Asunto(s)
Escoliosis , Metilación de ADN , Femenino , Humanos , Regiones Promotoras Genéticas , Escoliosis/genéticaRESUMEN
Endometriosis, the presence of ectopic endometrium, has an unclear etiology and is commonly associated with endocrine, genetic, and immunological imbalance. This study determined whether immunomodulation by the RESAN vaccine could alter the potentially pathogenic gene expression profiles in the cells of the eutopic endometrium in an animal model of endometriosis. Preventing these changes could inhibit the early development of the illness and support the success of surgical treatment. Wistar rats were divided into three groups: prophylaxis (vaccinated before ectopic endometrium implantation, n = 23), therapeutic (vaccinated at the time of the ectopic excision, n = 23) and control (n = 10). During the first laparotomy, autotransplantation of the endometrium to the peritoneum was performed in the prophylaxis and therapeutic groups. The second laparotomy was carried out three months later in all groups to examine endometriotic foci and adhesions. Suspected endometriosis foci were removed. Three months later, the third laparotomy was performed in all animals, followed by suspected foci excision. Fragments of the eutopic endometrium were collected from all animals during the first and third laparotomies. All samples were analysed by real-time PCR to assess the expression of Bcl2, Bax, Bax/Bcl2 ratio, Mki67, and Tert genes. Endometrial foci were found in abdominal peritoneum at the second laparotomy in 1 animal in the prophylaxis group, compared to 16 animals in the therapeutic group. The prophylaxis group showed a high expression of Bax while the therapeutic group showed high expression of Bax, Tert and Mki67 genes. Additional analysis revealed that throughout the six months of the experiment, the expression of the Bax, Tert, and Mki67 genes decreased significantly in the prophylaxis group, Mki67 gene expression decreased in the therapeutic group, and Tert, Mki67, and Bcl2 gene expression decreased in the control group. The results indicate that immunomodulation affects the balance between apoptosis and proliferation in the eutopic endometrium and may prevent the onset and recurrence of endometriosis.
Asunto(s)
Vacunas contra el Cáncer/administración & dosificación , Endometriosis/inmunología , Endometriosis/prevención & control , Inmunomodulación/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Apoptosis/fisiología , Pollos , Endometriosis/metabolismo , Endometrio/efectos de los fármacos , Endometrio/inmunología , Endometrio/metabolismo , Femenino , Inmunomodulación/fisiología , Ratas , Ratas WistarRESUMEN
A considerable number of biochemical and physiologic studies evaluate the roles of gonadotropins in carcinogenesis. Latest reports show that human chorionic gonadotropin (hCG), and especially its beta subunit, are secreted by a variety of malignant tumors of different origin. However, the mechanism of hCG action and its role in tumor development is not known yet. This study, with the help of reverse transcription-polymerase chain reaction and immunohistochemistry, is an attempt to document the molecular presence of the hCGbeta and luteinizing hormone/hCG receptor (LH/hCGR) in the ovarian, endometrial, and uterine cervix cancer tissues. The LH/hCGR, coexpressed with hCGbeta, may act as a potential mediator of hCG action in nontrophoblastic gynecological cancers.
Asunto(s)
Gonadotropina Coriónica Humana de Subunidad beta/metabolismo , Regulación Neoplásica de la Expresión Génica , Neoplasias Ováricas/metabolismo , Receptores de HL/metabolismo , Neoplasias Uterinas/metabolismo , Gonadotropina Coriónica Humana de Subunidad beta/genética , Femenino , Humanos , Inmunohistoquímica , Neoplasias Ováricas/genética , Neoplasias Ováricas/patología , Receptores de HL/genética , Neoplasias Trofoblásticas/genética , Neoplasias Trofoblásticas/metabolismo , Neoplasias Uterinas/genética , Neoplasias Uterinas/patologíaRESUMEN
The purpose of the present investigation was to determinate expression of human chorionic gonadotropin gene in ovarian cancer tissue. The study included 15 patients with epithelial ovarian carcinoma. The expression of mRNA hCGbeta was determinated by the RT PCR method and the distribution of the hormone in study tissue was analyzed immunohistochemicaly. In all 15 study specimens of the ovarian carcinoma tissue the active hCGbeta gene was found, whereas noncancerous tissue demonstrated lack of the hormone expression. Thus, the study clearly shows that the expression of hCGbeta is the feature of ovarian cancer tissue.
Asunto(s)
Gonadotropina Coriónica Humana de Subunidad beta/genética , Regulación Neoplásica de la Expresión Génica , Predisposición Genética a la Enfermedad , Neoplasias Ováricas/genética , Neoplasias Ováricas/patología , Secuencia de Bases , Biopsia con Aguja , Estudios de Casos y Controles , Femenino , Humanos , Inmunohistoquímica , Datos de Secuencia Molecular , Estadificación de Neoplasias , Pronóstico , ARN Mensajero/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Muestreo , Sensibilidad y EspecificidadRESUMEN
Involvement of variety of genes, especially located on Y chromosome, is critical for the regulation of spermatogenesis. In particular, fertility candidate genes such as deleted in azoospermia (DAZ) are believed to have important function in sperm production, since DAZ is frequently deleted in azoospermic and severy oligozoospermic men. The role of the DAZ gene is supported by its exclusive expression in the testis and by its deletion in about 10% of azoospermic and severely oligozoospermic patients. The distribution of DAZ transcripts in seminiferous epithelium of human testis is reported in the present study. The use of Adobe Photoshop and Scion Image softwares allowed for semi-quantitative analysis of in situ RT-PCR (ISRT-PCR) results. The intensity of ISRT-PCR product's fluorescence was different within individual seminiferous tubules. It was clearly shown by using the pseudocolour scale and transforming the intensity of the fluorescence into levels of greyscale images. The more intense fluorescence characterised single spermatogonia and those organized in small groups inside separate tubules. The most intense accumulation of DAZ mRNA was observed in spermatogonia.
Asunto(s)
Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/metabolismo , Túbulos Seminíferos/metabolismo , Testículo/metabolismo , Proteína 1 Delecionada en la Azoospermia , Fertilidad/genética , Expresión Génica , Humanos , Procesamiento de Imagen Asistido por Computador/métodos , Masculino , Microscopía Fluorescente , ARN Mensajero/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Túbulos Seminíferos/citología , Espermatogénesis/genética , Espermatogonias/citología , Espermatogonias/metabolismo , Testículo/citologíaRESUMEN
PURPOSE: Determination of the correlation between expression of human chorionic gonadotropin mRNA and serum free hCGb immunoreactivity in endometrial cancer tissue. METHODS: The study included 56 patients with endometrial carcinoma Stages IB-III. The expression of mRNA hCGbeta was determined by the RT PCR method in 18 cases of cancerous and precancerous tissues. The serum-free hCGbeta immunoreactivity was analyzed by sequential immunometric assay in all patients. RESULTS: In 15 study specimens of endometrial carcinoma tissue mRNA of hCGbeta was detected. Also in endometrial atypical hyperplasia, expression of hCGbeta was found. Noncancerous tissue demonstrated lack of the hCGbeta transcript. The serum immunoreactivity in the endometrial cancer group was detectable in 86% of cases. There were no significant differences between FIGO stages and grading. CONCLUSION: The results of the present study confirmed the presence of active genes of hCGbeta in endometrial cancer tissue, even in precancerous changes. The serum immunoreactivity of free hCGbeta is a less common feature and is not linked with tumor stage or grade in endometrial cancer patients.