Mercury distribution, bioaccumulation, and biomagnification in riparian ecosystems from a neotropical savanna floodplain, Araguaia River, central Brazil.

Litterfall is the main source of dry deposition of mercury (Hg) into the soil in forest ecosystems. The accumulation of Hg in soil and litter suggests the possibility of transfer to terrestrial invertebrates through environmental exposure or ingestion of plant tissues. We quantified total mercury (THg) concentrations in two soil layers (organic: 0-0.2 m; mineral: 0.8-1 m), litter, fresh leaves, and terrestrial invertebrates of the Araguaia River floodplain, aiming to evaluate the THg distribution among terrestrial compartments, bioaccumulation in invertebrates, and the factors influencing THg concentrations in soil and invertebrates. The mean THg concentrations were significantly different between the compartments evaluated, being higher in organic soil compared to mineral soil, and higher in litter compared to mineral soil and fresh leaves. Soil organic matter content was positively related to THg concentration in this compartment. The order Araneae showed significantly higher Hg concentrations among the most abundant invertebrate taxa. The higher Hg concentrations in Araneae were positively influenced by the concentrations determined in litter and individuals of the order Hymenoptera, confirming the process of biomagnification in the terrestrial trophic chain. In contrast, the THg concentrations in Coleoptera, Orthoptera and Hymenoptera were not significantly related to the concentrations determined in the soil, litter and fresh leaves. Our results showed the importance of organic matter for the immobilization of THg in the soil and indicated the process of biomagnification in the terrestrial food web, providing insights for future studies on the environmental distribution of Hg in floodplains.

Mercury in Fish and Human Hair and Estimated Dietary Intake in a Riverside Community of the Madeira River Basin in the Brazilian Amazon.

Mercury (Hg) is a chemical element that poses risks to human health due to its high toxicity and environmental persistence. We determined the total Hg (THg) and methyl Hg (MeHg) concentrations in hair samples from residents of the Demarcação District (Porto Velho, Rondônia) in the Brazilian Amazon, as well as in water and fish samples, to evaluate factors influencing human exposure. The average THg concentration in human hair was 7.86 ± 6.78 mg kg-1 and it was significantly higher in men, with an increasing trend related to age. There was no significant difference between female age groups. Human exposure to Hg through water was negligible compared to fish consumption. The average weekly intake estimates in the community varied between 1.54 and 4.62 µg kg-1, substantially higher than the recommended limit. The fish species with the highest amounts safe for daily consumption were herbivores and detritivores. Our results contribute to an understanding of how exposure to Hg affects the health of riverside populations and provide insights for new research to develop methods to mitigate such exposure and thus improve the quality of life of Amazonian people.

A 35-Year Record (1987-2022) of Hg Concentrations in Two of the Fish Species Most Consumed by People Living in the Upper Madeira River Basin, Brazilian Amazon Region.

This study presents a 35-year record of total mercury (Hg) concentrations in the detritivore fish Prochilodus nigricans (Curimatã) and the carnivore Cichla pleiozona (Tucunaré), two of the most widely distributed, ecologically important and consumed fish species in the upper Madeira River Basin in the Western Brazilian Amazon. Fish samples from the major Madeira River and marginal lakes and tributaries were compared. Irrespective of site, Hg concentrations were higher in the carnivore fish compared to the detritivore. Hg concentrations increased 5-fold in C. pleiozona in the past three decades, whereas they remained relatively constant in P. nigricans when analyzing the entire 35-year period. When analyzed separately, fish in the main river and marginal lake and tributaries presented the same pattern of Hg variation, with a significant increase in Hg concentrations in the carnivore and in the detritivore in marginal lakes and tributaries but not in the main river. This was in line with the increase in methyl-Hg production in tributaries, mostly associated with deforestation in the past decade in the basin. Although an increase in direct emissions from artisanal gold mining also occurred in the past decade, this caused virtually no impact on fish Hg concentrations, suggesting atmospheric emission and deposition in forests and further export to water systems as an intermediate link with fish Hg concentrations.

Evaluating total mercury and methylmercury biomagnification using stable isotopes of carbon and nitrogen in fish from the Madeira River basin, Brazilian Amazon.

