Seed priming with selenium improves growth and yield of quinoa plants suffering drought.

Drought stress is a worldwide threat to the productivity of crops, especially in arid and semi-arid zones of the world. In the present study, the effect of selenium (Se) seed priming on the yield of quinoa under normal and drought conditions was investigated. A pot trial was executed to enhance the drought tolerance in quinoa by Se seed priming (0, 3, 6, and 9 mg Se L-1). The plants were exposed to water stress at three different growth stages of quinoa, viz. multiple leaf, flowering, and seed filling. It was noticed that drought significantly affected the yield components of quinoa, however, Se priming improved the drought tolerance potential and yield of quinoa by maintaining the plant water status. Se priming significantly increased main panicle length (20.29%), main panicle weight (26.43%), and thousand grain weight (15.41%) as well as the gas exchange parameters (transpiration rate (29.74%), stomatal conductance (35.29%), and photosynthetic rate (28.79%), total phenolics (29.36%), leaf chlorophyll contents (35.97%), water relations (leaf relative water contents (14.55%), osmotic potential (10.32%), water potential (38.35%), and turgor potential (31.37%), and economic yield (35.99%) under drought stress. Moreover, Se priming markedly improved grain quality parameters i.e., phosphorus, potassium, and protein contents by 21.28%, 18.92%, and 15.04%, respectively. The principal component analysis connected the various study scales and showed the ability of physio-biochemical factors to describe yield fluctuations in response to Se seed priming under drought conditions. In conclusion, a drought at the seed-filling stage has a far more deleterious impact among other critical growth stages and seed priming with Se (6 mg L-1) was found more effective in alleviating the detrimental effects of drought on the grain yield of quinoa.

Clinical predictions of COVID-19 patients using deep stacking neural networks.

The coronavirus disease 2019 (COVID-19) pandemic, which emerged in late 2019, has caused millions of infections and fatalities globally, disrupting various aspects of human society, including socioeconomic, political, and educational systems. One of the key challenges during the COVID-19 pandemic is accurately predicting the clinical development and outcome of the infected patients. In response, scientists and medical professionals globally have mobilized to develop prognostic strategies such as risk scores, biomarkers, and machine learning models to predict the clinical course and outcomes of COVID-19 patients. In this contribution, we deployed a mathematical approach called matrix factorization feature selection to select the most relevant features from the anonymized laboratory biomarkers and demographic data of COVID-19 patients. Based on these features, developed a model that leverages the deep stacking neural network (DSNN) to aid in clinical care by predicting patients' mortality risk. To gauge the performance of our suggested model, performed a comparative analysis with principal component analysis plus support vector machine, deep learning, and random forest, achieving outstanding performances. The DSNN model outperformed all the other models in terms of area under the curve (96.0%), F1-score (98.1%), recall (98.5%), accuracy (99.0%), precision (97.7%), specificity (97.0%), and maximum probability of correction decision (93.4%). Our model outperforms the clinical predictive models regarding patient mortality risk and classification in the literature. Therefore, we conclude that our robust model can help healthcare professionals to manage COVID-19 patients more effectively. We expect that early prediction of COVID-19 patients and preventive interventions can reduce the mortality risk of patients.

Improving the genetic potential of okra (Abelmoschus esculentus L.) germplasm to tolerate salinity stress.

Okra (Abelmoschus esculentus L.) is the most consumed vegetable worldwide with the potential for diverse ecological adaptation. However, increasing salinization and changing climatic conditions are posing serious threats to the growth, yield, and quality of okra. Therefore, to mitigate increasing soil salinization and ensure sustainable okra production under rapidly changing climatic conditions, evaluation of new okra germplasm to develop salt tolerant cultivars is direly needed. The present study was designed to evaluate the genetic resources of okra genotypes for salt tolerance at growth and reproductive phases. Based on mophological and physio-biochemical responses of plants under stress condition, genotypes were divided into salt tolerant and succeptible groups. The experiment was comprised of 100 okra genotypes and each genotype was grown under control conditions and 6.5 dS m-1 NaCl concentration in a pot having 10 kg capacity. The experiment was conducted in a completely randomized design and each treatment was replicated three times. The results showed vast genetic variability among the evaluated okra germplasm traits like days to emergence, pod length, pod diameter, plant height, stem girth, and other yield-related parameters. Correlation analysis showed a highly significant positive association among the number of leaves at first flower and plant height at first flower.Likewise, pod weight also revealed a highly significant positive relationship for pod weight plant-1, pod length, and K+: Na+. Principal Component Analysis (PCA) revealed that out of 16 principal components (PCs), five components showed more than one eigenvalue and the first six PCs contributed 67.2% of the variation. Bi-plot analysis illustrated that genotypes 95, 111, 133, 99, and 128, under salt stress conditions, exhibited both high yield per plant and salt-tolerant behavior in other yield-related traits. On the basis of all studied traits, a salt susceptible group and a salt-tolerant group were formed. The salt tolerant group comprised of 97, 68, 95, 114, 64, 99, 111, 133, 128, and 109 genotypes, whereas, the salt susceptible group contained 137, 139, 130, 94, and 125 genotypes. Salt-tolerant okra genotypes were suggested to be used in further breeding programs aimed to develop salt tolerance in okra. These insights will empower precision breeding, underscore the importance of genetic diversity, and bear the potential to address the challenges of salt-affected soils while promoting broader agricultural resilience, economic prosperity, and food security.

