An investigation on antimicrobial and anticancer competence of macro red algae under in-vitro condition.

The purpose of this study was to look into the proximate parameters (moisture, ash, total fat, protein, and total carbohydrate), mineral composition (Fe, Cu, Mg, and Zn), antimicrobial as well as cytotoxic (anticancer) properties of extracts from the marine red macro algae Gracilaria corticata, Chondrus ocellatus, and Posphyra perforata against a few prevalent microbial pathogens (Salmonella typhi, Streptococcus pneumoniae, Corynebacterium diphtheriae, Clostridium tetani, and Treponema pallidum as well as fungal pathogens such as Candida albicans, Aspergillus niger, and Cryptococcus neoformans) and two cancerous cell lines (HeLa and MCF7). The dry biomass of these red algae biomass contains considerable valuable proximate parameters and minerals. The diffusion technique on agar wells was used to evaluate the antimicrobial properties of these test red algae methanol and hexane extract; MTT assay was used to evaluate the cytotoxic effects of the methanol and hexane extracts on each cancer cell line. The methanol extracts demonstrated significant antimicrobial activity against most of the tested pathogenic organisms. Mortality of cells was effectively caused by methanol extract and it followed by hexane extract at increased dosage 10 mg mL-1. The MTT assay revealed that the methanol extract of the red algae was considerably cytotoxic to HeLa and MCF7 cells, accompanied by the hexane extract in a dose-dependent manner. These findings suggest that the methanol extract of these red algae may contain bioactive compounds with antimicrobial and anticancer properties, which could be studied for future use in the discovery of new drugs from marine ecosystems.

Caralluma pauciflorabased Ag-NPs activate ROS - induced apoptosis through down-regulation of AKT, mTOR and pI3K signaling in human gastric cancer (AGS) cells.

The phytochemicals found inCaralluma pauciflorawere studied for their ability to reduce silver nitrate in order to synthesise silver nanoparticles (AgNPs) and characterise their size and crystal structure. Thunbergol, 1,1,6-trimethyl-3-methylene-2-(3,6,9,13-tetram, Methyl nonadecanoate, Methyl cis-13,16-Docosadienate, and (1R,4aR,5S)-5-[(E)-5-Hydroxy-3-methylpent were the major compounds identified in the methanol extract by gas chromatography-mass spectrum analysis. UV/Vis spectra, Fourier-transform infrared spectroscopy, x-ray diffraction, scanning electron microscope with Energy Dispersive Xâray Analysis (EDAX), Dynamic Light Scattering (DLS) particle size analyser and atomic force microscope (AfM) were used to characterise theCaralluma paucifloraplant extract-based AgNPs. The crystal structure and estimated size of the AgNPs ranged from 20.2 to 43 nm, according to the characterization data. The anti-cancer activity of silver nanoparticles (AgNPs) synthesised fromCaralluma paucifloraextract. The AgNPs inhibited more than 60% of the AGS cell lines and had an IC50 value of 10.9640.318 g, according to the findings. The cells were further examined using fluorescence microscopy, which revealed that the AgNPs triggered apoptosis in the cells. Furthermore, the researchers looked at the levels of reactive oxygen species (ROS) in cells treated with AgNPs and discovered that the existence of ROS was indicated by green fluorescence. Finally, apoptotic gene mRNA expression analysis revealed that three target proteins (AKT, mTOR, and pI3K) were downregulated following AgNP therapy. Overall, the findings imply that AgNPs synthesised from Caralluma pauciflora extract could be used to treat human gastric cancer.

Compositional profiling and sensory analysis of cauliflower by-products-enriched muffins.

Cauliflower (Brassica oleracea var. botrytis) by-products (leaves, stems, stalks) (CBP) were successfully utilized in muffins as a model system and their feasibility of incorporation was investigated. CBP powder-based muffin formulations were made by the progressive replacement of wheat flour (WF) with 10%, 20%, and 30% of CBP. The physicochemical, pasting properties, antioxidant potential, textural characteristics, and sensorial attributes were analyzed. Substitution of CBP significantly (p < .05) resulted in an upsurge in crude protein, crude fiber, minerals, total phenolics, and total flavonoid contents, as well as total antioxidant activity values of muffins. The pasting properties were influenced by monitoring an increase in peak, breakdown, final, and setback viscosities. Although the addition of an increasing amount of CBP improved the nutritional characteristics, however, the increased level of replacement (>10%) had significant adverse effects on baking and physical characteristics. The specific loaf volume of the developed muffins decreased the crumb color which became darker, and enriched muffins were hardened in texture. Furthermore, sensory evaluation confirmed the positive effects of CBP incorporation only up to 10%. Overall, present results highlighted that supplementation of wheat muffins with 10% CBP is a beneficial approach to enrich them with nutrients and intensify their antioxidant potential.

