Integrating biosorption and machine learning for efficient remazol red removal by algae-bacteria co-culture and comparative analysis of predicted models.

This research investigates into the efficacy of algae and algae-bacteria symbiosis (ABS) in efficiently decolorizing Remazol Red 5B, a prevalent dye pollutant. The investigation encompasses an exploration of the biosorption isotherm and kinetics governing the dye removal process. Additionally, various machine learning models are employed to predict the efficiency of dye removal within a co-culture system. The results demonstrate that both Desmodesmus abundans and a composite of Desmodesmus abundans and Rhodococcus pyridinivorans exhibit significant dye removal percentages of 75 ± 1% and 78 ± 1%, respectively, after 40 min. The biosorption isotherm analysis reveals a significant interaction between the adsorbate and the biosorbent, and it indicates that the Temkin model best matches the experimental data. Moreover, the Langmuir model indicates a relatively high biosorption capacity, further highlighting the potential of the algae-bacteria composite as an efficient adsorbent. Decision Trees, Random Forest, Support Vector Regression, and Artificial Neural Networks are evaluated for predicting dye removal efficiency. The Random Forest model emerges as the most accurate, exhibiting an R2 value of 0.98, while Support Vector Regression and Artificial Neural Networks also demonstrate robust predictive capabilities. This study contributes to the advancement of sustainable dye removal strategies and encourages future exploration of hybrid approaches to further enhance predictive accuracy and efficiency in wastewater treatment processes.

Bioprocess optimization for enhanced xylitol synthesis by new isolate Meyerozyma caribbica CP02 using rice straw.

The present work models the fermentation process parameters of the newly isolated, Meyerozyma caribbica CP02 for enhanced xylitol production and its fermentability study on rice straw hydrolysate. The study examined the impact of each of the process variables by one variable at a time optimization followed by statistical validation. Temperature of 32 °C, pH of 3.5, agitation of 200 rpm, 1.5% (v/v) inoculum, 80 gL-1 initial xylose was optimized. Subsequently, a sequential two-stage agitation approach was adopted for fermentation. At these optimized conditions, xylitol yield of 0.77 gg-1 and 0.64 gg-1 was achieved using media containing commercial and rice straw derived xylose, respectively. For scale up, in 3L batch bioreactor, the highest xylitol yield (0.63 gg-1) was attained at 72 h with rice straw hydrolysate media containing initial xylose (59.48 ± 0.82 gL-1) along with inhibitors (1.55 ± 0.10 gL-1 aliphatic acids, 0.0.048 ± 0.11 gL-1 furans, 0.64 ± 0.23 gL-1 total phenols). The results imply that even under circumstances characterized by an acidic pH and elevated initial xylose level, M. caribbica CP02, as an isolate, displays robustness and shows favorable fermentability of rice straw hydrolysate. Therefore, isolate CP02 has potential to be used in bio-refineries for high yield xylitol production with minimal hydrolysate processing requirements.

Algal carbohydrate polymers: Catalytic innovations for sustainable development.

Algal polysaccharides, harnessed for their catalytic potential, embody a compelling narrative in sustainable chemistry. This review explores the complex domains of algal carbohydrate-based catalysis, revealing its diverse trajectory. Starting with algal polysaccharide synthesis and characterization methods as catalysts, the investigation includes sophisticated techniques like NMR spectroscopy that provide deep insights into the structural variety of these materials. Algal polysaccharides undergo various preparation and modification techniques to enhance their catalytic activity such as immobilization. Homogeneous catalysis, revealing its significance in practical applications like crafting organic compounds and facilitating chemical transformations. Recent studies showcase how algal-derived catalysts prove to be remarkably versatile, showcasing their ability to customise reactions for specific substances. Heterogeneous catalysis, it highlights the significance of immobilization techniques, playing a central role in ensuring stability and the ability to reuse catalysts. The practical applications of heterogeneous algal catalysts in converting biomass and breaking down contaminants, supported by real-life case studies, emphasize their effectiveness. In sustainable chemistry, algal polysaccharides emerge as compelling catalysts, offering a unique intersection of eco-friendliness, structural diversity, and versatile catalytic properties. Tackling challenges such as dealing with complex structural variations, ensuring the stability of the catalyst, and addressing economic considerations calls for out-of-the-box and inventive solutions. Embracing the circular economy mindset not only assures sustainable catalyst design but also promotes efficient recycling practices. The use of algal carbohydrates in catalysis stands out as a source of optimism, paving the way for a future where chemistry aligns seamlessly with nature, guiding us toward a sustainable, eco-friendly, and thriving tomorrow. This review encapsulates-structural insights, catalytic applications, challenges, and future perspectives-invoking a call for collective commitment to catalyze a sustainable scientific revolution.

