among those with GAE (3%, P < .001) compared with cutaneous disease, rhinosinusitis, or multiorgan disease not including GAE. Of 7 who received the currently recommended treatment regimen, 5 (71%) survived. Conclusions: Non-keratitis Acanthamoeba infections occur primarily in immunocompromised individuals and are usually fatal. Survival may be associated with disease presentation and treatment. Providers who care for at-risk patients should be aware of the various disease manifestations to improve early recognition and treatment.

2.
J Zoo Wildl Med ; 52(1): 310-314, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33827191

RESUMEN

Granulomatous amoebic encephalitis caused by the free-living amoeba Balamuthia mandrillaris is a highly fatal disease that was first isolated from a mandrill (Mandrillus sphinx), and has since been diagnosed in several nonhuman primates including orangutans. Indirect immunofluorescence antibody (IFA) techniques for Balamuthia have been used in the fields of human medicine and epidemiology both for exposure assessment and screening of clinical patients for antemortem diagnosis. Stored serum samples from five captive Northwest Bornean orangutans (Pongo pygmaeus pygmaeus), including one who had died from B. mandrillaris infection, housed at a single facility were screened with a human IFA assay for B. mandrillaris. Only the single, clinically affected individual was seropositive, and the results suggest that the use of the available human B. mandrillaris IFA assay is a novel diagnostic option for detection of Balamuthia antibodies in this species. A validated screening serological test could be used in individuals exhibiting signs consistent with granulomatous amoebic encephalitis to facilitate earlier antemortem diagnosis of Balamuthia infection, which is critical if treatment is to be pursued. This pilot study presents the use of serological detection methods for B. mandrillaris screening in a nonhuman primate. Subsequent use of the B. mandrillaris IFA assay in the larger captive population should be pursued for validation of the test and to provide further information on seroprevalence and evaluation of risk factors for exposure to Balamuthia and subsequent development of disease.


Asunto(s)
Amebiasis/veterinaria , Enfermedades del Simio Antropoideo/diagnóstico , Balamuthia mandrillaris , Técnica del Anticuerpo Fluorescente Indirecta/métodos , Pongo pygmaeus/parasitología , Amebiasis/diagnóstico , Animales , Animales de Zoológico , Enfermedades del Simio Antropoideo/parasitología , Femenino , Humanos
4.
5.
Parasitol Res ; 119(1): 339-344, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31734864

RESUMEN

We present the first recognized case of primary amebic meningoencephalitis (PAM) caused by Naegleria fowleri in a 15-year-old male from Bangladesh. He performed daily nasal rinsing with untreated ground water and bathed in untreated ground water or river water, which likely exposed him to N. fowleri.


Asunto(s)
Infecciones Protozoarias del Sistema Nervioso Central/parasitología , Naegleria fowleri/aislamiento & purificación , Adolescente , Animales , Bangladesh , Resultado Fatal , Agua Dulce/parasitología , Humanos , Masculino
6.
J Am Vet Med Assoc ; 255(2): 219-223, 2019 07 15.
Artículo en Inglés | MEDLINE | ID: mdl-31260399

RESUMEN

CASE DESCRIPTION A 20-year-old female south-central black rhinoceros (Diceros bicornis minor) was evaluated because of an acute onset of CNS deficits. CLINICAL FINDINGS The rhinoceros had no history of illness. Clinical signs included acute lethargy, ataxia, and decreased appetite. Hematologic abnormalities included leukocytosis with neutrophilia and a profound left shift. Results of serum biochemical analysis revealed hypophosphatemia but no other abnormalities. Results of a quantitative PCR assay for West Nile virus and an assay for anti-Neosporum caninum antibodies in serum were negative; the patient was seropositive for multiple Leptospira serovars. TREATMENT AND OUTCOME Antimicrobials and anti-inflammatory agents were administered, but the condition of the rhinoceros worsened overnight; despite treatment with additional anti-inflammatory and antimicrobial agents, IV fluids, and thiamine, it became obtunded and died of respiratory arrest ≤ 24 hours later. Necropsy revealed severe, diffuse, suppurative, and histiocytic meningo-encephalomyelitis involving the cerebrum, cerebellum, and spinal cord. Amebic trophozoites were observed on histologic examination of affected tissue. Infection with Naegleria fowleri was confirmed by results of immuno-histochemical analysis and a multiplex real-time PCR assay. CLINICAL RELEVANCE Findings suggested that south-central black rhinoceros are susceptible to the free-living ameba N fowleri. Ameba-induced meningoencephalomyelitis should be considered as a differential diagnosis for rhinoceros that have an acute onset of neurologic signs. Diagnosis of N fowleri infection in an animal has a profound public health impact because of potential human exposure from the environment and the high fatality rate in people with N fowleri infection.


