An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis.

Microbial production of succinic acid (SA) at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation for over three decades. Here, we metabolically engineer the acid-tolerant yeast Issatchenkia orientalis for SA production, attaining the highest titers in sugar-based media at low pH (pH 3) in fed-batch fermentations, i.e. 109.5 g/L in minimal medium and 104.6 g/L in sugarcane juice medium. We further perform batch fermentation using sugarcane juice medium in a pilot-scale fermenter (300×) and achieve 63.1 g/L of SA, which can be directly crystallized with a yield of 64.0%. Finally, we simulate an end-to-end low-pH SA production pipeline, and techno-economic analysis and life cycle assessment indicate our process is financially viable and can reduce greenhouse gas emissions by 34-90% relative to fossil-based production processes. We expect I. orientalis can serve as a general industrial platform for production of organic acids.

Personal Intelligence Is Evident in the Sophistication of People's Narratives about Personality.

Personal intelligence concerns the ability to understand personality in oneself and others-including the understanding of motives, socioemotional traits, and abilities. We examined if people's scores on the ability-based Test of Personal Intelligence (TOPI) would be reflected in their narratives about someone whose personality they had learned about. In a Preliminary Study (N = 220), we collected narratives and open-ended descriptions about their learning. In Study 1 (N = 212), experts rated the respondents' open-ended narratives for their sophistication about personality, defined as their knowledge and complexity of thought around the topic. Respondents also filled out checklists concerning what they learned and their relationship outcomes. Study 2 (N = 299) was a replication and extension in which we added the TOPI. Participants who scored higher on the TOPI produced narratives higher in Sophistication, even after statistical controls for Word Count and Vocabulary (the measures also were largely independent of the Big Five). The findings here may have applications for both testing and training.