Bio/phyremediation potential of Leptospirillum ferrooxidans and Ricinus communis on metal contaminated mine sludge.

The heavy metal pollution is a serious environmental pollution around the globe and threatens the ecosystem. The physicochemical traits (pH, Electrical conductivity, hardness, NPK, Al, Fe, Cd, Cr, Pb, Mg, and Mn) of soil sample collected from the polluted site were analyzed and found that the most of the metal contents were beyond the acceptable limits of national standards. The metals such as Mn (1859.37 ± 11.25 mg kg-1), Cd (24.86 ± 1.85 mg kg-1), Zn (795.64 ± 9.24 mg kg-1), Pb (318.62 ± 5.85 mg kg-1), Cr (186.84 ± 6.84 mg kg-1), and Al (105.84 ± 5.42 mg kg-1) were crossing the permissible limits. The pre-isolated L. ferrooxidans showed considerable metal tolerance to metals such as Al, Cd, Cr, Pb, Mg, and Mn at up to the concentration of 750 µg mL-1 and also have remediation potential on polluted soil in a short duration of treatment. The greenhouse study demonstrated that the bio/phytoremediation potential of metal tolerant L. ferrooxidans and R. communis under various remediation (A, B, and C) groups. Surprisingly, remediation group C demonstrated greater phytoextraction potential than the other remediation groups (A and B). These results strongly suggest that coexistence of L. ferrooxidans and R. communis had a significant positive effect on phytoextraction on metal-contaminated soil.

Optimization parameters for the production of dimer of epicatechin from an endophytic fungus Curvularia australiensis FC2AP using response surface methodology (RSM).

The search for natural therapeutic agents has intensified due to their potential to treat various diseases. Bioactive secondary metabolites from endophytes offer high therapeutic profiles and can be mass-produced after optimizing medium parameters and purification. This investigation aimed to maximize crude pigmented secondary metabolite (CPSM) production from Curvularia australiensis FC2AP by optimizing fermentation conditions statistically. The endophytic fungus produced a maximum yield of 8.81 UL/g from biomass using Sabouraud's Dextrose Broth. After screening essential factors, the Plackett-Burman design was used for factorial optimization, and the Box Behnken design was employed to investigate three significant factors. The final CPSM yield was 12.3 UL/g, approximately 4-fold higher than the preliminary growth medium. Chromatographic purification using a gradient solvent system resulted in six fractions, with the fourth fraction demonstrating the highest bioactivity profile. Structural characterization confirmed this fraction to be a dimer of epicatechin, which has anti-cancer properties, as confirmed through in vivo studies on Sprague Dawley rats. This is the first report of a epicatechin dimer produced from C. australiensis.

Larvicidal and pupicidal activity of phyto-synthesized zinc oxide nanoparticles against dengue vector aedes aegypti.

This study aimed to assess the phyto-synthesizing potential of Tarenna asiatica methanol leaf extract as well as its larvicidal and pupicidal potential against Aedes aegypti larvae. According to the findings of this study, the methanol leaf extract of T. asiatica has the potential to synthesize zinc oxide nanoparticles from zinc acetate dehydrate. Standard analytical techniques such as UV-visible spectrophotometer, Fourier-transform infrared spectroscopy, X-ray Diffraction analysis, Scanning Electron Microscope, and Energy Dispersive X-Ray were used to characterize the phyto-synthesized nanoparticles. The zinc oxide nanoparticles synthesized ranged in size from 22.35 to 31.27 nm and was spherical in shape. These nanoparticles demonstrated excellent larvicidal activity against Aedes aegypti larvae in the second, third, and fourth in stars, as well as significant pupicidal activity. These findings suggest that the methanol leaf extract of T. asiatica synthesized zinc oxide nanoparticles, which could be used to develop mosquito repellents.

