Endometrial fluid biomarkers and their potential as predictors of successful embryo implantation.

Background: Embryo implantation is a complex biological process which requires synchronized dialogue between the receptive endometrium and the blastocyst. The endometrium, however, is only receptive to embryo implantation for a very short period. Recurrent implantation failure (RIF) is a major challenge in assisted reproductive techniques mainly due to impaired receptivity, but there is still a need for a reliable and valid clinical test to assess endometrial receptiveness, especially at embryo transfer time. The aim of this review is to investigate what is currently known about the contribution of endometrial fluid (EF) to endometrial receptivity by identifying its potential biomarkers. Methods: This study involved an extensive search of the electronic databases PubMed and Cochrane, covering the period from 2011 to 2022. A combination of Medical Subject Headings with the terms 'endometrial fluid' and 'embryo implantation' was used. Results: Several different proteins presented in the endometrial cavity fluid have been described but the most consistent as potential biomarkers were Proprotein Convertase 6 (PC6), Vascular Endothelial Growth Factor (VEGF), PIGF (Placental growth factor), ß3 integrin, Colony Stimulating Factor-3 (CSF-3), Leukaemia inhibitory factor (LIF), glycodelin and extracellular vesicles (EVs). Conclusions: Strong indicators support the use of uterine fluid collection as a non-invasive tool for receptivity assessment. Therefore, it could improve outcomes of assisted reproductive techniques.

Plant growth-promoting bacteria in phytoremediation of metal-polluted soils: Current knowledge and future directions.

Soil metal contamination is a major concern due to the ever-rising number of areas afflicted worldwide and the detrimental effects of metals to the environment and human health. Due to their non-biodegradability and toxicity, it is paramount to prevent further metal contamination and remediate the thousands of contaminated sites across the planet. Yet, conventional reclamation based on physical and chemical methods is often expensive, impractical, and triggers secondary pollution issues. Hence, microbe-aided phytoremediation has been gaining significant traction due to its environment-friendly character, cost-effectiveness, and the breakthroughs achieved during the past few decades. Microorganisms are an essential part of natural ecosystems and play a crucial role in their restoration. Indeed, plant-microbe associations in metal-polluted soils are pivotal for plants to tolerate metal toxicity and thrive in these harsh environments. Therefore, improving the understanding of this intricate relationship is invaluable for boosting phytoremediation. In this review, we focus on the potential of plant growth promoting bacteria (PGPB) for enhancing phytoremediation of metal-polluted soils. We discuss the mechanisms employed by microbes to promote plant growth and assist the removal or immobilization of metals in soil, thereby enhancing phytoextraction and phytostabilization, respectively. Microbe-mediated metal removal and detoxification through processes entailing adsorption, chelation, transformation, and precipitation, to list but a few, are also critically examined. Moreover, this work covers the direct and indirect mechanisms used by PGPB to facilitate plant acquisition of nutrients like nitrogen and phosphorus, supply and regulate phytohormones, and exert control over antagonistic microorganisms. Lastly, we provide an outlook on the future directions of microbe-aided phytoremediation and phytomining. Clearly, to fully validate and comprehend the potential of PGPB-aided phytoremediation, a considerable shift from bench-scale to field research is necessary. What's more, it is envisaged that recent advancements in genetic engineering may soon help furthering the efficiency of microbe-assisted phytoremediation.

A differential diagnosis for a postpartum headache.

Headache is a common finding in the postpartum period. A wide variety of factors can contribute for its appearance and the causes include primary as well as secondary headache disorders. The postdural puncture is one of the most common headache causes in this context, but not the only one, which is why a differential diagnosis of postpartum headache is essential. We describe a patient with a headache in the immediate postpartum period. It was initially diagnosed as a common postdural puncture headache, but was later discovered to be a cervical hematoma.

Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri.

Aliarcobacter butzleri is an emergent enteropathogen, showing high genetic diversity, which likely contributes to its adaptive capacity to different environments. Whether natural transformation can be a mechanism that generates genetic diversity in A. butzleri is still unknown. In the present study, we aimed to establish if A. butzleri is naturally competent for transformation and to investigate the factors influencing this process. Two different transformation procedures were tested using exogenous and isogenic DNA containing antibiotic resistance markers, and different external conditions influencing the process were evaluated. The highest number of transformable A. butzleri strains were obtained with the agar transformation method when compared to the biphasic system (65% versus 47%). A. butzleri was able to uptake isogenic chromosomal DNA at different growth phases, and the competence state was maintained from the exponential to the stationary phases. Overall, the optimal conditions for transformation with the biphasic system were the use of 1 µg of isogenic DNA and incubation at 30 °C under a microaerobic atmosphere, resulting in a transformation frequency ~8 × 10-6 transformants/CFU. We also observed that A. butzleri favored the transformation with the genetic material of its own strain/species, with the DNA incorporation process occurring promptly after the addition of genomic material. In addition, we observed that A. butzleri strains could exchange genetic material in co-culture assays. The presence of homologs of well-known genes involved in the competence in the A. butzleri genome corroborates the natural competence of this species. In conclusion, our results show that A. butzleri is a naturally transformable species, suggesting that horizontal gene transfer mediated by natural transformation is one of the processes contributing to its genetic diversity. In addition, natural transformation can be used as a tool for genetic studies of this species.

High-Intensity Interval Training upon Cognitive and Psychological Outcomes in Youth: A Systematic Review.

