Toxicity of an Annonin-Based Commercial Bioinsecticide Against Three Primary Pest Species of Stored Products.

The effects of a bioinsecticide formulation based on extract of Annona squamosa L. (Annonaceae) containing 10,000 mg L-1 of acetogenin annonin as the main active ingredient were investigated against three primary pest species of stored grains in Brazil [maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), Mexican bean weevil Zabrotes subfasciatus (Boheman) (Coleoptera: Chrysomelidae: Bruchinae), and cowpea weevil Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae: Bruchinae)] by means of residual contact bioassays. In a concentration-dependent manner, the annonin-based commercial bioinsecticide caused significant adult mortality of C. maculatus (LC50 = 6890 µL kg-1), S. zeamais (LC50 = 2781 µL kg-1), and Z. subfasciatus (LC50 = 2120 µL kg-1) after 120 h of residual contact exposure. In addition to acute toxicity, the tested bioinsecticide also promoted a significant reduction of the number of eggs laid by females of C. maculatus (EC50 = 5949.7 µL kg-1) and Z. subfasciatus (EC50 = 552.7 µL kg-1). Moreover, the bioinsecticide significantly reduced the number of emerged insects (F1 generation) of C. maculatus (EC50 = 2763.0 µL kg-1), S. zeamais (EC50 = 1380.8 µL kg-1), and Z. subfasciatus (EC50 = 561.5 µL kg-1). The bioinsecticide also reduced the percentage of damaged grains for the three pest species studied, and its grain-protectant properties are comparable to or superior in efficacy in relation to a diatomaceous earth-based insecticide (Insecto® at 1000 mg kg-1) used as a positive control. Thus, this standardized formulation has promising bioactivity against stored insect species and can be a useful component for IPM of stored grains in Brazil and elsewhere.

Acute and Chronic Toxicities of an Annonin-Based Commercial Bioinsecticide and a Joint Mixture with a Limonoid-Based Formulation to the Fall Armyworm.

The bioactivity of a newly registered annonin-based formulation (Anosom® 1 EC) was investigated for use against the fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), both singly and mixed with a limonoid-based formulation (Azamax® 1.2 EC) using dietary exposure bioassays. Anosom® 1 EC caused significant mortality in S. frugiperda larvae exposed to treated artificial media, with the activity level dependent on both the concentration and the exposure time. In addition to exhibiting acute toxicity, this formulation (tested at LC50) also caused growth inhibition of S. frugiperda, as demonstrated by a significant reduction in pupal weight and viable larval and pupal phases and an increase in the duration of the larval stage. Moreover, the bioinsecticide caused an increase in the proportion of deformed or defective pupae; these were characterized by the retention of exuvia from the last larval instar. The bioinsecticide (at the previously estimated LC90) was, in a time-dependent manner, equally as toxic as the limonoid-based biopesticide (Azamax® 1.2 EC), which was tested at concentrations recommended for the management of S. frugiperda in Brasil. Moreover, the binary mixture (1/2 LC90 of Anosom® 1 EC + 1/2 the recommended rate of Azamax® 1.2 EC) of both bioinsecticides caused a similar level of mortality compared to the bioinsecticides tested individually (full concentration), demonstrating an additive effect of these commercial bioinsecticides. In light of these findings, this standardized formulation based on extract of Annona squamosa can constitute a useful component in the framework of S. frugiperda management, mainly in organic food production systems.

Pimenta pseudocaryophyllus Derivatives: Extraction Methods and Bioactivity Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae).

Plant-based insecticides can play an important role in integrated insect pest management (IPM), especially in protecting stored grains. The aim of this study was to evaluate the bioactivity of derivatives (powder, ethanolic extract, and essential oil (EO)) from the leaves of Pimenta pseudocaryophyllus (Myrtaceae), a Brazilian native species, against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), the main insect pest of stored corn. The powder and essential oil prepared from leaves showed a repellent effect. Moreover, the EO exhibited promising insecticidal activity through residual contact (LC50 = 1522 mg kg(-1)) and significantly decreased the F 1 progeny and the percentage of damaged grains. However, the essential oil obtained from P. pseudocaryophyllus leaves did not result in significant mortality of S. zeamais adults after 72 h of exposure by fumigation in concentrations up to 400 µL L(-1) of air. Based on GC-MS analysis, 20 compounds were identified in the essential oil of P. pseudocaryophyllus leaves, being chavibetol (38.14%), methyl eugenol (11.35%), and terpinolene (9.17%) as the major constituents. Essential oil from P. pseudocaryophyllus leaves is an interesting source of compounds with grain-protectant properties and should be analyzed in future studies aiming to develop new bioinsecticides to use in the IPM of stored grains.