An Image Processing Approach for Detection of Prenatal Heart Disease.

Prenatal heart disease, generally known as cardiac problems (CHDs), is a group of ailments that damage the heartbeat and has recently now become top deaths worldwide. It connects a plethora of cardiovascular diseases risks to the urgent in need of accurate, trustworthy, and effective approaches for early recognition. Data preprocessing is a common method for evaluating big quantities of information in the medical business. To help clinicians forecast heart problems, investigators utilize a range of data mining algorithms to examine enormous volumes of intricate medical information. The system is predicated on classification models such as NB, KNN, DT, and RF algorithms, so it includes a variety of cardiac disease-related variables. It takes do with an entire dataset from the medical research database of patients with heart disease. The set has 300 instances and 75 attributes. Considering their relevance in establishing the usefulness of alternate approaches, only 15 of the 75 criteria are examined. The purpose of this research is to predict whether or not a person will develop cardiovascular disease. According to the statistics, naïve Bayes classifier has the highest overall accuracy.

Synthesis of HAp/CS-SA composite for effective removal of highly toxic dyes in aqueous solution.

Textile industry is the major backbone of economy of the developing countries. The major problems associated in the textile factories are release of undesired dye effluents, which is a potential pollution risk for human health as well as the environmental aquatic system. The objective of this study was fabrication of a novel composite to treat textile industry effluents in an ecofriendly manner. In this context, hydroxyapatite (HAp) was derived from the mussel shell biowaste and fabricated with chitosan-sodium alginate through the in-situ method. The prepared HAp/CS-SA composite was physicochemically characterized by using Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FESEM-EDX) and High Resolution-Transmission Electron Microscope (HR-TEM). The photocatalytic activity of the HAp/CS-SA composite was evaluated by using Congo red (CR), Malachite green (MG) and Methylene blue (MB) as model dyes for degradation in aqueous solutions under solar irradiation. The degradation rate was recorded as CR (88%), MG (81%) and MB (93%) respectively within 75 min of irradiation. The degraded end products were subjected to toxicity assessment by evaluating the phytotoxicity on seed germination of Vigna radiata plant in pot study. The degraded end products were also tested for toxic inhibition of E. coli and P. aeruginosa by agar well diffusion method. The prepared HAp/CS-SA composite had an enhanced photocatalytic activity due to the presence of natural biopolymers and their biological properties. HAp/CS-SA composite showed potential dye degradation properties and it could be effective for dye removal from industrial wastewaters.

In vitro analysis of green synthesized copper nanoparticles using Chloroxylon swietenia leaves for dye degradation and antimicrobial application.

Green fabrication of copper nanoparticles (CuNPs) is an environmentally friendly and cost-effective method of synthesis for biomedical and bioremediation applications. In recent times, bacterial pathogens contaminating or affecting food and food crops pose the greatest threat to the food industry. In addition to this issue, synthetic dyes released from the textile and dyeing industries are polluting aquatic ecosystems and agricultural lands. The combined impact of these two factors is considered a major threat to life. Therefore, the use of CuNPs will provide an effective and long-term solution as an antibacterial and dye removing agent. The current study focuses on the synthesis of CuNPs using the leaf extract of Chloroxylon swietenia (C-CuNPs). The formation of a peak at 390 nm and a change in color from yellow to dark brown confirmed the synthesis of C-CuNPs. Subsequent synthesis at pH 9 was suitable for preparing C-CuNPs. Structural and chemical characterization of C-CuNPs was performed using Fourier Transfer Infra-Red (FTIR), X-ray diffraction (XRD), Dynamic Light scattering (DLS), and Scanning Electron Microscopy (SEM) analysis. The synthesized C-CuNPs possess a crystalline nature, a functional group that resembles C. swietenia, and are negatively charged and spherical in shape. C-CuNPs were tested against Congo red, Coomassie blue, and crystal violet and they showed complete degradation within 24 h under optimum conditions. Disk diffusion and broth dilution assay were used to test the antibacterial activity of C-CuNPs against Staphylococcus nepalensis, Staphylococcus gallinarum, Pseudomonasstutzeri,Bacillus subtilis, and Enterococcus faecalis. Therefore, the present study represents the first report on C-CuNPs' ability to degrade synthetic dyes and kill foodborne bacterial pathogens. Thus, the study has shed light on the potential of green synthesized CuNPs as bioremediation and packaging material in the future.