The isotopic signatures of carbon in primary consumer fish species in the Madeira River basin (Western Amazon) follow a pattern, with basal species feeding mainly on C3 (e.g., vascular plants and phytoplankton). In this study with 196 specimens of 19 fish species and six trophic guilds, significant differences were found between the two main groups of primary consumers (herbivores and detritivores) in the Madeira River basin. The mercury and δ15N data indicated that the fish specimens collected in Puruzinho and Cuniã Lakes provided greater trophic magnification factors (TMFs) for this metal than those found in the corresponding rivers. In relation to total mercury (0.09-2.07 µg g-1) and methylmercury (0.05-1.91 µg g-1) concentrations, the piscivorous/carnivorous species exceeded the values recommended by the World Health Organization, with a [MeHg:THg] ratio of about 80%. Preventive and educational practices should be adopted to reduce the possible toxic effects of Hg in the riverside population, for whom fish constitutes the main protein source. Also, biomonitoring of the fish species in the Madeira River should be conducted on an ongoing basis to detect possible alterations in mercury concentrations.

Spatial-temporal dynamics of Cr in fish from Puruzinho Lake (Western Amazon) and dietary risk assessment.

Cr contamination is a growing concern in the Amazon. Along with the rise of cattle farming in the region, tanneries are becoming more common and threatening environmental quality, since the effluents from this activity are rich in Cr. The objective of this study was to evaluate the seasonal and spatial dynamics of Cr in fish with different feeding habits from Puruzinho Lake, located in the Madeira River basin, also determining the daily intake of Cr associated with the consumption of these fish by the population that lives around the lake. Seasonality and feeding habits directly affected Cr dynamics in fish (p < 0.0001), with medians of Cr concentration ranging between 0.07 mg kg-1 in the species Cichla pleiozona, a carnivorous fish, and 0.62 mg kg-1 in Mylossoma duriventre, a herbivorous fish. The high Cr concentrations in M. duriventre were related to the biodilution effect of Cr in the trophic chain (R2 = 0.14, p < 0.0001). The consumption of M. duriventre by the population was associated with the highest daily intake of Cr (55.5 µg∙day-1), exceeding the recommended limits of Cr intake (35 µg∙day-1). However, no harmful effects on health are expected due to the ingestion of Cr through the consumption of fish from Puruzinho Lake. Spatial analysis showed an increasing trend in Cr concentrations along the Madeira River, suggesting that contaminants present in the river are being carried over long distances and are reaching preserved areas of the Amazon.

Acidentes perfurocortantes entre acadêmicos de Odontologia / Accidents with cutting and piercing materials among Dentistry students

Os profissionais da saúde estão expostos aos mais diversos riscos ocupacionais durante a sua jornada de trabalhoeos cirurgiões-dentistas lideram o número de acidentes com materiais biológicos. Os atendimentos por acadêmicos de odontologia os deixam expostos aos riscos ocupacionais de forma semelhante aos profissionais da área. Este estudo teve o objetivo de avaliar prevalência e características dos acidentes com instrumentais perfurocortantesentre osacadêmicos de umcurso de Odontologia. Constatou-se prevalência de 28,85%de acidentes, com o maior percentual no 5° períodode curso,principalmente nasclínicas de Cirurgia e Endodontia. Apenas 20% dos afetados relataram a ocorrênciae a sonda exploradora foi o instrumental mais envolvido.Além disso,osacidentes ocorreram de forma igual no atendimento ao pacienteelavagem do instrumental. Portanto, faz-senecessário investimento em ações educativas e normativas permanentes para conscientização de acadêmicos sobre acidentes perfurocortantes (AU).

Health professionals are exposed to the most diverse occupational risks during their workday and dentists lead the number of accidents with biological materials. The assistance provided by dental students leaves them exposed to occupational risks in a similar way to professionals in the field. This study aimed to evaluate the prevalence and characteristics of accidents with cutting and piercing instruments among students of a Dentistry course. There was a prevalence of 28.85% of accidents, with the highest percentage in the 5th period of the course, mainly in the Surgery and Endodontics clinics. Only 20% of those affected reported the occurrence and the sickle probe was the instrument most involved. In addition, accidents occurred equally in patient care and washing of instruments. Therefore, it is necessary to invest in permanent educational and normative actions to raise the awareness of students about needlestick injuries (AU).