Nano-Biochar Enhances Wheat Crop Productivity by Vindicating the Effects of Drought: In Relation to Physiological and Phenological Stages.

Climatic changes are major hindrances to crop productivity. Likewise, water scarcity is the major obstacle during different physiological and phenological stages, which ultimately reduces the wheat crop yield. So, there is a dire need to adopt modern approaches such as soil amendments, i.e., using nano-biochar (NBC) to boost soil health and wheat crop productivity. Therefore, a case study was performed in the wire house of the Agronomy Department, Faculty of Agriculture and Environmental Sciences, Islamia University of Bahawalpur. CRD (completely randomized design) with four treatments of NBC, i.e., NBC0 (control), NBC1 (0.5%), NBC2 (1.00%), NBC3 (1.5%), and four drought levels D0 = control, D1 = drought at tillering, D2 = drought at flowering, and D3 = drought at grain filling was used. The hypothesis for the case study was to investigate if the NBC increases crop productivity by boosting physiological and chemical attributes under different drought conditions at different phenological stages. Results showed that among NBC treatments, NBC2 (1.00%) showed 37.10% increase in peroxidase activity, 28.60% in superoxide dismutase, 63.33% in catalase, 22.03% in ascorbate peroxidase, and 6.66% in plant height as compared to other NBC treatments, whereas among drought treatments, D0 = control stood out in comparison to water deficit treatments at critical growth and development stages, statistically analyzed data revealed that D0 was able to generate plant height 6.17 times more, 12.76% in the number of grains per spike, 4.60% in osmotic potential, and 2.96% in stomatal conductance activities of wheat crop. D3 and NBC0 were identified as treatment levels with the statistically lowest growth and yield returns, respectively. It showed a decrease of 4.69% in leaf relative water contents, 12.33% in water potential, and 23.64% in fertile tillers. It was recommended that drought is avoided at any critical growth, particularly at the grain-filling stage. The use of organic substances (fertilizers) must be promoted as they possess soil and crop health-promoting properties and also reduce different management expenses (fertilizer cost). Using NBC helps boost crop growth in the presence of a limited water supply. However, extensive research is needed to find out the impact of these organic substances (humic acid, farmyard manure, and NBC) on different crops, particularly on wheat, under stress conditions.

Exploring the recuperative potential of brassinosteroids and nano-biochar on growth, physiology, and yield of wheat under drought stress.

Drought stress as a result of rapidly changing climatic conditions has a direct negative impact on crop production especially wheat which is the 2nd staple food crop. To fulfill the nutritional demand under rapidly declining water resources, there is a dire need to adopt a precise, and efficient approach in the form of different amendments. In this regard, the present study investigated the impact of nano-biochar (NBC) and brassinosteroids (BR) in enhancing the growth and productivity of wheat under different drought stress conditions. The field study comprised different combinations of amendments (control, NBC, BR, and NBC + BR) under three irrigation levels (D0, D1 and D2). Among different treatments, the synergistic approach (NBC + BR) resulted in the maximum increase in different growth and yield parameters under normal as well as drought stress conditions. With synergistic approach (NBC + BR), the maximum plant height (71.7 cm), spike length (17.1), number of fertile tillers m-2 (410), no. of spikelets spike-1 (19.1), no. of grains spike-1 (37.9), 1000 grain weight (37 g), grain yield (4079 kg ha-1), biological yield (10,502 kg ha-1), harvest index (43.5). In the case of physiological parameters such as leaf area index, relative water contents, chlorophyll contents, and stomatal conductance were maximally improved with the combined application of NBC and BR. The same treatment caused an increase of 54, 10, and 7% in N, P, and K contents in grains, respectively compared to the control treatment. Similarly, the antioxidant response was enhanced in wheat plants under drought stress with the combined application of NBC and BR. In conclusion, the combined application of NBC and BR caused a significant increase in the growth, physiological and yield attributes of wheat under drought stress.

Morpho-physiological and biochemical response of wheat to various treatments of silicon nano-particles under drought stress conditions.

Silicon nanoparticles (Si-NPs) have shown their potential for use in farming under water-deficient conditions. Thus, the experiment was accomplished to explore the impacts of seed priming of Si-NPs on wheat (Triticum aestivum L.) growth and yield under different drought levels. The plants were grown in pots under natural ecological environmental conditions and were harvested on 25th of April, 2020. The results revealed that seed priming of Si-NPs (0, 300, 600, and 900 mg/L) suggestively improved, the spike length, grains per spike, 1000 grains weight, plant height, grain yield, and biological yield by 12-42%, 14-54%, 5-49%, 5-41%, 17-62%, and 21-64%, respectively, relative to the control. The Si-NPs improved the leaf gas trade ascribes and chlorophyll a and b concentrations, though decreased the oxidative pressure in leaves which was demonstrated by the diminished electrolyte leakage and upgrade in superoxide dismutase and peroxidase activities in leaf under Si-NPs remedies over the control. The outcomes proposed that Si-NPs could improve the yield of wheat under a dry spell. In this manner, the utilization of Si-NPs by seed priming technique is a practical methodology for controlling the drought stress in wheat. These findings will provide the basis for future research and helpful to improve the food security under drought and heat related challenges.