Development of a Model System to Study Expression Profile of RAC2 Gene in Breast Cancer MDA-MB-231 Cell Line.

The RAC2 gene encoding GTPases involve cellular signaling of actin polymerization, cell migration, and formation of the phagocytic NADPH oxidase complex. Oncogenic mutations in the RAC2 gene have been identified in various cancers, and extensive research is in progress to delineate its signaling pathways and identify potential therapeutic targets in breast cancers. This paper explored developing a bioinformatics model system to understand the RAC2 gene expression pattern concerning estrogenic receptor status in breast cancers. We have used the MDA-MB-231 breast cancer cell line to identify RAC2 gene expression. To simplify the development of model system with one dataset, we retrieved the microarray dataset GSE27515 from the Gene Expression Omnibus (GEO) for the differential gene expression analysis. Then, network analysis, pathway enrichment analysis, volcano plot, ORA, and the up/downregulated genes were used to highlight genes involved in signaling network pathways. We observed that the RAC2 gene is upregulated in the GSM679722, GSM676923, and GSM679724 downregulated in the samples GSM676925, GSM676926, and GSM676927 from the GEO dataset. Our observation found that the RAC2 gene is upregulated in the estrogen receptor (ER) negative breast cancers and downregulated in ER-positive breast cancer, involving pathways such as focal adhesion, MAPK signaling, axon guidance, and VEGF signaling pathway.

Isolation, Identification, Characterization, and Plasmid Profile of Urinary Tract Infectious Escherichia coli from Clinical Samples.

Objective: In recent times, urinary tract infection (UTI) is one of the most widely recognized bacterial diseases all over the planet. UTI influences individuals of any age and gender. The target of this study is to concentrate on the recurrence of uropathogens, the antimicrobial susceptibility pattern of the isolates, and the plasmid profile of people from the government clinics of Karaikudi. Methods: From July 2017 to December 2017, 100 urine tests were gathered and handled for the isolation of pathogenic microbes. In total, 89 isolates were found from the samples collected. Results: Escherichia coli was discovered as the most common bacterial isolate screened from the UTI-infected people, accounting for 28.09 percent of all isolates. E. coli was seen to be the highest prevalent bacterium for UTI in all age groups and demonstrated resistance to routinely used medications, especially cefpodoxime and novobiocin, which have been 100 percent resistant. The E. coli isolates screened were positive for beta-lactamase and film generation, and they have strong antimicrobial resistance. As a result, the E. coli strains with the highest prevalence of virulence determinants have become more resistant to many medications because they support the microorganism in overcoming the host's defense and colonizing or entering the urinary system. The amplified 16S rRNA product was analyzed, and phylogenetic relationships were determined. The presence of TEM (56 percent), CTX-M (64 percent), SHV (40 percent), and OXA (60 percent) was discovered. Among E. coli isolates, CTX-M was the most common extended spectrum-beta lactamase (ESBL). Multiplex PCR was also used to identify the existence of CTX-M subgroups in E. coli isolates. Conclusion: Finally, we urge that antibiotic selection should be predicated on the awareness of the specific prevalence and that novel antimicrobial medicines for urinary infections be developed to combat the overuse of antibiotics.

Antibacterial properties of Apis dorsata honey against some bacterial pathogens.

Now-a-days, different bioproducts are being used extensively for the welfare of mankind. However, for proper utility of any bioproduct, the exact biotechnological potential of that product should be explored. Honey is produced in almost every country on the planet. It has long been used as a medicinal agent in addition to its broader use as a popular food throughout the human history. It can be used to treat various diseases without causing any negative side effects. In the present study, the antibacterial potential of honey produced by A. dorsata was investigated at its variable concentrations (25, 50, 75 and 100 %) against four pathogenic bacterial species. The highest antimicrobial action was seen against E. coli at 100 % concentration of the honey while showing zone of inhibition of 37.5 ±â€¯3.5 mm. However, the lowest antibacterial action was observed against E. faecalis. The overall order of growth inhibition by the honey at its 100 % concentration for the implicated bacterial species appeared as: E. coli ˃ P. aeruginosa ˃ S. aureus ˃ E. faecalis. The honey couldn't show antibacterial action at its 25 % concentration. Our findings of the present study will be helpful for utility of the honey as an alternative medicine for curing different complications caused by microbial pathogens.

