The Advancing Role of Nanocomposites in Cancer Diagnosis and Treatment.

The relentless pursuit of effective cancer diagnosis and treatment strategies has led to the rapidly expanding field of nanotechnology, with a specific focus on nanocomposites. Nanocomposites, a combination of nanomaterials with diverse properties, have emerged as versatile tools in oncology, offering multifunctional platforms for targeted delivery, imaging, and therapeutic interventions. Nanocomposites exhibit great potential for early detection and accurate imaging in cancer diagnosis. Integrating various imaging modalities, such as magnetic resonance imaging (MRI), computed tomography (CT), and fluorescence imaging, into nanocomposites enables the development of contrast agents with enhanced sensitivity and specificity. Moreover, functionalizing nanocomposites with targeting ligands ensures selective accumulation in tumor tissues, facilitating precise imaging and diagnostic accuracy. On the therapeutic front, nanocomposites have revolutionized cancer treatment by overcoming traditional challenges associated with drug delivery. The controlled release of therapeutic agents from nanocomposite carriers enhances drug bioavailability, reduces systemic toxicity, and improves overall treatment efficacy. Additionally, the integration of stimuli-responsive components within nanocomposites enables site-specific drug release triggered by the unique microenvironment of the tumor. Despite the remarkable progress in the field, challenges such as biocompatibility, scalability, and long-term safety profiles remain. This article provides a comprehensive overview of recent developments, challenges, and prospects, emphasizing the transformative potential of nanocomposites in revolutionizing the landscape of cancer diagnostics and therapeutics. In Conclusion, integrating nanocomposites in cancer diagnosis and treatment heralds a new era for precision medicine.

A Review of Interactions between Plants and Whitefly-Transmitted Begomoviruses.

The transmission of plant viruses from infected to healthy host plants is a process in which insects play a major role, using various transmission strategies. Environmental factors have an impact on the transmission of viruses and the subsequent development of infections or diseases. When viruses are successful, plant virus diseases can reach epidemic proportions. Many plants across different regions are vulnerable to viral infections transmitted by the whitefly vector. Begomoviruses, which are transmitted by whiteflies, represent a significant threat to agriculture worldwide. The review highlights the mechanisms of virus acquisition and transmission by whiteflies and explores the factors influencing these interactions. Understanding the impacts of these changes is crucial for managing the spread of pests and mitigating damage to crops. It underscores the need for continued research to elucidate the mechanisms driving plant-insect-virus interactions and to identify new approaches for sustainable pest management.

Cytotoxicity of AuCu-Cu2S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines.

AuCu-Cu2S nanocomposites are unique materials with exceptional properties that have recently received a lot of interest. However, little is known about their potential toxicity in terrestrial organisms and their subsequent effects on the environment. Therefore, it is essential to develop effective methodologies for evaluating AuCu-Cu2S nanocomposites in biological systems. This study reports the biological evaluation of the AuCu-Cu2S nanocomposite from animal and cell entity levels. The Bombyx mori silkworm was used as a model organism to study the effects of different concentrations of AuCu-Cu2S on silkworm development. Transcriptome analysis was also carried out to examine the genetic modulation exerted by the treatment. Moreover, biocompatibility and cytotoxicity of AuCu-Cu2S were evaluated in human bronchial epithelial cells 16HBE, human lung adenocarcinoma, and the insect Spodoptera frugiperda cell sf9 cell lines. The results showed that although AuCu-Cu2S at ≤400 ppm can prolong the eating habit of silkworms and promote the weight of the cocoon layer, there was an increase in silkworm mortality and a decrease in moth formation at a concentration of ≥800 ppm. The genetic regulation by AuCu-Cu2S treatment showed varying effects in the silkworm, primarily related to functions such as transport and catabolism, metabolism of cofactors and vitamins, xenobiotic biodegradation, amino acid, and carbohydrate. 16HBE, PC-9, and sf9 treated with 300 ppm of AuCu-Cu2S showed viability percentages of 60, 20, and 90%, respectively. Thus, AuCu-Cu2S at low concentrations serves as a safe and biocompatible material for the sf9 cell lines but is lethal to 16HBE and PC-9. This research could aid in understanding the biological effects and biocompatibility of AuCu-Cu2S nanocomposites, particularly in the field of biochemistry; however, the mechanisms involved need further exploration.

Sustainable Pest Management in Date Palm Ecosystems: Unveiling the Ecological Dynamics of Red Palm Weevil (Coleoptera: Curculionidae) Infestations.

