New Insight of Scaffold Based on Hydroxyapatite (HAp)/Bacteria's Nanocellulose (BN) for Dental Tissue Engineering.

OBJECTIVE: Bacterial nanocellulose (BN), derived from Acetobacter xylinum ATCC 237672, is a polymer that offers several desirable characteristics for scaffolds applications. To further enhance the characteristic of the BN scaffold, hydroxyapatite (HAp) from Anadara granosa and Achatina fulica can be incorporated. Therefore, the aim of the study was to characterize the physical properties of a three-dimensional (3D) scaffold made of HAp and BN. MATERIALS AND METHODS: The scaffold was developed using the cellulose immersion technique, where BN was soaked in HAp suspension for different duration (5, 10, 15, 20, and 25 hours). The physical properties that were evaluated included porosity, pore density, swelling ratio, and water retention. RESULTS: The HAp/BN 3D scaffold, which is considered a hydrogel material, exhibited favorable physical properties that can support cell survival. The total porosity of the scaffolds was 100%. There was no significant difference porosity among the groups (p > 0.05). The swelling ratio increased on day 1 and then sharply decreased on day 2. There was a significant difference between the groups on both day 1 and day 2 (p < 0.05). The scaffolds immersed in the HAp for more than 15 hours exhibited higher water retention compared to the other groups, and there was a significant difference between the groups on day 2 and day 4 (p < 0.05). The scaffold immersed for more than 15 hours exhibited a higher pore density compared to those immersed for less than 15 hours, and there was no a significant difference between the groups (p > 0.05). CONCLUSION: Our findings suggest that the HAp/BN 3D scaffold, especially when immersed in HAp for 15 hours, possesses promising physical properties that make it suitable for various applications in dental tissue engineering.

PCL/Graphene Scaffolds for the Osteogenesis Process.

This study aims to characterize the osteoconductivity, optimal bioresorbable, biodegradability, biocompatibility, and mechanical properties of Poly-ε-caprolactone (PCL)/graphene (G) scaffolds at concentrations of 0.5, 1, 1.5, 2, 2.5, and 3 wt%, which are used to support bone regeneration through solvent casting and particulate leaching. The water contact angle measurement revealed a transition from a hydrophobic to a hydrophilic surface after incorporating various G concentrations. The scaffolds with 0.5 wt% G had smaller pores compared to those produced using 3 wt% G. Furthermore, numerous pores were connected, particularly those with larger diameters in the 2 and 3 wt% G samples. The proportion of water absorption varied between 50% and 350% for 4 months, with large percentages of scaffolds containing high G concentrations. Raman spectroscopy and X-ray diffraction, which were used to confirm the presence of nanofiller by increasing the ratios of ID/IG, I2D/IG, and band 2θ = 26.48°. The mechanical properties were improved by the addition of G, with a Young's modulus of 3 wt% G, four times that of PCL. Measuring cell biocompatibility, adhesion, proliferation, and differentiation with osteoblast-like (MG-63) cells revealed that PCL/G scaffolds with higher concentrations were more biocompatible than PCL as well as those with lower concentrations.

Novel Application of 3D Scaffolds of Poly(E-Caprolactone)/Graphene as Osteoinductive Properties in Bone Defect.

OBJECTIVE: Scaffolds provided a surface on which cells could attach, proliferate, and differentiate. Nowadays, bone tissue engineering offers hope for treating bone cancer. Poly(e-caprolactone) (PCL)/graphene have capability as an osteogenic and regenerative therapy. It could be used to produce bone tissue engineering scaffolds. The purpose of this study was to investigate the ability of PCL/graphene to enhance the osteoinductive mechanism. MATERIALS AND METHODS: The PCL/graphene scaffold was developed utilizing a particulate-leaching process and cultured with osteoblast-like cells MG63 at 0.5, 1.5, and 2.5 wt% of graphene. We evaluated the porosity, pore size, migratory cells, and cell attachment of the scaffold. STATISTICAL ANALYSIS: Data was expressed as the mean ± standard error of the mean and statistical analyses were performed using one-way analysis of variance and Tukey's post hoc at a level of p-value < 0.05. RESULTS: Porosity of scaffold with various percentage of graphene was nonsignificant (p > 0.05). There were differences in the acceleration of cell migration following wound closure between groups at 24 hours (p < 0.01) and 48 hours (p < 0.00). Adding the graphene on the scaffolds enhanced migration of osteoblast cells culture and possibility to attach. Graphene on 2.5 wt% exhibited good characteristics over other concentrations. CONCLUSION: This finding suggests that PCL/graphene composites may have potential applications in bone tissue engineering.

Estimation of Platelet Count and Bleeding Time of Mice Treated with Musa paradisiaca var. sapientum (L.) Kuntze Extract.

Objective: The aim of this study was to estimate the platelet count and bleeding time on peripheral blood smear of mice tail wound using Musa paradisiaca var. sapientum (L.) Kuntze (ambonese banana stem extract). Design: Randomized post-test-only control group design. Materials and Methods: Twenty-four male mice (Mus Musculus) were randomly divided into 4 groups. A negative control group was treated with carboxymethyl cellulose (CMC), a positive control group (K+) treated aspirin 100 mg/kg body weight, group P1 treated with aspirin 100 mg/kg body weight and tranexamic acid 50 mg/kg body weight, and group P2 treated with 30% of ambonese banana stem extract (ABSE). The mean and standard deviation data of platelet counts and bleeding time were analyzed by one-way ANOVA statistical software. Results and Discussion: Tranexamic acid had no significant effect on platelets count compared to CMC group (p = 0.871), but administration of aspirin resulted in low platelets count significantly (p = 0.003). The platelet counts of ABSE and CMC groups were not significant different (p = 0.937). Aspirin has significantly shown prolonged bleeding time than CMC, tranexamic acid, and ABSE groups. However, there was no difference between the tranexamic acid and ABSE groups (p=0.934). The bleeding time of tranexamic acid and ABSE groups was similar, although the platelet count in the ABSE group was lower than in the CMC group. Conclusion: This study proved that ambonese banana stem extract has a potency to shorten the bleeding time in mice tail wound without interfering to platelet count.