A review on cutinases enzyme in degradation of microplastics.

From the surface of the earth to the depths of the ocean, microplastics are a hazard for both aquatic and terrestrial habitats. Due to their small size and vast expanse, they can further integrate into living things. The fate of microplastics in the environment depends upon the biotic components such as microorganisms which have potential enzymes to degrade the microplastics. As a result, scientists are interested in using microorganisms like bacteria, fungi, and others to remediate microplastic. These microorganisms release the cutinase enzyme, which is associated with the enzymatic breakdown of microplastics and plastic films. Yet, numerous varieties of microplastics exist in the environment and their contaminants act as a significant challenge in degrading microplastics. The review discusses the cutinases enzyme degradation strategies and potential answers to deal with existing and newly generated microplastic waste - polyethylene (PE), polyethylene terephthalate (PET), poly-ε-caprolactone (PCL), polyurethanes (PU), and polybutylene succinate (PBS), along with their degradation pathways. The potential of cutinase enzymes from various microorganisms can effectively act to remediate the global problem of microplastic pollution.

Microplastics/nanoplastics released from facemasks as contaminants of emerging concern.

Facemasks have become a global medical necessity and are a key preventive measure against COVID-19. Typically, facemasks (FMs) are fabricated from non-renewable polymers, particularly polypropylene (PP) and polyethylene (PE), which release secondary microplastic (MPs) due to the chemical, physical, and biological processes. In light of the widespread usage and improper disposal of single-use facemasks, there is concern about their environmental impact since they contribute to plastic pollution during and after pandemics. The repercussions of this have led to millions of tons of plastic waste being dumped into the environment. Due to lack of awareness and improper disposal, the occurrence of micro/nanoplastics released from facemasks in wastewater treatment plants and landfills poses a concern. Infiltration of wastewater treatment processes by micro/nanoplastics at various levels can be problematic because of their chemical nature and broad but small size. Thus, operational and process stability issues can arise during wastewater treatment processes. In addition, landfilling and illegal waste disposal are being used to dispose of potentially infectious COVID-19 waste, leading to an environmental threat to animal and human health and exacerbating plastic pollution. This paper reviews the fate of facemasks in the environment and the repercussions of improper waste management of facemasks in wastewater treatment plants, landfills, and ultimately the environment.

Prospects of chitinase in sustainable farming and modern biotechnology: an update on recent progress and challenges.

Chitinases are multifunctional biocatalysts for the pest control and useful in modern biotechnology and pharmaceutical industries. Chemical-based fungicides and insecticides have caused more severe effects on environment and human health. Many pathogenic fungal species and insects became resistant to the chemical pesticides. The resistant fungi emerged as a multidrug resistant also and less susceptible insects are not possible to control adequately. Chitinases have an immense potential to be exploited as a biopesticide against fungi and insects. The direct use of chitinase in liquid formulation or whole microbial enzyme producing cells, both act as antagonistically against the pests. Chitinase can disintegrate the fungal cell wall and insect integument that holds the chitin as a vital structural component. Moreover, chitinase is applied for the synthesis of pharmaceutically important chitooligosaccharides. Chitinase producing microbes have the huge potential to utilize against the waste management of sea food remains like shells of crustaceans. Chitinase is valuable for the synthesis of protoplasts from industrially important fungi, further it act as the biocontrol agent of malaria and dengue fever causing larvae of mosquitoes. Chitinases also have been successfully used in wine and single cell protein producing industries. Present review is illustrating the updated information on the state of the art of different applications of chitinases in agriculture and biotechnology industry. It also bestows the understanding to the readers about the areas of extensively studied and the field where there is still much left to be done.

Laccase mediated delignification of wasted and non-food agricultural biomass: Recent developments and challenges.