Mercury in blood, hair, and feces from subsistence fish-eating riverines of the Madeira River Basin (Western Amazon).

BACKGROUND: The Madeira River (Amazon Basin) has been impacted by activities related to artisanal and small-scale gold mining (ASGM), deforestation and burning (for timber, agriculture, and hydroelectric dam projects). All these activities contribute to environmental mercury (Hg) release and cycling into the Amazon ecosystem and thus to changing lifestyles. METHOD: We assessed exposure to total and MeHg in two small riverine communities of the Madeira River (Amazon): Lago Puruzinho (LP, n = 26 families) and São Sebastião do Tapurú (SST, n = 31 families). Samples of human hair (n = 137), blood (n = 39), and feces (n = 41) were collected from adults and children (0-15 years of age). RESULTS: In women of childbearing age from LP village, the mean blood total-Hg (THg) (45.54 ±â€¯24.76 µg.L-1) and MeHg (10.79 ±â€¯4.36 µg.L-1) concentrations were significantly (p = 0.0024; p < 0.0001, respectively) higher than in women from SST village (THg: 25.32 ±â€¯16.75 µg.L-1; MeHg: 2.32 ±â€¯1.56 µg.L-1) village; the trend in hair-Hg persisted but was statistically significant (p < 0.0145) only for THg (LP, 11.34 ±â€¯5.03 µg. g-1; SST, 7.97 ±â€¯3.51 µg. g-1). In women, the median hair:blood ratio of total Hg was 269. In children, the mean hair THg concentrations were 6.07 ±â€¯3.60 µg. g-1 and 6.47 ±â€¯4.16 µg. g-1 in LP and SST; thus, not significantly different (p = 0.8006). There was a significant association (p < 0.001) between hair-Hg concentrations of mothers and their respective children. The excretion of Hg in feces of women (0.52 µg. g-1 dw) was not significantly different from children (0.49 µg. g-1 dw). The only statistically significant correlation between Hg in feces and in hair was found in children, (n = 16, rs = 0.38, p = 0.005). Significant relationship was seen between the levels of THg in blood and hair of women from LP and SST. Based on hair-Hg concentrations, fish consumption rate ranged from 94.5 to 212.3 g.day-1. CONCLUSION: Women and children excrete THg in feces in comparable concentrations. However, the mean fish consumption rate and blood MeHg are higher in the most remote villagers. Mother`s hair-Hg concentration is a good predictor of children's hair-Hg.

Total mercury and methylmercury in river dolphins (Cetacea: Iniidae: Inia spp.) in the Madeira River Basin, Western Amazon.

In the Amazon, mercury (Hg) contamination comes from ASGM operations along with soil remobilization processes associated with deforestation. The objective of this study was to evaluate the exposure to methylmercury (MeHg) and total mercury (THg) in 88 samples of skin and blubber tissue obtained from live captured river dolphins (Inia boliviensis, Inia geoffrensis, and Inia spp.) in the Madeira River Basin. THg and MeHg measurements were performed by CV-AAS and GC-AFS, respectively. We also calculated the daily intake rate (DIR) of THg (wet weight) by Inia spp. THg levels in blubber tissue of adult river dolphins (Inia spp.) ranged from 0.015 to 3.804 mg kg-1, while MeHg concentrations in blubber tissue varied from 0.04 to 2.65 mg kg-1 and in skin tissue from 0.09 to 0.66 mg kg-1. There were no significant differences in MeHg concentration in blubber (p = 0.616) and skin (p = 0.498) tissue samples between adult males and females in the different sampling locations. The adult animals showed differences in THg and MeHg concentrations significantly higher than in the calves. The estimate of the DIR of the genus Inia ranged from 1.17 to 12.35 µg kg-1 day-1 (bw), from the consumption of fish species with herbivorous to piscivorous habits, respectively. More biological and ecological data, such as the precise determination of age, mediated length, weight, and diet of river dolphins, are necessary to verify the Hg biomagnification. However, our data indicate that bioaccumulation is an active process in the dolphins of the Madeira River Basin.

Dynamics of Hg and MeHg in the Madeira River basin (Western Amazon) before and after impoundment of a run-of-river hydroelectric dam.

