Acidic Skin Care Promotes Cutaneous Microbiome Recovery and Skin Physiology in an Acute Stratum Corneum Stress Model.

INTRODUCTION: Skin microbiome and skin physiology are important indicators of the epidermal homeostasis status. Stress models can reveal pathological conditions and modulating effects. Here we investigated the cutaneous microbiome in relation to skin physiology after mild tape stripping (TS) without treatment compared to two cosmetic leave-on lotions (pH 5.5 vs. pH 9.3) in 25 healthy volunteers. METHODS: The microbiome was analyzed by 16S-rRNA-gene amplicon sequencing and put in relation to the following skin physiology parameter: epidermal barrier function (TEWA-Meter TM300), stratum corneum hydration (Corneometer CM 825), surface pH (pH-Meter), and skin erythema (Mexameter). RESULTS: TS reduced the alpha diversity with a recovery over 7 days without treatment. Both lotions significantly accelerated the recovery of the alpha diversity already after 2 days with a slightly higher rate for the acidic lotion. After TS, the relative abundance of Proteobacteria was increased, whereas Actinobacteria were reduced. The relative abundances of typical skin-associated genera were reduced after TS. Taxa compositions returned to normal levels after 7 days in all treatment groups. An accelerated normalization could be observed with both lotions already after 2 days. A significant difference in skin pH was observed on day 2 and day 7 with an increased pH for the alkaline lotion. Both lotions induced an increase in stratum corneum hydration. CONCLUSION: The study proved the suitability of an experimental stress model in the assessment of skin surface microbiome in relation to skin physiology. Stratum corneum hydration increased significantly with both lotions already at day 2. Microbiome parameters (alpha diversity, mean relative taxa, abundance of selected genera) normalized over 2-7 days. The following mechanisms could be responsible for the accelerated normalization of the microbiome: (a) optimized hydration during the recovery phase, (b) the composition of the lotion, (c) the induced repair mechanism. Thus, the formulation has a positive effect on the stratum corneum hydration and subsequently on cutaneous microbiome and skin physiology. Furthermore, this eventually has implications on the modulation of exogenous stress-induced epidermal alterations.

A rapid, accurate, and facile method to quantify the antioxidative capacity of topical formulations.

BACKGROUND/AIMS: Various methodologies have been developed to quantify antioxidant activity. A simple, rapid and accurate method is demanded. This study examined the antioxidative status of a pH balanced vitamin E containing formulation versus its vehicle control utilizing a photochemiluminescence device. METHODS/RESULTS: A pH balanced 5% Vitamin E containing formulation and its vehicle control were tested. The quantity of antioxidant capacity for the pH balanced vitamin E formulation and its vehicle control were 2.28 +/- 0.05 and 0.16 +/- 0.03, respectively. The pH balanced vitamin E formulation showed a significant (P < 0.001) higher antioxidant capacity compared to its vehicle control. CONCLUSIONS: This method not only provides quantitative data, but also is rapid, accurate, and facile in performance. The in vitro data obtained in this study require validation by in vivo studies to properly place them in context to alternate methods.