Asunto(s)
Amoeba , Encefalomielitis/veterinaria , Naegleria fowleri , Animales , Femenino , Humanos , Perisodáctilos
7.
Clin Infect Dis ; 68(11): 1815-1822, 2019 05 17.
Artículo en Inglés | MEDLINE | ID: mdl-30239654

RESUMEN

BACKGROUND: Balamuthia mandrillaris is a free-living ameba that causes rare, nearly always fatal disease in humans and animals worldwide. B. mandrillaris has been isolated from soil, dust, and water. Initial entry of Balamuthia into the body is likely via the skin or lungs. To date, only individual case reports and small case series have been published. METHODS: The Centers for Disease Control and Prevention (CDC) maintains a free-living ameba (FLA) registry and laboratory. To be entered into the registry, a Balamuthia case must be laboratory-confirmed. Several sources were used to complete entries in the registry, including case report forms, CDC laboratory results, published case reports, and media information. SAS© version 9.3 software was used to calculate descriptive statistics and frequencies. RESULTS: We identified 109 case reports of Balamuthia disease between 1974 and 2016. Most (99%) had encephalitis. The median age was 36 years (range 4 months to 91 years). Males accounted for 68% of the case patients. California had the highest number of case reports, followed by Texas and Arizona. Hispanics constituted 55% for those with documented ethnicity. Exposure to soil was commonly reported. Among those with a known outcome, 90% of patients died. CONCLUSIONS: Balamuthia disease in the United States is characterized by a highly fatal encephalitis that affects patients of all ages. Hispanics were disproportionately affected. The southwest region of the United States reported the most cases. Clinician awareness of Balamuthia as a cause of encephalitis might lead to earlier diagnosis and initiation of treatment, resulting in better outcomes.


Asunto(s)
Amebiasis/epidemiología , Balamuthia mandrillaris/patogenicidad , Infecciones Protozoarias del Sistema Nervioso Central/epidemiología , Encefalitis Infecciosa/epidemiología , Sistema de Registros , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Amebiasis/mortalidad , Amebiasis/fisiopatología , Infecciones Protozoarias del Sistema Nervioso Central/mortalidad , Infecciones Protozoarias del Sistema Nervioso Central/fisiopatología , Niño , Preescolar , Femenino , Humanos , Lactante , Encefalitis Infecciosa/mortalidad , Encefalitis Infecciosa/fisiopatología , Masculino , Persona de Mediana Edad , Análisis de Secuencia de ADN , Estados Unidos/epidemiología , Adulto Joven
8.
Am J Trop Med Hyg ; 100(2): 311-313, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30526733

RESUMEN

Amoebic trophozoites were identified in the cervicovaginal smear of a U.S. patient without travel history at the time of intrauterine device (IUD) removal. Subsequent morphologic analysis and DNA sequencing identified a mixed cervicovaginal colonization of the female genital tract with both Entamoeba gingivalis and Entamoeba polecki in association with Actinomyces species bacteria. This highlights to the potential for colonization of the genital tract with E. gingivalis, particularly in association with IUD placement, and represents the first report of E. polecki in this context.


Asunto(s)
Actinomyces/genética , Actinomicosis/diagnóstico , Entamoeba/genética , Entamebiasis/diagnóstico , Dispositivos Intrauterinos , Actinomyces/clasificación , Actinomyces/aislamiento & purificación , Actinomicosis/parasitología , Cuello del Útero/microbiología , Cuello del Útero/parasitología , Coinfección , Entamoeba/clasificación , Entamoeba/aislamiento & purificación , Entamebiasis/parasitología , Femenino , Humanos , Dispositivos Intrauterinos/microbiología , Dispositivos Intrauterinos/parasitología , Prueba de Papanicolaou , Vagina/microbiología , Vagina/parasitología , Adulto Joven
9.
Pediatr Transplant ; 21(8)2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28921764

RESUMEN

Acanthamoeba encephalitis is a rare, often fatal condition, particularly after HSCT, with 9 reported cases to date in the world literature. Our case was originally diagnosed with ALL at age 3 years, and after several relapses underwent HSCT at age 9 years. At 17 years of age, he was diagnosed with secondary AML for which he underwent a second allogeneic HSCT. He presented with acute-onset worsening neurological deficits on day +226 after the second transplant and a post-mortem diagnosis of Acanthamoeba encephalitis was established, with the aid of the CDC.