The red palm weevil (RPW) poses a significant threat to date palm ecosystems, highlighting the necessity of sustainable pest management strategies that carefully consider the delicate ecological balance within these environments. This comprehensive review delves into innovative approaches to sustainable pest management, specifically focusing on date palm, and seeks to unravel the intricate ecological dynamics underlying RPW infestations. We thoroughly analyze biocontrol methods, eco-friendly chemical interventions, and integrated pest management (IPM) strategies, aiming to minimize the ecological impact while effectively addressing RPW infestations. By emphasizing the interplay of both living organisms (biotic) and environmental factors (abiotic) in shaping RPW dynamics, we advocate for a holistic and sustainable management approach that ensures the long-term resilience of date palm ecosystems. This review aims to contribute to an ecologically sound framework for pest management, promoting the sustainability and vitality of date palm ecosystems amidst the challenges posed by the RPW.

Multi-omics study and ncRNA regulation of anti-BmNPV in silkworms, Bombyx mori: an update.

Bombyx mori silkworm is an important economic insect which has a significant contribution to the improvement of the economy. Bombyx mori nucleopolyhedrovirus (BmNPV) is a vitally significant purulent virus that impedes the sustainable and stable development of the silkworm industry, resulting in substantial economic losses. In recent years, with the development of biotechnology, transcriptomics, proteomics, metabolomics, and the related techniques have been used to select BmNPV-resistant genes, proteins, and metabolites. The regulatory networks between viruses and hosts have been gradually clarified with the discovery of ncRNAs, such as miRNA, lncRNA, and circRNA in cells. Thus, this paper aims to highlight the results of current multi-omics and ncRNA studies on BmNPV resistance in the silkworm, providing some references for resistant strategies in the silkworm to BmNPV.

The Influence of the Size of BN NSs on Silkworm Development and Tissue Microstructure.

Boron nitride nanosheets (BN NSs) have emerged as promising materials in a wide range of biomedical applications. Despite the extensive studies on these bio-nano interfacial systems, one critical concern is their toxicity, which is affected by a variety of factors, including size. This study aimed at assessing the relationship between BN NSs size and toxicity. Two silkworm strains (qiufeng × baiyu and Nistari 7019) were used as model organisms to investigate the effect of different sizes of BN NSs (BN NSs-1, thickness of 41.5 nm and diameter of 270.7 nm; BN NSs-2, thickness of 48.2 nm and diameter of 562.2 nm) on silkworm mortality, growth, cocoon weight, and tissue microstructure. The findings show that exposure to BN NSs in this work has no lethal adverse effects on silkworm growth or tissue microstructure. BN NSs have a higher effect on the growth rate of qiufeng × baiyu compared to Nistari 7019, demonstrating that the same treatment does not favorably affect the Nistari 7019 strain, as there is no significant increase in cocoon weight. Overall, the study suggests that the sizes of BN NSs employed in this study are relatively safe and have less negative impact on silkworms. This offers significant insights into the effect of BN NSs size, a crucial factor to consider for their safe use in biomedical applications.

The Mechanism of Hyperglycemia-Induced Renal Cell Injury in Diabetic Nephropathy Disease: An Update.

Diabetic Nephropathy (DN) is a serious complication of type I and II diabetes. It develops from the initial microproteinuria to end-stage renal failure. The main initiator for DN is chronic hyperglycemia. Hyperglycemia (HG) can stimulate the resident and non-resident renal cells to produce humoral mediators and cytokines that can lead to functional and phenotypic changes in renal cells and tissues, interference with cell growth, interacting proteins, advanced glycation end products (AGEs), etc., ultimately resulting in glomerular and tubular damage and the onset of kidney disease. Therefore, poor blood glucose control is a particularly important risk factor for the development of DN. In this paper, the types and mechanisms of DN cell damage are classified and summarized by reviewing the related literature concerning the effect of hyperglycemia on the development of DN. At the cellular level, we summarize the mechanisms and effects of renal damage by hyperglycemia. This is expected to provide therapeutic ideas and inspiration for further studies on the treatment of patients with DN.

Metabolomics of Esophageal Squamous Cell Carcinoma Tissues: Potential Biomarkers for Diagnosis and Promising Targets for Therapy.