Anticancer and Free Radical Scavenging Competence of Zinc Oxide Nanoparticles Synthesized by Aqueous Leaf Extract of Phyllanthus acidus.

The purpose of this study was aimed to investigate the zinc oxide nanoparticles (ZnONPs) synthesizing efficiency of aqueous leaf extract of Phyllanthus acidus. Furthermore, the antioxidant and anticancer activities of synthesized ZnONPs were also investigated through the in-vitro approach. The obtained results show that the aqueous extract of P. acidus can synthesize ZnONPs, as evidenced by a sharp absorbance peak at 375 nm. The Fourier transform infrared spectroscopy (FTIR) analysis confirmed that the aqueous extract contained significant numbers of functional groups, which were involved in reducing zinc nitrate into ZnONPs. Also, they participate in the capping and stabilization of synthesized ZnONPs and their size ranged from 27.14-35.74 nm with a spherical shape . The results obtained in ABTS radical scavenging activity 1, 1-diphenyl-2-picryl-hydroxyl (DPPH), hydrogen peroxide (H2O2), and 2,2'-Azino-Bis(3-ethylbenzene thiazoline-6-sulfonic acid) (ABTS) assays declared has excellent in-vitro radicals scavenging activity with reasonable IC50 values. Interestingly, these green synthesized ZnONPs have an excellent anticancer activity against human epidermoid carcinoma (Hep3) cell line in an in-vitro approach. These findings imply that an aqueous leaf extract of P. acidus can be used to synthesize pharmaceutically valuable ZnONPs. To consider such nanomaterials as potential therapeutic agents, optimization and in-vivo biomedical studies are required.

Marine Macroalgae Display Bioreductant Efficacy for Fabricating Metallic Nanoparticles: Intra/Extracellular Mechanism and Potential Biomedical Applications.

The application of hazardous chemicals during nanoparticle (NP) synthesis has raised alarming concerns pertaining to their biocompatibility and equally to the environmental harmlessness. In the recent decade, nanotechnological research has made a gigantic shift in order to include the natural resources to produce biogenic NPs. Within this approach, researchers have utilized marine resources such as macroalgae and microalgae, land plants, bacteria, fungi, yeast, actinomycetes, and viruses to synthesize NPs. Marine macroalgae (brown, red, and green) are rich in polysaccharides including alginates, fucose-containing sulfated polysaccharides (FCSPs), galactans, agars or carrageenans, semicrystalline cellulose, ulvans, and hemicelluloses. Phytochemicals are abundant in phenols, tannins, alkaloids, terpenoids, and vitamins. However, microorganisms have an abundance of active compounds ranging from sugar molecules, enzymes, canonical membrane proteins, reductase enzymes (NADH and NADPH), membrane proteins to many more. The prime reason for using the aforesaid entities in the metallic NPs synthesis is based on their intrinsic properties to act as bioreductants, having the capability to reduce and cap the metal ions into stabilized NPs. Several green NPs have been verified for their biocompatibility in human cells. Bioactive constituents from the above resources have been found on the green metallic NPs, which has demonstrated their efficacies as prospective antibiotics and anti-cancer agents against a range of human pathogens and cancer cells. Moreover, these NPs can be characterized for the size, shapes, functional groups, surface properties, porosity, hydrodynamic stability, and surface charge using different characterization techniques. The novelty and originality of this review is that we provide recent research compilations on green synthesis of NPs by marine macroalgae and other biological sources (plant, bacteria, fungi, actinomycetes, yeast, and virus). Besides, we elaborated on the detailed intra- and extracellular mechanisms of NPs synthesis by marine macroalgae. The application of green NPs as anti-bacterial, anti-cancer, and popular methods of NPs characterization techniques has also been critically reviewed.

Kaolin and Jasmonic acid improved cotton productivity under water stress conditions.