Asunto(s)
Acanthamoeba/aislamiento & purificación , Amebiasis/diagnóstico , Trasplante de Células Madre Hematopoyéticas , Huésped Inmunocomprometido , Encefalitis Infecciosa/diagnóstico , Leucemia Mieloide Aguda/terapia , Adolescente , Amebiasis/inmunología , Resultado Fatal , Humanos , Encefalitis Infecciosa/inmunología , Leucemia Mieloide Aguda/inmunología , Masculino
10.
Curr Infect Dis Rep ; 18(10): 31, 2016 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-27614893

RESUMEN

Primary amebic meningoencephalitis (PAM) is a devastating infection of the brain caused by the thermophilic free-living ameba, Naegleria fowleri. Infection can occur when water containing the ameba enters the body through the nose, usually during recreational water activities such as swimming or diving. Historically, in the USA, cases were mostly reported from the warmer southern-tier states. In the last 5 years, several notable changes have been documented in PAM epidemiology including a northward expansion of infections and new types of water exposures. The recent reports of two PAM survivors provide hope for improved outcomes with early diagnosis and aggressive treatment. Advanced molecular laboratory tools such as genome sequencing might provide more insight into the pathogenicity of N. fowleri. Clinicians treating patients with meningitis and warm freshwater exposure are encouraged to consider PAM in their differential diagnoses.

11.
PLoS Negl Trop Dis ; 10(5): e0004697, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-27152855

RESUMEN

Entamoeba histolytica causes intestinal disease in endemic settings throughout the world. Diagnosis of E. histolytica infection would be improved by the identification of biomarkers that are expressed by cysts of E. histolytica, but not by cysts of closely related commensal species of Entamoeba. Herein, we describe two novel monoclonal antibodies (1A4 and 1D3) produced against a spacer region of the E. histolytica Jacob2 lectin, an outer cyst wall protein. These reagents demonstrated no cross-reaction to E. dispar recombinant antigen and low picomolar molecular detection limits when paired in ELISA sandwich assays. In an immunofluorescence microscopy assay, the α-Jacob2 murine antibodies labeled cysts of three xenically cultured E. histolytica isolates but did not label cysts of three E. bangladeshi isolates. Monoclonal antibody 1A4 did not cross-react with xenic cultures of three E. dispar isolates, demonstrating specificity to E. histolytica, while monoclonal antibody 1D3 cross-reacted with two out of three E. dispar isolates. Both antibodies labeled cysts in formalin-fixed slides, a potential logistical advantage in some settings. The monoclonal antibody 1A4 was also used in an immunofluorescence microscopy assay with formalin-fixed stool specimens. Seven out of ten ELISA-positive stool specimens exhibited 1A4-labeled cyst-like objects, compared to one out of seven ELISA-negative specimens. These results demonstrate that antibodies generated against the flexible spacer of E. histolytica Jacob2 lectin recognize and bind to Jacob2 protein in whole cysts and are capable of differentiating Entamoeba species in fixed specimens. Thus, Jacob2 is a promising biomarker for use in diagnosing E. histolytica infection.


