Exogenous ascorbic acid as a potent regulator of antioxidants, osmo-protectants, and lipid peroxidation in pea under salt stress.

Pea (Pisum sativum L.), a globally cultivated leguminous crop valued for its nutritional and economic significance, faces a critical challenge of soil salinity, which significantly hampers crop growth and production worldwide. A pot experiment was carried out in the Botanical Garden, The Islamia University of Bahawalpur to alleviate the negative impacts of sodium chloride (NaCl) on pea through foliar application of ascorbic acid (AsA). Two pea varieties Meteor (V1) and Sarsabz (V2) were tested against salinity, i.e. 0 mM NaCl (Control) and 100 mM NaCl. Three levels of ascorbic acid 0 (Control), 5 and 10 mM were applied through foliar spray. The experimental design was completely randomized (CRD) with three replicates. Salt stress resulted in the suppression of growth, photosynthetic activity, and yield attributes in pea plants. However, the application of AsA treatments effectively alleviated these inhibitory effects. Under stress conditions, the application of AsA treatment led to a substantial increase in chlorophyll a (41.1%), chl. b (56.1%), total chl. contents (44.6%) and carotenoids (58.4%). Under salt stress, there was an increase in Na+ accumulation, lipid peroxidation, and the generation of reactive oxygen species (ROS). However, the application of AsA increased the contents of proline (26.9%), endogenous AsA (23.1%), total soluble sugars (17.1%), total phenolics (29.7%), and enzymatic antioxidants i.e. SOD (22.3%), POD (34.1%) and CAT (39%) in both varieties under stress. Salinity reduced the yield attributes while foliarly applied AsA increased the pod length (38.7%), number of pods per plant (40%) and 100 seed weight (45.2%). To sum up, the application of AsA alleviated salt-induced damage in pea plants by enhancing photosynthetic pigments, both enzymatic and non-enzymatic activities, maintaining ion homeostasis, and reducing excessive ROS accumulation through the limitation of lipid peroxidation. Overall, V2 (Sarsabz) performed better as compared to the V1 (Meteor).

Enhancing Sugarcane Yield and Sugar Quality through Optimal Application of Polymer-Coated Single Super Phosphate and Irrigation Management.

The judicious use of crop input is of prime importance for achieving a considerable output with a low-cost input. A two-year field experimentation was executed to assess the effect of varying polymer-coated single super phosphate (SSP) regimes on the yield and quality of sugarcane under differential water regimes. A two-factor study was executed under a randomized complete block design with a split-plot arrangement. The CPF-249 sugarcane variety was planted during the 2019-2020 period and the 2020-2021 period. The experiment consisted of four levels of polymer-coated SSP, i.e., control, 90, 110, and 130 kg ha-1, and three water regimes, which consisted of a number of irrigations, i.e., 18 irrigations, 15 irrigations, and 12 irrigations. Moreover, the water regimes were kept in the main plot, whereas the polymer-coated supplement was allocated in a subplot and replicated thrice. The data on the yield components and sugar-related traits were recorded during both years of study, and the treatment means were differentiated using an LSD test at a 95% confidence interval. Summating the findings of this study, a significant variation was revealed under the subject levels of both factors. Statistically, a 110 kg ha-1 polymer-coated SSP dose, along with 18 irrigations, declared the highest millable canes, stripped cane yield, and unstripped cane yield, followed by the 130 kg ha-1 treatment. Additionally, the highest pol% and cane sugar recovery % were recorded under 12 irrigations along with 130 kg ha-1 during both years. Similarly, the °Brix value was also significantly affected by 12 irrigations when 110 kg ha-1 of polymer-coated SSP was used. The unstripped cane yield had a strong positive correlation with the stripped cane yield, millable canes, and the number of internodes. Moreover, the commercial cane sugar % resulted in a strong positive correlation with the pol%, whereas the cane sugar recovery % revealed a strong positive correlation with the pol% and commercial cane sugar %.

Optimizing Sugarcane Growth, Yield, and Quality in Different Ecological Zones and Irrigation Sources Amidst Environmental Stressors.