Topical Medicine Potency of Musa paradisiaca var. sapientum (L.) kuntze as Oral Gel for Wound Healing: An In Vitro, In Vivo Study.

OBJECTIVE: Topical application of ambonese banana (Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of this study is to achieve standard formulation of GEGPA through stability, viscosity, distribution area, and drugs release for oral gel wound healing. MATERIALS AND METHODS: This is an in vitro and in vivo study with the randomized posttest only control group design. The gel was formulated according to the composition of each group by adding hydroxypropyl methylcellulose (HPMC), Lexgard, propylene glycol, and cold water to obtain 100 g of gel. Observations were made through the following tests: stability, viscosity, distribution area, drug release, and histopathological analysis of tooth extraction wound healing. STATISTICAL ANALYSIS: Data were analyzed using a one-way analysis of variance (α = 0.05) with GraphPad Prism-8 statistical software. RESULTS: The study showed that the GEGPA formulation was stable against changes in consistency, color, smell, homogeneity, and pH value. There is a significant difference between groups with respect to viscosity (p = 0.0001), adhesion (p = 0.004), dispersion (p = 0.000), and fibroblast cell numbers on days 3 and 5 (p = 0.007 and p = 0.001). There is no interaction between the active ingredients and the gel base of all formulations. Formulation 3 had better properties in terms of viscosity, broad distribution, and drug release compared with other groups. Application of GEGPA to tooth extraction wounds showed a significant proliferation of fibroblast cells on days 3 and 5. CONCLUSIONS: The formulation of M. paradisiaca var. sapientum (L.) kuntze extract with HPMC and propylene glycol obtained a gel preparation, GEGPA, that was organoleptically stable and met the topical gel standard for wounds in the oral cavity.

Determining the Effectivity of Infrared Distance to Eliminate Dental Pain Due to Pulpitis and Periodontitis.

OBJECTIVE: The infrared rays is one of the treatments to relief of dental pain due to pulpitis or periodontitis. The ability of infrared to increase the pain threshold which make eliminating P substance on the inflammation area and inducing Aß and Aδ fibers to activate γ-aminobutyrate (GABA) and neuropeptides to decrease the pain. Hence, reducing or eliminating dental pain. This study to get information about effectively distance between patients and infrared rays which can reduce or eliminate dental pain. MATERIALS AND METHODS: The explorative experimental research to determine the effectivity of the infrared rays' distance: 45, 55, and 55 cm in reducing or eliminating dental pain. The material of infrared was tungsten with luminous light and the time for the lighting was 15 minutes. STATISTICAL ANALYSIS: The data were obtained and analyzed using Wilcoxon's signed rank test and Kruskal-Wallis test (α= 0.05). RESULTS: There were influenced of the infrared rays to reduce the dental pain as follow, distance 45 cm (p = 0.007), distance 55 cm (p = 0.026), and distance 65 cm (p = 0.007). The average scale reduction for distance 45 cm was 2.23 ± 0.83, the distance 55 cm was 3.33 ± 0.87, and the distance 65 cm was (1.78 ± 0.83). Therefore, according to Kruskal-Wallis test with p = 0.004 (p < 0.05) showed the significant difference between the scale to decrease of dental pain was the distance of 55. CONCLUSION: Infrared rays located at a distance of 55 cm from patients might be more effective in relieving dental pain, compared with other distances tested.

ANTIBACTERIAL ACTIVITY OF DRACONTOMELON DAO EXTRACTS ON METHICILLIN-RESISTANT S. AUREUS (MRSA) AND E. COLI MULTIPLE DRUG RESISTANCE (MDR).

BACKGROUND: Staphylococcus aureus, methicillin-resistant and Escherichia coli, multidrug-resistant included in the list of antibiotic-resistant priority pathogens from WHO. As multidrug-resistant bacteria problem is increasing, it is necessary to probe new sources for identifying antimicrobial compounds. Medicinal plants represent a rich source of antimicrobial agents. One of the potential plants for further examined as antibacterial is Dracontomelon dao (Blanco) Merr. & Rolfe. The present study designed to find the antibacterial activity of D. dao stem bark extracts on Methicillin-resistant S. aureus (MRSA) and E. coli Multiple Drug Resistance (MDR), followed by determined secondary metabolites with antibacterial activity and determined the value of MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration). MATERIALS AND METHODS: D. dao stem bark extracted using 60% ethanol. Disc diffusion test methods used to find the antibacterial activity, following by microdilution methods to find the value of MIC and MBC. Secondary metabolites with antibacterial activity determined by bioautography using TLC (thin layer chromatography) methods. RESULTS: D. dao stem bark extracts are sensitive to MSSA, MRSA and E.coli MDR bacteria. The inhibition zone is 16.0 mm in MSSA, 11.7 mm in MRSA and 10.7 mm in E. coli MDR. The entire MBC/MIC ratios for MSSA, MRSA and E.coli MDR is lower than 4. The ratio showed bactericidal effects of D. dao stem bark extracts. In TLC results, colorless bands found to be secondary metabolites with antibacterial activity. CONCLUSION: D. dao stem bark extracts are potential to develop as antibacterial agent especially against MRSA and E. coli MDR strain.