Evaluation of influence of milk urea nitrogen on reproductive performance in smallholder dairy farms.

OBJECTIVE: The aim of this study was to determine the relationship between milk urea nitrogen (MUN) and reproductive performance in dairy cows in western Thailand. DESIGN: All cows calving from November 2014 to April 2015 were included in the study, a total of 486 cows from 47 farms. Each cow had milk constituents and MUN tested monthly up to confirmed conception or until the 8th month after parturition. Each farm had a dietary assessment completed. Cox proportional hazard models with shared frailty were used to determine associations of conception rate. RESULTS: Cows became pregnant increasingly quickly over time, except during 100-150 days of lactation. A change in MUN from 12.5 to 13.5 mg/dL on the closet day to breeding was associated with a 5% decrease in conception. Milk protein was negatively associated with hazard of conception, whereas milk lactose and dietary protein:energy ratio had positive associations with conception rate. Breeding season was also significant; the highest conception rate was observed in cows inseminated during winter, whereas insemination during the humid rainy season resulted in the lowest conception rates. The farm random effect in the model was strongly significant. CONCLUSION: Detrimental effects of higher MUN on rate of conception were identified. The rate of conception was positively associated with protein:energy ratio in the study. Therefore, good nutritional management leading to positive energy balance should benefit conception rates.

Milk urea-nitrogen negatively affected first-service breeding success in commercial dairy cows in Prince Edward Island, Canada.

Our objective of this study was to investigate the relationship between milk urea-nitrogen concentrations ([MUN]) and first-service breeding success (FSBS) in a large number of commercial dairy herds, using various timings on [MUN]. All commercial dairy herds in Prince Edward Island on monthly milk testing (n=198) formed the sampling frame. Milk components, [MUN], 24-h milk production, and breeding data for all cows from these farms were gathered electronically from a central database. A first service between 1 June 1999 and 31 May 2000 was classified successful (FSBS=1) if it was the cow's last service and she calved 270-290 d later. Mixed logistic-regression modeling was used to determine the association between FSBS (the outcome variable) and the [MUN] closest to first service, controlling for other possible confounders and clustering effects of cows within the study herds. The final dataset included 2787 successful and 3015 unsuccessful first services. A change in [MUN] on the test closest to first service from 10 to 20 mg/dL was associated with a 13.9% reduction in the odds of FSBS (controlled for parity, milk production and days in milk).

Bulk tank milk urea nitrogen: seasonal patterns and relationship to individual cow milk urea nitrogen values.

The objectives of this study were: 1) to determine if bulk tank milk urea nitrogen (BTMUN) and whole herd weighted average of the individual cow MUN levels (WHMUN) were equivalent measurements of herd MUN status; and 2) to determine the seasonal variation in BTMUN concentrations in Prince Edward Island (PEI) dairy herds. For BTMUN-WHMUN correlation testing, bulk tank milk samples from 176 herds were tested for MUN once every 1 to 2 wk between September 1999 and August 2002, as part of routine BTM testing for milk components. During this 3-year period, all herds had all milking cows tested for MUN once a month at the same lab. The WHMUN levels (weighted for milk production) were calculated for each month, and were compared to BTMUN levels using a concordance correlation coefficient (CCC) and a graphic procedure. Tests were only compared if they occurred on the same date, producing a final dataset of 669 comparisons. The BTMUN had good (but not perfect) correlation with WHMUN (CCC = 0.91). This high reliability extended to both the pasture and non-pasture seasons, various milk sampling protocols, and all herd sizes seen in PEI. For evaluating the seasonal variation of BTMUN, the 3 y worth of data (24 803 observations) were divided into 15 seasonal categories, 5 seasons per year (early, mid, and late pasture, and early and late stable). Using linear mixed modelling, significantly (P < 0.05) higher BTMUN values were found during the mid and late pasture seasons of 2000, likely because the precipitation was unusually high during this period, enhancing pasture growth.

The effect of non-nutritional factors on milk urea nitrogen levels in dairy cows in Prince Edward Island, Canada.

We determined the effects of non-nutritional factors such as breed, parity, days in milk (DIM), milk production, milk quality and milk components on milk urea nitrogen (MUN) concentration. A total of 177 dairy farms in Prince Edward Island containing 10,688 lactating dairy cows participated in the project. Individual-cow milk samples (n=68,158) were collected monthly from July 1999 to June 2000 from each farm. MUN was measured using a Fossomatic 4000 Milkoscan Analyzer at the PEI Milk Quality Laboratory. Descriptive statistics for MUN, parity, DIM, and test-day milk yield, fat and protein were calculated. Mixed linear-regression models were used; "cow" and "herd" were included as random effects to control for the effect of clustering of MUN test dates within cow, and clustering of cows within herd, respectively. The MUN was lower during the first month of lactation, peaked at 4 months of lactation, and decreased later in lactation. A positive relationship existed between MUN concentration and milk yield, while negative relationships with milk protein% and linear score were observed. A quadratic relationship existed between milk fat% and MUN concentration, with higher MUN occurring at mid-range fat percentages. The variation at the herd and cow levels in the model were 19.7 and 19.0%, respectively; while the variation at the test date level was 61.3%. The non-nutritional factors studied explained 13.3% of the variation in MUN.

Evaluation of the reliability and repeatability of automated milk urea nitrogen testing.

The purpose of this study was to evaluate the reliability (precision and accuracy), and repeatability of an infrared method of determining milk urea nitrogen. The reference method used for the reliability assessment was a wet-chemistry, enzymatic determination of milk urea nitrogen. Reliability and repeatability, as measured by concordance correlation coefficients, were 0.97 and 0.99, respectively.