Correction of glycemic and oxidative stress sensors in streptozotocin induced diabetic rat model: Impact of dose dependent study of n-butanol solvent fraction of hydro-methanol extract of banana flower (Musa balbisiana).

The experiment focused the most effective dose out of 2.5, 5, and 10 mg/100 g body weight/day for 4 weeks of n-butanol fraction from the pretested promising hydro-methanol extract of Musa balbisiana flower to manage the streptozotocin-induced diabetes in rat and to minimize the bioburden of the body by excluding the inactive components from the said extract. Fraction treatment exhibited an outstanding recovery in fasting blood glucose, glycated hemoglobin, serum insulin levels, carbohydrate metabolic, antioxidant enzymes activities, expression of their marker genes, and apoptotic gene in diabetic rat. Flow-cytometric study of pancreatic ß-cell population was significantly recovered in fraction-treated groups which was also supported by histological study. The moderate dose, that is, 5 mg dose showed maximum efficacy in respect to other doses. The high dose, that is, 10 mg dose is not significantly effective than 5 mg dose may be due to the desensitization of the target organ's orphan receptor(s). PRACTICAL APPLICATIONS: The work includes the role of M. balbisiana on diabetes management. Result focused that M. balbisiana flower can able to rectify the diabetic condition and recovered the ß cell population. M. balbisiana flower is a very common vegetable to people, use mostly in their daily meal. The experiment showed positive results to cure diabetes in the long-term. Moreover, this study is potential for drug development using nutraceutical(s) isolation. The pharmaceutical companies may develop an antidiabetic drug from the concerned plant part after successful clinical trial.

Effect of lycopene on testicular androgenic and anti-oxidative enzymes in cyproterone acetate-induced male infertile Wistar strain albino rats: An in vitro study.

Incidents of male infertility are mushrooming worldwide. Oxidative stress plays a prime role for its onset. Considering this background, the study was designed to focus the direct role of lycopene on cyproterone acetate (CPA) induced testicular hypofunction in rat. Four groups have been considered including the vehicle-treated control, lycopene-treated control, CPA-treated and CPA+ lycopene-treated groups. Androgenic, antioxidant and toxicity profiles were assessed. Results focused a nonsignificant (p > .05) difference in recovery of testicular Δ5 , 3ß-hydroxysteroid dehydrogenase (HSD), 17ß-HSD after direct exposure of lycopene compared to the CPA-treated group. On other side, lycopene exposure to the testicular tissue of CPA-treated rat (CPA+ lycopene-treated) exhibited a significant (p < .05, p < .001) rectification in testicular catalase, superoxide dismutase, peroxidase, glutathione-S-transferase activities towards the vehicle- and lycopene-treated control groups. Toxicity profile also showed a significant (p < .001) recovery in CPA-treated group after direct exposure of lycopene towards the vehicle- and lycopene-treated control groups. So, it can be concluded that direct exposure of lycopene may rectify the CPA-induced testicular hypofunction either by its free radical-quenching ability or by stimulating antioxidant enzyme activity without modulating androgenic key enzyme directly.

Possible Antidiabetic and Antioxidative Activity of Hydro-Methanolic Extract of Musa balbisiana (Colla) Flower in Streptozotocin-Induced Diabetic Male Albino Wistar Strain Rat: A Genomic Approach.

The aim of the investigation was to search out the possible corrective effect of hydro-methanolic extract:: 3:2 of Musa balbisiana flower on streptozotocin (STZ)-induced diabetic male rat. In this concern, glycemic profile, oxidative stress profile, lipid profile, and toxicity profile were studied where a genomic approach has been taken to explain the mechanism of action of the said extract for such recovery. Such enzyme kinetics domain and genomic domain of concerned profile were not covered till now to explore the mechanism of diabetes management by this plant part. As hexokinase is one of the important enzymes of glycolysis and glycogenesis, and GLUT-4 an important transporter of glucose in skeletal muscle, liver and adipose tissue these genes have been selected here. For focusing the status of apoptosis in hepatic tissue, an important organ for carbohydrate metabolism, Bax and Bcl-2 gene expression were included, which are the novelty of this study. The hydro-methanolic extract was administered orally at the dose of 10 mg/100 g body weight for 28 days to diabetic rats. Abovementioned extract exhibited a significant recovery in parameters like fasting blood glucose; serum insulin; glycated hemoglobin; antioxidative enzymes in hepatic and renal tissue; and carbohydrate metabolic enzymes in hepatic and skeleto-muscular tissue along with proappoptotic gene Bax and antiappoptotic gene Bcl-2, glycemic genes like Hex-I, and GLUT-4 in hepatic tissue of STZ-induced diabetic rat. This investigation demonstrated the potentiality of hydro-methanolic extract (3:2) of M. balbisiana flower for correction of diabetes and diabetes-induced oxidative stress.