The impact of a hydroelectric run-of-river (RoR) dam construction on the dynamics of total mercury (THg) and methylmercury (MeHg) is of interest to the environment and health of human and wild life. We monitored (May 2010 to October 2018) THg and MeHg in the waters and in the suspended particulate matter (SPM) of the Madeira River and its tributaries (before and after dam construction) to evaluate changes upstream and downstream from the Santo Antonio Hydroelectric Dam (SAHD). We collected 2826 samples of water and SPM at sampling stations upstream (UPMD-01, UPMD-02 and UPMD-03) and downstream the Madeira River (DWMD-04, DWMD-05 and DWMD-06), and Tributaries upstream (Branco River, Jaci-Parana River, Jatuarana-I Igarapé, Contra River, Caripunas Igarapé, Ceara Igarapé, and Teotonio Igarapé) and downstream (Jatuarana-II Igarapé and Belmont Igarapé) from the SAHD and monitored water and the total load of SPM. SPM was significantly higher in the Madeira River (median: 178.63 mg.L-1) than in upstream and downstream tributaries (median: 15.30 mg.L-1); however, the THg and MeHg concentrations were significantly higher in the tributaries (median: 161.14 ng g-1 and 9.03 ng g-1, respectively) than in the mainstream Madeira River (median = 57.06 ng g-1 and 1.63 ng g-1, respectively). THg concentration in the water was significantly higher in the mainstream Madeira River (median = 6.51 ng.L-1) than in the tributaries (median = 2.57 ng.L-1). However, the percentage of methylation in the tributaries (median = 4.9%) was 4-times higher than in the Madeira River (median: 1.3%). The significantly higher MeHg percentages in the tributaries may indicate natural (hydro-bio-geochemical factors) still predominant in this changing landscape of the Western Amazon. So far, the data suggest that this RoR has not yet impacted the THg and MeHg concentrations.

Association between maloclusion and self-perception of oral aesthetics in adolescents

Aim: To evaluate the aesthetic self-perception capacity of adolescents from public schools regarding the presence of oral alterations from malocclusions. Methods: Cross-sectional and quantitative study with 374 adolescents between 16 and 18 years old, belonging to public schools in the city of Parnaíba, Piauí. The adolescents were separated by gender (male and female) and examined for malocclusions. The problem identified for each participant was recorded for later comparison of the influence of its presence with aesthetic self-perception. Then, all adolescents, including those who demonstrated normal occlusion during the clinical evaluation, answered a questionnaire containing eight questions about their perception of their own smile and its impact on their interpersonal relationships. Self-perception was also analyzed by comparing the responses of those with normal occlusion with that of malocclusion individuals. Student's t-tests were used to verify if there was a difference between the groups. Results: The most prevalent malocclusions after clinical examination were midline deviations, crowding and diastemas, and the ones that most scored in the adolescents' perception were crowding, misalignment and diastemas. Conclusion: The adolescents were able to perceive the aesthetic alterations resulting from malocclusions, being determinants of dissatisfaction when smiling. They were not ashamed to smile, did not consider that the ideal smile would improve their self-esteem and that misaligned teeth would not interfere with flirting and interpersonal relationships

Mercury biomagnification and the trophic structure of the ichthyofauna from a remote lake in the Brazilian Amazon.

The present study assesses mercury biomagnification and the trophic structure of the ichthyofauna from the Puruzinho Lake, Brazilian Amazon. In addition to mercury determination, the investigation comprised the calculation of Trophic Magnification Factor (TMF) and Trophic Magnification Slope (TMS), through the measurements of stable isotopes of carbon (δ13C) and nitrogen (δ15N) in fish samples. These assessments were executed in two different scenarios, i.e., considering (1) all fish species or (2) only the resident fish (excluding the migratory species). Bottom litter, superficial sediment and seston were the sources used for generating the trophic position (TP) data used in the calculation of the TMF. Samples from 84 fish were analysed, comprising 13 species, which were categorized into four trophic guilds: iliophagous, planktivorous, omnivorous and piscivorous fish. The δ13C values pointed to the separation of the ichthyofauna into two groups. One group comprised iliophagous and planktivorous species, which are linked to the food chains of phytoplankton and detritus. The other group was composed by omnivorous and piscivorous fish, which are associated to the trophic webs of phytoplankton, bottom litter, detritus, periphyton, as well as to food chains of igapó (blackwater-flooded Amazonian forests). The TP values suggest that the ichthyofauna from the Puruzinho Lake is part of a short food web, with three well-characterized trophic levels. Mercury concentrations and δ13C values point to multiple sources for Hg input and transfer. The similarity in Hg levels and TP values between piscivorous and planktivorous fish suggests a comparable efficiency for the transfer of this metal through pelagic and littoral food chains. Regarding the two abovementioned scenarios, i.e., considering (1) the entire ichthyofauna and (2) only the resident species, the TMF values were 5.25 and 4.49, as well as the TMS values were 0.21 and 0.19, respectively. These findings confirm that Hg biomagnifies through the food web of Puruzinho Lake ichthyofauna. The migratory species did not significantly change mercury biomagnification rate in Puruzinho Lake; however, they may play a relevant role in Hg transport. The biomagnification rate (TMS value) in Puruzinho Lake was higher than the average values for its latitude, being comparable to TMS values of temperate and polar systems (marine and freshwater environments).