Utilization of microbial laccases is considered as the cleaner and target specific biocatalytic mechanism for the recovery of cellulose and hemicelluloses from nonfood and wasted agricultural, lignocellulosic biomass (LCB). The extent of lignin removal by laccase depends on the biochemical composition of biomass and the redox potential (E0) of the biocatalyst. Intensive research efforts are going on all over the world for the recognition of appropriate and easily available agricultural lignocellulosic feedstocks to exploit maximally for the production of value-added bioproducts and biofuels. In such circumstances, laccase can play a major role as a leading biocatalyst and potent substitute for chemical based deconstruction of the lignocellulosic materials. The limited commercialization of laccase at an industrial scale has been feasible due to its full working efficiency mostly expressed in the presence of cost intensive redox mediators only. Although, recently there are some reports that came on the mediator free biocatalysis of enzyme but still not considerably explored and neither understood in depth. The present review will address the various research gaps and shortcomings that acted as the big hurdles before the complete exploitation of laccases at an industrial scale. Further, this article also reveals insights on different microbial laccases and their diverse functional environmental conditions that affect the deconstruction process of LCB.

An alternative approach towards nitrification and bioremediation of wastewater from aquaponics using biofilm-based bioreactors: A review.

Aquaponics combines the advantages of aquaculture and hydroponics as it suits the urban environment where a lack of agricultural land and water resources is observed. It is an ecologically sound system that completely reuses its system waste as plant fertilizer. It offers sustainable water savings, making it a supreme technology for food production. The two major processes that hold the system together are nitrification and denitrification. The remains of fish in form of ammonia reach the bio filters where it is converted into nitrite and further into nitrate in presence of nitrifying and denitrifying bacteria. Nitrate eventually is taken up by the plants. However, even after the uptake from the flow stream, the effluent contains remaining ammonium and nitrates, which cannot be directly released into the environment. In this review it is suggested how integrating the biofilm-based bioreactors in addition to aquaculture and hydroponics eliminates the possibility of remains of total ammonia nitrogen [TAN] contents, leading to bioremediation of effluent water from the system. Effluent water after releasing from a bioreactor can be reused in an aquaculture system, conditions provided in these bioreactors promote the growth of required bacteria and encourages the mutual development of plants and fishes and eventually leading to bioremediation of wastewater from aquaponics.

Phytoremediation of radioactive elements, possibilities and challenges: special focus on agricultural aspects.

The radioactive contamination has been reported frequently from agricultural lands and ground water. The main reason behind the radioactive pollution is unprotected mining of radioactive elements, unsafe discard of nuclear industrial waste, military applications, dumping of medically used radioisotopes, globally situated (>400) nuclear power plants and use of phosphate fertilizers in farming. Radionuclides are well known potent carcinogens that may cause the various types of cancers to human and animals due to the long exposure to radioactive contaminated sites. To get rid of from the radioactive pollution there is a need of practically successful and cost effective bioremediation technologies that should able to decontaminate the effected lands and water to benefit the mankind. Microbial and phytoremediation are well studied methods for decreasing or gradually eliminating the radioactive contaminants. In this review, we discussed the different strategies of microbial and phytoremediation of radionuclides and recent advancements, that can play the major role in bioremediation of soil and water.

Different remediation technologies based on physical (precipitation, extraction and membrane separation technologies) and chemical (chemical extraction and leaching, hydrolysis, etc.) methods to remediate the radioactive compounds from soils and water are being developed and evaluated. Most of these technologies are cost intensive and only applicable to little contaminated sites. On the other hand phytoremediation and microbial bioremediation are scientifically proven for applying at large scale and economical. Phytoremediation is one of the bioengineering treatments in which terrestrial and aquatic plants have been successfully used for cleaning the radioactive pollutants from diverse environments. Present review article is a updating the recent developments came in the different bioremediation methods. Moreover aim of this manuscript is also emerging the research gaps and identified the future research frontiers to unlock the complexity of phyto and microbial remediation advancements. Although several plants and numerous bacteria and fungi have been identified as the potential radioactive accumulator but their complete mechanism of bioremediation is still unknown. Present article will help the researchers to understand the process of bioremediation of radionuclides in more depths and will aware about the requirements of the coming future.HighlightsPhyto and microbial bioremediation of radioactive elements, possibilities and challenges.Causes of radioactive contamination in soil and water.Nanophytoremediation is an advanced technology of phytoremediation.Drawbacks of phytoremediation.

Laccase-assisted degradation of emerging recalcitrant compounds - A review.

