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1.
Biomark Med ; 9(5): 443-52, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25275858

RESUMEN

AIM: Determine reference ranges of lymphocyte subsets in healthy Qatari adults. PATIENTS & METHODS: Blood samples (n = 150) were investigated using four colors flow cytometery. RESULTS: The mean percentage and absolute values of the lymphocyte subsets were: CD3: 73.6 ± 6.1%; 2,072 ± 644 cells/µl; CD4: 41.1 ± 7.9%; 1,167 ± 396 cells/µl; CD8: 33.8 ± 7.1%; 967 ± 364 cells/µl; B cells (CD19(+)): 11.3 ± 3.9%; 318 ± 144 cells/µl and natural killer (NK) cells (CD16/56(+)): 16.5 ± 6.3%, 440 ± 401 cells/µl, respectively. The CD4/CD8 ratio was 1.3 ± 0.5. The ratio, CD3(+) and CD4(+) percentage and CD4(+) cell counts were significantly higher in females; CD8(+) and NK cell count and CD8(+) percentage were significantly higher in males. The impact of age was less certain. CONCLUSION: The reference values of lymphocyte subsets appear to be specific to this population, demonstrating the importance of establishing local reference ranges.


Asunto(s)
Subgrupos Linfocitarios/citología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Relación CD4-CD8 , Femenino , Citometría de Flujo , Voluntarios Sanos , Humanos , Subgrupos Linfocitarios/metabolismo , Masculino , Persona de Mediana Edad , Valores de Referencia , Adulto Joven
2.
Int J Gen Med ; 6: 13-8, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23378780

RESUMEN

OBJECTIVE: To investigate the diagnostic utility of interferon-gamma (IFN-γ) and adenosine deaminase (ADA) in tuberculous pleural effusions by determining the best cutoff levels of these two markers for pleural tuberculosis, in the context of the local epidemiological settings in Qatar. METHODS: We prospectively studied IFN-γ and ADA levels in the pleural fluid of patients presenting to Hamad General Hospital between June 1, 2009 and May 31, 2010. RESULTS: We studied 103 patients with pleural effusions, 72 (69.9%) with pleural tuberculosis, and 31 (30.1%) with nontuberculous etiologies. The mean IFN-γ concentration for the group with tuberculous effusions was significantly higher than that in the group with nontuberculous effusions (1.98 ± 81 vs 0.26 ± 10 pg/mL [P < 0.0001]). The mean ADA activity for the tuberculous effusions group was significantly higher than that in group with nontuberculous effusions (41.30 ± 20.09 vs 14.93 ± 14.87 U/L [P < 0.0001]). By analysis of receiver operating characteristic (ROC) curves, the best cutoff values for IFN-γ and ADA were 0.5 pg/mL and 16.65 U/L, respectively. The results for IFN-γ vs ADA were: for sensitivity, 100% vs 86%, respectively; for specificity, 100% vs 74%, respectively; for positive predictive value, 100% vs 88.5%, respectively; and for negative predictive value, 100% vs 69.7%, respectively. CONCLUSION: IFN-γ and ADA could be used as valuable parameters for the differentiation of tuberculous from nontuberculous effusion, and IFN-γ was more sensitive and specific for tuberculous effusion than ADA.

3.
Med Princ Pract ; 15(5): 368-72, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16888395

RESUMEN

OBJECTIVES: The aim of this study was to determine the prevalence of hepatitis B surface antigen (HBsAg), hepatitis B core antibodies (anti-HBc) and hepatitis B virus (HBV) DNA among a selected group of Omani blood donors. MATERIALS AND METHODS: Two hundred HBsAg-negative donors were screened for anti-HBc. Those found to be positive were investigated for HBV DNA by polymerase chain reaction. HBsAg was retested on these sera following an immune complex dissociation technique. RESULTS: HBsAg was present in 2.8% of the donors. Forty-one out of 200 (20.5%) HBsAg-negative donors were positive for anti-HBc. Eleven were positive for HBsAg after dissociation, whereas 8 gave readings just over the cutoff. HBV DNA was not detected in this group. CONCLUSION: Findings indicate that testing donors for HBsAg alone is not sufficient to eliminate HBV from the blood supply in Oman.


Asunto(s)
Donantes de Sangre , Hepatitis B/epidemiología , ADN Viral/análisis , Hepatitis B/sangre , Anticuerpos contra la Hepatitis B/análisis , Antígenos de Superficie de la Hepatitis B/análisis , Humanos , Omán/epidemiología , Reacción en Cadena de la Polimerasa , Prevalencia
4.
Sultan Qaboos Univ Med J ; 6(2): 27-31, 2006 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-21748132

RESUMEN

OBJECTIVE: To ascertain the relationship between serum leptin levels and related variables (weight, Body Mass Index (BMI) and fat percentage) in a group of Omani obese and non-obese healthy subjects. METHODS: Leptin levels were assessed in serum samples from 35 obese Omanis and 20 non-obese healthy subjects. RESULTS: There was a significant difference (p< 0.001) in serum leptin between the obese group (34.78 + 13.96 ng/ml) and the control non-obese subjects (10.6 ± 4.2 ng/ml). Leptin levels were higher in females compared to males. There was a significantly positive correlation between leptin levels in obese subjects with weight (p=0.002), body fat percentage (p=0.0001) and BMI (p=0.001). CONCLUSIONS: We concluded that serum leptin levels are higher in the Omani obese group and correlate positively with body fatness and obesity.

