The impacts of Wolbachia and the microbiome on mate choice in Drosophila melanogaster.

Symbionts and parasites can manipulate their hosts' reproduction to their own benefit, profoundly influencing patterns of mate choice and evolution of the host population. Wolbachia is one of the most widespread symbionts among arthropods, and one that alters its hosts' reproduction in diverse and dramatic ways. While we are beginning to appreciate how Wolbachia's extreme manipulations of host reproduction can influence species diversification and reproductive isolation, we understand little about how symbionts and Wolbachia, in particular, may affect intrapopulation processes of mate choice. We hypothesized that the maternally transmitted Wolbachia would increase the attractiveness of its female hosts to further its own spread. We therefore tested the effects of Wolbachia removal and microbiome disruption on female attractiveness and male mate choice among ten isofemale lines of Drosophila melanogaster. We found variable effects of general microbiome disruption on female attractiveness, with indications that bacteria interact with hosts in a line-specific manner to affect female attractiveness. However, we found no evidence that Wolbachia influence female attractiveness or male mate choice among these lines. Although the endosymbiont Wolbachia can greatly alter the reproduction of their hosts in many species, there is no indication that they alter mate choice behaviours in D. melanogaster.

Divergent patterns of diversification in courtship and genitalic characters of Timema walking-sticks.

Understanding the patterns of diversification in sexual traits and the selection underlying such diversification represents a major unresolved question in evolutionary biology. We examined the phylogenetic diversification for courtship and external genitalic characters across ten species of Timema walking-sticks, to infer the tempos and modes of character change in these sexual traits and to draw inferences regarding the selective pressures underlying speciation and diversification in this clade. Rates of inferred change in male courtship behaviours were proportional to speciation events, but male external genitalic structures showed a pattern of continuous change across evolutionary time, with divergence proportional to branch lengths. These findings suggest that diversification of courtship behaviour is mediated by processes that occur in association with speciation, whereas diversification of genitalia occurs more or less continuously, most likely driven by forces of sexual selection.

The genetic architecture of insect courtship behavior and premating isolation.

The genetic architecture underlying reproductively isolating traits may have substantial impacts on the likelihood and pace of speciation. Recent studies of a key premating barrier, courtship, provide sufficient data to assess the degree to which behaviorally isolating traits are controlled by many or few loci, and help to investigate whether the same loci underlie both intraspecific and interspecific behavioral differences. Of the behavioral courtship traits examined, 69% (25 of 36) were found to be mediated by few loci of relatively large effect. This apparent prevalence of major loci suggests that changes in courtship behavior may often evolve quickly, which in turn may drive rapid speciation through premating isolation. Although both intraspecific and interspecific courtship differences are commonly controlled by major loci, intraspecific and interspecific differences usually involve different loci or traits. This finding provides evidence that different sets of processes and genetic changes characterize microevolutionary change in courtship-related traits, in contrast to change during speciation.