The main objective of this review is to provide up to date, brief, irrefutable, organized data on the conducted experiments on a range of emerging recalcitrant compounds such as Diclofenac (DCF), Chlorophenols (CPs), tetracycline (TCs), Triclosan (TCS), Bisphenol A (BPA) and Carbamazepine (CBZ). These compounds were selected from the categories of pharmaceutical contaminants (PCs), endocrine disruptors (EDs) and personal care products (PCPs) on the basis of their toxicity and concentration retained in the environment. In this context, detailed mechanism of laccase mediated degradation has been conversed that laccase assisted degradation occurs by one electron oxidation involving redox potential as underlying element of the process. Further, converging towards biotechnology, laccase immobilization increased removal efficiency, storage and reusability through various experimentally conducted studies. Laccase is being considered noteworthy as mediators facilitate laccase in oxidation of non-phenolic compounds and thereby increasing its substrate range which is being discussed in further in the review. The laccase assisted degradation mechanism of each compound has been elucidated but further studies to undercover proper degradation mechanisms needs to be performed.

Meta-analysis of in-vitro cytotoxicity evaluation studies of zinc oxide nanoparticles: Paving way for safer innovations.

Nano-based products have shown their daunting presence in several sectors. Among them, Zinc Oxide (ZnO) nanoparticles wangled the reputation of providing "next-generation solutions" and are being utilized in plethora of products. Their widespread application has led to increased exposure of these particles, raising concerns regarding toxicological repercussions to the human health and environment. The diversity, complexity, and heterogeneity in the available literature, along with correlation of befitting attributes, makes it challenging to develop one systematic framework to predict this toxicity. The present study aims at developing predictive modelling framework to tap the prospective features responsible for causing cytotoxicity in-vitro on exposure to ZnO nanoparticles. Rigorous approach was used to mine the information from complete body of evidence published to date. The attributes, features and experimental conditions were systematically extracted to unmask the effect of varied features. 1240 data points from 76 publications were obtained, containing 14 qualitative and quantitative attributes, including physiochemical properties of nanoparticles, cell culture and experimental parameters to perform meta-analysis. For the first time, the efforts were made to investigate the degree of significance of attributes accountable for causing cytotoxicity on exposure to ZnO nanoparticles. We show that in-vitro cytotoxicity is closely related with dose concentration of nanoparticles, followed by exposure time, disease state of the cell line and size of these nanoparticles among other attributes.

The eco-friendly approach of cocktail enzyme in agricultural waste treatment: A comprehensive review.

Agricultural development over the past decade has majorly contributed to the world's bioeconomy, but is the rise in agricultural activities just resulting in the best? Farming, food processing, livestock handling and other agro-based actions show an incremental rise in environmental deterioration by generating millions of tonnes of organic and inorganic solid waste across the globe. Incautious waste handling practices (incineration and landfilling) is resulting in greenhouse gas emissions, land pollution, groundwater contamination, soil erosion and chronic health hazards. Lately the concept of bioconversion has gained importance in valorising agro-waste (lignocellulosic biomasses) into value added products like biofuels, biogas, single cell proteins and biochar to effectively control waste and reduce the dependency on non-renewable feedstocks (fossil fuels). Biomass hydrolysis via enzymes is improved in terms of cost, efficiency, catalysis, stability and specificity by enrolling the use of enzyme cocktails to synergistically degrade lignocellulose into monomeric sugars and further into valued products. Enzyme blends like that of Xylanase + Pectinase + Cellulase shows 76.5% fermentation within 30 h by using banana peel as substrate for biofuel production. Other sectors like paper industries have also explored the use of enzyme blends of Xylanase + Pectinase + α-amylase + Protease+ lipase for bio-bleaching showing reduction in 50% chemical usage and 19.5% kappa number with adjacent increase in tensile strength by 23.55%. The scope of the present review is to highlight the technicalities of the concepts mentioned above, include qualitative data from different relatable studies and prove how the use of enzyme cocktails is an eco-friendly approach towards agro-waste management.

Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC).

This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by green synthesis method. Both free and immobilized xylanase (Xy-Cu-BTC) were able to cause the bioconversion of xylan (87.4% yield) into XOS. Predominant production of xylotetrose (X4) and xylopentose (X5) was observed for both the methods. Percentage XOS conversion for free enzyme (Xy) was found to be 4.1% X4 and 60.57% X5 whereas these values increased in case of immobilized system where 11.8% X4 and 64.2% X5 were produced. Xylose production was minute in case of immobilized xylanase 0.88% which makes it a better method for XOS production free from xylose interference. Xy-Cu-BTC MOF can hence be used as an attractive alternative for pure XOS production.