Ameliorating Drought Effects in Wheat Using an Exclusive or Co-Applied Rhizobacteria and ZnO Nanoparticles.

Drought is a major abiotic factor and affects cereal-based staple food production and reliability in developing countries such as Pakistan. To ensure a sustainable and consistent food supply, holistic production plans involving the integration of several drought mitigation approaches are required. Using a randomized complete block design strategy, we examined the drought-ameliorating characteristics of plant growth-promoting rhizobacteria (PGPR) and nanoparticles (NPs) exclusively or as a combined application (T4) through three stages (D1, D2, and D3) of wheat growth (T1, control). Our field research revealed that Azospirillum brasilense alone (T2) and zinc oxide NPs (T3) improved wheat plant water relations, chlorophyll, proline, phenolics and grain quality, yield, and their allied traits over the stressed treatments. Specifically, the best outcome was observed in the combined treatment of PGPR and ZnO NPs (T4). Interestingly, the combined treatment delivered effective drought mitigation through enhanced levels of antioxidants (15% APX, 27% POD, 35% CAT, 38% PPO and 44% SOD) over controls at the grain-filling stage (GFS, D3 × T1). The 40% improvements were recorded under the combined treatment at GFS over their respective controls. Their combined usage (PGPR and ZnO NPs) was concluded as an effective strategy for building wheat resilience under drought, especially in arid and semi-arid localities.

Biochar enhances wheat crop productivity by mitigating the effects of drought: Insights into physiological and antioxidant defense mechanisms.

Drought stress is a major limitation in wheat production around the globe. Organic amendments could be the possible option in semi-arid climatic conditions to mitigate the adverse effects of drought at critical growth stages. Wheat straw biochar (BC0 = Control, BC1 = 3% biochar and BC2 = 5% biochar) was used to alleviate the drought stress at tillering (DTS), flowering (DFS), and grain filling (DGFS) stages. Drought stress significantly reduced the growth and yield of wheat at critical growth stages, with DGFS being the most susceptible stage, resulting in significant yield loss. Biochar application substantially reduced the detrimental effects of drought by improving plant height (15.74%), fertile tiller count (17.14%), spike length (16.61%), grains per spike (13.89%), thousand grain weight (10.4%), and biological yield (13.1%) when compared with the control treatment. Furthermore, physiological parameters such as water use efficiency (38.41%), stomatal conductance (42.76%), chlorophyll a (19.3%), chlorophyll b (22.24%), transpiration rate (39.17%), photosynthetic rate (24.86%), electrolyte leakage (-42.5%) hydrogen peroxide (-18.03%) superoxide dismutase (24.66%), catalase (24.11%) and peroxidase (-13.14%) were also improved by biochar application. The use of principal component analysis linked disparate scales of our findings to explain the changes occurred in wheat growth and yield in response to biochar application under drought circumstances. In essence, using biochar at 5% rate could be a successful strategy to promote wheat grain production by reducing the hazardous impacts of drought stress.

Assessing the potential of partial root zone drying and mulching for improving the productivity of cotton under arid climate.

Water scarcity constrains global cotton production. However, partial root-zone drying (PRD) and mulching can be used as good techniques to save water and enhance crop production, especially in arid regions. This study aimed to evaluate the effects of mulching for water conservation in an arid environment under PRD and to further assess the osmotic adjustment and enzymatic activities for sustainable cotton production. The study was carried out for 2 years in field conditions using mulches (NM = no mulch, BPM = black plastic mulch at 32 kg ha-1, WSM = wheat straw mulch at 3 tons ha-1, CSM = cotton sticks mulch at 10 tons ha-1) and two irrigation levels (FI = full irrigation and PRD (50% less water than FI). High seed cotton yield (SCY) achieved in FI+WSM (4457 and 4248 kg ha-1 in 2017 and 2018, respectively) and even in PRD+WSM followed by BPM>CSM>NM under FI and PRD for both years. The higher SCY and traits observed in FI+WSM and PRD+WSM compared with the others were attributed to the improved water use efficiency and gaseous exchange traits, increased hormone production (ABA), osmolyte accumulation, and enhanced antioxidants to scavenge the excess reactive oxygen. Furthermore, better cotton quality traits were also observed under WSM either with FI or PRD irrigation regimes. Mulches applications found effective to control the weeds in the order as BPM>WSM>CSM. In general, PRD can be used as an effective stratagem to save moisture along with WSM, which ultimately can improve cotton yield in the water-scarce regions under arid climatic regions. It may prove as a good adaptation strategy under current and future water shortage scenarios of climate change.