Background: The incidence of esophageal squamous cell carcinoma in China ranks first in the world. The early diagnosis technology is underdeveloped, and the prognosis is poor, which seriously threatens the quality of life of the Chinese people. Epidemiological findings are related to factors such as diet, living habits, and age. The specific mechanism is not clear yet. Metabolomics is a kind of omics that simultaneously and quantitatively analyzes the comprehensive profile of metabolites in living systems. It has unique advantages in the study of the diagnosis and pathogenesis of tumor-related diseases, especially in the search for biomarkers. Therefore, it is desirable to perform metabolic profiling analysis of cancer tissues through metabolomics to find potential biomarkers for the diagnosis and treatment of esophageal squamous cell carcinoma. Methods: HPLC-TOF-MS/MS technology and Illumina Hiseq Xten Sequencing was used for the analysis of 210 pairs of matched esophageal squamous cell carcinoma tissues and normal tissues in Zhenjiang City, Jiangsu Province, a high-incidence area of esophageal cancer in China. Bioinformatics analysis was also performed. Results: Through metabolomic and transcriptomic analysis, this study found that a total of 269 differential metabolites were obtained in esophageal squamous cell carcinoma and normal tissues, and 48 differential metabolic pathways were obtained through KEGG enrichment analysis. After further screening and identification, 12 metabolites with potential biomarkers to differentiate esophageal squamous cell carcinoma from normal tissues were obtained. Conclusions: From the metabolomic data, 4 unknown compounds were found to be abnormally expressed in esophageal squamous cell carcinoma for the first time, such as 9,10-epoxy-12,15-octadecadienoate; 3 metabolites were found in multiple abnormal expression in another tumor, but upregulation or downregulation was found for the first time in esophageal cancer, such as oleoyl glycine; at the same time, it was further confirmed that five metabolites were abnormally expressed in esophageal squamous cell carcinoma, which was similar to the results of other studies, such as PE.

The Evaluation of the Biological Effects of Melanin by Using Silkworm as a Model Animal.

Melanin has been reported to have potential applications in industries such as cosmetics and food due to its anti-UV and antioxidative qualities. However, the corresponding data on its safety evaluation or biological consequences are fairly limited; such data are critical given its widespread use. The effect of different concentrations (1, 2, 3, and 4%) of melanin on growth status (larvae length and weight, cocoon weight, and morphology), the microstructure of the various tissues (fat body, silk gland, and midgut), and silk properties was studied by using the silkworm (bombyx mori) as the model organism. The weight and length of silkworm larvae fed with melanin were lower than the control, indicating that melanin appears to have a negative effect on the growth status of silkworms; however, the histophysiology analysis indicates that the cell morphologies are not changed, the XRD and FTIR spectra indicate that the secondary and crystalline structures of silks are also well preserved, and the thermogravimetric analysis and tensile test indicate that the thermal stability and mechanical properties are well maintained and even improved to some extent. Generally, it indicates that melanin has a certain inhibitory effect on the growth of silkworm larva but causes no harm to the cell microstructures or silk properties; this demonstrates that the safety of melanin as a food addictive should be considered seriously. The increase of thermal stability and mechanical properties shows that melanin may be a good chemical modifier in textile industries.

Subchronic toxicity of magnesium oxide nanoparticles to Bombyx mori silkworm.

Despite many research efforts devoted to the study of the effects of magnesium oxide nanoparticles (MgO NPs) on cells or animals in recent years, data related to the potential long-term effects of this nanomaterial are still scarce. The aim of this study is to explore the subchronic effects of MgO NPs on Bombyx mori silkworm, a complete metamorphosis insect with four development stages (egg, larva, pupa, month). With this end in view, silkworm larvae were exposed to MgO NPs at different mass concentrations (1%, 2%, 3% and 4%) throughout their fifth instar larva. Their development, survival rate, cell morphology, gene expressions, and especially silk properties were compared with a control. The results demonstrate that MgO NPs have no significant negative impact on the growth or tissues. The cocooning rate and silk quality also display normal results. However, a total of 806 genes are differentially expressed in the silk gland (a vital organ for producing silk). GO (Gene Ontology) results show that the expression of many genes related to transporter activity are significantly changed, revealing that active transport is the main mechanism for the penetration of MgO NPs, which also proves that MgO NPs are adsorbed by cells. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis demonstrates that the longevity regulating pathway-worm, peroxisome and MAPK signaling pathway are closely involved in the biological effects of MgO NPs. Overall, subchronic exposure to MgO NPs induced no apparent negative impact on silkworm growth or silks but changed the expressions of some genes.

In vivo toxicity evaluation of boron nitride nanosheets in Bombyx mori silkworm model.

Boron nitride nanosheets (BN NSs), a novel material with a structure similar to graphene, have attracted much attention due to their extraordinary properties. A deep in vivo study of the toxicity of BN NSs is indispensable, which can help to understand their potential risk and provide useful information for their safe application. However, so far as we know, the systematic in vivo toxicity evaluation of BN NSs hasn't been reported. In this study, silkworm (Bombyx mori) was used as a model to investigate the toxicity of BN NSs, by continuously feeding silkworm larvae with BN NSs at various mass concentrations (1%, 2%, 3%, 4%). The toxicity was evaluated from the levels of animal entirety (mortality, silkworm growth, cocoons and silk properties), tissues (pathological examination) and genes (transcriptomic profiling). The results show that the exposure to BN NSs causes no obvious adverse effects on the growth, silk properties or tissues of silkworm, but the expressions of genes in midgut concerned with some specific functions and pathways are significantly changed, indicating that BN NSs may have potential danger to lead to dysfunction. This study has performed in vivo toxicity evaluation of BN NSs and provided useful safety information for the application of BN NSs.