An Elucidative Review to Analytically Sieve the Viability of Nanomedicine Market.

The advent of the twenty-first century marked a paradigm shift in the healthcare sector with coming of automated, sensitive, targeted medicines and technologies having diagnostic, prophylactic and therapeutic effects. Nanomedicines also attained wide acclamation in their initial years, but the transformation from being the proof of concept to successfully marketed products seems very daunting. Although the reason for this may be attributed to slow but incremental character of many present-day technologies, the review asserts that there are other significant facets that may purvey a thorough explanation of this scenario. The article elaborately discusses the hurdles hindering clinical translation of nanomedicines including scale-up challenges, in vitro in vivo cascade of toxicology assays, along with unrefined manufacturing guidelines, inadequate regulatory approvals, competitive conventional market, etc., leading to hesitant investments by pharmaceutical giants. The paper also explores the economic viability of nanobiotechnology sector through an empirical investigation of the revenue data of various pharmaceutical industries manufacturing nano-based drugs, which indicates minor commercial importance of these medicines. We also laid down a comprehensive set of recommendations to smoothen the translational pathway of nanomedicines from an idea to reality, efface the consumer distrust and push boundaries for development and launching of safe, efficient and commercially successful products. Graphical abstract.

A multifaceted enzyme conspicuous in fruit juice clarification: An elaborate review on xylanase.

Xylanase enzyme has been classified as an enzyme belonging to the glycoside hydrolase family. The catalytic action of xylanase is focused on the degradation of xylan, a substrate for this enzyme comprising of a complex arrangement of monosaccharides interlinked with the help of ester and glycosidic bonds. Xylan represents the second most profuse renewable polysaccharide present on earth. Breakage of the ß- 1, 4-glycoside linkage in the xylan polymer is what makes xylanase enzyme an important biocatalyst favoring various applications including treatment of pulp for improving paper quality, improvement of bread quality, treatment of lignocelluloses waste, production of xylose sugar and production of biological fuels. Most recently, xylanase has been exploited in the food industry for the purpose of fruit juice clarification. Turbidity caused by the colloidal polysaccharides present in the freshly squeezed fruit juice poses a setback to the fruit juice industry since the commercial product must be clear and free of excess polysaccharides to improve juice quality and storage life. This review gives an overview of the recent advancements made in regards to xylanase enzyme being used commercially with main focus on its role in fruit juice clarification.

Recent advances in the applications of nano-agrochemicals for sustainable agricultural development.

Modern agricultural practices have triggered the process of agricultural pollution. This process can cause the degradation of eco-systems, land, and environment owing to the modern-day by-products of agriculture. The substantial use of chemical fertilizers, pesticides, and, contaminated water for irrigation cause further damage to agriculture. The current scenario of the agriculture and food sector has therefore become unsustainable. Nanotechnology has provided innovative and resourceful frontiers to the agriculture sector by contributing practical applications in conventional agricultural ways and practices. There is a large possibility that agri-nanotechnology can have a significant impact on the sustainable agriculture and crop growth. Recent research has shown the potential of nanotechnology in improving the agriculture sector by enhancing the efficiency of agricultural inputs and providing solutions to agricultural problems for improving food productivity and security. The prospective use of nanoscale agrochemicals such as nanofertilizers, nanopesticides, nanosensors, and nanoformulations in agriculture has transformed traditional agro-practices, making them more sustainable and efficient. However, the application of these nano-products in real field situations raises concern about nanomaterial safety, exposure levels, and toxicological repercussions to the environment and human health. The present review gives an insight into recent advancements in nanotechnology-based agrochemicals that have revolutionized the agriculture sector. Further, the implementation barriers related to the nanomaterial use in agriculture, their commercialization potential, and the need for policy regulations to assess possible nano-agricultural risks are also discussed.

A treatise on Organophosphate pesticide pollution: Current strategies and advancements in their environmental degradation and elimination.