Development of innovative and time-efficient strategies to involve youth in physical activity is pivotal in the actual inactivity pandemic. Moreover, physical activity may improve academic performance, of great interest for educators. This present systematic review aimed to analyze the effects of high-intensity interval training (HIIT) on cognitive performance and psychological outcomes in youth. A database search (Web of Science, PubMed, Scopus, and PsycINFO) for original research articles was performed. A total of eight articles met the inclusion criteria, and the Cochrane risk of bias tool was used. The studies' results were recalculated to determine effect sizes using Cohen's d. Different HIIT interventions reported improvements on cognitive performance at executive function (d = 0.75, +78.56%), linguistic reasoning (d = 0.25, +7.66%), concentration (d = 0.71, +61.10%), selective attention (d = 0.81, +60.73%), non-verbal and verbal abilities (d = 0.88, +47.50%; d = 1.58, +22.61%, respectively), abstract reasoning (d = 0.75, +44.50%), spatial and numerical abilities (d = 37.19, +22.85%; d = 1.20, +8.28%, respectively), and verbal reasoning (d = 1.00, +15.71%) in youth. Regarding psychological outcomes, HIIT showed higher self-concept (d = 0.28, +8.71%) and psychological well-being in boys and girls (d = 0.73, +32.43%, d = 0.39, +11.58%, respectively). To sum up, HIIT interventions between 4-16 weeks, for 8-30 min/session, at ≥85% maximal heart rate, would provide positive effects on cognitive performance and psychological outcomes in youth.

The Effect of Warm-up Running Technique on Sprint Performance.

ABSTRACT: Gil, MH, Neiva, HP, Alves, AR, Sousa, AC, Ferraz, R, Marques, MC, and Marinho, DA. The effect of warm-up running technique on sprint performance. J Strength Cond Res 35(12): 3341-3347, 2021-The purpose of the current study was to analyze the effect of changing the running technique during warm-up on sprint performances, running biomechanics, physiological, and psychophysiological responses. Thirty-one physically active men aged 18-23 years (mean ± SD: 19.35 ± 1.08 years of age; 1.77 ± 0.07 m of height; 71.90 ± 10.37 kg of body mass) volunteered to participate and randomly performed 2 maximal 30-m sprints, 5 minutes after completing a warm-up focused on increased stride length-SL (WUL) or a warm-up focused on increased stride frequency-SF (WUF). The results showed that there were no differences between the 30-m sprint performances and in running biomechanics. However, WUF showed increased performances in the first 15 m of the race (WUF: 2.59 ± 0.11 seconds vs. WUL: 2.63 ± 0.15 seconds; p = 0.03), and WUL resulted in higher performances in the last 15 m (1.94 ± 0.19 seconds vs. 1.88 ± 0.09 seconds; p = 0.05). In the second 30-m time trial, WUF also resulted in faster starting 15 m of the race (2.58 ± 0.12 seconds vs. 2.63 ± 0.16 seconds; p = 0.04). Interestingly, the WUF was the warm-up that revealed more stability in performances and running biomechanics between both trials. These results showed that there were no significant differences between warm-ups comprising exercises focusing in higher SL or higher SF in 30-m sprint biomechanics and performance. Nevertheless, different running strategies were caused by those 2 warm-ups and a more stabilized running pattern, and performance values were found when warm-up focused on higher SF.

Does the inclusion of ballistic exercises during warm-up enhance short distance running performance?

BACKGROUND: Warm-up is considered essential to optimize running performance, but little is known about the effect of specific warm-up tasks, specifically in the real competitive context. The current study aimed to verify the acute effects of a warm-up including ballistic exercises in 30m running performance. In addition, a second 30m trial was assessed to better understand the warm-up effects in training/competition. METHODS: Twenty-two men (19.32±1.43 years-old) randomly completed the time-trials on separate days and after a typical warm-up (WU), a WU complemented with ballistic exercises (postactivation potentiation [PAP]) or no warm-up (NWU). Biomechanical, physiological and psychophysiological variables were assessed. RESULTS: The participants were 1.9% faster in the first 30m sprint after WU compared with NWU, mainly increased performance in the first 15m (P=0.03, ES=0.48). WU resulted in greater stride length in the last 15m of the first sprint. PAP did not differ from NWU and WU, despite eight participants performed better after this warm-up. CONCLUSIONS: These results highlight the positive effects of warm-up for sprinting, despite failed to evidence positive effects when ballistic exercises are included. In addition, the influence of warm-up in the running technique was highlighted by the changes in the running kinematics and a need for individualization of warm-up procedures.

Modeling Fitness Variable Responses to Training in Prepubescent Children.

Alves, AR, Marta, C, Neiva, HP, Nunes, C, Izquierdo, M, and Marques, MC. Modeling fitness variable responses to training in prepubescent children. J Strength Cond Res 34(8): 2352-2359, 2020-The aim of this study was to determine strength and oxygen uptake (V[Combining Dot Above]O2max) performances according to different training program intervention design with 8-week duration in prepubescent children through a multiple linear regression models. Two hundred forty-five healthy prepubescent children (aged 10.9 ± 0.5 years) were randomly assigned to a specific training program (strength training only-S; aerobic training only-A; intrasession aerobic and strength training-AS; intrasession strength and aerobic training-SA; or concurrent training performed in different sessions-CT) or a control group (no training regimen-C). It was possible to develop indirect predictive models for each training method, by including each variable pretraining, body fat percentage and body mass index. The models provided explained 82% of variance in the V[Combining Dot Above]O2max, 98% in the 1 kg ball-throw, 96% in the 3 kg ball-throw, 92% in the countermovement jump, 93% in the standing long jump and 98% in the 20 m sprint performances. This novel approach to training evaluation and control aims to provide a tool to allow professionals to calculate changes with a high confidence level (CI 95%), to control gains and to choose the best training methodology to apply according to the defined purposes. The results of this study could be a great support to teachers, coaches, and professionals providing important tools to improve the efficacy and individualization of training.