Drought is one of the most emerging threat that causes a severe reduction in cotton plant growth and development. Being cotton is a major cash crop has great threat to prevailing drought events in Pakistan. A field experiment was conducted in Kharif season 2018 at Research Area of MNS-University of Agriculture, Multan, Pakistan to assess the role of foliar applied kaolin and jasmonic acid on vegetative growth, gas exchange and reproductive traits of cotton under normal irrigated and artificial water deficit conditions. The experiment was laid -out in a factorial randomized complete block design with split - split plot arrangement. Main plots were allocated for irrigation levels, sub-plots for two -cotton genotypes viz. NIAB - 878 and SLH - 19 while sub - sub plots for treatments of kaolin and Jasmonic acid. Water deficit stress was created by skipping irrigation at flowering for 21 days. Foliar sprays of Kaolin (5%, w/v) and Jasmonic acid (100 µM) were applied alone or in combination at 60 days after planntinon both to normal irrigated and water-stresse skip irrigation while irrigation water alone was sprayed in control plots. Both cotton genotypes responded variably to normal irrigated and skip conditions. Skipping irrigation for up to 21 days at flowering caused a significant decrease in leaf relative water content, SPAD values, net photosynthetic rate and seed cotton yield in both the genotypes. Seed cotton yield showed an overall decline of 24.7% in skip over Normal irrigated crop. The genotype NIAB - 878 produced maximum seed cotton yield of 3.304 Mg ha-1 in normal that dropped to 2.579 Mg ha-1 in skip, thus showing an average decline of 21.9 %. Similarly, SLH - 19 produced 2.537 Mg ha-1 seed cotton under normal that dropped to 1.822 Mg ha-1 in skip, showing an average decline of 28.2%. The Application of Kaolin and JA Jasmonic acid, either applied individually or in combination, improved vegetative and reproductive development of both cotton varieties in normal and skip regimes. However, combined kaolin and Jasmonic Acid application proved to be more beneficial in terms of seed cotton production and other parameters studied.

Pyrimidine-2,4-dione targets STAT3 signaling pathway to induce cytotoxicity in hepatocellular carcinoma cells.

Signal transducer and activator of transcription 3 (STAT3) is a tumorigenic transcription factor that is persistently activated in various human cancers including hepatocellular carcinoma (HCC). Therefore, STAT3 is considered as a prominent target to counteract the uncontrolled proliferation of cancer cells. In the present report, pyrimidine-2,4-diones (N-methyluracil derivatives) (MNK1-MNK14) were synthesized in an ionic liquid (BMIm PF6) medium employing a ligand-free Suzuki-Miyaura cross-coupling process. Among the 14 derivatives, compound MNK8 showed good cytotoxicity towards both the tested cell lines and did not display a toxic effect against normal hepatocytes (LO2). MNK8 significantly increased the Sub-G1 cell count in both cell lines and the cytotoxic effect of MNK8 was found to be mediated through the suppression of constitutive phosphorylation of STAT3Y705. It also decreased the DNA interaction ability of nuclear STAT3 in HCC cells. MNK8 downregulated the levels of apoptosis-related proteins (such as Bcl-2, cyclin D1, survivin) and increased cleaved caspase-3 inferring the apoptogenic effect of MNK8. It also reduced the CXCL12-triggered cell migration and invasion in in vitro assay systems. Overall, MNK8 has been demonstrated as a new inhibitor of STAT3 signaling cascade in HCC cells.

Biogenesis of copper nanoparticles (Cu-NPs) using leaf extract of Allium noeanum, antioxidant and in-vitro cytotoxicity.

In this research, we formulated new chemotherapeutic copper nanoparticles (Cu NPs) containing Allium noeanum Reut. ex Regel leaf for treating human endometrial cancer. For investigating the antioxidant activitiy, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was used. MTT test was used on normal (Human umbilical vein endothelial cells (HUVECs)) and human endometrial cancer (Ishikawa, HEC-1-A, HEC-1-B, and KLE) cell lines for comparing the anti-human endometrial cancer properties of Cu(NO3)2, A. noeanum leaf aqueous extract, and copper nanoparticles. Copper nanoparticles had high cell death and anti-human endometrial cancer effects against Ishikawa, HEC-1-A, HEC-1-B, and KLE cell lines. The IC50 of A. noeanum leaf aqueous extract and copper nanoparticles against HEC-1-B cell line were 548 and 331 µg/mL, respectively; against HEC-1-A cell line were 583 and 356 µg/mL, respectively; against KLE cell line were 609 and 411 µg/mL, respectively; and against Ishikawa cell line were 560 and 357 µg/mL, respectively. Among the above cell lines, the best result of anti-human endometrial cancer properties of copper nanoparticles was gained in the cell line of HEC-1-B. This study indicated excellent anti-human endometrial cancer potentials of copper nanoparticles containing A. noeanum in the in vitro condition.