Asunto(s)
Anticuerpos Monoclonales/inmunología , Anticuerpos Antiprotozoarios/inmunología , Entamoeba histolytica/inmunología , Ensayo de Inmunoadsorción Enzimática/métodos , Proteínas Protozoarias/inmunología , Adolescente , Secuencia de Aminoácidos , Animales , Biomarcadores , Niño , Preescolar , Clonación Molecular , Humanos , Lactante , Ratones , Enquistamiento de Parásito/fisiología , Sensibilidad y Especificidad , Especificidad de la Especie
12.
Ann Neurol ; 78(5): 722-30, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-26290222

RESUMEN

OBJECTIVE: Identification of a particular cause of meningoencephalitis can be challenging owing to the myriad bacteria, viruses, fungi, and parasites that can produce overlapping clinical phenotypes, frequently delaying diagnosis and therapy. Metagenomic deep sequencing (MDS) approaches to infectious disease diagnostics are known for their ability to identify unusual or novel viruses and thus are well suited for investigating possible etiologies of meningoencephalitis. METHODS: We present the case of a 74-year-old woman with endophthalmitis followed by meningoencephalitis. MDS of her cerebrospinal fluid (CSF) was performed to identify an infectious agent. RESULTS: Sequences aligning to Balamuthia mandrillaris ribosomal RNA genes were identified in the CSF by MDS. Polymerase chain reaction subsequently confirmed the presence of B. mandrillaris in CSF, brain tissue, and vitreous fluid from the patient's infected eye. B. mandrillaris serology and immunohistochemistry for free-living amoebas on the brain biopsy tissue were positive. INTERPRETATION: The diagnosis was made using MDS after the patient had been hospitalized for several weeks and subjected to costly and invasive testing. MDS is a powerful diagnostic tool with the potential for rapid and unbiased pathogen identification leading to early therapeutic targeting.


Asunto(s)
Amebiasis/diagnóstico , Amebiasis/genética , Balamuthia mandrillaris/genética , Meningoencefalitis/diagnóstico , Meningoencefalitis/genética , Análisis de Secuencia de ARN/métodos , Anciano , Amebiasis/líquido cefalorraquídeo , Animales , Encéfalo/microbiología , ADN Protozoario/genética , Femenino , Genómica , Humanos , Meningoencefalitis/líquido cefalorraquídeo , Reacción en Cadena de la Polimerasa , Cuerpo Vítreo/microbiología
13.
PLoS One ; 7(2): e32042, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22363793

RESUMEN

The generation of affinity reagents, usually monoclonal antibodies, remains a critical bottleneck in biomedical research and diagnostic test development. Recombinant antibody-like proteins such as scFv have yet to replace traditional monoclonal antibodies in antigen detection applications, in large part because of poor performance of scFv in solution. To address this limitation, we have developed assays that use whole yeast cells expressing scFv on their surfaces (yeast-scFv) in place of soluble purified scFv or traditional monoclonal antibodies. In this study, a nonimmune library of human scFv displayed on the surfaces of yeast cells was screened for clones that bind to recombinant cyst proteins of Entamoeba histolytica, an enteric pathogen of humans. Selected yeast-scFv clones were stabilized by lyophilization and used in detection assay formats in which the yeast-scFv served as solid support-bound monoclonal antibodies. Specific binding of antigen to the yeast-scFv was detected by staining with rabbit polyclonal antibodies. In flow cytometry-based assays, lyophilized yeast-scFv reagents retained full binding activity and specificity for their cognate antigens after 4 weeks of storage at room temperature in the absence of desiccants or stabilizers. Because flow cytometry is not available to all potential assay users, an immunofluorescence assay was also developed that detects antigen with similar sensitivity and specificity. Antigen-specific whole-cell yeast-scFv reagents can be selected from nonimmune libraries in 2-3 weeks, produced in vast quantities, and packaged in lyophilized form for extended shelf life. Lyophilized yeast-scFv show promise as low cost, renewable alternatives to monoclonal antibodies for diagnosis and research.


Asunto(s)
Anticuerpos Monoclonales/economía , Antígenos de Protozoos/inmunología , Entamoeba histolytica/inmunología , Liofilización/economía , Liofilización/métodos , Saccharomyces cerevisiae/metabolismo , Anticuerpos de Cadena Única/inmunología , Anticuerpos Monoclonales/inmunología , Antígenos de Protozoos/economía , Costos y Análisis de Costo , Técnica del Anticuerpo Fluorescente , Humanos , Indicadores y Reactivos/economía , Límite de Detección , Microscopía Fluorescente , Sondas Moleculares/economía , Sondas Moleculares/metabolismo , Proteínas Protozoarias/inmunología , Saccharomyces cerevisiae/citología , Anticuerpos de Cadena Única/economía , Factores de Tiempo
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