RESUMEN
Mutations in STXBP1 have recently been identified as a cause of infantile epileptic encephalopathy. The underlying mechanism of the disorder remains unclear and, recently, several case reports have described broad and progressive neurological phenotypes in addition to early-onset epilepsy. Herein, we describe a patient with early-onset epilepsy who subsequently developed a progressive neurological phenotype including parkinsonism in her early teens. A de novo mutation in STXBP1 (c.416C>T, p.(Pro139Leu)) was detected with exome sequencing together with profound impairment of complex I of the mitochondrial respiratory chain on muscle biopsy. These findings implicate a secondary impairment of mitochondrial function in the progressive nature of the disease phenotype.
Asunto(s)
Complejo I de Transporte de Electrón/deficiencia , Epilepsia/genética , Enfermedades Mitocondriales/genética , Proteínas Munc18/genética , Mutación Missense , Trastornos Parkinsonianos/genética , Encéfalo/fisiopatología , Niño , Progresión de la Enfermedad , Electroencefalografía , Complejo I de Transporte de Electrón/genética , Epilepsia/complicaciones , Exoma , Femenino , Humanos , Enfermedades Mitocondriales/complicaciones , Trastornos Parkinsonianos/complicaciones , FenotipoRESUMEN
This study evaluated the effect of eye muscle area (EMA), ossification, carcass weight, marbling and rib fat depth on the incidence of dark cutting (pH(u)>5.7) using routinely collected Meat Standards Australia (MSA) data. Data was obtained from 204,072 carcasses at a Western Australian processor between 2002 and 2008. Binomial data of pH(u) compliance was analysed using a logit model in a Bayesian framework. Increasing eye muscle area from 40 to 80 cm², increased pH(u) compliance by around 14% (P<0.001) in carcasses less than 350 kg. As carcass weight increased from 150 kg to 220 kg, compliance increased by 13% (P<0.001) and younger cattle with lower ossification were also 7% more compliant (P<0.001). As rib fat depth increased from 0 to 20mm, pH(u) compliance increased by around 10% (P<0.001) yet marbling had no effect on dark cutting. Increasing musculature and growth combined with good nutrition will minimise dark cutting beef in Australia.
Asunto(s)
Bovinos/crecimiento & desarrollo , Calidad de los Alimentos , Glucógeno/metabolismo , Carne/normas , Músculo Esquelético/crecimiento & desarrollo , Desarrollo Musculoesquelético , Columna Vertebral/crecimiento & desarrollo , Adiposidad , Animales , Australia , Composición Corporal , Peso Corporal , Calcificación Fisiológica , Grasas de la Dieta/análisis , Femenino , Inspección de Alimentos/legislación & jurisprudencia , Concentración de Iones de Hidrógeno , Legislación Alimentaria , Masculino , Carne/análisis , Modelos Biológicos , Músculo Esquelético/metabolismo , Grasa Subcutánea Abdominal/crecimiento & desarrollo , Australia OccidentalAsunto(s)
Proteínas de Ciclo Celular/genética , ADN Mitocondrial/genética , Eliminación de Gen , Predisposición Genética a la Enfermedad/genética , Mutación/genética , Oftalmoplejía Externa Progresiva Crónica/genética , Ribonucleótido Reductasas/deficiencia , Ribonucleótido Reductasas/genética , Adulto , Factores de Edad , Estudios de Cohortes , Femenino , Humanos , Masculino , Enfermedades Mitocondriales/diagnóstico , Enfermedades Mitocondriales/genética , Enfermedades Mitocondriales/metabolismo , Oftalmoplejía Externa Progresiva Crónica/diagnóstico , Oftalmoplejía Externa Progresiva Crónica/metabolismoAsunto(s)
ADN Mitocondrial/genética , ADN Mitocondrial/orina , Pruebas Genéticas/métodos , Enfermedades Mitocondriales/diagnóstico , Enfermedades Mitocondriales/genética , Evaluación de Resultado en la Atención de Salud/métodos , Precursores del ARN/genética , ARN de Transferencia de Leucina/genética , Adulto , Anciano , Femenino , Predisposición Genética a la Enfermedad/genética , Humanos , Masculino , Persona de Mediana Edad , Enfermedades Mitocondriales/orina , Mutación , Pronóstico , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
Information on farm work-related injuries was sought to assist in the design of effective farm safety prevention programs. A telephone survey was conducted using a stratified random sample of 919 sheep/wool, beef cattle and dryland broadacre cropping farms from three shires in the wheat/sheep belt of New South Wales. The adjusted response rate was 84%. There were 425 reported injuries over an 18-month period. One in five farms reported at least one injury per year, while one in 12 farms reported at least one serious injury per year. Animal-related injuries were the largest major category for agent of injury, while the largest category for nature of injury was sprain and strain, recording almost one-quarter of all injuries. The farm workshop or shed was the most common location of injury, with more than 20% of all reported injuries occurring there. Personal risk factors thought to contribute to these farm work-related injuries were examined. The statistically significant personal risk factors for injury occurrence were age (and/or experience), previous injury status, body mass index, hours of sleep, a variable measuring daytime drowsiness and a variable measuring perceived stress.