Detection of non-melanoma skin cancer by in vivo fluorescence imaging with fluorocoxib A.

Non-melanoma skin cancer (NMSC) is the most common form of cancer in the US and its incidence is increasing. The current standard of care is visual inspection by physicians and/or dermatologists, followed by skin biopsy and pathologic confirmation. We have investigated the use of in vivo fluorescence imaging using fluorocoxib A as a molecular probe for early detection and assessment of skin tumors in mouse models of NMSC. Fluorocoxib A targets the cyclooxygenase-2 (COX-2) enzyme that is preferentially expressed by inflamed and tumor tissue, and therefore has potential to be an effective broadly active molecular biomarker for cancer detection. We tested the sensitivity of fluorocoxib A in a BCC allograft SCID hairless mouse model using a wide-field fluorescence imaging system. Subcutaneous allografts comprised of 1000 BCC cells were detectable above background. These BCC allograft mice were imaged over time and a linear correlation (R(2) = 0.8) between tumor volume and fluorocoxib A signal levels was observed. We also tested fluorocoxib A in a genetically engineered spontaneous BCC mouse model (Ptch1(+/-) K14-Cre-ER2 p53(fl/fl)), where sequential imaging of the same animals over time demonstrated that early, microscopic lesions (100 µm size) developed into visible macroscopic tumor masses over 11 to 17 days. Overall, for macroscopic tumors, the sensitivity was 88% and the specificity was 100%. For microscopic tumors, the sensitivity was 85% and specificity was 56%. These results demonstrate the potential of fluorocoxib A as an in vivo imaging agent for early detection, margin delineation and guided biopsies of NMSCs.

Phonon dispersion and heat capacity in polyfuran.

A study of the normal modes of vibration and their dispersion in polyfuran (Pfu) based on the Urey-Bradley force field is reported. It provides a detailed interpretation of IR and Raman spectra. Characteristic features of dispersion curves such as regions of high density-of-states, repulsion and character mixing of dispersive modes are discussed. Predictive values of heat capacity as a function of temperature are calculated.

Resonant sum frequency generation with time-energy entangled photons.

We describe a technique for enhancing the efficiency of sum frequency generation using entangled signal and idler photons. By resonating the sum frequency field, we observe that the generated power varies linearly with input power and is increased by a factor of 12.

All-optical delay of images using slow light.

Two-dimensional images carried by optical pulses (2 ns) are delayed by up to 10 ns in a 10 cm cesium vapor cell. By interfering the delayed images with a local oscillator, the transverse phase and amplitude profiles of the images are shown to be preserved. It is further shown that delayed images can be well preserved even at very low light levels, where each pulse contains on average less than one photon.

Large-alphabet quantum key distribution using energy-time entangled bipartite States.

We present a protocol for large-alphabet quantum key distribution (QKD) using energy-time entangled biphotons. Binned, high-resolution timing measurements are used to generate a large-alphabet key with over 10 bits of information per photon pair, albeit with large noise. QKD with 5% bit error rate is demonstrated with 4 bits of information per photon pair, where the security of the quantum channel is determined by the visibility of Franson interference fringes. The protocol is easily generalizable to even larger alphabets, and utilizes energy-time entanglement which is robust to transmission over large distances in fiber.