Avaliação da Dureza de Materiais Restauradores Diretos Submetidos a Diferentes Desafios Erosivos / Hardness Evaluation of Direct Restorative Materials Submitted to Different Erosive Challenges

Objetivo: Avaliar a dureza de materiais restauradores diretos submetidos a diferentes desafios erosivos in vitro. Material e método: Trata-se de um estudo experimental realizado com 12 amostras de cada material restaurador medindo 4 mm de diâmetro x 2 mm de espessura. As amostras de resinas (microparticulada e nanoparticulada) e dos cimentos de ionômero de vidro (convencional e modificado por resina) foram confeccionadas e a dureza de superfície foi determinada antes e após uma semana de desafio erosivo, sendo a troca dos meios realizada a cada 24 horas. A análise Estatística dos dados foi feita por ANOVA de 2 fatores. O Teste t pareado avaliou a comparação entre as durezas iniciais e finais. Os testes estatísticos foram realizados pelo Programa Minitab (versão 17) com o nível de significância fixado em 5%. Resultados e Conclusão: Os resultados mostram que cimento de ionômero de vidro convencional e resina nanoparticulada apresentaram maiores valores de perda de dureza. Quantos aos meios, a Coca-Cola ocasionou maior perda de dureza no Cimento de ionômero de vidro convencional e o Powerade provocou maior grau de erosão no Cimento de ionômero de vidro modificado por resina. Todos os materiais testados apresentaram redução na dureza da superfície como resultado do desafio erosivo em bebidas ácidas, sendo o cimento de ionômero de vidro convencional o material mais afetado.

Objective: To evaluate in vitro the hardness of restorative materials submitted to different erosion challenges. Material and method: This was an experimental study using 12 samples of each restorative material measuring 4 mm diameter x 2 mm thick. Resins samples (microparticulate and nanoparticulate) and glass ionomer cements (conventional and resin-modified) were prepared and the surface hardness was determined before and after a week of erosive challenge, with the media being changed at each 24 hours. The statistical analysis was performed by two-way-ANOVA. The paired t test evaluated the comparison between the initial and final hardness. Statistical tests were performed with Minitab Program (version 17) and the significance level was set at 5%. Results and Conclusion: Conventional glass ionomer cement and nanoparticulate resin had higher hardness loss values. Regarding the beverages, Coca-Cola caused higher hardness loss in the conventional glass ionomer cement and Powerade lead to a higher degree of erosion in the glass ionomer cement modified by resin. Conclusion: All materials tested showed reduction in surface hardness as a result of erosive challenge in acidic drinks. Conventional glass ionomer cement was the most affected material.

Mercury distribution in target organs and biochemical responses after subchronic and trophic exposure to neotropical fish Hoplias malabaricus.