5.
Int J Food Microbiol ; 103(3): 347-51, 2005 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-16099316

RESUMEN

The aim of the study was to evaluate the antimicrobial effect and the ability of honey to prevent Salmonella interitidis adhering to intestinal epithelial cells in vitro. Antimicrobial activity was demonstrated. Bacterial adherence was assayed using S. interitidis cells that had been incubated first with honey and then with the intestinal epithelial cells. Results showed that honey at dilutions of up to 1:8 reduced bacterial adherence from 25.6+/-6.5 (control) to 6.7+/-3.3 bacteria per epithelial cell (P<0.001).


Asunto(s)
Adhesión Bacteriana/efectos de los fármacos , Células Epiteliales/microbiología , Miel , Infecciones por Salmonella/prevención & control , Salmonella/fisiología , Células Cultivadas , Recuento de Colonia Microbiana , Miel/análisis , Humanos , Salmonella/crecimiento & desarrollo
6.
Med Princ Pract ; 14(4): 209-12, 2005.
Artículo en Inglés | MEDLINE | ID: mdl-15961927

RESUMEN

OBJECTIVE: To determine the levels of anti-thyroid-stimulating hormone receptor antibody (TRAb) and thyroid peroxidase antibodies (TPO) in Omani patients with Graves' disease (GD). SUBJECTS AND METHODS: ELISA was used to measure the levels of TRAb and TPO in sera of 111 patients with GD, 40 patients with systemic lupus erythematosus (SLE) and 50 healthy normal subjects. Seventy-eight GD patients received iodine-131 treatment while 33 newly diagnosed patients did not receive any treatment. RESULTS: The levels of TRAb in the patients with GD, SLE and normal subjects were 7.24 9+/-0.623, 1.04+/-0.179 and 0.539+/-0.070 units/liter, respectively. Elevated levels of TRAb were found in 75, 5 and 2% of patients with GD, SLE and normal subjects, respectively. For anti-TPO antibodies, the levels were 713.331+/-97.905, 85.575+/-34.936 and 22.96+/-4.544 lU/ml, for patients with GD, SLE and normal subjects, respectively. The elevated level of anti-TPO antibodies were found in 72, 15 and 6% of patients with GD, SLE and normal subjects. Statistically significant differences in positive levels of TRAb and anti-TPO antibodies were found in patients with GD as compared to SLE and normal subjects (p<0.001). The treated GD patients had lower levels of TRAb than untreated, but no difference was detected in the levels of anti-TPO antibodies between the treated and not treated. CONCLUSION: High levels of TRAb and TPO were detected in Omani patients with GD. TRAb may be helpful in the diagnosis and monitoring the efficacy of iodine-131 treatment in GD patients.


Asunto(s)
Autoanticuerpos/inmunología , Enfermedad de Graves/diagnóstico , Yoduro Peroxidasa/inmunología , Receptores de Tirotropina/inmunología , Adolescente , Adulto , Anciano , Autoanticuerpos/sangre , Biomarcadores/sangre , Diagnóstico Diferencial , Ensayo de Inmunoadsorción Enzimática , Femenino , Enfermedad de Graves/sangre , Humanos , Yoduro Peroxidasa/sangre , Lupus Eritematoso Sistémico/sangre , Lupus Eritematoso Sistémico/inmunología , Masculino , Persona de Mediana Edad , Omán , Receptores de Tirotropina/sangre , Pruebas de Función de la Tiroides
7.
Am J Hematol ; 77(4): 323-8, 2004 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-15551290

RESUMEN

The aim of our study was to assess the cytokine profile of sickle cell disease (SCD) patients in steady state and in vaso-occlusive crisis (VOC). VOC has a complex nature, involving interactions between sickle red blood cells (RBC), the endothelium, and leucocytes. Endothelial damage due to recurrent adhesion of sickle RBCs may disrupt endothelial function, leading to altered cytokine release. It is therefore pertinent to study the cytokine profile of SCD patients in steady state and in crisis prior to exploring its contribution to vaso-occlusive manifestations, since it is believed that an altered balance of proinflammatory and anti-inflammatory cytokines plays an important role in painful crisis. Cytokines including IL-1beta, IL-2, IL-4, IL-6, IL-8, TNF-alpha, and IFN-gamma were measured by commercially available ELISA kits in SCD patients (n = 60); in steady state (n = 26) and in painful crisis (n = 34) and compared with nonanemic age- and sex-matched normal Omani controls (n = 20). SCD patients in crisis showed elevated levels of TNF-alpha (P < 0.092) and IL-6 (P < 0.024) when compared with steady state. It was also observed that SCD patients in steady state showed a significant elevation in IL-1beta (P < 0.04), IL-6 (P < 0.0001), and IFN-gamma (P < 0.02) as compared to normal subjects. It is thus evident that both type I and type II cytokines are significantly altered in SCD patients. In steady state, type II proinflammatory cytokines are elevated, whereas in crisis, an additional augmentation of type I cytokines occurs, with persistent elevation of type II cytokines, emphasizing the role of perturbed endothelium and activated monocytes in the pathophysiology of vaso-occlusion in sickle cell crisis.


Asunto(s)
Anemia de Células Falciformes/sangre , Citocinas/sangre , Enfermedades Vasculares/sangre , Adulto , Anemia de Células Falciformes/complicaciones , Proteína C-Reactiva/metabolismo , Creatinina/sangre , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Pruebas de Función Hepática , Masculino , Omán , Enfermedades Vasculares/etiología
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