Impact of anti-sandfly saliva antibodies on biological aspects of Phlebotomus papatasi (Diptera: Psychodidae), vector of cutaneous leishmaniasis.

Sandflies are the main vectors of Leishmania parasites in tropical and subtropical areas. The immunization of vertebrate hosts with vector components through repeated bites may offer an alternative method for sandfly control. Aliquots of female Phlebotomus papatasi (Scopoli) (Diptera: Psychodidae) were weekly blood fed on 12 individual hamsters throughout 18 successive weeks. Significant biological and biochemical changes resulting from antibodies developed by immunized host sera against repeated biting were observed in sandfly females. Blood feeding and fertility rates of females significantly gradually declined to the end of the study period. No appreciable difference was observed in mortality rates among flies repeatedly fed on individual hamsters throughout weeks 9 and 18, compared to flies fed on naïve hamsters. Total salivary gland proteins of female sandflies were compared to proteins in sera of sensitized hamsters. SDS-page revealed bands common to both flies and hosts, indicating the development of anti-saliva antibodies in hamster sera. The importance of anti-sandfly saliva antibodies as a potential tool for vector control leading to the interruption of leishmaniasis is discussed.

Growth performance of Ganoderma lucidum using billet method in Garhwal Himalaya, India.

Medicinal mushrooms have been used in various treatments from a very long time, among which, Ganoderma lucidum is one of the most important medicinal mushroom. It is cultivated worldwide to meet its ever-increasing demand in the market. It is generally cultivated by bed log (Sawdust) and wood log (billet) method. This study was an attempt to observe the growth performance of G. lucidum on poplar billets (Populus deltoides) in the Sherpur Village (Dehradun) and Manjgaun village (Tehri Garhwal) of Garhwal Himalaya, India. The farmers' field with empty house/ rooms having proper growing conditions especially humidity and light were used for the cultivation of G. lucidum. The G. lucidum spawn was inoculated in poplar wood billets and these billets were installed in well prepared soil. The results demonstrated that cropping cycle of G. lucidum was shorter (132-136 days) in Sherpur Village (Dehradun) as compared to Manjgaun village (141-145 days) in Tehri Garhwal. Further the results also revealed that yield was decreased in the subsequent flushes. In Village Sherpur, the fruiting bodies of G. lucidum were harvested between 64-66 days, 100-101  days and 135-136  days during first, second and third flush after the installation of billets, respectively. However; in village Manjgaun, the fruiting bodies of G. lucidum were harvested between 69 and 71 days, 107-108  days and 144-145 days in first, second and third after the installation of billets respectively. Warmer temperature in Village Sherpur resulted in the early emergence and development of the fruiting bodies as compared to village Manjgaun where pinhead and fruiting body development was delayed due to the lower temperature during cropping cycle.

Assessment of risk management and control measures against coronavirus disease.

This article presents the COVID-19 situation and control measures taken by the Government of Pakistan. Two waves of pandemic are faced globally and similar in the study area. We have investigated the risk management decision in two phases. Primarily, strict lockdown was observed from March 2020 to July 2020 and smart lockdown was enforced from August 2020 to December 2020. It has been studied that during strict lockdown, COVID cases reduced gradually but reopening of institutes and smart lockdown strategy resulted gradual increase in confirmed cases and death rates. During first wave of COVID-19 in Pakistan, a total confirmed number of patients of COVID-19 were 263,496 till 18th of July 2020 with total deaths of 5,568 people and 204,276 recoveries, while total number of COVID-19 patients reached 555,511 till 9th of February 2021 with total deaths of 12,026 people. Province of Sindh was affected badly with total number of 251,434 COVID-19 cases followed by Punjab Province with total number of 161,347 COVID-19 till 9th of February 2020.