In the present study, we investigated the mercury distribution, mercury bioaccumulation, and oxidative parameters in the Neotropical fish Hoplias malabaricus after trophic exposure. Forty-three individuals were distributed into three groups (two exposed and one control) and trophically exposed to fourteen doses of methylmercury each 5 days, totalizing the doses of 1.05 µg g⁻¹ (M1.05) and 10.5 µg g⁻¹ (M10.5 group). Autometallography technique revealed the presence of mercury in the intestinal epithelia, hepatocytes, and renal tubule cells. Mercury distribution was dose-dependent in the three organs: intestine, liver, and kidney. Reduced glutathione concentration, glutathione peroxidase, catalase, and glutathione S-transferase significantly decreased in the liver of M1.05, but glutathione reductase increased and lipid peroxidation levels were not altered. In the M10.5, most biomarkers were not altered; only catalase activity decreased. Hepatic and muscle mercury bioaccumulation was dose-dependent, but was not influenced by fish sex. The mercury localization and bioaccumulation corroborates some histopathological findings in this fish species (previously verified by Mela et al. in Ecotoxicol Environ Saf 68:426-435, 2007). However, the results of redox biomarkers did not explain histopathological findings previously reported in M10.5. Thus, fish accommodation to the stressor may reestablish antioxidant status at the highest dose, but not avoid cell injury.

Flood pulse and spatial dynamics of mercury in sediments in Puruzinho lake, Brazilian Amazon / O pulso de inundação e a dinâmica espacial do mercúrio em sedimentos no lago Puruzinho, Amazônia Brasileira

The study was conducted in Puruzinho lake (Humaitل, AM) considering seasonal periods of rainy and dry in way to elucidate the flood pulse importance in the deposition, remobilization and distributions of mercury and organic matter in bottom sediments in the Madeira River Basin (Brazilian Amazon). Bottom sediments and soils samples were analyzed for total mercury and organic matter. Mercury concentrations obtained in bottom sediment were 32.20-146.40 ng g-1 and organic matter values were 3.5 - 18.0%. The main region for accumulation of mercury and organic matter was in the central and deepest lake area In the rainy season there was a greater distribution of Hg and organic matter, mainly controlled by means of income of the Madeira river water during flooding, while the predominant process in the dry season was the remobilization of total Hg due to the resuspension of bottom sediments.

Este estudo foi realizado no lago Puruzinho (Humaitá, AM) considerando os períodos de cheia e seca para investigar a importância do pulso de inundação na deposição, remobilização e distribuição das concentrações de mercúrio e matéria orgânica nos sedimentos de fundo no lago da bacia do rio Madeira, na Amazônia brasileira. As amostras de sedimentos e solos foram analisadas para mercúrio total e matéria orgânica. As concentrações mercúrio no sedimento de fundo variaram entre 32,20 e 146,40 ng g-1 e as porcentagens de matéria orgânica variaram de 3,5 a 18,0%. A principal região de acumulação de mercúrio e de matéria orgânica foi na área central e mais profunda do lago. Na estação chuvosa ocorreu maior distribuição de Hg e de matéria orgânica controlada principalmente pelo pulso de inundação do rio Madeira, enquanto que no período de seca predominam os processos de remobilização de mercúrio devido a resssuspensão do sedimento de fundo.

Total and methyl-mercury in hair and milk of mothers living in the city of Porto Velho and in villages along the Rio Madeira, Amazon, Brazil.

High fish consumption and extended breastfeeding are hallmarks of traditional lifestyle in Western Amazonia. We studied sources of mercury exposure, fish-methylmercury (meHg) and dental-amalgam filling, in 75 urban and 82 rural lactating mothers. Total mercury was determined in all samples while meHg concentrations were determined in subsamples of milk (45) and hair (27) of urban mothers living in Porto Velho (PV) and in hair (46) and milk (37) of mothers living in traditional communities (TC) of the Rio Madeira. The TC mothers showed significantly higher median hair-Hg concentrations (8.2µgg(-1)) than PV mothers (1.3µgg(-1)). Median total Hg in milk of PV mothers (0.36ngg(-1)) was significantly lower (p=0.0000) than that found in milk of TC mothers (2.30ngg(-1)). The median meHg concentrations in milk of TC mothers were also significantly higher (1.0ngg(-1)) than in milk of PV mothers (0.07ngg(-1)). For urban mothers with low fish consumption rates (and relatively higher dental amalgam fillings) the proportion of inorganic Hg in milk was higher (85%) than in TC (62%). In TC mothers 51% of breast milk Hg concentrations were above 2ngg(-1) (world median concentrations), contrasting with 8% in PV mothers. Despite this, there was no significant correlation between total Hg concentrations in milk and either amalgam filling or daily fish consumption. Socioeconomic changes occurring in Western Amazonia are causing changes in fish-eating habits of urban mothers. However, traditional lifestyle and attendant high fish consumption in riverine populations can still impact total Hg and methylmercury concentrations in mothers' milk and hair.