The imbalanced use of fertilizers and irrigation water, particularly supplied from groundwater, has adversely affected crop yield and harvest quality in sugarcane (Saccharum officinarum L.). In this experiment, we evaluated the impact of potassium (K) and micronutrients [viz. Zinc (Zn), Iron (Fe), and Boron (B)] application and irrigation water from two sources, viz. canal, and tube well water on sugarcane growth, yield, and cane quality under field trails. Water samples from Mardan (canal water) and Rahim Yar Khan (tube well water) were analyzed for chemical and nutritional attributes. The results revealed that tube well water's electrical conductivity (EC) was three-fold that of canal water. Based on the EC and total dissolved salts (TDS), 83.33% of the samples were suitable for irrigation, while the sodium adsorption ratio (SAR) indicated only a 4.76% fit and a 35.71% marginal fit compared with canal water. Furthermore, the application of K along with B, Fe, and Zn had led to a significant increase in cane height (12.8%, 9.8%, and 10.6%), cane girth (15.8%, 15.6%, and 11.6%), cane yield (13.7%, 12.3%, and 11.5%), brix contents (14%, 12.2%, and 13%), polarity (15.4%, 1.4%, and 14%), and sugar recovery (7.3%, 5.9%, and 6%) in the tube well irrigation system. For the canal water system, B, Fe, and Zn increased cane height by 15.3%, 13.42%, and 11.6%, cane girth by 13.9%, 9.9%, and 6.5%, cane yield by 42.9%, 43.5%, and 42%, brix content by 10.9%, 7.7%, and 8%, polarity by 33.4%, 28%, and 30%, and sugar recovery by 4.0%, 3.9%, and 2.0%, respectively, compared with sole NPK application. In conclusion, the utilization of tube well water in combination with canal water has shown better results in terms of yield and quality compared with the sole application of canal water. In addition, the combined application of K and B significantly improved sugarcane yields compared with Zn and Fe, even with marginally suitable irrigation water.

Comparative Analysis of Growth and Physiological Responses of Sugarcane Elite Genotypes to Water Stress and Sandy Loam Soils.

Stumpy irrigation water availability is extremely important for sugarcane production in Pakistan today. This issue is rising inversely to river flow due to inadequate water distribution and an uneven rainfall pattern. Sugarcane growth faces a shortage of available water for plant uptake due to the low water-holding capacity of sandy loam soil, particularly under conventional flood irrigation methods. To address this problem, sugarcane clones were evaluated for their agronomic and physiological traits under conditions of low water availability in sandy loam soil. Ten cane genotypes, HSF-240, SPF-213, CPF-249, CP 77-400, S2008-FD-19, S2006-US-469, S2007-AUS-384, S2003-US-633, S2003-US-127, and S2006-US-658, were exposed to four levels of water deficit created through skip irrigations. These deficit levels occurred during the 9th, 11th, 13th, and 16th irrigations at alternate deficit levels between 2020 and 2022. Physiological data were collected during the tillering and grand growth stages (elongation) in response to the water deficit. The sugarcane clones S2006-US-658, S2007-AUS-384, and HSF-240 exhibited resistance to low water availability at both the tillering and grand growth stages. Following them, genotypes S2006-US-658, S2007-AUS-384, and HSF-240 performed better and were also found to be statistically significant. Clones susceptible to water deficit in terms of growth and development were identified as CP 77-400, S2008-FD-19, S2006-US-469, and S2003-US-633. These genotypes showed reduced photosynthetic rate, transpiration rate, stomatal conductance, relative water content, cane yield, and proline content under stressed conditions. Therefore, genotypes S2006-US-658, S2007-AUS-384, and HSF-240 were better performers concerning physiological traits under water deficit and sandy loam soil in both years. Moreover, a significant positive correlation was assessed between agronomic traits and photosynthetic rats. This study highlights that sugarcane can sustain its growth and development even with less irrigation frequency or moisture availability, albeit with certain specific variations.

Phytochemical screening and allelopathic potential of phytoextracts of three invasive grass species.

