Effect of GA3 and calcium on growth, biochemical, and fatty acid composition of linseed under chloride-dominated salinity.

The accumulation of salts in soil is an environmental threat affecting plant growth and crop yield. Linseed or flax is an ancient crop that has multifarious utilities in terms of industrial oil, textile fiber, and products. Salt susceptibility adversely affects linseed production, particularly to meet the growing demand for nutritional and nutraceutical products. In the present study, the ameliorative potential of gibberellic acid (GA3) and calcium (Ca2+) in mitigating the adverse effects of chloride-dominated salinity stress on the growth and physiological and biochemical processes in linseed was determined. Severe salinity treatment (10 dSm-1) resulted in stunted growth of tested linseed genotypes causing a significant reduction in biomass while proline content, phenol, H2O2, lipid peroxidation, and DPPH activity were increased in comparison to control. The exogenous application of 10-6 M GA3 and/or 10 mg CaCl2 kg-1 was found to mitigate the adverse effects of salinity stress. The mitigation was accomplished through the improvement of growth indicators, increased osmoprotectants such as proline and phenol content, stimulating DPPH activity, and reduction of H2O2 content and lipid peroxidation. The comparative evaluation of different saline treatments imposed individually and in combination with GA3 and Ca2+ revealed that combined GA3 and Ca2+ application exhibited synergistic effects and was most effective in mitigating the negative impacts of salt stress. The present study unravels the ameliorative role of GA3 and Ca2+ (individual or combined) in the physiologic-biochemical adaptive response of linseed plants grown under chloride-dominated salinity and thus aids in a better understanding of the underlying tolerance mechanisms of plants to withstand stress in saline environments.

Spermidine exogenous application mollifies reproductive stage heat stress ramifications in rice.

Introduction: Rice productivity is severely hampered by heat stress (HS) which induces oxidative stress in this crop. This oxidative stress can be alleviated using various exogenous chemicals, including spermidine (Spd). Therefore, the present study was carried out to characterize HS components and to elucidate the role of exogenous Spd application in rice at the flowering stage. Methods: Two contrasting rice genotypes, i.e. Nagina22 (N22) and Pusa Basmati-1121 (PB-1121) were placed in temperature tunnels and exposed to HS (38-43°C) with and without Spd (1.5 mM) foliar application during the heading stage till the end of the anthesis stage. Result: Heat stress induced the production of H2O2 and thiobarbituric acid reactive substances, which resulted in lower photosynthesis, spikelet sterility, and reduced grain yield. Interestingly, foliar application of Spd induced antioxidant enzyme activities and thus increased total antioxidant capacity resulting in higher photosynthesis, spikelet fertility, and improved grain yield under HS in both genotypes. Under HS with Spd, higher sugar content was recorded as compared to HS alone, which maintained the osmotic equilibrium in leaf and spikelets. Spd application initiated in vivo polyamine biosynthesis, which increased endogenous polyamine levels. Discussion: This study corroborates that the exogenous application of Spd is promising in induction of antioxidant defence and ameliorating HS tolerance in rice via improved photosynthesis and transpiration. Thereby, the study proposes the potential application of Spd to reduce HS in rice under current global warming scenario.