Mercury-selenium relationships in liver of Guiana dolphin: the possible role of Kupffer cells in the detoxification process by tiemannite formation.

Top marine predators present high mercury concentrations in their tissues as consequence of biomagnification of the most toxic form of this metal, methylmercury (MeHg). The present study concerns mercury accumulation by Guiana dolphins (Sotalia guianensis), highlighting the selenium-mediated methylmercury detoxification process. Liver samples from 19 dolphins incidentally captured within Guanabara Bay (Rio de Janeiro State, Brazil) from 1994 to 2006 were analyzed for total mercury (THg), methylmercury (MeHg), total organic mercury (TOrgHg) and selenium (Se). X-ray microanalyses were also performed. The specimens, including from fetuses to 30-year-old dolphins, comprising 8 females and 11 males, presented high THg (0.53-132 µg/g wet wt.) and Se concentrations (0.17-74.8 µg/g wet wt.). Correlations between THg, MeHg, TOrgHg and Se were verified with age (p<0.05), as well as a high and positive correlation was observed between molar concentrations of Hg and Se (p<0.05). Negative correlations were observed between THg and the percentage of MeHg contribution to THg (p<0.05), which represents a consequence of the selenium-mediated methylmercury detoxification process. Accumulation of Se-Hg amorphous crystals in Kupffer Cells was demonstrated through ultra-structural analysis, which shows that Guiana dolphin is capable of carrying out the demethylation process via mercury selenide formation.

Thermal comfort and personal protective equipment (PPE).

Personal Protective Equipment (PPE) is considered the last preferred solution to an Occupational Safety Health (OSH) system. Brazilian OSH legislation assumes and requires PPE usage to neutralize the possibility of pesticide contamination. This study examines the inadequacy of the PPE used in a tomato crop, particularly from the standpoint of thermal comfort. This study made observations regarding heart rate and body temperature in a controlled environment using a treadmill (with and without PPE) of 12 volunteers; and during the process of rural labor (at rest and in normal work routine) of 2 actual rural workers. Comparing the results of the treadmill test (with and without PPE) which tried to reproduce the same level of effort and environmental conditions of the actual rural work, this study demonstrated that PPE makes thermoregulation harder and tympanic temperature reaches higher values with the same level of effort. Moreover, body temperature increases quickly: with PPE it took 15 minutes to raise 1°C in body temperature, compared to 40 minutes without PPE. The results of this study indicated that the use inadequate PPE might pose thermoregulation risk to rural workers.

Gluteoplastia de aumento: a importância do ensino na formação atual do residente frente à demanda crescente / Buttocks increase: the importance of teaching in current resident facing increased demand

INTRODUÇÃO: Frente ao aumento na demanda por gluteoplastia de aumento com implantes nos últimos anos, é importante que haja profissionais treinados e capacitados para realizar tal procedimento e suas associações com lipoaspiração/lipoenxertia, com o intuito final de melhorar o contorno do quadril. MÉTODO: Ao ver na residência médica o local de ensino apropriado para tal prática, elaboramos o estudo de 20 casos tratados por médicos residentes em treinamento sob supervisão, a fim de observar a aplicabilidade e exequibilidade do método e seus resultados. RESULTADOS: Alcançamos melhoria do contorno glúteo em todos pacientes, o que foi corroborado pelo nível de satisfação elevado de 100 por cento delas e pequena taxa de complicações, compatíveis com a literatura. Há certo receio no início do aprendizado, devido ao descolamento intramuscular às cegas, mas guiados por parâmetros técnicos e anatômicos precisos e sob supervisão qualificada, conseguiu-se a realização da cirurgia no plano intramuscular adequado, associando a lipoaspiração e lipoenxertia em alguns casos, o que proporcionou ótima cobertura do implante e resultados naturais no contorno final do quadril. CONCLUSÃO: O estudo demonstrou a grande aplicabilidade do ensino da gluteoplastia de aumento com implantes ao residente em formação, visto a necessidade de uma formação adequada, completa e segura, para atender à crescente demanda por implantes glúteos observada no mercado atual.

