Canine Tonsillar Polyps: Characteristics, Classification, and Review of the Pathogenesis.

Canine tonsillar polyps are uncommon. We describe 14 tonsillar polyps in dogs and review their classification and pathogenesis. All dogs were adult (3-13 years old). Females (10/14) were more affected than males (4/14). Most of the lesions were asymptomatic (10/14). All lesions were unilateral, pedunculated (9/14), or sessile (5/14), with a smooth (12/14) or papillary/verrucous surface (2/14). Histologically, polyps consisted of benign proliferation of lymphatic vessels, blood vessels, fibrous tissue, and lymphoid tissue in variable proportions, with occasional adipose tissue (4/14). According to the main stromal components, polyps were categorized as lymphangiomatous (5/14), lymphangiolipomatous (2/14), lymphangiofibromatous (2/14), angiofibromatous (1/14), angiofibrolipomatous (1/14), lymphoid (2/14), and myxomatous (1/14). As the pathogenesis of these polyps remains unclear, we propose to replace the term inflammatory tonsillar polyp by a morphological diagnosis based on the stromal characteristics of the lesions. Simple surgical excision was curative in the 9 cases with available follow-up information.

MicroRNA Expression in Formalin-Fixed, Paraffin-Embedded Samples of Canine Cutaneous and Oral Melanoma by RT-qPCR.

MicroRNAs (miRNAs) are a class of small, noncoding RNA that post-transcriptionally regulate protein expression. miRNAs are emerging as clinical biomarkers of many diseases, including tumors. The aim of this study was to investigate whether miRNA expression could vary in melanoma samples derived from formalin-fixed, paraffin-embedded (FFPE) tissues. The study included 4 groups: (1) 9 samples of oral canine malignant melanoma, (2) 10 samples of cutaneous malignant melanoma, (3) 5 samples of healthy oral mucosa, and (4) 7 samples of healthy skin. The expression levels of 6 miRNAs-miR-145, miR-146a, miR-425-5p, miR-223, miR-365, and miR-134-were detected and assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) using TaqMan probes. Cutaneous canine malignant melanoma showed a decrease of the expression level of miR-145 and miR-365 and an increase of miR-146a and miR-425-5p compared to control samples. MiR-145 was also downregulated in oral canine malignant melanoma. The miRNAs with decreased expression may regulate genes involved in RAS, Rap1, and transforming growth factor ß (TGF-ß) signaling pathways, as well as upregulated genes associated with phosphatidylinositol signaling system, adherens junction, and RAS signaling pathways. In conclusion, miR-145, miR-365, miR-146a, and miR-425-5p were differentially expressed in canine malignant melanoma and healthy FFPE samples, suggesting that they may play a role in canine malignant melanoma pathogenesis.

First description of Bartonella koehlerae infection in a Spanish dog with infective endocarditis.

BACKGROUND: Bartonella koehlerae has been recently described as a new cat- and cat fleas-associated agent of culture-negative human endocarditis. It has been also encountered in one dog from Israel and six dogs from the USA, but other clinically relevant reports involving this bacterium are lacking. RESULTS: A 7-year-old intact male mixed dog presented with clinico-pathological signs consistent with mitral endocarditis and cutaneous hemangiosarcoma. Molecular studies revealed the presence of Bartonella koehlerae DNA in samples from blood and mitral valve tissue. CONCLUSIONS: This is the first description of B. koehlerae in Spain, corroborating that it can also be detected in dogs. Bartonella koehlerae infection should also be considered in Spain in humans and dogs presenting with clinical disease suggestive of it, such as culture-negative endocarditis.

Mandibular odontogenic sarcoma (ameloblastic fibrodentinosarcoma) in an aged cat - Short communication.

A 13-year-old male cat presented with an ill-defined mass in the rostral mandible causing destruction and loss of alveolar bone. Microscopically, the mass consisted of cords or islands of benign odontogenic epithelium and a malignant, pleomorphic spindle-shaped cell component with dysplastic dentine formation. Immunohistochemically, neoplastic mesenchymal cells proved to be strongly positive for vimentin and negative for cytokeratins, desmin, actin and S100 protein; the Ki67 proliferation index was high. Morphological and immunohistochemical features largely overlap those reported for ameloblastic fibrodentinosarcoma, an uncommon histologic subtype of odontogenic sarcoma recognised in humans but no reported previously in animals. Ki-67 expression assessment may help to discriminate between malignant and benign forms of odontogenic tumours but the final diagnosis is mainly morphological.

Clinical, histopathological and epidemiological study of canine straelensiosis in the Iberian Peninsula (2003-2007).

Straelensiosis is a relatively new disease described in dogs and produced by a trombiculid larva known as Straelensia cynotis. Few cases have been reported in the English literature. Straelensiosis has been observed in Southern France and Northern Portugal causing a distinctive nodular dermatitis. The present study describes the clinical, histopathological and epidemiological features of 19 cases diagnosed as straelensiosis in the north of Spain and Portugal (2003-2007). A follow-up of the animals after diagnosis was carried out. Differences with previously described cases were observed, especially concerning epidemiology and histopathology. The grade of response to different treatments and new microscopic features are discussed. The study concludes that S. cynotis can cause a nodular dermatitis in rural and hunting dogs in Spain and Portugal and is associated with particular seasonal and geographical factors.

Clinical and histopathological features of a thymolipoma in a dog.

This study presents a case of a canine thymolipoma, which is a rare, slow-growing, benign tumor of the thymus composed of mature adipose tissue and thymic tissue. A 9-year-old spayed, female miniature pinscher presented with chronic cough and dyspnoea. Radiology revealed pleural effusion and a mediastinal mass with a fatty appearance. The mass was attached to, and silhouetted, the adjacent pericardium. Microscopically, the mass was composed of adipose tissue with numerous cords and nests of thymic tissue without corticomedullary arrangement. To the authors' knowledge, this is the first description of this uncommon neoplasm in a dog. The gross and histological findings are similar to those described for thymolipomas in humans. The possible histogenesis for this neoplasia in a dog is also discussed.