RESUMEN
La atención a la diversidad (AD) es un objetivo clave en la educación, también en la Formación Profesional Básica, que se caracteriza por ser un ámbito educativo de gran heterogeneidad del alumnado. Se presentan los resultados de un estudio ex-post facto realizado con objeto de analizar la sensibilidad del profesorado hacia la AD y los factores relacionados con la misma. La investigación se lleva a cabo mediante un diseño no experimental (ex-post facto) con una muestra representativa de 218 profesores de Formación Profesional Básica. Los resultados indican que el profesorado más sensibilizado con la AD concede mayor importancia al trabajo cooperativo, al rol desempeñado por el docente para fomentar el trabajo en equipo, a las tutorías, al compromiso de las familias, al apoyo de los compañeros y compañeras y a la coordinación y el funcionamiento del centro educativo. Se subraya también la importancia del trabajo en red para mejorar la formación del alumnado y la necesidad de profundizar en este tema para obtener, a nivel nacional, una visión holística e integrada de la situación de la Formación Profesional Básica, que contribuya a la mejora de la atención del alumnado en general y, especialmente, de quienes están en riesgo de exclusión educativa y social. (AU)
The objective of this research is to analyze the sensitivity of Basic VocationalTraining (BVT) teaching faculty towards attention to diversity (AD) and factors related to the same. This research presents the results of an ex post facto study carried out with a representative sample of 218 Basic Vocational Training teachers. The results show that the teaching faculty most aware of AD give greater importance to cooperative work, to the role played by the teacher, to tutorials, to the commitment of families, to the support of their team-mates and to the coordination and functioning of the school. The need to deepen the networking is underlined in order to obtain a holistic and integrated vision of the situation of BVT which contributes to the improvement of students attention in general and, especially, of those who are at risk of educational and social exclusion. (AU)
Asunto(s)
Humanos , Diversidad de Género , Capacitación Profesional , Prácticas Interdisciplinarias , EducaciónRESUMEN
The type VI secretion system (T6SS) of Pseudomonas aeruginosa injects effector proteins into neighbouring competitors and host cells, providing a fitness advantage that allows this opportunistic nosocomial pathogen to persist and prevail during the onset of infections. However, despite the high clinical relevance of P. aeruginosa, the identity and mode of action of most P. aeruginosa T6SS-dependent effectors remain to be discovered. Here, we report the molecular mechanism of Tse5-CT, the toxic auto-proteolytic product of the P. aeruginosa T6SS exported effector Tse5. Our results demonstrate that Tse5-CT is a pore-forming toxin that can transport ions across the membrane, causing membrane depolarisation and bacterial death. The membrane potential regulates a wide range of essential cellular functions; therefore, membrane depolarisation is an efficient strategy to compete with other microorganisms in polymicrobial environments.
Asunto(s)
Pseudomonas aeruginosa , Sistemas de Secreción Tipo VI , Pseudomonas aeruginosa/metabolismo , Potenciales de la Membrana , Proteínas Bacterianas/metabolismo , Sistemas de Secreción Tipo VI/genética , Sistemas de Secreción Tipo VI/metabolismoRESUMEN
Recent reports indicate that the Type six secretion system exported effector 8 (Tse8) is a cytoactive effector secreted by the Type VI secretion system (T6SS) of the human pathogen Pseudomonas aeruginosa. The T6SS is a nanomachine that assembles inside of the bacteria and injects effectors/toxins into target cells, providing a fitness advantage over competing bacteria and facilitating host colonisation. Here we present the first crystal structure of Tse8 revealing that it conserves the architecture of the catalytic triad Lys84-transSer162-Ser186 that characterises members of the Amidase Signature superfamily. Furthermore, using binding affinity experiments, we show that the interaction of phenylmethylsulfonyl fluoride (PMSF) to Tse8 is dependent on the putative catalytic residue Ser186, providing support for its nucleophilic reactivity. This work thus demonstrates that Tse8 belongs to the Amidase Signature (AS) superfamily. Furthermore, it highlights Tse8 similarity to two family members: the Stenotrophomonas maltophilia Peptide Amidase and the Glutamyl-tRNAGln amidotransferase subunit A from Staphylococcus aureus.