INTRODUCTION: Facing the increasing demand for buttocks augmentation with implants in recent years, it is important that there are enough trained and qualified to perform such a procedure and their associations with liposuction/fat grafting with an ultimate aim of improving the contour of the hip. METHODS: Seeing in the residency teaching sites suitable for such a practice, we make a study of 20 cases treated by resident physicians in training under supervision in order to observe the applicability and feasibility of the method and its results. RESULTS: Achieved the improvement of the gluteal contour in all patients, which was corroborated by the high level of satisfaction 100 percent of them and low rate of complications, consistent with the literature. It is known that there is a certain fear in the beginning of learning, due to detachment in blind, but guided by precise anatomical and technical parameters and under qualified supervision, we had the surgery in the plan adequate intramuscular associated with liposuction and fat grafting in some cases, which provided excellent coverage of the implant and natural results in the final contour of the hip. CONCLUSION: The study showed the wide applicability of the teaching of buttocks augmentation with implants, to the resident in training, given the need for adequate training to meet the growing demand in the market today.

Mercury and DDT exposure risk to fish-eating human populations in Amazon.

In order to investigate the potential risk of mercury and DDTs exposure to fish-eating human populations in Samuel Reservoir, not affected directly by gold-mining activities, the axial muscle of Cichla monoculus was analyzed. Twenty-nine and thirty adults individuals were collected respectively on February (rainy season) and August (dry season) 2007. The specimens were sacrificed by spinal section before sex identification, body weight and total length determination. For total mercury, DDT and DDE quantifications and cholinesterase activity samples of the axial muscle were frozen at -20°C, and for histopathological studies gill and liver were fixed in ALFAC solution for 16 h. A value of 48.2% and 33% of the individuals, respectively from rainy and dry seasons, presented mercury concentrations higher than the maximum established for safe human consumption (0.5 µg g⁻¹) by World Health Organization. A positive correlation between body weight and Hg concentration was observed only in individuals from the rainy season, but no correlation was observed to DDT and DDE from both seasons. Differently from that observed to mercury, DDT levels presented a significant difference between both studied seasons, but no correlation was observed for both mercury and DDTs and sex. The levels of DDTs in muscle of C. monoculus are under the maximum established by FAO-Alimentarius CODEX and Swedish Food Regulation for human consumption. The histopathological and neurotoxic findings showed that the wild population of fish is affected by chronic exposure to mercury, meaning risk also to fish-eating populations. Finally, the results showed that C. monoculus is an important vehicle for human exposure to mercury and DDTs in Samuel Reservoir and that it is necessary a continuous biomonitoring of the levels of both pollutants in order to manage the risk of exposure to human populations.

Fish consumption by traditional subsistence villagers of the Rio Madeira (Amazon): impact on hair mercury.

BACKGROUND: Subsistence ribeirinhos of the Amazon Basin depend on fish for their principal source of protein, but fish availability changes with seasonal high and low waters. AIM: To assess taxa and quantity of fish consumed and estimate attendant exposure to methyl-Hg in a traditional subsistence high fish-eating community of the Amazon Basin. SUBJECTS AND METHODS: 120 villagers in 18 households were followed for 6 months (August to February) for weighed portions of fish consumed. RESULTS: Mean daily per capita fish consumption was high (406 g/day) with fish meals ranging from 4 to 14 times/week and an integrated yearly consumption of 148.2 kg/person. Median total-Hg concentrations in fish ranged from 0.011 to 0.409 ppm; six of the more consumed fish species comprised more than 50% of the fish consumed. The villagers mean hair-Hg concentration was high (17.4 +/- 11.5 microg/g), with both inter- and intra-household variation despite similar high fish consumption; only 7% showed hair-Hg concentrations < 5 microg/g, but 75% had hair-Hg levels above 10 microg/g. Maternal hair-Hg was significantly correlated with respective children's hair-Hg (Spearman r = 0.5390; p < 0.0001). The high daily fish intake of these villagers is predominantly of species with much lower Hg concentrations. CONCLUSION: In Amazonian lifestyle of ribeirinho communities traditional fish consumption is high and depends on available species; fish is also the principal via of meHg exposure and attendant hair-Hg concentrations.