Pesticides have been used in the field of agriculture ever since their role in protection of crops from pests which include four different categories namely insects, mites, rodents and animals has been identified. Organophosphate pesticides are one of the most extensively applied insecticides in the field of agriculture such that around 40% of all the pesticides that are produced and used commercially belong to this category. The main toxicological effect of these pesticides when exposed to a living being encompasses the irremediable inhibition of the acetylcholinesterase (AChE) enzyme which is involved in the neurotransmission of signals and hence its inhibition causes impairment of the respiratory tract and neuromuscular transmission. Apart from being used as a pesticide, organophosphates have also been applied as herbicides to some extent. The residues of these highly toxic chemicals have found route into the underground water system by seeping into the ground, in rivers where the agricultural run off water is disposed, and in the air when sprayed on the crops hence posing a threat to all the living strata exposed to these chemicals in various ways which are discussed further. Many significant studies have been carried out in order to evaluate the health risks associated with these pesticides which commonly include acute neurological disorders. This review emphasizes on the toxicological effects of organophosphate pesticides and the recent methods of detection that are used to identify trace amounts of organophosphate pesticides along with strategies which are used for their degradation.

Immobilization of keratinase on chitosan grafted-ß-cyclodextrin for the improvement of the enzyme properties and application of free keratinase in the textile industry.

Immobilization of enzymes is an effective and potential technique for improving the enzyme characteristics and plays an important role in reducing the final cost of enzymatic reactions. However, the method of enzyme immobilization should be easy, cost-effective and environment friendly when applicable at industrial scale. In present study, the successful biochemical characterization of free and immobilized keratinase was evaluated. The enzyme was effectively immobilized on chitosan and chitosan grafted-ß-cyclodextrin beads. Enzyme yield of immobilized biocatalyst on chitosan alone and chitosan-ß-CD-E was determined to be 90 and 93% respectively. Keratinase was able to act in highly alkaline conditions (optimum pH 11) both in free and immobilized form and showed maximum enzyme activity at 70 and 75 °C respectively. The free and immobilized enzyme exhibited remarkable thermo stability at 70 °C implying that it is capable for its usage in textile industry. The storage stability and reusability of the immobilized keratinase (chitosan-E and chitosan-ß-CD-E) was significantly enhanced, with 25 and 53.5% activity, respectively, retained at 4 °C after 30 days of storage. In the preliminary experiments it was found that free keratinase have the potential to improve the quality of woollen fabrics and suitable for application in textile industries.

Isolation and characterization of an alkali and thermostable laccase from a novel Alcaligenes faecalis and its application in decolorization of synthetic dyes.

A laccase producing new bacterial strain (Alcaligenes faecalis XF1) was isolated from green site of Chandigarh (India) by standard screening method. Nutrient broth medium containing 0.2 mM CuSO4 was used for the production of laccase. Maximum production (110 U/ml) was achieved after four days of incubation. The extracellular laccase from the medium was purified by simple salt precipitation and ion exchange technique to get 3.8 fold purified protein with 1637.8 U/mg specific activity. Purified laccase (named as LAC1*) revealed its optimum activity at pH 8.0 and 80 °C temperature, and displayed remarkable stability in the range of 70-90 °C and in the pH range (pH 7.0-9.0). The single bands on SDS-PAGE represents the purity of LAC1* with molecular weight of ∼71 kDa. The kinetic parameters for 2,6-DMP oxidation was Km, Vmax and kcat were 480 µM, 110 U/mL and 1375 s-1. Enzyme activity of the LAC1* was significantly enhanced by Cu2+, Mg2+, Mn2+, SDS and NaCl, and was slightly inhibited in the presence of conventional inhibitors like cysteine, EDTA and sodium azide. Extracellular nature and significant stability of LAC1* under extreme conditions of temperature, pH, heavy metals, halides and detergents confined its suitability for various biotechnological and industrial applications which required these qualities of laccase. So after recognizing all these properties the purified laccase was studied for its application in decolorization of industrial dyes.

Immobilization of mannanase on sodium alginate-grafted-ß-cyclodextrin: An easy and cost effective approach for the improvement of enzyme properties.

Partially purified ß-mannanase was immobilized on the modified matrix of sodium alginate-grafted-ß-cyclodextrin. The Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction characterization proved that ß-cyclodextrin (ß-CD) was successfully grafted with sodium alginate. After successful immobilization, yield of enzyme was found 91.5%, pH and temperature optima were increased, 6.0 to 7.0 and 50 °C to 55 °C respectively. Immobilized mannanase was able to reuse 15 times and retained its 70% activity, meanwhile the immobilized enzyme showed 60% activity after 30 days of storage at 4 °C. Immobilization also increased the thermostability and half-life of the enzyme when compared to the free mannanase. During the comparison of adsorption isotherm and kinetic models, Langmuir isotherm and pseudo-first order kinetics were observed to be the best fit model for the confirmation of immobilization.