Undoubtedly, it is important to remain vigilant and manage invasive grasses to prevent their spread and mitigate their negative impact on the environment. However, these aggressive plants can also play a beneficial role in certain contexts. For example, several invasive grasses provide valuable forage for livestock and have disease control potential. Therefore, a research experiment was conducted to explore the pros and cons of this approach, not only for surrounding vegetation but also for human and animal disease control. The study is primarily focused on developing livestock feed, plant-derived herbicides, and an understanding of the phytotoxic effects of invasive species. All plant parts of Cenchrus ciliaris L., Polypogon monspeliansis L., and Dicanthium annulatum (Forssk.) Stapf, were tested for their phyto-chemical screening, proximate, and toxicity analysis which was caused by the methanolic extract of these grass species. Qualitative phytochemical screening tests were performed for proximate composition analysis and toxicity assessment essays. The phytochemical analysis revealed the positive results for alkaloids, flavonoids, coumarins, phenols, saponins, and glycosides, while negative for tannins. Comparison of proximate analysis intimated maximum moisture (10.8%) and crude fat (4.1%) in P. monspeliensis, whereas maximum dry matter (84.1%), crude protein (13.95%), crude fiber (11%), and ash (7.2%) in D. annulatum. Five (10, 100, 500, 100, 10,000 ppm) and three (10, 1000, 10,000 ppm) different concentrations of methanolic extract prepared from C. ciliaris, P. monspeliansis, and D. annulatum were used respectively for root inhibition and seed germination essay. Furthermore, three different concentrations (10, 30, 50 mg) of plant fine powder were used for sandwich method test. There was a significant decline in the growth rate of experimental model radish seeds (P > 0.005), and results from sandwich method tests showed suppressed growth of root hairs, inhibiting the anchoring of the radish seed. In comparison, results manifest that; P. monspeliansis indicated an upsurge of inhibition (66.58% at 10,000 ppm), D. annulatum revealed soar germination (75.86% in controlled conditions), and C. ciliaris exhibited dramatic shoot up of inhibition because of sandwich method test (14.02% at 50 mg). In conclusion, although grasses are toxic, it is important to consider the beneficiary account.

Mitigating Ammonia and Greenhouse Gaseous Emission From Arable Land by Co-application of Zeolite and Biochar.

The intensive use of chemical fertilizers in arable farming dramatically increased environmental pollution through anthropogenic ammonia (NH3) and greenhouse gaseous emissions. Therefore, there is a need to develop improved fertilizer management practices that can reduce these losses. An experiment was conducted to assess the mitigating effects of sole or combined application of zeolite with biochar on gaseous emissions from arable land. For this purpose, zeolite (clinoptilolite) was mixed with different doses of biochar (produced from Dalbergia Sissoo wood chips) and applied along with the recommended dose of chemical fertilizer (NPK @ 150, 100, and 60 kg ha-1, respectively) on arable land in years 2013-14 and 2014-15. Immediately after application, these were incorporated into the top 10 cm of the soil layer and wheat was sown. Treatments were as follows: C = control, Z = zeolite @ 5 t ha-1, B1Z = biochar @ 3 t ha-1 + zeolite @ 5 t ha-1, B2Z = biochar @ 6 t ha-1 + zeolite @ 5 t ha-1, and B3Z = biochar @ 9 t ha-1 + zeolite @ 5 t ha-1. The experiment was laid out in a randomized complete block design (RCBD) with three replicates. The experimental plot size was 6 m × 4 m. Randomly, ten soil samples from each plot were taken at a depth of 0-15 cm and mixed to get a composite sample. All the samples were immediately stored in a freezer at -18°C until gaseous analysis in order to prevent N transformations. Each soil sample was analyzed for emission of NH3, CO2, and CH4 by using a selected-ion flow-tube mass spectrometer (SIFT-MS). Co-application of zeolite and biochar reduced NH3 and CH4 emissions by an average of 87 and 58% compared to the control, respectively. However, CO2 emission was increased by 104% relative to the control. The NH3 emission was decreased by an average of 61, 78, 90, and 92% by Z, B1Z, B2Z, and B3Z treatments compared to the control. Similarly, the decrement in CH4 emission was 47, 54, 55, and 65%. In contrast, the increment in CO2 emission was 42, 110, and 160% for B1Z, B2Z, and B3Z, respectively, while interestingly, a reduction of 12% was observed in Z treatment. Besides, co-application of zeolite and biochar at the highest dose (B3Z) improved soil chemical properties such as soil EC, OM, total N, as well as available P and K relative to zeolite alone. It is concluded that the combined application of zeolite and biochar can mitigate NH3 and greenhouse emissions and improve soil chemical characteristics, thus enhancing the environmental worth of arable farming.