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4.
Clin Exp Immunol ; 121(2): 375-83, 2000 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10931156

RESUMEN

Adhesion molecules regulate the migration of lymphocytes in lymphoid and non-lymphoid organs. In the lung, little is known about lymphocyte sticking and migration through the pulmonary vascular endothelium in physiological or pathological situations. Therefore the isolated buffer-perfused rat lung was used to investigate the mobilization of lymphocytes out of the normal lung into the venous effluent and to the bronchoalveolar space. The lymphocyte subset composition was characterized in the venous effluent, the lung tissue and the bronchoalveolar lavage (BAL) using immunocytology. Lymphocytes continuously left the normal lung at a total of 5.0 +/- 0.7 x 106 cells within the first hour of perfusion. The injection of 200 x 106 lymphocytes via the pulmonary trunk increased the venous release of lymphocytes by 170%. To investigate the effect of LFA-1 and CD44 on the adhesion of lymphocytes to the pulmonary endothelium, lymphocytes preincubated with an anti-LFA-1 MoAb, which blocks the interaction of LFA-1 and intercellular adhesion molecule-1 (ICAM-1), or lymphocytes preincubated with an anti-CD44 MoAb, were injected. The injection of LFA-1-blocked lymphocytes led to an increase by 70% of injected cells recovered in the perfusate within the first hour, whereas anti-CD44 treatment of injected lymphocytes had no effect. The LFA-1-blocked lymphocytes showed higher numbers of T and B cells in the effluent. Thus, the present experiments demonstrate that LFA-1 influences the trapping of lymphocytes in the vasculature of the healthy rat lung.


Asunto(s)
Anticuerpos Monoclonales/farmacología , Quimiotaxis de Leucocito/fisiología , Endotelio Vascular/metabolismo , Pulmón/irrigación sanguínea , Antígeno-1 Asociado a Función de Linfocito/metabolismo , Animales , Anticuerpos Monoclonales/inmunología , Líquido del Lavado Bronquioalveolar/citología , Adhesión Celular , Femenino , Receptores de Hialuranos/inmunología , Receptores de Hialuranos/fisiología , Molécula 1 de Adhesión Intercelular/metabolismo , Recuento de Linfocitos , Antígeno-1 Asociado a Función de Linfocito/inmunología , Masculino , Perfusión , Unión Proteica/efectos de los fármacos , Venas Pulmonares/citología , Ratas , Ratas Endogámicas Lew
6.
J Pharmacol Exp Ther ; 286(3): 1309-14, 1998 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-9732393

RESUMEN

Prostanoids have been implicated in the regulation of lung vascular tone both under physiological and inflammatory conditions. The conversion of arachidonic acid (AA) to prostaglandin H2 is catalyzed at least by two isoforms of cyclooxygenase, named Cox-1 and Cox-2. Cox-1 is thought to be ubiquitously expressed, enrolled in physiological processes, whereas Cox-2 is mostly assumed to be dynamically regulated, responding to inflammatory conditions. We have recently shown by immunohistochemistry that Cox-2 is constitutively expressed in control rat lungs, with a predominant localization in smooth muscle cells of partially muscular vessels. We now asked whether Cox-2 is basically involved in the physiological regulation of pulmonary vascular tone. Isolated perfused rat lungs were challenged with intravascular bolus application of free AA to elicit thromboxane-related vasoconstrictor responses and to investigate the effects of three different selective Cox-2 inhibitors (NS-398, DUP697, SC-236). AA induced the liberation of prostaglandin I2 and thromboxane A2 into the intravascular space, and it provoked marked pulmonary artery pressure responses and concomitant lung edema formation. All events were dose-dependently inhibited by 1 to 50 micromol/liter NS-398, whereas control vasoconstrictor responses to angiotensin II and the stable thromboxane analogue U46619 were not affected by this agent. Similarly, marked inhibition of the AA elicited pressor response was achieved by 25 micromol/l DUP697 and by 10 micromol/l SC-236. These data suggest a physiological role of Cox-2 rather than Cox-1 in the regulation of vascular tone in rat lungs.


Asunto(s)
Isoenzimas/fisiología , Pulmón/irrigación sanguínea , Prostaglandina-Endoperóxido Sintasas/fisiología , Prostaglandinas/biosíntesis , Animales , Ácido Araquidónico/farmacología , Presión Sanguínea/efectos de los fármacos , Ciclooxigenasa 2 , Relación Dosis-Respuesta a Droga , Pulmón/efectos de los fármacos , Nitrobencenos/farmacología , Ratas , Ratas Sprague-Dawley , Sulfonamidas/farmacología , Aumento de Peso/efectos de los fármacos
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